Toxic and endocrine disrupting effects of wastewater treatment plant influents and effluents on a freshwater isopod Asellus aquaticus (Isopoda, Crustacea).

Abstract:

:In the present study a biological "in vivo" assay, with freshwater isopod Asellus aquaticus, was used to define and evaluate the potential impact of the wastewater treatment plant (WWTP) wastewaters on local wildlife. Samples of both untreated and mechanically and biologically treated WWTP wastewater, were tested in the presence and absence of the formulated sediment for their lethal and sublethal effects. Chronic exposures to wastewater samples caused concentration dependent reduced locomotion, body pigmentation and molting frequency in exposed organisms. The observed effects indicate the overall toxicity and endocrine disruption of the wastewater samples. In contrast stimulations of the feeding rate and growth rate of the test organisms during the chronic exposure to sublethal levels of wastewater samples were observed, indicating an improvement in nutritional quality of the wastewater. The most sensitive exposure endpoint was molting frequency of tested organisms, which indicated the presence of estrogenically active endocrine disrupting compounds (EDCs). Raw wastewater caused up to 42% molting frequency reduction of exposed A. aquaticus when exposed to five times diluted untreated wastewater sample, while undiluted treated wastewater caused a 61% molting frequency reduction. The presence of estrogenically active compounds in the wastewater was confirmed with the yeast estrogen screen assay (YES test), which assigned the highest estrogenic activity to a mechanically and biologically treated wastewater sample, and lower estrogenic activity to all other tested samples. The importance of presence of the formulated sediment was determined, as it lessened the effects of all WWTP wastewater samples in all observed exposures.

journal_name

Chemosphere

journal_title

Chemosphere

authors

Plahuta M,Tišler T,Toman MJ,Pintar A

doi

10.1016/j.chemosphere.2017.01.137

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

342-353

eissn

0045-6535

issn

1879-1298

pii

S0045-6535(17)30159-5

journal_volume

174

pub_type

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