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Detection of NAD(P)H-dependent enzyme activity by time-domain ratiometry of terbium luminescence.

Abstract：

:NAD(P)H-dependent oxidoreductases play important roles in biology. Recently, we reported that the luminescence lifetime of some Tb(3+) complexes is sensitive to NAD(P)H, and we used this phenomenon to detect activities of these enzymes. However, conventional time-resolved luminescence assays are susceptible to static quenchers such as ATP. Herein we describe a detection methodology that overcomes this issue: the intensity of the sample is measured twice with different delay times and the intensity ratio value is used as an index of NAD(P)H concentration. The method is more robust than single-point measurement, and is compatible with high-throughput assays using conventional microplate readers.

journal_name

Bioorg Med Chem Lett

journal_title

Bioorganic & medicinal chemistry letters

authors

Terai T,Ito H,Hanaoka K,Komatsu T,Ueno T,Nagano T,Urano Y

doi

10.1016/j.bmcl.2016.03.038

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

2314-7

issue

9

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(16)30258-X

journal_volume

26

pub_type

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