Abstract:
:We have previously employed cyclization of a linear peptide as a strategy to modulate peptide function and properties, but cleavage to regenerate the linear peptide left parts of the linker structure on the peptide, interfering with its activity. Here, we focused on cyclization of a linear peptide via a "traceless" disulfide-based linkage that would be cleaved and completely removed in a reducing environment, regenerating the original linear peptide without any linker-related structure. Thus, the linker would serve as a redox switch that would be activated in the intracellular environment. We applied this strategy to a lysine-specific demethylase 1 (LSD1) inhibitor peptide 1. The resulting cyclic peptide 2 exhibited approximately 20 times weaker LSD1-inhibitory activity than peptide 1. Upon addition of reducing reagent, the linker was completely removed to regenerate the linear peptide 1, with full restoration of the LSD1-inhibitory activity. In addition, the cyclic peptide was far less susceptible to proteolysis than the linear counterpart. Thus, this switch design not only enables control of functional activity, but also improves stability. This approach should be applicable to a wide range of peptides, and may be useful in the development of peptide pharmaceuticals.
journal_name
Bioorg Med Chemjournal_title
Bioorganic & medicinal chemistryauthors
Amano Y,Umezawa N,Sato S,Watanabe H,Umehara T,Higuchi Tdoi
10.1016/j.bmc.2016.12.033subject
Has Abstractpub_date
2017-02-01 00:00:00pages
1227-1234issue
3eissn
0968-0896issn
1464-3391pii
S0968-0896(16)31208-1journal_volume
25pub_type
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