Abstract：
OBJECTIVE:Endometrial carcinoma is the most common malignancy of the female genital tract. Therefore, there is an urgent need to understand the molecular mechanism of its metastasis. This study is aimed to explore the function and underlying mechanism of miR-326 in endometrial cancer (EC). PATIENTS AND METHODS:RT-PCR was used to evaluate the miR-326 expression in EC tissues and cell lines. The CKK-8 was used to detect the EC cells proliferation. Transwell assay was performed to evaluate the metastasis of EC cells. Targeted genes were predicted by a bioinformatics algorithm. Dual-luciferase reporter assays were performed to examine the regulation of putative miR-326 targets. The expression of TWIST1 and EMT-related proteins was assayed using Western blot. RESULTS:Our results proved that miR-326 expression was downregulated in EC cell lines and tissue samples. In vitro assays, our results indicated that over-expression of miR-326 inhibited cell proliferation, migration, invasion, and EMT. Moreover, Bioinformatics analysis revealed Twist homolog 1 (TWIST1), a putative tumor promoter, to be a potential target of miR-326. Results from a dual-luciferase reporter system supported TWIST1 as a direct target gene of miR-326. In addition, Western blot showed that over-expression of miR-326 resulted in decreased TWIST1 expression in EC cells. Final in vitro assays revealed that knockdown of TWIST1 inhibited EC cell, migration, invasion and EMT, suggesting that miR-326 exerted its tumor-suppressive role by targeting TWIST1. CONCLUSIONS:We demonstrate that miR-326 served as a tumor suppressor by targeting TWIST1, and may serve as a biomarker or therapeutic target for patients with EC.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Liu W,Zhang B,Xu N,Wang MJ,Liu Qsubject
Has Abstractpub_date
2017-10-01 00:00:00pages
3787-3793issue
17eissn
1128-3602issn
2284-0729journal_volume
21pub_type
杂志文章abstract：:Spider bites are not very common, especially in the Mediterranean area, and those affecting the ocular-palpebral region involving reconstructive surgery are particularly rare. In May 2010, the case of a Caucasian 24-year-old female patient was brought to the attention of the Dermatology Department, University of Cagli...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:A combination of comparative analysis of gene expression profiles between normal tissue samples and small cell lung cancer (SCLC) samples and network analysis was performed to identify key genes in SCLC. MATERIALS AND METHODS:Microarray data set GSE43346 was downloaded from Gene Expression Omnibus (GEO), inc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:In the latest years the use of lung ultrasound is increasing in the evaluation of pleural effusions, because it makes follow-up easier and drainage more efficient by providing guidance on the most appropriate sampling site. However, no standardized approach for ultrasound-guided thoracenthesis is actually ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect and mechanism of bone marrow stem cells (BMSCs) modified with CXCR-4 gene combined with stromal derived factor-1α (SDF-1α) in the treatment of acute spinal cord injury (SCI) in rats. MATERIALS AND METHODS:CXCR-4 gene was transfected by a virus. Spinal cord injury rats were randomly div...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：AIM:A method was developed to separate contaminant-free viable Toxoplasma gondii cysts from brain samples of infected mice for molecular biology studies and reinfection. MATERIALS AND METHODS:The mice brains were homogenized and washed with phosphate buffered saline (PBS) Tween 80 prior to fractionation using 19-22% ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:To explore the protective role of Vitamin D Receptor (VDR) in retinal ganglion cells (RGCs) after high-level glucose induction, and to investigate its underlying mechanism. MATERIALS AND METHODS:Primary RGCs were isolated from 24-hour-old Sprague Dawley (SD) rats and cultured in 50 mmol/L glucose. The expres...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201811_16292
更新日期：2018-11-01 00:00:00
abstract：INTRODUCTION:Bisphosphonates are considered as a first-line therapy for the prevention and treatment of osteoporosis, showing in double-blind, randomized, controlled trials a significant reduction of incidence of new vertebral fractures compared to placebo. Recently also, Denosumab has been shown to reduce the appearan...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,meta分析
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:Osteoporosis is a highly prevalent disease worldwide. Consequences of vertebral osteoporotic fractures include pain and progressive vertebral collapse resulting in spinal kyphosis, decreased quality of life, disability and mortality. Minimally invasive procedures represent an advance to the treatment of osteo...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:This review paper aims to summarize the current state of knowledge on the role of the pneumologist in the diagnosis and respiratory treatment of children affected by obstructive Sleep Disordered Breathing (SDB). MATERIALS AND METHODS:A literature review has been performed on the following topics: obstructive...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_201903_17342
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore whether miR-650 could inhibit the proliferation of glioma by regulating FAM83F and to investigate the specific role of miR-650 in glioma occurrence. PATIENTS AND METHODS:The expression of FAM83F in tumor or para-cancerous tissues of 24 glioma patients was detected by quan...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201812_16537
更新日期：2018-12-01 00:00:00
abstract：:Sarcoidosis is a granulomatous disease of unknown origin, with pulmonary findings in more than 90% of patients. Extrapulmonary involvement is common and all organs can be involved (especially lymph nodes, eyes, joints, central nervous system) but it is rare to find an isolated extrapulmonary disease (less than 10% of ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2009-03-01 00:00:00
abstract：OBJECTIVE:Lung cancer is one of the most common malignancies worldwide, the morbidity and mortality of which have been on rising in recent years. Moreover, lncRNAs have been implicated in the development of various cancers, as well as cancer treatment and prognosis. In this study, long non-coding RNA (lncRNA) MEG3, an ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201802_14384
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVES:Control of postoperative symptoms is of paramount importance in proctologic surgery. Phlebotropic activity, protective effect on the capillaries and anti-inflammatory properties of the flavonoids have been reported in several studies. They have been used to treat a variety of conditions including chronic ven...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2014-01-01 00:00:00
abstract：AIM:To study the Health Related Quality of Life (HRQoL) and metabolic assessment in 33 children affected with type 1 diabetes (18 males, 15 females; mean age 10.3 years). METHODS AND RESULTS:We used the Child Health Questionnaire-Parental Form 50 items (CHQ-PF50), measurements of metabolic control and we related them ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore the role of HOTAIR in inflammatory response after acute myocardium infarction (AMI) and to investigate its underlying mechanism. MATERIALS AND METHODS:The AMI model was first constructed in rats, and heart tissues were harvested. Expression levels of HOTAIR and receptor o...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201811_16282
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:Hydrocarbon pneumonia is distinct among the types of childhood pneumonia in that it has a different pathogenesis and treatment and is preventable. In this study, the cases of 54 children with hydrocarbon pneumonia admitted to the Dicle University Medical Faculty Pediatric Chest Diseases Unit between the years...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:Ulcerative colitis (UC) is a kind of chronic inflammatory bowel diseases that seriously endangers human health. The pathogenesis of UC is closely related to the intestinal immune response. Cytokines exert a key role in the regulation of intestinal inflammatory and immune responses. Abnormalities in the functi...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201808_15633
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVE:The purpose of this study is to bring attention to a case of acute encephalitis not concurrent with acute cerebellitis. CASE PRESENTATION:Five days after onset of common cold symptoms, a 17-months-old girl suffered convulsions, vomiting and respiratory arrest. On exam, she had pharyngeal inflammation, brisk ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202010_23430
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND:Sublingual-specific immunotherapy (SLIT) is considered as a valid treatment of respiratory allergies. AIM:We performed a case-control study to evaluate the effect of SLIT in children with allergic asthma and rhinitis. PATIENTS AND METHODS:The study plan included 140 patients (age 6-14 yr, 43% girls and 57%...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：OBJECTIVE:This study was aimed to explore the role of long noncoding RNA (lncRNA) AB073614 and its potential mechanisms in the glioma development. MATERIALS AND METHODS:Expression levels of lncRNA AB073614 in glioma tissues and glioma cell lines were evaluated by quantitative real-time PCR (qRT-PCR). MTT assays were c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of miR-519d on the 5-fluorouracil resistance in colorectal cancer cells and to explore the mechanism. MATERIALS AND METHODS:Colorectal cancer cells HCT116 and SW480 were transfected with miR519d-mimic or siCCND1 by transient transfection. The sensitivity of cells to 5-Fu was assaye...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201805_14989
更新日期：2018-05-01 00:00:00
abstract：:Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long noncoding RNA UCA1 promotes proliferation and metastasis of thyroid cancer cells by sponging miR-497-3p, by H. Gao, J.-Y. Yang, L.-X. Tong, H. Jin, C...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202007_22203
更新日期：2020-07-01 00:00:00
abstract：OBJECTIVE:Rheumatic heart disease (RHD) is a serious complication of rheumatic fever (RF). Plasma homocysteine (Hcy) levels are increased in RHD patients. MTHFR catalyzes the irreversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate and plays a vital role in Hcy metabolism. We hypothesize th...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:To determine the expression of neutrophil gelatinase-associated lipocalin (NGAL) and vascular endothelial growth factor (VEGF) in endometrial carcinoma. PATIENTS AND METHODS:The clinical features (FIGO staging, pathological type, differentiation level, myometrial invasion depth and tumor size) and immunostai...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201802_14287
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:Orolingual angioedema (OA) is a rare clinical complication with a potentially fatal risk that occurs after the intravenous application of alteplase (rt-PA) in patients with acute ischemic stroke. The purpose of this work is to investigate the related factors of OA in patients with acute ischemic stroke after ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201803_14496
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:To explore the possible role and mechanism of miR-497 in cutaneous squamous cell carcinoma. PATIENTS AND METHODS:Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect miR-497 and FAM114A2 expression level in 38 cases of cutaneous squamous cell carcinoma (CSCC) and 22 normal skin tissu...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201811_16272
更新日期：2018-11-01 00:00:00
abstract：:Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "The effect of miR-224 down-regulation on SW80 cell proliferation and apoptosis and weakening of ADM drug resistance, by C.-Q. Liang, Y.-M. Fu, Z.-Y. Liu, ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202007_22247
更新日期：2020-07-01 00:00:00
abstract：:Despite a decline in the incidence in Western countries, gastric cancer is still the second most common cause of cancer-related death worldwide. Many advances have been made in diagnosis and treatment of gastric cancer in the last decades but the prognosis for gastric cancer patients remains disappointing, especially ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2010-04-01 00:00:00
abstract：:The neurogenic dysfunctions of the detrusor and the sphincter are caused by either a known congenital defect of the nervous system or by acquired damage to the nervous system. In patients with idiopathic bladder dysfunctions neurological examinations fail to reveal any pathology in the nervous system. The treatment st...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:Colonoscopy is recognized as the primary screening test for colorectal cancer. However, its inaccuracy in identifying the exact tumor localization is still high. As a consequence, repeated colonoscopies and changes in the surgical management have been reported. This study aims to evaluate the quality of 216 c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201903_17401
更新日期：2019-03-01 00:00:00
