Evidence for a uniformly small isotope effect of nitrogen leaching loss: results from disturbed ecosystems in seasonally dry climates.

Abstract:

:Nitrogen (N) losses constrain rates of plant carbon dioxide (CO2) uptake and storage in many ecosystems globally. N isotope models have been used to infer that ~30 % of terrestrial N losses occur via microbial denitrification; however, this approach assumes a small isotope effect associated with N leaching losses. Past work across tropical/sub-tropical forest sites has confirmed this expectation; however, the stable N isotope ratio (δ(15)N) of ecosystem leaching has yet to be systematically evaluated in seasonally dry climates or across major ecosystem disturbances. We here present new measurements of the δ(15)N of total dissolved N (TDN) in small streams, bulk deposition, and soil pools across eight watershed sites in California, including grassland, chaparral, and coastal redwood forest ecosystems, with and without fire, grazing, and forest harvesting. Regardless of the dominant vegetation type or disturbance regime, average δ(15)N of TDN in stream water differed only slightly (<~1 ‰) from that of bulk soil δ(15)N, revealing a uniformly small isotope effect associated with N leaching losses even under non-steady state conditions. Rather, lower input δ(15)N compared to TDN δ(15)N in streams pointed to fractionations via gaseous loss pathways as the dominant mechanism behind soil δ(15)N enrichment. We conclude that N leaching does not impart a major isotope effect across a broad range of ecosystems and conditions examined, thereby advancing the N gas-loss hypothesis as the principal explanation for variation in bulk soil δ(15)N.

journal_name

Oecologia

journal_title

Oecologia

authors

Mnich ME,Houlton BZ

doi

10.1007/s00442-015-3433-0

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

323-33

issue

2

eissn

0029-8549

issn

1432-1939

pii

10.1007/s00442-015-3433-0

journal_volume

181

pub_type

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