Abstract:
:The evaluation of the transplanted liver health by non-invasive approaches may offer an improvement in early clinical intervention. As transplanted organs have genomes that are distinct from the host's genome, the quantification of the specific DNA of the donated liver in the patient serum will allow us to obtain information about its damage. We evaluated the state of transplanted liver health by monitoring the RH gene in serum circulating DNA (cirDNA) from 17 recipient and donor mismatched for this gene. cirDNA RH gene was quantified by RT- PCR before, at the moment of transplantation (day 0) and during the stay at the intensive care unit. Beta-globin cirDNA was quantified as a general cellular damage marker. Patients were grouped based on clinical outcomes: (A) patients with no complication; (B) patients that accepted the organ but suffered other complications; (C) patients that suffered organ rejection. All patients showed an increased cirDNA levels at day 0 that decreased until patient stabilization. Patients from groups A and B showed low levels of the RH gene cDNA during the follow-up, with an increase of beta-globin gene at the moment of any clinical complication. Patients from group C showed an increase in the RH gene during rejection.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Macher HC,Suárez-Artacho G,Jiménez-Arriscado P,Álvarez-Gómez S,García-Fernández N,Guerrero JM,Molinero P,Trujillo-Arribas E,Gómez-Bravo MA,Rubio Adoi
10.1007/978-3-319-42044-8_22subject
Has Abstractpub_date
2016-01-01 00:00:00pages
113-116eissn
0065-2598issn
2214-8019journal_volume
924pub_type
杂志文章abstract::Raw soya flour (RSF) feeding to rats produces pancreatic hypertrophy and hyperplasia, and, if sufficiently prolonged, the spontaneous development of pancreatic neoplasms and the potentiation of pancreatic carcinogens. With continuous exposure to RSF the threshold dose for pancreatic growth is approximately 20% RSF, bu...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-0022-0_4
更新日期:1986-01-01 00:00:00
abstract::Alzheimer's disease, a type of dementia, affects memory, behavior, and cognitive processes in affected individuals. It is one of the prominent diseases, accounting for 60-80% of dementia cases and affecting a significant population of persons over the age of 65 years. While rare, Alzheimer's disease (AD) may affect th...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-96445-4_14
更新日期:2018-01-01 00:00:00
abstract::The first systematic multi-center study of the clinical use of EPR oximetry has begun, with funding as a PPG from the NCI. Using particulate oxygen sensitive EPR, materials in three complementary forms (India Ink, "OxyChips", and implantable resonators) the clinical value of the technique will be evaluated. The aims i...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,多中心研究
doi:10.1007/978-3-319-38810-6_13
更新日期:2016-01-01 00:00:00
abstract::(1) Height, weight, total body potassium, exchangeable sodium, bromide space, total body water and concentrations of sodium, potassium and chloride in plasma were measured in control subjects and individuals suffering from alcoholism, with techniques which included body counting and a multiple isotope method using 24N...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4757-0632-1_17
更新日期:1975-01-01 00:00:00
abstract::The analysis of circulating free carnitine and acyl-carnitines provides a powerful selective screening tool for genetic defects in mitochondrial fatty acid oxidation and defects in the catabolism of branched chain amino acids. Using electrospray tandem mass spectrometry (ESI/MS/MS) we developed a sensitive quantitativ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/0-306-46818-2_38
更新日期:1999-01-01 00:00:00
abstract::1. Immunological quantitation of the HPRT proteins, together with DNA and RNA studies have defined further the heterogeneous nature of HPRT-deficiency in our patients. 2. These studies have dictated possible approaches for further characterisation of the HPRT enzyme in our patients. In the two Lesch-Nyhan patients, bo...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-5673-8_24
更新日期:1989-01-01 00:00:00
abstract::THC, the major psychoactive component of marijuana, has been shown both in humans and experimental animals to have immunomodulatory properties. For example, marijuana smokers may show impaired immunological functions, including deficiency of blood leukocyte blastogenesis to mitogens. Detailed studies with mice have sh...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-1951-5_15
更新日期:1995-01-01 00:00:00
abstract::At this point, it may be worthwhile to list, in summary form, the important aspects of divalent cation and nucleotide binding to actin that have been reviewed here: 1) High affinity divalent cation binding to actin is very tight, with equilibrium dissociation constant KCa approximately 1 nM and KMg approximately 5 nM ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-2578-3_4
更新日期:1994-01-01 00:00:00
abstract::Drug resistance is a serious impediment to the treatment of cancer. The use of anti-epidermal growth factor receptor (EGFR) monoclonal antibody therapies in patients with metastatic colorectal cancer is guided by the presence of activating point mutations in KRAS and NRAS genes in the primary tumour. However, RAS wild...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-030-02771-1_9
更新日期:2018-01-01 00:00:00
abstract::In a mouse model of acute light-induced retinal degeneration, positive correlations between the levels of DHA, the levels of n3 PUFA lipid peroxidation, and the vulnerability to photooxidative stress were observed. On the other hand, higher sensitivity of the electroretinogram a-wave response, a measure of the amplifi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4419-1399-9_65
更新日期:2010-01-01 00:00:00
abstract::This paper develops an analysis of the interaction between circulatory convective and tissue diffusive O2 transport that explains how VO2 max is set by the integrated response to each and every step in the O2 transport chain from atmosphere to mitochondria. We suggest that it is not useful to search for "the" rate lim...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-5481-9_22
更新日期:1988-01-01 00:00:00
abstract::The close anatomic juxtaposition of epithelial cells with lymphocytes lining the intestinal tract facilitates communication between the two cell types. This intercellular dialogue is important for mucosal development and has a conditioning effect on mucosal structure, function, and response to tissue injury. Lymphoepi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4615-4143-1_1
更新日期:1999-01-01 00:00:00
abstract::The application of next generation sequencing technologies has opened the door to a new molecular epidemiology of tuberculosis, in which we can now look at transmission at a resolution not possible before. At the same time, new technical and analytical challenges have appeared, and we are still exploring the wider pot...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-64371-7_4
更新日期:2017-01-01 00:00:00
abstract::A solid phase adsorption experiment was performed to detect anti-ETA alpha(NY5 strain ETA) nonneutralizing antibody. Toxin was applied to the Protein A-Sepharose column retaining IgG bound after pretreatment with test serum. Mitogenic activity recovered in the effluent and in the eluate containing the IgG was measured...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4899-1825-3_216
更新日期:1997-01-01 00:00:00
abstract::Cardiovascular disease remains the leading cause of morbidity and mortality for both women and men. Emerging evidence supports that ischemic heart disease (IHD) may manifest differently in women and men, in ways ranging from the clinical presentation, diagnosis, and management of disease to the basic biology and biome...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-77932-4_17
更新日期:2018-01-01 00:00:00
abstract::Inhalation of high concentrations of zinc oxide (ZnO) particles may cause metal fume fever. A useful tool to characterize the reactivity of innate immune cells of an individual, e.g., after in vivo exposure, is the whole blood assay (WBA). The measurable outcome of WBA is the release of cytokines, especially pro-infla...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/5584_2019_471
更新日期:2020-01-01 00:00:00
abstract::The aim of the present study was (i) to investigate the effect of inner speech on cerebral hemodynamics and oxygenation, and (ii) to analyze if these changes could be the result of alternations of the arterial carbon dioxide pressure (PaCO2). To this end, in seven adult volunteers, we measured changes of cerebral abso...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4614-7411-1_12
更新日期:2013-01-01 00:00:00
abstract::The renal alterations induced by alkali-treated protein and lysinoalanine are reviewed and their biological implications discussed. Alkali-treated proteins and lysinoalanine, an unusual amino acid formed in proteins during alkali treatment, have been shown to produce a renal alteration characterized by nuclear and cyt...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4757-9113-6_2
更新日期:1977-01-01 00:00:00
abstract::This book highlights contributions from leaders in the field of sigma receptor research. Sigma receptors represent a promising, novel target for the treatment of neurodegenerative diseases, retinal degenerations, pain and substance abuse. Information is presented about tracers for molecular imaging these receptors, th...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-319-50174-1_1
更新日期:2017-01-01 00:00:00
abstract::For a machine to function, it must first be assembled. The morphogenesis of the simplest icosahedral virus would require only 60 copies of a single capsid protein to coalesce. If the capsid protein's structure could be entirely dedicated to this endeavor, the morphogenetic mechanism would be relatively uncomplicated. ...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4614-0980-9_14
更新日期:2012-01-01 00:00:00
abstract::It is clear that the combination of 31P NMR studies of muscle and of other organs with biochemical and physiological measurements is an extremely powerful method for studying cellular energetics. We have presented examples of our on-going experiments. Well defined and stable in vitro preparations of mammalian fast-twi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-7790-0_27
更新日期:1983-01-01 00:00:00
abstract::The availability of numerical data grows from 1 day to another in a remarkable way. New technologies of high-throughput Next-Generation Sequencing (NGS) are producing DNA sequences. Next-Generation Sequencing describes a DNA sequencing technology which has revolutionized genomic research. In this paper, we perform som...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-030-32622-7_42
更新日期:2020-01-01 00:00:00
abstract::Observations indicate three different tissues of origin for ovarian carcinoma. The ovarian surface epithelium, oviduct epithelium (TSE), and derivates of the Müllerian duct. This chapter discusses the TSE-related ovarian carcinogenesis (exfoliation theory). Recent evidence from prophylactic removed ovaries and Fallopi...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-0-387-68969-2_7
更新日期:2008-01-01 00:00:00
abstract::Antigen 43 (Ag43) is a prominent hetero-oligomeric protein complex in the outer membrane of Escherichia coli. It is composed of two subunits. alpha 43 (M(r) 60, 000) and beta 43 (M(r) 53, 000) in 1:1 stoichiometry. alpha 43 is surface expressed, extends beyond the O-side chains of smooth lipopolysaccharide and is boun...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4899-1828-4_56
更新日期:1997-01-01 00:00:00
abstract::The aim of this study was to establish if patients with hemodynamically relevant or irrelevant stenoses of the extracranial internal carotid artery have different intracranial arterial oxygen saturation as measured by transcranial pulse oximetry using near infrared spectroscopy. Patients with unilateral stenosis > 70%...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-0-387-74911-2_33
更新日期:2008-01-01 00:00:00
abstract::Genotoxic chemicals are known to react with DNA either directly or after metabolic activation to form adducts, a step thought to be relevant with respect to chemical carcinogenesis. Evaluation of cancer risk due to exposure to chemicals requires information about the internal dose which depends on individual variation...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-1-4757-3230-6_20
更新日期:1999-01-01 00:00:00
abstract::There is increasing evidence of close interactions between the adipose and the immune systems. Adipocytes secrete multiple factors, including adipokines such as leptin and adiponectin that have both pro- and anti-inflammatory effects, and influence diseases involving the immune system. Further, adipose tissue also sec...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-0-387-78952-1_1
更新日期:2008-01-01 00:00:00
abstract::We start by describing the functions of the uterus, its structure, both gross and fine, innervation and blood supply. It is interesting to note the diversity of the female's reproductive tract between species and to remember it when working with different animal models. Myocytes are the overwhelming cell type of the u...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-981-13-5895-1_10
更新日期:2019-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) causes abortions and respiratory diseases in pigs. The PRRSV genome is a positive-sense polyadenylated RNA molecule of about 15 kb. Along with the genes for structural proteins (envelope, matrix and nucleocapsid proteins) PRRSV genome contains a number of ORF...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-1899-0_51
更新日期:1995-01-01 00:00:00
abstract::Lysophosphatidic acid (LPA) has major roles as a bioactive signaling molecule, with multiple physiological and pathological roles being described in almost every major organ system. In this review we discuss LPA signaling pathways as emerging drug targets for multiple conditions relevant to human health and disease. L...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-50621-6_7
更新日期:2020-01-01 00:00:00