Abstract：
AIM:Breast cancer 2, early onset (BRCA2) has been reported to be associated with familial breast and ovarian cancer. Several proteins interact with conserved regions of BRCA2, which play significant roles in DNA damage repair and centrosomal localization. This study was aimed to identify a novel protein, Wilms tumor 1 interacting protein (WTIP), which might interact with the conserved regions of BRCA2, as well as the functional role of silencing of WTIP in response to centrosomal localization. MATERIALS AND METHODS:Hela S3 cells were used in our study. A yeast two-hybrid screening was used to identify a novel BRCA2-interacting protein. Coimmunoprecipitation and glutathione S-transferase (GST) pull-down assays were performed to detect protein-protein interaction between BRCA2 and hemaglutinin (HA)-WTIP. The expression of WTIP was silenced by short hairpin RNA (shRNA) and the levels of WTIP were confirmed by Western blot. Immunofluorescence microscopy was performed to study the centrosome localization. The functional role of knocking down WTIP expression in response to centrosomal localization was then investigated. RESULTS:The results showed that there was an interaction between WTIP and BRCA2 (amino acids 2750-2864) in Hela S3 cells. We found that WTIP interacted with BRCA2 in both exogenous and endogenous level. The expression levels of WTIP were significantly decreased by siRNA compared to the control group. Downregulation of WTIP abolished BRCA2 centrosome localization and abnormal cell division. CONCLUSION:This study indicates that WTIP interacts with BRCA2 and might be responsible for BRCA2 centrosome localization in cervical cancer cell.
journal_name
Arch Gynecol Obstetjournal_title
Archives of gynecology and obstetricsauthors
Zhang J,Xu J,Wang G,Sun P,Yan T,Zhao Xdoi
10.1007/s00404-016-4176-9subject
Has Abstractpub_date
2016-11-01 00:00:00pages
1311-1316issue
6eissn
0932-0067issn
1432-0711pii
10.1007/s00404-016-4176-9journal_volume
294pub_type
杂志文章abstract：PURPOSE:To revisit risk factors of major obstetric hemorrhage in a large obstetric center. STUDY DESIGN:A retrospective case control study was conducted based on institutional electronic database and blood bank registry of a single center, 2005-2014. The major obstetric hemorrhage event was defined as transfusion of ≥...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-015-3725-y
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:First trimester risk assessment for fetal aneuploidies is computed on the base of a general background risk, which is depending on the maternal age. Thereby, the adjusted risk tends to rise with increasing age. Obversely, more unsuspicious fetal parameters [measurement of the nuchal translucency (NT) and bioc...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-007-0324-6
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND:The last 5 years' studies using next-generation sequencing provided evidences that many types of solid tumors present spatial and temporal genetic heterogeneity and are composed of multiple populations of genetically distinct subclones that evolve over time following a pattern of branched evolution. The evol...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-017-4292-1
更新日期：2017-03-01 00:00:00
abstract：PURPOSE:Laparoscopy has become the standard of care in the management of ovarian benign mucinous cystadenomas. One concern of the operative approach is the risk of recurrence. A previous study has found intraoperative cyst rupture and cystectomy to be the risk factors for recurrence. Our objective was to assess the inc...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-014-3543-7
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to estimate the percentage of patients with metastatic ovarian, fallopian tube, and primary peritoneal cancer requiring ultra-radical surgery to achieve cytoreduction to less than 1 cm (optimal) or no macroscopic residual disease (complete). METHODS:Perioperative data were collected ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-016-4265-9
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study is the evaluation of mechanisms and outcome of nonobstetrics vulvovaginal injuries (VVI). STUDY DESIGN:Medical documentation on 17 VVI recorded during the 1998-2005 period was analysed. The analysis included age, mode of injury infliction (accidental, intracoital, rape), treatment, and ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-006-0228-x
更新日期：2007-02-01 00:00:00
abstract：PURPOSE:The purpose of the study was to investigate the association between gene phosphate and tensin homolog (PTEN) single nucleotide polymorphisms (SNPs) and risk of developing polycystic ovary syndrome (PCOS) in South Indian women. PTEN is one of the most important tumor suppressor genes that regulate cell prolifera...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-020-05658-4
更新日期：2020-10-01 00:00:00
abstract：INTRODUCTION:Objective cutaneous metastasis from carcinoma of the uterine cervix is an uncommon occurrence. The outcome of patients with skin metastasis is usually poor as they are often associated with locoregional recurrence. This metastasis impairs the quality of life and shortens survival. Consequently, physicians ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-008-0617-4
更新日期：2008-11-01 00:00:00
abstract：:Human sperm chromosomes can be visualized after their fusion with zona-free hamster eggs. We analyzed a total of 867 metaphases from 33 men that had been classified into three different groups. We present a detailed summary of the kind and frequency of chromosome aberrations we encountered. A comparison of our results...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：PURPOSE:Chemokines have been implicated in the pathological process of endometriosis. We compared the effects of peritoneal fluid obtained from patients with endometriosis (ePF) and controls without endometriosis (cPF) on the release of monocyte-specific CC chemokines such as monocyte chemotactic protein-1 (MCP-1), reg...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-010-1583-1
更新日期：2011-06-01 00:00:00
abstract：:We studied the prognostic value of anaemia in the evolution of patients with early stages of uterine cervix cancer and treated with radical surgery. An observational study of 114 patients treated for cervical cancer at the "La Fe" Maternity Hospital in Valencia (Spain) during the period 1971 to 1989. Survival analyses...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-002-0296-5
更新日期：2003-01-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to determine the risk factors, prevalance, epidemiological parameters and maternal-perinatal outcome in pregnant women with hypertensive disorder. MATERIALS AND METHODS:A retrospective analysis was undertaken on 255 consecutive cases of hypertensive disorder in pregnancy who were man...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-005-0741-3
更新日期：2005-11-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to investigate the prevalence of human papillomavirus (HPV) genotypes infection in women with and with normal cervical histology in the littoral region of Shandong, China. METHODS:Scrape specimens were collected from 4,601 women for Pap smear and HPV genotypes identification. 1,...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-010-1584-0
更新日期：2011-06-01 00:00:00
abstract：PURPOSE:Color Doppler ultrasound (CDU) accuracy of kidneys and bladder in the diagnosis and treatment of hydronephrosis and/or renal colic in pregnancies was evaluated. METHODS:Between January 2010 and September 2012, 234 pregnant women asymptomatic in 204 cases and with unilateral renal colic in 30 (median 26 years) ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-013-2768-1
更新日期：2013-09-01 00:00:00
abstract：:Ectopic pregnancy is the gynaecological emergency par excellence and remains the leading cause of pregnancy-related first trimester deaths in the UK. Its prevalence continues to rise because of increases in the incidence of the risk factors predisposing to ectopic pregnancy. Classically, the diagnosis is based on a hi...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-008-0731-3
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND:Polymyositis (PM) is an idiopathic inflammatory myopathy. Occasionally, it may present as a paraneoplastic syndrome and it is strongly associated with ovarian and weakly with breast cancer. We present here a case of a 60-year-old patient with sequential breast and ovarian (second primary) carcinomas followed...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-006-0307-z
更新日期：2007-07-01 00:00:00
abstract：INTRODUCTION:Bilateral mucinous cystadenoma of the ovary are extremely rare. These tumors are benign and might lead to abdominal distension, if no secondary symptoms occur and patient delay the consultation of physicians. CASE:A 60-year-old patient was admitted to the internal medicine department for constipation and ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-008-0634-3
更新日期：2009-01-01 00:00:00
abstract：PURPOSE:The present meta-analysis aims to assess the efficacy of cervical cerclage retention after the occurrence of preterm premature rupture of the membranes by analyzing the observed maternal and neonatal perinatal outcomes. METHODS:We searched Medline (1966-2014), Scopus (2004-2014), Clinicaltrials.org (1997-2014)...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-014-3551-7
更新日期：2015-04-01 00:00:00
abstract：METHODS:We investigated cyclin D1 expression in proliferative endometrium, endometrial hyperplasia and endometrioid adenocarcinoma, and examined the correlation of cyclin D1 expression with Ki67 as a cell proliferation associated marker. Immunohistochemical expression of cyclin D1 and Ki67 were studied in 30 cases with...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-003-0595-5
更新日期：2005-02-01 00:00:00
abstract：PURPOSE:Hydrotubation has been considered to be a complimentary procedure to reproductive surgery, usually being conducted after surgery. The objective of this work was to assess the potential value of intra-operative hydrotubation in improving fertility of tubal infertile women. METHODS:180 tubal infertile women were...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00404-012-2525-x
更新日期：2013-01-01 00:00:00
abstract：INTRODUCTION:Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Therefore, host gene...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-007-0504-4
更新日期：2008-06-01 00:00:00
abstract：PURPOSE:To compare the effects of metformin with insulin on maternal and neonatal outcomes in gestational diabetes mellitus (GDM). METHODS:A literature search in PUBMED, EMBASE, Science Direct, Springer link, and Cochrane library was conducted using the following search terms: "Gestational Diabetes" or "GDM", and "ins...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00404-014-3566-0
更新日期：2015-07-01 00:00:00
abstract：:Eighty one invasive breast cancers were analysed immunohistochemically to detect if they expressed the adhesion molecules CD44 v6 and v4/5, and the results were evaluated using the semiquantitative IR-score. The results were further divided into four groups: negative, weak positive, moderate positive and strong positi...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s004040050113
更新日期：1996-01-01 00:00:00
abstract：PURPOSE:To evaluate intracytoplasmic sperm injection (ICSI) outcome of women over age 39 and to determine when to discourage such couples to undergo IVF using their own oocytes. METHODS:Four hundred ninety-five consecutive women (n=668 cycles) over age 39 were evaluated by year-by-year age increments to discriminate t...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-009-1116-y
更新日期：2010-02-01 00:00:00
abstract：:The prevalence of cerebral palsy (CP) has increased over the last 15 years in most countries. This is explained by an improved survival of very low birth weight prematures. In term infants birth asphyxia is of minor significance as a cause for CP. In only 10% of all CP cases following delivery at term, birth asphyxia ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：INTRODUCTION:Acute fatty liver (AFL) of pregnancy is a difficult and challenging diagnosis. The treatment plan is usually supportive. CASE REPORT:We present a case of a patient with AFL of pregnancy and Gram-negative endomyometritis and sepsis. Our treatment plan included the use of activated protein C (APC). CONCLUS...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-004-0716-9
更新日期：2005-12-01 00:00:00
abstract：PURPOSE:To evaluate the therapeutic role of lymphadenectomy on patients with malignant ovarian germ cell tumor (MOGCT) and to investigate the risk factors of lymph node metastasis. METHODS:Patients of MOGCT between 1988 and 2013 with definite lymph node information were extracted from the Surveillance, Epidemiology, a...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-020-05772-3
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Cerebral malformations may lead to permanent postnatal sequels. The antenatal detection of anomalous or absent fetal sulci and gyri may indicate abnormal brain development and future neurological and psychomotor problems in that infant. The prenatal diagnosis of these conditions allows genetic counseling, ps...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-010-1691-y
更新日期：2011-02-01 00:00:00
abstract：:Intrauterine retention of foetal bones is an uncommon but recognised complication of late termination of pregnancy. Secondary subfertility, abnormal uterine bleeding and vaginal discharge are the usual presenting complaints. We report a case of prolonged retention of foetal bones for 14 years in a woman who presented ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-008-0686-4
更新日期：2009-02-01 00:00:00
abstract：PURPOSE:To evaluate the prevalence of cervical pre-malignancies in the cervical cytology of female renal transplant recipients (RTR) and compare to immunocompetent patients. METHODS:A prospective case-control study of 165 RTR (cases) and 372 immunocompetent women (controls) was carried out from May 2015 to August 2016...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-020-05621-3
更新日期：2020-09-01 00:00:00
