Abstract:
:Dengue results in substantial human morbidity and significant socio-economic impacts, but a specific dengue therapeutic is not available. The currently available dengue vaccine has low efficacy and high rate of adverse effects, necessitating different strategies for the development of a safer and more efficient vaccine against dengue virus. We describe here a hybrid combination of different-sized gold nanoparticles (AuNPs) and domain III of envelope glycoprotein derived from serotype 2 of dengue virus (EDIII) as dengue subunit vaccine. The efficacy of the EDIII-functionalized AuNPs (AuNP-E) to induce neutralizing antibody in BALB/c mice is evaluated. Obtained results show that AuNP-E induced a high level of antibody which mediates serotype-specific neutralization of dengue virus. More importantly, the level of antibody is dependent on both the size of AuNPs and the concentration of AuNP-E, implicating the possibility to modulate it through adjusting these parameters. These results represent an important step towards the development of tetravalent AuNP-based subunit dengue vaccine. STATEMENT OF SIGNIFICANCE:This research presents a novel subunit vaccine against dengue virus using a hybrid comprising gold nanoparticles (AuNPs) and domain III of envelop protein (EDIII). We proved the neutralizing activity of anti-EDIII antibody induced in immunized mice on Dengue virus serotype 2 in an AuNP core size and concentration dependent manner. The hybrid concept behind this work could also be adopted for the development of a tetravalent vaccine against four serotypes of Dengue virus.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Quach QH,Ang SK,Chu JJ,Kah JCYdoi
10.1016/j.actbio.2018.08.011subject
Has Abstractpub_date
2018-09-15 00:00:00pages
224-235eissn
1742-7061issn
1878-7568pii
S1742-7061(18)30469-0journal_volume
78pub_type
杂志文章abstract::Nitric oxide (NO) is an endogenous molecule with many critical biological functions that depend on its concentration. At high levels, NO provides broad-spectrum antibacterial effects through both its pathogen inhibition and killing abilities. However, its short half-life has been a great challenge to its clinical appl...
journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2008.02.017
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2013.10.031
更新日期:2014-02-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2011.11.001
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2018.04.005
更新日期:2018-05-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2018.03.047
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2007.06.011
更新日期:2008-01-01 00:00:00
abstract::Sr and CO3 co-substituted hydroxyapatite (SrCHA) nanopowder was synthesized by neutralization. The powder was characterized. The improved solubility in Hanks' balanced solution of SrCHA granules (400-600 microm of dimensional range), potentially usable as bone filler, was assessed and compared with that of an analogou...
journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2007.10.010
更新日期:2008-05-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2015.01.042
更新日期:2015-04-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章,评审
doi:10.1016/j.actbio.2018.04.010
更新日期:2018-06-01 00:00:00
abstract::Spherical bioactive glass 13-93 particles, with a particle size distribution of 50-125 microm, were combined with bioabsorbable poly-L,DL-lactide 70/30 using twin-screw extrusion. The composite rods containing 0, 20, 30, 40 and 50 wt% of bioactive glass were further self-reinforced by drawing to a diameter of approxim...
journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2004.11.002
更新日期:2005-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2012.08.024
更新日期:2013-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2006.07.012
更新日期:2007-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2014.08.007
更新日期:2014-11-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2010.08.005
更新日期:2011-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2010.08.017
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2018.06.005
更新日期:2018-07-15 00:00:00
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pub_type: 杂志文章
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更新日期:2016-10-15 00:00:00
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pub_type: 杂志文章
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2017-07-15 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2015.11.036
更新日期:2016-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2017.06.008
更新日期:2017-08-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2017.11.042
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2018.07.041
更新日期:2018-09-15 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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