Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage.


BACKGROUND:Mesenchymal stem cells (MSCs) may have beneficial effects in reversing intestinal damage resulting from circulatory disorders. The hypothesis of this study is that MSCs increase antioxidant capacity of small bowel tissue following intestinal ischemia reperfusion (I/R) damage. METHODS:A total of 100 rats were used for the control group and three experimental groups, as follows: the sham control, local MSC, and systemic MSC groups. Each group consisted of 10 animals on days 1, 4, and 7 of the experiment. Ischemia was established by clamping the superior mesenteric artery (SMA) for 45min; following this, reperfusion was carried out for 1, 4, and 7days in all groups. In the local and systemic groups, MSCs were administered intravenously and locally just after the ischemia, and they were investigated after 1, 4, and 7days. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) activities, as well as malondialdehyde (MDA) and total protein levels, were measured. Histopathological analysis was performed using light and electron microscopy. The indicators of proliferation from the effects of anti- and pro-inflammatory cytokines were evaluated using immunohistochemistry. RESULTS:MDA was increased (P<0.05) in the sham control group and decreased (P<0.05) in the MSC groups. SOD, CAT, and Gpx were decreased in the local MSC group (P<0.05). The highest level of amelioration was observed on day 7 in the local MSC group via light and electron microscopy. It was found that the MSCs arrived at the damaged intestinal wall in the MSC groups immediately after injection. Pro-inflammatory cytokines interleukin-1β (IL1β), transforming growth factor-β1 (TGFβ1), tumor necrosis factor-α (TNFα), IL6, MIP2, and MPO decreased (P<0.05), while anti-inflammatory cytokines EP3 and IL1ra increased (p<0.05) in the local and systemic MSC groups. In addition, proliferation indicators, such as PCNA and KI67, increased (P<0.05) in the local and systemic MSC groups. CONCLUSIONS:Parallel to our hypothesis, MSC increases the antioxidant capacity of small bowel tissue after intestinal I/R damage. The MSCs migrated to the reperfused small intestine by homing and reduced oxidative stress via the effects of SOD, CAT, and Gpx, as well as reducing the MDA level; thus, they could increase antioxidant capacity of intestine and have a therapeutic effect on the damaged tissue. We think that this effect was achieved via scavenging of oxygen radicals, suppression of pro-inflammatory cytokines, and increasing the expression of anti-inflammatory cytokines.


J Pediatr Surg


Inan M,Bakar E,Cerkezkayabekir A,Sanal F,Ulucam E,Subaşı C,Karaöz E




Has Abstract


2017-07-01 00:00:00














  • Improved quality of life by combined transplantation in Hirschsprung's disease with a very long aganglionic segment.

    abstract:BACKGROUND/PURPOSE:The treatment of children with Hirschsprung's disease beginning in the proximal jejunum remains a challenge for the pediatric surgeon. These patients need a definitive parenteral nutrition, which could lead to a liver impairment. The goal of this work is to assess the quality of life after combined l...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Yann R,Yves A,Dominique J,Thierry Y,Olivier G,Florence L

    更新日期:2003-03-01 00:00:00

  • Alternative method for repair of the difficult infant hernia.

    abstract:BACKGROUND:Inguinal hernia repair in the small infant is often technically difficult. An alternative operative approach has been developed that can simplify troublesome repairs, while decreasing the potential risks of damage to cord structures and of recurrence. METHODS:Thirteen small infants (weight 1400-3000 grams) ...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Applebaum H,Bautista N,Cymerman J

    更新日期:2000-02-01 00:00:00

  • Preventable pediatric trauma deaths in a metropolitan region.

    abstract::All pediatric trauma deaths occurring in metropolitan Toronto (population, 2.2 million) in 1986 were analyzed from the regional coroner's records. Injuries sustained were scored using the Abbreviated Injury Scale (1985; AIS) and Injury Severity Score (ISS). Victims with injuries graded AIS 6 (any region), AIS 5 head/n...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Dykes EH,Spence LJ,Young JG,Bohn DJ,Filler RM,Wesson DE

    更新日期:1989-01-01 00:00:00

  • What pediatric surgeons read: Utilization of APSA e-mail blast literature reviews.

    abstract:PURPOSE:American Pediatric Surgical Association (APSA) committees send monthly email blasts to members with links to peer-reviewed publications. This study assesses the utilization of this service. METHODS:Email-opening and click-through rates were analyzed from 5/2012 to 4/2017 and compared to APSA and industry stand...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章,评审


    authors: Ayub SS,Islam S,Downard CD,Goldin AB,Powell DM,Taylor JA

    更新日期:2019-04-01 00:00:00

  • Appendicitis and malrotation.

    abstract::A 13-year-old child was admitted with abdominal pain suggesting acute appendicitis, but with some atypical findings. A computed tomography scan showed an inflamed appendix and fecalith in the central abdomen, and malrotation. These striking images both established the diagnoses and were useful in planning the operativ...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Vechvitvarakul S,Harris BH

    更新日期:2007-05-01 00:00:00

  • Corticosteroid therapy in biliary atresia.

    abstract::Sixteen patients with biliary atresia had 44 steroid courses for treatment of cholangitis or diminution of bile flow following Kasai hepatic portoenterostomy operations. A "blast" type (high dose/short duration) steroid administration was employed to potentiate the choleretic and anti-inflammatory effect. There was a ...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Karrer FM,Lilly JR

    更新日期:1985-12-01 00:00:00

  • Decreased expression of monocarboxylate transporter 1 and 4 in the branching airway epithelium of nitrofen-induced congenital diaphragmatic hernia.

    abstract:BACKGROUND/PURPOSE:Monocarboxylate transporters (MCTs) are crucial for the maintenance of intracellular pH homeostasis in developing fetal lungs. MCT1/4 is strongly expressed by epithelial airway cells throughout lung branching morphogenesis. Functional inhibition of MCT1/4 in fetal rat lung explants has been shown to ...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Takahashi T,Friedmacher F,Zimmer J,Puri P

    更新日期:2016-06-01 00:00:00

  • Cholangiocyte apoptosis is an early event during induced metamorphosis in the sea lamprey, Petromyzon marinus L.

    abstract:BACKGROUND:Research in biliary atresia has been hindered by lack of a suitable animal model. Lampreys are primitive vertebrates with distinct larval and adult life cycle stages. During metamorphosis the biliary system of the larval lamprey disappears. Lamprey metamorphosis has been proposed as a model for biliary atres...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Boomer LA,Bellister SA,Stephenson LL,Hillyard SD,Khoury JD,Youson JH,Gosche JR

    更新日期:2010-01-01 00:00:00

  • The impact of transfer on pediatric trauma outcomes.

    abstract:BACKGROUND:Recently, concerns have been raised over delays that result from transferring patients to designated trauma centers. This study aimed to assess whether transfer status had an impact on pediatric trauma outcomes. METHODS:Using a local 1996-2014 pediatric trauma database containing 1541 patients, the followin...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Locke T,Rekman J,Brennan M,Nasr A

    更新日期:2016-05-01 00:00:00

  • Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease.

    abstract:BACKGROUND:The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD). METHODS:A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspecte...

    journal_title:Journal of pediatric surgery

    pub_type: 临床试验,杂志文章


    authors: Tran VQ,Lam KT,Truong DQ,Dang MH,Doan TT,Segers V,Butler MW,Robert A,Goyens P,Steyaert H

    更新日期:2016-12-01 00:00:00

  • Malignant peripheral nerve sheath tumor of the vagus nerve in a teenager with the neurofibromatosis 1 gene mutation: a case report.

    abstract::Malignant peripheral nerve sheath tumors account for approximately 5% to 10% of all soft tissue sarcomas in which 25% to 50% are diagnosed in patients with neurofibromatosis 1 (NF1). Tumors are often located in the proximal portion of the upper and lower extremities and trunk, whereas cervical vagus nerve localization...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Sesenna E,Magri AS,Corradi D,Ferri T,Ferri A

    更新日期:2011-08-01 00:00:00

  • Sex-related differences in childhood and adolescent self-inflicted injuries: a National Trauma Databank review.

    abstract:BACKGROUND:Recent reports have demonstrated increasing lethality among young women after inflicted injuries (SII). The aim of this study was to examine sex differences in the methods and outcomes of childhood and adolescent SII. METHODS:The National Trauma Databank (v 7.0) was used to identify all patients 18 years or...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Branco BC,Inaba K,Barmparas G,Talving P,David JS,Plurad D,Green DJ,Demetriades D

    更新日期:2010-04-01 00:00:00

  • Insulin-like growth factor-I protects neuroblastoma against starvation-induced apoptosis and is associated with increased Bcl-2 expression.

    abstract:BACKGROUND/PURPOSE:Aggressive neuroblastomas avoid apoptosis and have increased expression of the antiapoptotic protein, Bcl-2. Insulin-like growth factor-I (IGF-I) is mitogenic and may promote tumor survival by inhibiting apoptosis. The authors hypothesize that IGF-I may protect neuroblastoma cells from apoptosis by u...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Beierle EA,Strande LF,Chen MK

    更新日期:2002-03-01 00:00:00

  • Bilateral choanal atresia in two members of one family.

    abstract::Two children are presented with bilateral choanal atresia: a girl with the typical features of the Treacher Collins syndrome, and a boy, a third cousin of the girl, without mandibulo-facial dysostosis. Simultaneous occurrence of choanal atresia and the Treacher Collins syndrome may be based on a defective ossification...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Moorman-Voestermans K,Vos A

    更新日期:1983-04-01 00:00:00

  • Necrotizing enterocolitis as a prognostic factor for the neurodevelopmental outcome of preterm infants - match control study after 2years.

    abstract:PURPOSE:Necrotizing enterocolitis (NEC) in very low birth weight infants is a risk factor for developmental delay. To our knowledge, there are no studies published investigating the neurodevelopmental outcome of patients with NEC comparing surgically treated and conservatively treated patients versus match paired contr...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Allendorf A,Dewitz R,Weber J,Bakthiar S,Schloesser R,Rolle U

    更新日期:2018-08-01 00:00:00

  • Malignant benign neonatal sacrococcygeal teratoma.

    abstract::Thirty-six patients with benign neonatal sacrococcygeal teratoma (SCT) were treated in our medical center from 1972 to 1990. Mean gestational age was 38.6 +/- 3.3 weeks with a mean birth weight of 3,484.0 +/- 938.5 g. Twenty-nine patients (89%) were females. The majority of the tumors (75%) contained cystic components...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Bilik R,Shandling B,Pope M,Thorner P,Weitzman S,Ein SH

    更新日期:1993-09-01 00:00:00

  • Pure calcium carbonate gallstones in a two year old in association with prenatal calcium supplementation.

    abstract::A two-year-old female presented with cholelithiasis. Preoperative work-up revealed no etiology. Analysis of the stones revealed the content to be pure calcium carbonate. The mother had taken a calcium carbonate supplement during the last four months of the pregnancy. This represents the first report of such an occurre...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Powell RW

    更新日期:1985-04-01 00:00:00

  • Preduodenal portal vein--a cause of intestinal obstruction?

    abstract::A preduodenal position of the portal vein is a rare anomaly reported in 52 cases in the literature. Two additional cases are reported here. In 54% of the cases of preduodenal portal vein (PPV) was combined with high intestinal obstruction and in one half of these cases PPV was considered obstructive. A critical review...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Esscher T

    更新日期:1980-10-01 00:00:00

  • Right congenital diaphragmatic hernia: an 18-year experience.

    abstract:BACKGROUND:Prenatal diagnosis and outcome of right congenital diaphragmatic hernia (RCDH) are far less well known than the more common left CDH (LCDH). In addition, onset of RCDH beyond the neonatal period with a spectrum of atypical symptoms is not unusual. A retrospective analysis of RCDH treated at a single center o...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Midrio P,Gobbi D,Baldo V,Gamba P

    更新日期:2007-03-01 00:00:00

  • An unusual case of monozygotic epigastric heteropagus twinning.

    abstract::Epigastric heteropagus refers to unequal and asymmetric conjoined twins in which the dependent component (parasite) is smaller and usually attached to the epigastrium of the dominant component (autosite). Epigastric heteropagus is a very rare type of conjoined twins. It is generally assumed that conjoined twins repres...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章,评审


    authors: Hwang EH,Han SJ,Lee JS,Lee MK

    更新日期:1996-10-01 00:00:00

  • Early appendectomy vs. conservative management in complicated acute appendicitis in children: A meta-analysis.

    abstract:BACKGROUND:No consensus exists among surgeons regarding the optimal treatment of complicated acute appendicitis in children (CAA). Existing studies present heterogeneity of data and only few studies analyzed free perforated appendicitis (FPA) separately from appendicular abscess (AAb) and appendicular phlegmon (AP). M...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章,meta分析


    authors: Fugazzola P,Coccolini F,Tomasoni M,Stella M,Ansaloni L

    更新日期:2019-11-01 00:00:00

  • Patent ductus arteriosus ligation in neonates: preoperative predictors of poor postoperative outcomes.

    abstract:PURPOSE:The purpose of this study was to identify preoperative predictors of adverse outcomes in infants undergoing surgical ligation of patent ductus arteriosus (PDA). METHODS:Charts of all neonates who underwent PDA ligation at Texas Children's Hospital (Houston, TX) between 2001 and 2006 were retrospectively review...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Naik-Mathuria B,Chang S,Fitch ME,Westhoff J,Brandt ML,Ayres NA,Olutoye OO,Cass DL

    更新日期:2008-06-01 00:00:00

  • The Vacuum Bell device as a sternal lifter: An immediate effect even with a short time use.

    abstract:BACKGROUND:To minimize cardiac perforation during the minimally invasive repair of pectus excavatum (MIRPE), several surgeons have suggested using a suction device to intraoperatively lift the sternum. Whether or not this technique is effective for all PE patients is not yet known. As such, our aim was to quantify the ...

    journal_title:Journal of pediatric surgery

    pub_type: 临床试验,杂志文章


    authors: Togoro SY,Tedde ML,Eisinger RS,Okumura EM,de Campos JRM,Pêgo-Fernandes PM

    更新日期:2018-03-01 00:00:00

  • Delayed neutropenic enterocolitis in a 12-year-old girl treated with total colectomy and J-pouch reservoir.

    abstract::Neutropenic enterocolitis (NE) is a clinicopathologic condition characterized by bowel wall inflammation, which can proceed to necrosis and perforation. It is mostly seen in neutropenic patients with leukemia who undergo induction treatment with chemotherapy. Most often the cecum is involved. The authors present a 12-...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Larsen TK,Qvist N,Bak M

    更新日期:2001-07-01 00:00:00

  • Segmental small bowel allograft--ischemic injury and regeneration.

    abstract:PURPOSE:Small bowel transplantation (SBT) is the ultimate treatment for intestinal failure. It remains unclear as to which intestinal segment is more suitable for use in segmental SBT. The current study aims to assess the susceptibility of various parts of small intestine to ischemia and reperfusion injury and their ca...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Chan KL,Chan KW,Tam PK

    更新日期:1998-11-01 00:00:00

  • The use of perfluorocarbon-associated gas exchange to improve ventilation and decrease mortality after inhalation injury in a neonatal swine model.

    abstract::Patients with tracheobronchial disease frequently require mechanical ventilation during therapy and experience iatrogenic complications such as barotrauma and volutrauma. The purpose of this study was to determine whether perfluorocarbon-associated gas exchange (PAGE) results in lower ventilatory pressures and more ef...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Fitzpatrick JC,Jordan BS,Salman N,Williams J,Cioffi WG Jr,Pruitt BA Jr

    更新日期:1997-02-01 00:00:00

  • The intestinal damage induced by lipid soluble meconium subfraction is profound compared to the intestinal damage induced by water soluble meconium subfraction.

    abstract:BACKGROUND:It is unclear which substances in meconium are responsible for intestinal damage in gastroschisis. An experimental study was designed to investigate the effects of the lipid or water soluble subfractions of meconium on the intestines of gastroschisis in a chick model. METHODS:Meconium was pooled, homogenate...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Ulusoy O,Karakuş OZ,Ateş O,Hakgüder G,Özer E,Olguner M,Akgür FM

    更新日期:2016-08-01 00:00:00

  • Are esophagrams indicated in pediatric patients with spontaneous pneumomediastinum?

    abstract:BACKGROUND/PURPOSE:Spontaneous pneumomediastinum is an infrequent condition in pediatric patients. Presenting symptoms include chest pain, dysphagia, or vomiting, without initiating event. Patients may undergo esophagram because of concern for esophageal perforation as the source for pneumomediastinum, however, abnorma...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Richer EJ,Sanchez R

    更新日期:2016-11-01 00:00:00

  • The current state of evidence-based pediatric surgery.

    abstract:BACKGROUND:The efficiency of medical care in the United States has become intensely scrutinized with expectations from patients, families, payors, lawmakers, and, currently, the President. The most effective vehicle to bring more efficient care is the employment of evidence-based medicine whenever possible. Evidence-ba...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章,评审


    authors: Ostlie DJ,St Peter SD

    更新日期:2010-10-01 00:00:00

  • Long-term total parenteral nutrition-induced hepatobiliary dysfunction in a rabbit model.

    abstract:BACKGROUND/PURPOSE:Currently, the reason for hepatobiliary dysfunction associated with long-term total parenteral nutrition (TPN) is much debated and still unclear. No agreement can be achieved about whether bacteriotoxins and sepsis, enteral starvation, consequences of abdominal operations, or the TPN solution itself ...

    journal_title:Journal of pediatric surgery

    pub_type: 杂志文章


    authors: Loff S,Waag KL,Kränzlin B,Zovko D,Dzakovic A,Jester I,Wirth H,Wessel L

    更新日期:1998-05-01 00:00:00