Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum-Phaseolus vulgaris interaction.

Abstract:

:The fungus Sclerotinia sclerotiorum (Lib.) de Bary, one of the most important plant pathogens, causes white mold on a wide range of crops. Crop yield can be dramatically decreased due to this disease, depending on the plant cultivar and environmental conditions. In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Additionally, the temporal expression profile obtained by RT-qPCR was evaluated for the following categories of plant defense-related genes: pathogenesis-related genes (PvPR1, PvPR2, and PvPR3), phenylpropanoid pathway genes (PvIsof, PvFPS1, and 4CL), and genes involved in defense and stress-related categories (PvLox, PvHiprp, PvGST, PvPod, and PvDox). Data obtained in this study provide a starting point for achieving a better understanding of the pathosystem S. sclerotiorum-P. vulgaris.

journal_name

Front Microbiol

authors

Oliveira MB,de Andrade RV,Grossi-de-Sá MF,Petrofeza S

doi

10.3389/fmicb.2015.01162

subject

Has Abstract

pub_date

2015-10-26 00:00:00

pages

1162

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Identification of Genes Essential for Antibiotic-Induced Up-Regulation of Plasmid-Transfer-Genes in Cephalosporin Resistant Escherichia coli.

    abstract::Bacterial conjugation is one of the most important mechanisms for spread of antibiotic resistance among bacteria. We have previously demonstrated that cefotaxime (CTX) exposure up-regulates expression of Type-IV conjugation transfer genes, and that this leads to increased transfer of a blaCTX-M-1 encoding IncI1 resist...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02203

    authors: Liu G,Olsen JE,Thomsen LE

    更新日期:2019-09-24 00:00:00

  • 1H-NMR-Based Endometabolome Profiles of Burkholderia cenocepacia Clonal Variants Retrieved from a Cystic Fibrosis Patient during Chronic Infection.

    abstract::During cystic fibrosis (CF) chronic lung infections, bacteria of the Burkholderia cepacia complex (Bcc) are exposed for several years to a stressful and changing environment. These environmental challenges results in genetic changes of the initial infecting strain with the consequent diversification of genotypes and p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02024

    authors: Moreira AS,Lourenço AB,Sá-Correia I

    更新日期:2016-12-20 00:00:00

  • Molecular Detection of Azole-Resistant Aspergillus fumigatus in Clinical Samples.

    abstract::Aspergillus diseases are often caused by Aspergillus fumigatus. Azoles are the mainstay of therapy, but the management of aspergillosis is hampered by the emergence of azole resistance. Rapid detection of azole resistance might benefit treatment outcome by early treatment modifications. However, the yield of fungal cu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00515

    authors: Buil JB,Zoll J,Verweij PE,Melchers WJG

    更新日期:2018-03-21 00:00:00

  • Catabolism of Alkylphenols in Rhodococcus via a Meta-Cleavage Pathway Associated With Genomic Islands.

    abstract::The bacterial catabolism of aromatic compounds has considerable promise to convert lignin depolymerization products to commercial chemicals. Alkylphenols are a key class of depolymerization products whose catabolism is not well-elucidated. We isolated Rhodococcus rhodochrous EP4 on 4-ethylphenol and applied genomic an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01862

    authors: Levy-Booth DJ,Fetherolf MM,Stewart GR,Liu J,Eltis LD,Mohn WW

    更新日期:2019-08-20 00:00:00

  • Global Transcriptomic Changes Induced by Infection of Cucumber (Cucumis sativus L.) with Mild and Severe Variants of Hop Stunt Viroid.

    abstract::Fifteen years after transfer to hops, hop stunt viroid-grapevine (HSVd-g) was replaced by HSVd-hop (HSVd-h), a sequence variant that contains changes at five different positions. HSVd-g54 is a laboratory mutant derived from HSVd-g that differs from its progenitor by a single G to A substitution at position 54. While i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02427

    authors: Xia C,Li S,Hou W,Fan Z,Xiao H,Lu M,Sano T,Zhang Z

    更新日期:2017-12-12 00:00:00

  • Bacterial Catabolism of Dimethylsulfoniopropionate (DMSP).

    abstract::Dimethylsulfoniopropionate (DMSP) is a metabolite produced primarily by marine phytoplankton and is the main precursor to the climatically important gas dimethylsulfide (DMS). DMS is released upon bacterial catabolism of DMSP, but it is not the only possible fate of DMSP sulfur. An alternative demethylation/demethiola...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00172

    authors: Reisch CR,Moran MA,Whitman WB

    更新日期:2011-08-12 00:00:00

  • Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios.

    abstract::Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02067

    authors: Wegner KM,Piel D,Bruto M,John U,Mao Z,Alunno-Bruscia M,Petton B,Le Roux F

    更新日期:2019-09-06 00:00:00

  • Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures.

    abstract::Milk spoilage is caused by the presence of proteolytic enzymes produced by Pseudomonas spp. during storage at low temperatures. The aim of this study was to identify Pseudomonas spp. in raw milk and investigate their associated proteolytic properties at low temperatures. Raw milk samples (n = 87) were collected from 8...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02158

    authors: Meng L,Zhang Y,Liu H,Zhao S,Wang J,Zheng N

    更新日期:2017-11-08 00:00:00

  • Interactions Between Clostridioides difficile and Fecal Microbiota in in Vitro Batch Model: Growth, Sporulation, and Microbiota Changes.

    abstract::Disturbance in gut microbiota is crucial for the development of Clostridioides difficile infection (CDI). Different mechanisms through which gut microbiota influences C. difficile colonization are known. However, C. difficile could also affect gut microbiota balance as previously demonstrated by cultivation of fecal m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01633

    authors: Horvat S,Rupnik M

    更新日期:2018-07-24 00:00:00

  • Protection Mechanism of Clostridium butyricum against Salmonella Enteritidis Infection in Broilers.

    abstract::This study was designed to evaluate the protection mechanism of oral administration of Clostridium butyricum against Salmonella enteritidis (SE) colonization in broilers. In the current study, 180 one-day-old healthy Arbor Acres (AA) broilers were meanly grouped into three, with three replicates of 20 birds each. An n...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01523

    authors: Zhao X,Yang J,Wang L,Lin H,Sun S

    更新日期:2017-08-09 00:00:00

  • Bacillus amyloliquefaciens Ameliorates Dextran Sulfate Sodium-Induced Colitis by Improving Gut Microbial Dysbiosis in Mice Model.

    abstract::Several Bacillus strains exert beneficial effects on the maintenance of intestinal homeostasis and host health. However, whether Bacillus amyloliquefaciens (BA) can improve gut microbial dysbiosis and ameliorate colitis is unknown. Therefore, we conducted the present study to investigate the effects of BA administrati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03260

    authors: Cao G,Wang K,Li Z,Tao F,Xu Y,Lan J,Chen G,Yang C

    更新日期:2019-01-08 00:00:00

  • Novel Screen to Assess Bactericidal Activity of Compounds Against Non-replicating Mycobacterium abscessus.

    abstract::Mycobacterium abscessus infections are increasing worldwide. Current drug regimens are largely ineffective, yet the current development pipeline for M. abscessus is alarmingly sparse. Traditional discovery efforts for M. abscessus assess the capability of a new drug to inhibit bacterial growth under nutrient-rich grow...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02417

    authors: Berube BJ,Castro L,Russell D,Ovechkina Y,Parish T

    更新日期:2018-10-10 00:00:00

  • Proteogenomic Analysis of Epibacterium Mobile BBCC367, a Relevant Marine Bacterium Isolated From the South Pacific Ocean.

    abstract::Epibacterium mobile BBCC367 is a marine bacterium that is common in coastal areas. It belongs to the Roseobacter clade, a widespread group in pelagic marine ecosystems. Species of the Roseobacter clade are regularly used as models to understand the evolution and physiological adaptability of generalist bacteria. E. mo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03125

    authors: Matallana-Surget S,Werner J,Wattiez R,Lebaron K,Intertaglia L,Regan C,Morris J,Teeling H,Ferrer M,Golyshin PN,Gerogiorgis D,Reilly SI,Lebaron P

    更新日期:2018-12-21 00:00:00

  • Further Spread of a blaKPC-Harboring Untypeable Plasmid in Enterobacteriaceae in China.

    abstract::The wide spread of Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae is great threat to public health in China. Plasmids are among the major factors mediating blaKPC gene dissemination. A total of 156 carbapenem-resistant Enterobacteriaceae (CRE) isolates were identified in a tertiary hospital in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01938

    authors: Huang J,Ding H,Shi Y,Zhao Y,Hu X,Ren J,Huang G,Wu R,Zhao Z

    更新日期:2018-08-21 00:00:00

  • Understanding the Effects of High Pressure on Bacterial Spores Using Synchrotron Infrared Spectroscopy.

    abstract::Bacterial spores are extremely resistant life-forms that play an important role in food spoilage and foodborne disease. The return of spores to a vegetative cell state is a three-step process, these being activation, germination, and emergence. High-pressure (HP) processing is known to induce germination in part of th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03122

    authors: Modugno C,Peltier C,Simonin H,Dujourdy L,Capitani F,Sandt C,Perrier-Cornet JM

    更新日期:2020-01-31 00:00:00

  • The Role of Volatile Organic Compounds and Rhizosphere Competence in Mode of Action of the Non-pathogenic Fusarium oxysporum FO12 Toward Verticillium Wilt.

    abstract::Verticillium wilts caused by Verticillium spp. are among the most challenging plant diseases to control and affect numerous hosts worldwide. Due to the lack of effective, conventional control methods, integrated control strategies provide a promising approach to manage these diseases. The non-pathogenic Fusarium oxysp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01808

    authors: Mulero-Aparicio A,Cernava T,Turrà D,Schaefer A,Di Pietro A,López-Escudero FJ,Trapero A,Berg G

    更新日期:2019-08-02 00:00:00

  • Antimicrobial food packaging: potential and pitfalls.

    abstract::Nowadays food preservation, quality maintenance, and safety are major growing concerns of the food industry. It is evident that over time consumers' demand for natural and safe food products with stringent regulations to prevent food-borne infectious diseases. Antimicrobial packaging which is thought to be a subset of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00611

    authors: Malhotra B,Keshwani A,Kharkwal H

    更新日期:2015-06-16 00:00:00

  • Role of Two-Component System Response Regulator bceR in the Antimicrobial Resistance, Virulence, Biofilm Formation, and Stress Response of Group B Streptococcus.

    abstract::Group B Streptococcus (GBS; Streptococcus agalactiae) is a leading cause of sepsis in neonates and pregnant mothers worldwide. Whereas the hyper-virulent serogroup III clonal cluster 17 has been associated with neonatal disease and meningitis, serogroup III ST283 was recently implicated in invasive disease among non-p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00010

    authors: Yang Y,Luo M,Zhou H,Li C,Luk A,Zhao G,Fung K,Ip M

    更新日期:2019-01-23 00:00:00

  • Genomic and Transcriptomic Insights into Calcium Carbonate Biomineralization by Marine Actinobacterium Brevibacterium linens BS258.

    abstract::Calcium carbonate (CaCO3) biomineralization has been investigated due to its wide range of scientific and technological implications, however, the molecular mechanisms of this important geomicrobiological process are largely unknown. Here, a urease-positive marine actinobacterium Brevibacterium linens BS258 was demons...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00602

    authors: Zhu Y,Ma N,Jin W,Wu S,Sun C

    更新日期:2017-04-06 00:00:00

  • Differential NtcA Responsiveness to 2-Oxoglutarate Underlies the Diversity of C/N Balance Regulation in Prochlorococcus.

    abstract::Previous studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02641

    authors: Domínguez-Martín MA,López-Lozano A,Clavería-Gimeno R,Velázquez-Campoy A,Seidel G,Burkovski A,Díez J,García-Fernández JM

    更新日期:2018-01-09 00:00:00

  • Prioritized Expression of BDH2 under Bulk Translational Repression and Its Contribution to Tolerance to Severe Vanillin Stress in Saccharomyces cerevisiae.

    abstract::Vanillin is a potent fermentation inhibitor derived from the lignocellulosic biomass in biofuel production, and high concentrations of vanillin result in the pronounced repression of bulk translation in Saccharomyces cerevisiae. Studies on genes that are efficiently translated even in the presence of high concentratio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01059

    authors: Ishida Y,Nguyen TT,Kitajima S,Izawa S

    更新日期:2016-07-06 00:00:00

  • PromA Plasmids Are Instrumental in the Dissemination of Linuron Catabolic Genes Between Different Genera.

    abstract::PromA plasmids are broad host range (BHR) plasmids, which are often cryptic and hence have an uncertain ecological role. We present three novel PromA γ plasmids which carry genes associated with degradation of the phenylurea herbicide linuron, two of which originated from unrelated Hydrogenophaga hosts isolated from d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00149

    authors: Werner J,Nour E,Bunk B,Spröer C,Smalla K,Springael D,Öztürk B

    更新日期:2020-02-18 00:00:00

  • Role of Dilution Rate and Nutrient Availability in the Formation of Microbial Biofilms.

    abstract::We revisited the mathematical model of the chemostat and examined consequences of considerably decreasing the concentration of limiting nutrient in the inflow for the growth of both the planktonic and biofilm cells in the chemostat tank (fermenter). The model predicts a substantially lower steady-state biomass of plan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00916

    authors: Legner M,McMillen DR,Cvitkovitch DG

    更新日期:2019-04-30 00:00:00

  • Effects of Cultured Root and Soil Microbial Communities on the Disease of Nicotiana tabacum Caused by Phytophthora nicotianae.

    abstract:Background:Black shank, caused by the oomycete pathogen Phytophthora nicotianae, is responsible for huge economic losses worldwide. Research has focused on biocontrol to prevent disease and to minimize the use of synthetic fungicides. Methods:We explored and compared the efficacy of suppressive microflora cultured fro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00929

    authors: Liu T,Xiao Y,Yin J,Yi T,Zhou Z,Hsiang T,Tang Q,Chen W

    更新日期:2020-05-15 00:00:00

  • A bioassay for the detection of benzimidazoles reveals their presence in a range of environmental samples.

    abstract::Cobamides are a family of enzyme cofactors that include vitamin B12 (cobalamin) and are produced solely by prokaryotes. Structural variability in the lower axial ligand has been observed in cobamides produced by diverse organisms. Of the three classes of lower ligands, the benzimidazoles are uniquely found in cobamide...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00592

    authors: Crofts TS,Men Y,Alvarez-Cohen L,Taga ME

    更新日期:2014-11-13 00:00:00

  • Offspring Microbiomes Differ Across Breeding Sites in a Panmictic Species.

    abstract::High dispersal rates are known to homogenize host's population genetic structure in panmictic species and to disrupt host local adaptation to the environment. Long-distance dispersal might also spread micro-organisms across large geographical areas. However, so far, to which extent selection mechanisms that shape host...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00035

    authors: Gillingham MAF,Béchet A,Cézilly F,Wilhelm K,Rendón-Martos M,Borghesi F,Nissardi S,Baccetti N,Azafzaf H,Menke S,Kayser Y,Sommer S

    更新日期:2019-02-06 00:00:00

  • Beneficial Effects of Spices in Food Preservation and Safety.

    abstract::Spices have been used since ancient times. Although they have been employed mainly as flavoring and coloring agents, their role in food safety and preservation have also been studied in vitro and in vivo. Spices have exhibited numerous health benefits in preventing and treating a wide variety of diseases such as cance...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01394

    authors: Gottardi D,Bukvicki D,Prasad S,Tyagi AK

    更新日期:2016-09-21 00:00:00

  • Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of Old Cryptococcus neoformans Cells.

    abstract::As Cryptococcus neoformans mother cells generationally age, their cell walls become thicker and cell-wall associated virulence factors are upregulated. Antiphagocytic protein 1 (App1), and laccase enzymes (Lac1 and Lac2) are virulence factors known to contribute to virulence of C. neoformans during infection through i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02513

    authors: Orner EP,Bhattacharya S,Kalenja K,Hayden D,Del Poeta M,Fries BC

    更新日期:2019-11-07 00:00:00

  • Time-Resolved Transcriptomics and Constraint-Based Modeling Identify System-Level Metabolic Features and Overexpression Targets to Increase Spiramycin Production in Streptomyces ambofaciens.

    abstract::In this study we have applied an integrated system biology approach to characterize the metabolic landscape of Streptomyces ambofaciens and to identify a list of potential metabolic engineering targets for the overproduction of the secondary metabolites in this microorganism. We focused on an often overlooked growth p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00835

    authors: Fondi M,Pinatel E,Talà A,Damiano F,Consolandi C,Mattorre B,Fico D,Testini M,De Benedetto GE,Siculella L,De Bellis G,Alifano P,Peano C

    更新日期:2017-05-12 00:00:00

  • Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay.

    abstract::Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow im...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.596005

    authors: Shi L,Xu L,Xiao R,Zhou Z,Wang C,Wang S,Gu B

    更新日期:2020-11-06 00:00:00