Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater-treatment plant upgrades.


:The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1223) remained comparable to that downstream of Waterloo (767-3867). Noteworthy was that year and the interaction of year and site explained variability in more than twice the number of transcripts than site alone, suggesting that year and the interaction of year and site had a greater effect on the transcriptome than site alone. Gene set enrichment analysis revealed a gradual reduction in the number of gene ontologies over time at exposure sites, which corresponded with lower contaminant load. Subnetwork enrichment analysis revealed that there were noticeable shifts in the cell pathways differently expressed in the liver preupgrade and postupgrade. The dominant pathways altered preupgrade were related to genetic modifications and cell division, whereas postupgrade they were associated with the immune system, reproduction, and biochemical responses. Molecular pathways were dynamic over time, and following the upgrades, there was little evidence that gene expression profiles in fish collected from high-impact sites postupgrade were more similar to those in fish collected from reference site. Environ Toxicol Chem 2017;36:2108-2122. © 2017 SETAC.


Environ Toxicol Chem


Marjan P,Martyniuk CJ,Fuzzen MLM,MacLatchy DL,McMaster ME,Servos MR




Has Abstract


2017-08-01 00:00:00












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    journal_title:Environmental toxicology and chemistry

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    journal_title:Environmental toxicology and chemistry

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    journal_title:Environmental toxicology and chemistry

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    authors: Monteiro MS,Santos C,Soares AM,Mann RM

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    journal_title:Environmental toxicology and chemistry

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    authors: Maya N,Evans J,Nasuhoglu D,Isazadeh S,Yargeau V,Metcalfe CD

    更新日期:2018-01-01 00:00:00

  • Development and application of a novel method for high-throughput determination of PCDD/Fs and PCBs in sediments.

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    journal_title:Environmental toxicology and chemistry

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    authors: Aguilar L,Williams ES,Brooks BW,Usenko S

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  • Acute and chronic toxicity of glyphosate compounds to glochidia and juveniles of Lampsilis siliquoidea (Unionidae).

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    journal_title:Environmental toxicology and chemistry

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    authors: Bringolf RB,Cope WG,Mosher S,Barnhart MC,Shea D

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    authors: Kurihara R,Shiraishi F,Rajendran RB,Tao H,Horiguchi F,Nakata K,Hashimoto S

    更新日期:2007-12-01 00:00:00

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Schulz R,Liess M

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    authors: Thilagam H,Gopalakrishnan S,Bo J,Wang KJ

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    journal_title:Environmental toxicology and chemistry

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    authors: Regoli F,Winston GW,Gorbi S,Frenzilli G,Nigro M,Corsi I,Focardi S

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    journal_title:Environmental toxicology and chemistry

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    authors: Beiglböck C,Steineck T,Tataruch F,Ruf T

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    authors: Marlatt VL,Hewitt LM,Van Der Kraak G

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    authors: Kelly-Hooper F,Farwell AJ,Pike G,Kennedy J,Wang Z,Grunsky EC,Dixon DG

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审


    authors: Ahrens L,Bundschuh M

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    journal_title:Environmental toxicology and chemistry

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    authors: DeQuattro ZA,Hemming JD,Barry TP

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    journal_title:Environmental toxicology and chemistry

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    authors: Ørsted M,Roslev P

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Osten JR,Soares AM,Guilhermino L

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Forbes VE,Cold A

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Drouillard KG,Fernie KJ,Letcher RJ,Shutt LJ,Whitehead M,Gebink W,Bird DM

    更新日期:2007-02-01 00:00:00

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Pfleeger T,Olszyk D,Lee EH,Plocher M

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Brix KV,Keithly J,DeForest DK,Laughlin J

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Peters A,Lofts S,Merrington G,Brown B,Stubblefield W,Harlow K

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    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章


    authors: Lotufo GR,Farrar JD,Inouye LS,Bridges TS,Ringelberg DB

    更新日期:2001-08-01 00:00:00