Abstract：
OBJECTIVE:We aimed to differentiate dental pulp stem cells (DPSC) to odontoblast-like cells (ODPSC) and to investigate their attachment and growth on dentin in the presence of extra calcium by colorimetric assay and scanning electron microscopy (SEM). METHODS:After isolation of DPSC, they were differentiated to ODPSC. Standard dentin discs from human molar teeth were prepared. While the dentin discs in Group 1 did not receive any extra treatment, the discs in Group 2 were treated with acidic calcium phosphate precipitation (CPP) solution. In Group 3, the discs were suspended in phosphate buffered saline containing calcium. DPSC or ODPSC (3×10(4) cells/mL) were seeded on all discs and incubated for 7, 14 or 21 days. Attachment and growth of 7-day cell cultures on extra dentin samples were examined by SEM. MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p<0.05). RESULTS:While DPSC and ODPSC showed similar proliferation rates at 7 and 14days (p>0.05), the number of ODPSC was higher than DPSC in 21-day samples (p=0.039). MTT assay showed that number of cells on dentin surfaces was increased by time periods regardless of type of treatment and cells (p<0.05). Calcium-treated dentin surfaces always had lower number of cells; being significant for only CPP-treated surfaces (p<0.01). Both types of cells demonstrated good attachment and proliferation on dentin surfaces regardless of type of dentin treatment. CONCLUSIONS:Because the nature of dentin surface itself showed good adhesive characteristics with ODPSC and DPSC, additional calcium treatment of dentin surfaces may not be necessary.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Özdal-Kurt F,Şen BH,Tuğlu I,Vatansever S,Türk BT,Deliloğlu-Gürhan Idoi
10.1016/j.archoralbio.2016.04.008subject
Has Abstractpub_date
2016-08-01 00:00:00pages
131-41eissn
0003-9969issn
1879-1506pii
S0003-9969(16)30109-1journal_volume
68pub_type
杂志文章abstract：:One dentine specimen was prepared from each of 90 bovine incisors. The samples were then evenly distributed among nine groups (A-I) and submitted to 10 alternating de- and re-mineralization cycles, including abrasion by tooth brushing. Each cycle started with a demineralization using the erosive soft drink Sprite Ligh...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00039-5
更新日期：2001-09-01 00:00:00
abstract：OBJECTIVE:To compare the mineralisation density (MD), morphology and histology of alveolar bone and cementum amongst VDR +/+, VDR -/-, and VDR -/- groups supplemented with a diet TD 96348, containing 20% lactose, 2.0% calcium and 1.25% phosphorous. METHODS:Four groups of mice (6 mice/group) were identified by genotypi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.004
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To investigate the effects of mechano-growth factor E (MGF-E) peptide derived from an IGF-1 isoform on the differentiation and mineralization of osteoblasts. METHODS:MGF-E peptide corresponding to the carboxy terminal 24 amino acid peptide of human MGF was synthesized. MGF-E (1 nM) peptide was then used to t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.016
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVES:The antibacterial activity of Coffea canephora extract was evaluated in vitro against Streptococcus mutans and Streptococcus sobrinus. The viability of planktonic cells was analysed by susceptibility tests (MIC and MBC) and time-kill assays. The effect of the extract on dental demineralisation was also inves...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.001
更新日期：2011-06-01 00:00:00
abstract：OBJECTIVE:A loss of functional salivary glands often occurs after radiotherapy for head and neck tumour, and causes many problems in oral health. Recently, the use of salispheres, which consist of salivary gland stem cells (SGSCs), has been suggested as therapy for these problems. However, an insufficient number of cel...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.03.003
更新日期：2015-06-01 00:00:00
abstract：:Histatins 1, 3 and 5 are the major members of a histidine-rich protein family present in human salivary secretions. These proteins are distinct from many salivary proteins in their high positive charge density at neutral pH, and their antibacterial and antifungal properties. In this study, the hydroxyapatite adsorptio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00012-8
更新日期：2003-05-01 00:00:00
abstract：OBJECTIVE:To investigate morphological and biochemical changes in an experimental model of temporomandibular joint (TMJ) arthritis, as well as examine contralateral joint involvement and morphometric aspects of masticatory muscles in affected animals. DESIGN:Forty Wistar rats were allocated into three groups, as follo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.003
更新日期：2018-11-01 00:00:00
abstract：:The pH effects of urea metabolism were studied in washed salivary-sediment bacteria from subjects that had up to 10-fold variation in oral ureolytic activity, and in dispersed artificial-mouth plaques. Adequate evaluation required analysis of the [OH-] as well as the pH curve. An initial constant rate of pH-change, la...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90132-4
更新日期：1987-01-01 00:00:00
abstract：:In culture, chondrocytes of Meckel's cartilage can differentiate further to become bone-type collagen-synthesizing cells. Here, the replacement of type II collagen by type I collagen, accompanying expression of the osteocytic phenotype, was analysed by double immunofluorescence staining, histochemistry and electron mi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00104-0
更新日期：1998-02-01 00:00:00
abstract：:The aim of this study was to compare total IgA in the whole saliva of children with Down syndrome with levels in sibling and parent groups. IgA measurements were presented as the concentration in saliva (μg/ml) and also adjusted for salivary flow rate (SFR; μg/min). Twenty children with Down syndrome, ten siblings and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.03.005
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:The present study was designed to examine how oestrogen regulates proliferation, osteoblastic differentiation, collagen synthesis and periostin gene expression in primary human periodontal ligament (hPDL) cells. DESIGN:The short interfering RNA (siRNA) technique was used to inhibit oestrogen receptor beta (E...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.11.001
更新日期：2011-05-01 00:00:00
abstract：:Dehydration by alcohols and concentrated solutions of highly soluble salts caused refractive changes in enamel which were enhanced in the cervical regions. Electron microscopy showed crystals in cervical enamel to vary from normal orientation, size and habit to marked disorientation and reduced size. Consequent increa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(81)90106-0
更新日期：1981-01-01 00:00:00
abstract：OBJECTIVE:While Aggregatibacter actinomycetemcomitans (Aa) is highly associated with localised aggressive periodontitis (LAP) many Aa-carriers do not develop LAP. This study was designed to determine whether specific salivary factors could distinguish between subjects who have Aa initially and remain healthy (H/AA) as ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.10.009
更新日期：2013-06-01 00:00:00
abstract：:Eight primary incisors obtained from a child with dentinogenesis imperfecta (DI) type II were examined histologically using light microscopy and scanning electron microscopy. In both the DI and control teeth, large dentinal canals were observed along the midline of the crown distributed mesial-distally and coursing to...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90116-5
更新日期：1985-01-01 00:00:00
abstract：:In 14 ovariectomized and 15 control rats, killed at intervals over a 117-day period, the height of the mandible in the molar region was determined radiographically. Mandibular height was significantly related to time after operation in both groups, but their rate of mandibular growth was not significantly different. I...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90110-v
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the factors that could participate on salivary glands hypofunction during inflammation and the participation of endocannabinoids in hyposalivation induced by the presence of inflammogens in the submandibular gland (SMG) or in the brain. DESIGN:Salivary secretion was a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.04.003
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:It was aimed to investigate the in vivo effects of local injection of sclerostin protein on orthodontic tooth movement. DESIGN:A total of 48 rats underwent orthodontic mesialization of the maxillary first molars on both sides. Local injection was given at the compression side in the alveolar bone on both max...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.12.011
更新日期：2019-03-01 00:00:00
abstract：:Analysis of dental microwear may be used to detect species differences in diet and to secure information about occlusal relationships and biomechanics of the jaw. Microscratch-orientation data evaluated with reference to several hypotheses about functional, wear-related, sex and age influences on occlusal contacts ind...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90007-4
更新日期：1984-01-01 00:00:00
abstract：:Tissue transglutaminase (TGase 2) has been implicated in numerous cellular functions, i.e., apoptosis, differentiation, extracellular matrix protein cross-linking and organogenesis. Earlier report of the strong transient expression of TGase 2 localized at the anchoring sites of muscle bundles of human embryo and recen...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.01.007
更新日期：2005-09-01 00:00:00
abstract：:Specific antibodies and indirect immunoperoxidase labelling were used to study the intracellular production of collagen and fibronectin by cells grown on fibronectin-coated glass; the same cell populations seeded on uncoated glass were used as controls. Strong intracellular staining for type I collagen was seen in all...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90089-s
更新日期：1990-01-01 00:00:00
abstract：:In magnetic-stimulation studies where motor-evoked potentials (MEP) are measured on different occasions, accurate relocation of the stimulation site on the scalp is essential. Here a novel method of locating neural stimulation sites was tested and the reproducibility of MEPs in the human masseter assessed. The heads o...
journal_title：Archives of oral biology
pub_type： 临床试验,杂志文章
doi：10.1016/s0003-9969(98)00050-8
更新日期：1998-08-01 00:00:00
abstract：OBJECTIVES:A resin infiltrant was employed for the treatment of active white spot lesions due to its ability to penetrate into the enamel pores and prevent the progression of the lesion. However, limited information is available about its mechanical effect on different artificial enamel lesions as well as on its resist...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.07.011
更新日期：2018-11-01 00:00:00
abstract：:Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00038-q
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVES:Dental evolution affects the geometry of the tooth, but the adaptive relevance of these changes is related to tooth sharpness, complexity, and relief (topography). On a set of laboratory mice, we assessed how wear related to age and food consistency affected molar geometry and topography. DESIGN:Three group...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.028
更新日期：2017-09-01 00:00:00
abstract：OBJECTIVE:The aim of the study was to compare the neonatal line width as well as the composition of the pre-natal and post-natal enamel in deciduous teeth of children with cerebral palsy (CP) to deciduous teeth of healthy children. DESIGN:58 extracted or normally exfoliated deciduous teeth were collected for the study...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：:The biological activity of carboxyl-terminal parathyroid hormone peptide[53-84] (PTH[53-84]) and aminoterminal peptide [1-34] (PTH[1-34]) during cultivation of the lower molar tooth germ from mouse embryos was examined. PTH[1-34] increased alkaline phosphatase activity in the tooth germ at an early developmental stage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00107-6
更新日期：1998-04-01 00:00:00
abstract：:Substituting ferrets for rats and dogs as animal models for craniofacial research is favourable because of the similarity of many of the ferret's anatomical, metabolic and physiological features to those of man. Other advantages are cost-effectiveness and possibly less ethical controversy. However, information on the ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00043-2
更新日期：2002-08-01 00:00:00
abstract：:The transforming growth factor (TGF)-beta superfamily comprises more than 35 structurally related genes that have been implicated in embryonic induction and morphogenesis. Different superfamily members may have distinct regulatory roles in tooth development and maintenance. Degenerate primer sets derived from the high...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(98)00046-6
更新日期：1998-09-01 00:00:00
abstract：:The aim of this study was to test the hypothesis that hypoxia and interleukin-1 (IL-1)beta played a substantial role in gene expressions of matrix metalloproteinases (MMPs) in temporomandibular joint (TMJ) disc cells. The TMJ disc cells were isolated from rabbit TMJ, and cultured in Dulbecco's modified Eagle's medium ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.06.006
更新日期：2005-01-01 00:00:00
abstract：:Enamel and cementum pellicles form by different adsorption of salivary and serum components to the tooth surface. The authors compared the constituents of surface pellicle formed on human enamel and cementum under three conditions: (1) natural pellicle, present on extracted teeth, which was formed by prolonged exposur...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(87)80013-4
更新日期：1987-01-01 00:00:00
