Biological Variation and Reference Change Value Data for Serum Neuron-Specific Enolase in a Turkish Population.

Abstract:

BACKGROUND:Neuron-specific enolase (NSE) is a recognized biomarker for the assessment of cerebral injury in neurological disorders. This study aims to report a definitive assessment of the biological variation (BV) components of this biomarker, including within-subject BV (CVI), between-subject BV (CVG), index of individuality (II), and reference change value (RCV), in a cohort of Turkish participants using an experimental protocol. METHODS:Six blood specimens were collected from each of the 13 apparently healthy volunteers (seven women, six men; ranging in age from 23 to 36) on the same day, every 2 weeks for 2 months. Serum specimens were stored at -20°C until analysis. Neuron-specific enolase levels were evaluated in serum samples using an electrochemiluminescence (ECLIA) immunoassay kit with a Roche Cobas e 411 auto-analyser. ANOVA test was used to calculate the variations. RESULTS:The CVI and CVG for NSE were 21.5% and 28.8%, respectively. Analytical variation (CVA) was calculated as 10.2%. Additionally, II and RCV were calculated as 0.74 and 66% (95% confident interval, CI), respectively. CONCLUSION:As the performance index (PI) was found to be less than 2 (PI = 0.95), it is concluded that the NSE measurements have a desirable performance for analytical imprecision. Since the II was found to be less than 1 (II: 0.74), the reference values will be of little use. Thus, RCV would provide better information for deciding whether a significant change has occurred.

journal_name

J Clin Lab Anal

authors

Matyar S,Goruroglu Ozturk O,Ziyanoglu Karacor E,Yuzbasioglu Ariyurek S,Sahin G,Kibar F,Yaman A,Inal T

doi

10.1002/jcla.21984

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1081-1085

issue

6

eissn

0887-8013

issn

1098-2825

journal_volume

30

pub_type

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