Abstract:
:P-450-male is a male specific form of cytochrome P-450 in rat liver microsomes. Cytochrome P-450 crossreactive with anti-P-450-male antibodies was purified to an electrophoretical homogeneity from liver microsomes of male beagle dogs. The specific content of the purified cytochrome P-450 (P-450-D1) was 16.9 nmol/mg protein. The apparent monomeric molecular weight of P-450-D1 was 48,000, which was smaller than P-450-male (51,000). P-450-D1 showed similarities in spectral properties, N-terminal amino acid sequence, and catalytic activities with some limited exceptions: P-450-D1 did not catalyze 2 alpha-hydroxylation of testosterone and progesterone and catalyzed 21-hydroxylation of progesterone. Based on these results, we propose that P-450-D1 is a form of cytochrome P-450 in the same gene subfamily as P-450-male.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Komori M,Shimada H,Miura T,Kamataki Tdoi
10.1016/0006-2952(89)90032-4subject
Has Abstractpub_date
1989-01-15 00:00:00pages
235-40issue
2eissn
0006-2952issn
1873-2968pii
0006-2952(89)90032-4journal_volume
38pub_type
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