Abstract:
AIM:To establish the specific features of the taxonomic and functional composition of the enteric microbiota in patients with alcoholic liver cirrhosis (LC). SUBJECTS AND METHODS:Metagenomic analysis was used to study the taxonomic composition and functional potential of the enteric microbiota in 20 patients with alcoholic LC. Total DNA was isolated from the patients' fecal samples; thereafter full genome sequencing was carried out. The metagenomic analysis yielded the results of the relative taxonomic and functional abundance of microbial species in the test samples. These were comparatively analyzed with the previously published metagenomic datasets of healthy population cohorts in the Russian Federation, as well as in Denmark, China, and the USA. RESULTS:In the majority of patients, the dominant part of the intestinal community represented bacterial species constituting the normal human intestinal flora. At the same time, abnormal gut microbiota composition, which was suggestive of marked dysbacteriosis, was identified in a number of patients. In addition, pooled analysis of the data could identify a number of species with a statistically significantly increase and decrease in the relative abundance as compared to the control groups. Thus, the enteric microbiota of the patients with alcoholic LC showed a high proportion of bacteria characteristic of the oral cavity. Analysis of the pooled metabolic potential of the microbiota in these patients demonstrated the higher abundance of enzyme genes involved in alcohol metabolism. CONCLUSION:In the patients with alcoholic LC, the microbiota composition changes identified in individual bacterial species may be associated with gastrointestinal comorbidities, such as chronic erosive gastritis, chronic pancreatitis, and gastric ulcer. The alterations occurring in alcoholic cirrhosis promote the penetration and generation of oral cavity-specific microorganisms in the human intestine. This may a potential biomarker for the diagnosis of liver diseases. The bacterial enzyme genes involved in alcohol metabolism have an increased abundance in patients with alcoholic LC and healthy volunteers from the Russian Federation. :Цель исследования. Установление особенностей таксономического и функционального состава микробиоты кишечника у пациентов с циррозом печени (ЦП) алкогольной этиологии. Материалы и методы. С помощью метагеномного анализа исследован таксономический состав и функциональный потенциал микробиоты кишечника у 20 пациентов с ЦП алкогольной этиологии. Из образцов кала пациентов выделена тотальная ДНК, после чего проведено полногеномное секвенирование. В ходе анализа метагеномных данных получены результаты по относительной таксономической и функциональной представленности микробных видов в исследуемых образцах. Проведен сравнительный анализ с ранее опубликованными наборами метагеномных данных по когортам здорового населения Российской Федерации, а также Дании, Китая и США. Результаты. У большинства пациентов преобладающая часть кишечного сообщества представлена бактериальными видами, составляющими нормофлору кишечника человека. В то же время у ряда пациентов идентифицирован аномальный состав микробиоты кишечника, свидетельствующий о выраженном дисбактериозе. Дополнительно совокупный анализ данных позволил идентифицировать ряд видов со статистически значимо повышенной и пониженной относительной представленностью по сравнению с контрольными группами. Так, в микробиоте кишечника пациентов с ЦП алкогольной этиологии наблюдается повышенное содержание бактерий, характерных для ротовой полости. Анализ совокупного метаболического потенциала микробиоты таких пациентов продемонстрировал повышенную представленность генов ферментов, участвующих в метаболизме этанола (алкоголя). Заключение. Идентифицированные по отдельным бактериальным видам изменения состава микробиоты у пациентов с алкогольным ЦП могут быть связаны с сопутствующими заболеваниями желудочно-кишечного тракта, таких как хронический эрозивный гастрит, хронический панкреатит и язвенная болезнь желудка. Изменения, происходящие при алкогольном циррозе, способствуют проникновению и размножению в кишечнике человека микроорганизмов, характерных для ротовой полости. Это может быть потенциальным биомаркером для диагностики заболеваний печени. Бактериальные гены ферментов, участвующие в метаболизме этанола, обладают повышенной представленностью как в группе пациентов с алкогольным ЦП, так и у здоровых добровольцев из Российской Федерации.
journal_name
Ter Arkhjournal_title
Terapevticheskii arkhivauthors
Shalikiani NV,Bakulin IG,Dubinkina VB,Ishchenko DS,Alexeev DG,Tyakht AV,Pavlenko AV,Ilyina EN,Kostryukova ES,Taraskina AE,Skorodumova LO,Maev IV,Govorun VMdoi
10.17116/terarkh2015871259-65subject
Has Abstractpub_date
2015-01-01 00:00:00pages
59-65issue
12eissn
0040-3660issn
2309-5342journal_volume
87pub_type
杂志文章abstract::Exudative cholesterol xanthomatous pericarditis was found at autopsy of a patient aged 57 who had suffered from the edematous form of thrombophlebitis of the deep crural veins complicated by thromboses of the iliac veins, vena cava inferior, recurring thromboembolism of the pulmonary artery, and pulmonary infarctions....
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::A possibility of life-time fibrinolysis activation with blast cells was studied in 54 patients with different variants of acute leukemia. The study showed that practically in all the variants of acute leukemia the content of fibrinolysis activators in the patients' blood was elevated. Active proteolytic enzymes of non...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:AIM:To evaluate clinical implications of measurements of the level of soluble cell molecules of adhesion (sCMA) in systemic lupus erythematosus (SLE) with antiphospholipid syndrome (APS) and primary APS (PAPS). MATERIAL AND METHODS:Serum levels of sP-selectine, sE-selectine, sVCAM-1 were determined with enzyme immunoa...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::This article presents evidence of the prevalence of gastroesophageal reflux disease (GERD) and highlights its main treatment options. Among its medications, particular emphasis is laid on alginates and their main mechanisms of action are described. There is information on the efficacy of alginates, including the algin...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:10.17116/terarkh2015875118-121
更新日期:2015-01-01 00:00:00
abstract::In the article, we report the causes of pulmonary hemorrhage (PH) according to the literature data and own experience, with an emphasis on patients suffering from rheumatic diseases. Methods of diagnosis and modern approaches to the treatment of PH are analyzed. :В представленной статье на основе данных литературы и ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/00403660.2019.05.000234
更新日期:2019-05-15 00:00:00
abstract:AIM:To evaluate occurrence of DNA-abzymes with catalytic (DNA-hydrolysing) and cytotoxic properties in patients with systemic lupus erythematosus (SLE) for examination of clinical value of DNA-abzymes in diagnosis of autoimmune syndrome and apoptosis level in different variants of immunopathology. MATERIAL AND METHODS...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,多中心研究
doi:
更新日期:2001-01-01 00:00:00
abstract::The peculiarities of prevalence of coronary heart disease (CHD), its main risk factors and their interrelationships were studied on the basis of the results of epidemiological surveys of an organized population of men aged 20 to 59 engaged in research, and an unorganized male population in one of Moscow districts. A s...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Annually 0.5-1 million people die from terminal liver injuries or hepatocellular carcinoma, which are associated with hepatitis B virus (HBV); 5-10% of liver transplantations are performed due to the outcomes of HBV infections. All patients with liver cirrhosis in the outcome of replication-phase chronic hepatitis B n...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00
abstract::The authors describe the results of a study into antioxidants (superoxide dismutase and catalase) and production of the active forms of oxygen (superoxide anion radical, hydrogen peroxide, hydroxyl radical, and singlet oxygen) by blood and bone marrow leukocytes from patients with Fanconi's anemia. The generation of t...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Study of changes in the electrocardiogram during spontaneous attacks of angina pectoris has shown that out of 80 patients examined, 78 (98.6%) patients with angina pectoris at rest manifested ischemic-type changes in the end part of the ventricular complex of the ECG, whereas in 81% of the patients, rhythm and conduct...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::A study was made of the periodical motor activity (PMA) of the antral part of the stomach (APS) and duodenum (D) using a manometric system of 4 continuously perfused catheters in 96 patients with peptic ulcer, 20 patients with ulcer of the body of the stomach, 26--with chronic pancreatitis, and in 16 healthy controls....
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::New evidence is reviewed on efficacy and safety of chimeric monoclonal antibodies to B-cell CD20 molecule (rituximab) in the treatment of rheumatoid arthritis. ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
abstract::The paper provides current views on the structure and functional activity of bacterial toxins, clinical use of bacterial lipopolysaccharides and their derivatives as immunostimulators, review investigations on the introduction of the botulinic toxin A in ophthalmological and neurological practice, on production of imm...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章,评审
doi:
更新日期:1994-01-01 00:00:00
abstract:AIM:To examine structural and electrophysiological predictors of the onset and establishment as chronic of cardiac fibrillation (CF). MATERIAL AND METHODS:Echocardiography, transesophageal electrophysiological examination of the heart were performed in 420 cases of paroxysmal cardiac fibrillation (PCF). They were diag...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:AIM:To study effects of long-term morphine-clofelin epidural analgesia and infusion of nitroglycerin on formation of the necrosis focus and the clinical course of acute myocardial infarction (MI). MATERIAL AND METHODS:Ninety patients were examined within the first 12 hours of anterior macrofocal MI. Forty six patients...
journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:AIM:The aim of the study was to study the effectiveness of the complex use of bacterial therapeutic vaccine Immunovac-VP4 and allergen-specific immune therapy (ASIT) in pollinosis in children and adults. MATERIALS AND METHODS:Bacterial therapeutic vaccine Immunovac-VP4 was used annually, nasal and oral administration ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.26442/terarkh201890316-20
更新日期:2018-04-19 00:00:00
abstract::Short-term results of rehabilitation of 1046 CHD patients at a cardiological sanatorium in mountains at average altitude (1600 m above the sea level) were studied. Large focal myocardial infarction (MI) was found in 738 of them, small focal MI in 249, and acute focal myocardial dystrophy of ischemic genesis in 59. Of ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Fourteen patients on hemosorption therapy were studied for variations in functional activity of neutrophils isolated from peripheral blood. These variations were evaluated from neutrophil chemiluminescence during adhesion reflecting the generation of active forms of oxygen. A correlation was established between the po...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Rheumon-Gel was used for therapy of 51 patients with rheumatoid arthritis. It has been shown that the drug has a marked antiinflammatory and analgesic action, a positive effect on a number of subjective (pain in the joints and constrained movements in the morning), objective (body temperature, edema and joint function...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:AIM:To compare programs of chemotherapy used in adult Berkitt-like lymphoma (ABLL); to assess efficacy and toxicity of the protocol AblL-M-04. MATERIAL AND METHODS:31 ABLL patients (23 males, 8 females, mean age 27 years) participated in the study performed in Hematological Research Center in 1995-2004. ABLL stage I, ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:AIM:to compare respiratory function and laboratory data with the radiographic stages of intrathoracic sarcoidosis. SUBJECTS AND METHODS:Three hundred and eleven patients (70.4% for women and 29.6% for men; mean age, 44.7±0.6 years) with histologically verified sarcoidosis underwent X-ray computed tomography, spirograp...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:10.17116/terarkh201587348-52
更新日期:2015-01-01 00:00:00
abstract:AIM:To specify circulatory disorders in the bed of common carotid artery (CCA) in hypertensive patients and these disorders influence on psychovegetative and hemodynamic disturbances. MATERIAL AND METHODS:Ultrasonic dopplerography of the region of common carotid artery, ECG, examinations of central hemodynamics, veget...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:AIM:To study effects of two hypotensive drugs--normodipin and spirapril--on intravascular activity of platelets in patients with metabolic syndrome (MS). MATERIAL AND METHODS:Normodipin was given to 25 patients with MS for 3 months, spirapril--to 20 patients for 3 months. The effect was evaluated by changes in lipid p...
journal_title:Terapevticheskii arkhiv
pub_type: 临床试验,杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::The atrial complex of ECG transbronchial leads (TB ECG) was in all cases recorded as two waves in line with right and left atrial contractions. Changes in atrial electrical activity reflect alterations in the lesser circulation. A new criterium of TB ECG atrial complex quantitation is suggested devised upon comparison...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::A case of the immunoglobuline deposit disease diagnosis in MM patient having the symptoms of intensive proteinuria, macrohematuria and terminal renal failure is reported. The disease was diagnosed by the evidence from electron microscopy of the kidney and liver biopsies. ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The overall and regional left ventricular ejection fraction (EF) was assessed by rest-exercise equilibrium radionuclide ventriculography in 33 patients (mean age 52.3 +/- 10.3 years) given a single dose of calcium-channel blockers (CCB). Of these, 18 patients were administered 20 mg of nifedipine and 15 patients recei...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::A study was made of the survival time of erythrocytes labeled with 51Cr in 30 patients with hypoplastic anemia. In 96.7% of the patients erythrocyte survival time was reduced (from 18 to 3.6 days; an average of 11.8 days), an increase in the number of macrocytes was noted (in 83.3%). The mean volume and thickness of e...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The trends in atherosclerosis presentation were followed up pathomorphologically in a two-stage epidemiological survey performed in 9 cities of CIC and Baltic states at 25-year interval. The investigations were conducted in accordance with WHO program. Stage 1 (1963-1968) covered 6121 cases, stage 2 (1985-1989) includ...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::A new method of tomography based on nmr (nmr-tomography) was used to examine 25 normal subjects and 35 patients with tumorous, vascular, inflammatory and demyelinization processes occurring in the brain, cervical division of the spinal cord and visceral cranium. Use was made of an nmr-tomograph with a resistive magnet...
journal_title:Terapevticheskii arkhiv
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::The paper summarizes the data of international and Russian studies concerning current approaches to the pharmacotherapy of rheumatoid arthritis. Particular emphasis is placed on the substantiation of the treat-to-target concept, on the efficacy and safety of genetically engineered biological agents, including the inhi...
journal_title:Terapevticheskii arkhiv
pub_type: 历史文章,杂志文章,评审
doi:
更新日期:2013-01-01 00:00:00