Abstract:
:The need to detect and monitor introduced marine species has increased with the increasing number of marine invasions. To complement standard detection and monitoring techniques, new approaches using environmental DNA (eDNA) have recently been developed. However, most of the eDNA work has focused on vertebrate species in spatially limited freshwater habitats while benthic invertebrates in coastal environments have received much less attention. Here, we evaluated the suitability of the eDNA approach for detecting benthic, hard-shelled, crustacean mud crab species in a brackish water environment. We demonstrated for the first time that eDNA from an introduced mud crab Rhithropanopeus harrisii can be successfully amplified in aquarium water samples and detected in the brackish water environment. However, the detection rate was rather low. This suggests that in contrast to freshwater vertebrates, it may be more challenging to develop a highly sensitive eDNA method for detecting crustacean species in a marine environment.
journal_name
Mar Pollut Bulljournal_title
Marine pollution bulletinauthors
Forsström T,Vasemägi Adoi
10.1016/j.marpolbul.2016.05.054subject
Has Abstractpub_date
2016-08-15 00:00:00pages
350-355issue
1eissn
0025-326Xissn
1879-3363pii
S0025-326X(16)30354-Xjournal_volume
109pub_type
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