Transcriptional Approach for Decoding the Mechanism of rpoC Compensatory Mutations for the Fitness Cost in Rifampicin-Resistant Mycobacterium tuberculosis.


:Multidrug-resistant tuberculosis (TB), defined as TB resistant to the two first-line drugs, isoniazid and rifampin, is a serious challenge to global TB eradication efforts. Although mutations in rpoA or rpoC have been proposed to compensate for this fitness cost due to rpoB mutation in rifampicin-resistant Mycobacterium tuberculosis mutants, whether the compensatory effect exists and the underlying mechanisms of compensation remain unclear. Here, we used RNA sequencing to investigate the global transcriptional profiles of 6 rifampin-resistant clinical isolates with either single mutation in rpoB or dual mutations in rpoB/rpoC, as well as 3 rifampin-susceptible clinical isolates, trying to prove the potential compensatory effect of rpoC by transcriptomic alteration. In rifampin-free conditions, rpoC mutation was associated with M. tuberculosis upregulation of ribosomal protein-coding genes, dysregulation of growth-related essential genes and balancing the expression of arginine and glutamate synthesis-associated genes. Upon rifampin exposure of M. tuberculosis isolates, rpoC mutations were associated with the upregulation of the oxidative phosphorylation machinery, which was inhibited in the rpoB single mutants, as well as stabilization of the expression of rifampin-regulated essential genes and balancing the expression of genes involved in metabolism of sulfur-containing amino acids. Taken together, our data suggest that rpoC mutation may compensate for the fitness defect of rifampicin-resistant M. tuberculosis by altering gene expression in response to rifampin exposure.


Front Microbiol


Xu Z,Zhou A,Wu J,Zhou A,Li J,Zhang S,Wu W,Karakousis PC,Yao YF




Has Abstract


2018-11-30 00:00:00








  • Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro.

    abstract::Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. Th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Medina-Alarcón KP,Singulani JL,Voltan AR,Sardi JCO,Petrônio MS,Santos MB,Polaquini CR,Regasini LO,Bolzani VS,da Silva DHS,Chorilli M,Mendes-Giannini MJS,Fusco-Almeida AM

    更新日期:2017-06-12 00:00:00

  • The Soil Microbiome of GLORIA Mountain Summits in the Swiss Alps.

    abstract::While vegetation has intensively been surveyed on mountain summits, limited knowledge exists about the diversity and community structure of soil biota. Here, we study how climatic variables, vegetation, parent material, soil properties, and slope aspect affect the soil microbiome on 10 GLORIA (Global Observation Resea...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Adamczyk M,Hagedorn F,Wipf S,Donhauser J,Vittoz P,Rixen C,Frossard A,Theurillat JP,Frey B

    更新日期:2019-05-15 00:00:00

  • De novo Transcriptome Analysis of Rhizoctonia solani AG1 IA Strain Early Invasion in Zoysia japonica Root.

    abstract::Zoysia japonica brown spot was caused by necrotrophic fungus Rhizoctonia solani invasion, which led to severe financial loss in city lawn and golf ground maintenance. However, little was known about the molecular mechanism of R. solani pathogenicity in Z. japonica. In this study we examined early stage interaction bet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zhu C,Ai L,Wang L,Yin P,Liu C,Li S,Zeng H

    更新日期:2016-05-18 00:00:00

  • Oral Treatments With Probiotics and Live Salmonella Vaccine Induce Unique Changes in Gut Neurochemicals and Microbiome in Chickens.

    abstract::Cross-talk between the gut microbiota and neurochemicals affects health and well-being of animals. However, little is known about this interaction in chickens despite their importance in food production. Probiotics and live Salmonella vaccines are microbial products commonly given orally to layer pullets to improve he...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Redweik GAJ,Daniels K,Severin AJ,Lyte M,Mellata M

    更新日期:2020-01-15 00:00:00

  • Engineering nanoparticles to silence bacterial communication.

    abstract::The alarming spread of bacterial resistance to traditional antibiotics has warranted the study of alternative antimicrobial agents. Quorum sensing (QS) is a chemical cell-to-cell communication mechanism utilized by bacteria to coordinate group behaviors and establish infections. QS is integral to bacterial survival, a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Miller KP,Wang L,Chen YP,Pellechia PJ,Benicewicz BC,Decho AW

    更新日期:2015-03-10 00:00:00

  • Cholecystectomy Damages Aging-Associated Intestinal Microbiota Construction.

    abstract::The intestinal microbiome is essential in humans to maintain physiological balance and nutrition metabolism. Laparoscopic cholecystectomy due to gallstone disease and cholecystitis can cause intestinal microbial dysbiosis, and following bile acid metabolism dysfunction, positions the patient at high risk of colorectal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Wang W,Wang J,Li J,Yan P,Jin Y,Zhang R,Yue W,Guo Q,Geng J

    更新日期:2018-06-25 00:00:00

  • Gastric Damage and Cancer-Associated Biomarkers in Helicobacter pylori-Infected Children.

    abstract::Helicobacter pylori (H. pylori) is well-known to be involved in gastric carcinogenesis, associated with deregulation of cell proliferation and epigenetic changes in cancer-related genes. H. pylori infection is largely acquired during childhood, persisting long-term in about half of infected individuals, a subset of wh...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: George S,Lucero Y,Torres JP,Lagomarcino AJ,O'Ryan M

    更新日期:2020-02-12 00:00:00

  • Molecular Mechanisms of Colistin Resistance in Klebsiella pneumoniae Causing Bacteremia from India-A First Report.

    abstract::Colistin has long been a reserve drug used for the treatment of carbapenem resistant Klebsiella pneumoniae. Carbapenem resistance in K. pneumoniae has been increasing and is as high as 44% in India. Although a reserve agent, with rise in rates of resistance to carbapenems, the usage of colistin has increased over the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Pragasam AK,Shankar C,Veeraraghavan B,Biswas I,Nabarro LE,Inbanathan FY,George B,Verghese S

    更新日期:2017-01-09 00:00:00

  • The Naphthalene Catabolic Genes of Pseudomonas putida BS3701: Additional Regulatory Control.

    abstract::Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence of macro- and micronutrients. Widely varying concentrations of available nitrogen and iron (Fe) in soils were shown to affect residual hydroc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Pozdnyakova-Filatova I,Petrikov K,Vetrova A,Frolova A,Streletskii R,Zakharova M

    更新日期:2020-06-05 00:00:00

  • An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes.

    abstract::Microbial communities in the endosphere of Salicaceae plants, poplar (Populus trichocarpa) and willow (Salix sitchensis), have been demonstrated to be important for plant growth promotion, protection from biotic and abiotic stresses, and degradation of toxic compounds. Our study aimed to investigate bio-control activi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Kandel SL,Firrincieli A,Joubert PM,Okubara PA,Leston ND,McGeorge KM,Mugnozza GS,Harfouche A,Kim SH,Doty SL

    更新日期:2017-03-13 00:00:00

  • Interactions between Obesity Status and Dietary Intake of Monounsaturated and Polyunsaturated Oils on Human Gut Microbiome Profiles in the Canola Oil Multicenter Intervention Trial (COMIT).

    abstract::Long-term dietary fatty acid intake is believed to induce changes in the human gut microbiome which might be associated with human health or obesity status; however, considerable debate remains regarding the most favorable ratios of fatty acids to optimize these processes. The objective of this sub-study of a double-b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Pu S,Khazanehei H,Jones PJ,Khafipour E

    更新日期:2016-10-10 00:00:00

  • GABA-Producing Natural Dairy Isolate From Artisanal Zlatar Cheese Attenuates Gut Inflammation and Strengthens Gut Epithelial Barrier in vitro.

    abstract::Probiotic bacteria are recognized for their health-promoting properties, including maintenance of gut epithelial integrity and host immune system homeostasis. Taking into account the beneficial health-promoting effects of GABA, the presence of the gadB gene, encoding glutamate decarboxylase that converts L-glutamate t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Sokovic Bajic S,Djokic J,Dinic M,Veljovic K,Golic N,Mihajlovic S,Tolinacki M

    更新日期:2019-03-18 00:00:00

  • Genome Sequence, Assembly, and Characterization of the Antagonistic Yeast Candida oleophila Used as a Biocontrol Agent Against Post-harvest Diseases.

    abstract::Candida oleophila is an effective biocontrol agent used to control post-harvest diseases of fruits and vegetables. C. oleophila I-182 was the active agent used in the first-generation yeast-based commercial product, Aspire®, for post-harvest disease management. Several action modes, like competition for nutrients and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Sui Y,Wisniewski M,Droby S,Piombo E,Wu X,Yue J

    更新日期:2020-02-25 00:00:00

  • Temporal Bacterial Surveillance of Salmon Aquaculture Sites Indicates a Long Lasting Benthic Impact With Minimal Recovery.

    abstract::Coastal aquaculture has experienced substantial growth in the last few decades and associated impacts on natural environments are of increasing importance. Understanding both the effects of aquaculture on marine ecosystems and the processes of recovery during fallowing periods is crucial for the development of a more ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Verhoeven JTP,Salvo F,Knight R,Hamoutene D,Dufour SC

    更新日期:2018-12-12 00:00:00

  • Pedunculate Oaks (Quercus robur L.) Differing in Vitality as Reservoirs for Fungal Biodiversity.

    abstract::Ecological significance of trees growing in urban and peri-urban settings is likely to increase in future land-use regimes, calling for better understanding of their role as potential reservoirs or stepping stones for associated biodiversity. We studied the diversity of fungal endophytes in woody tissues of asymptomat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Agostinelli M,Cleary M,Martín JA,Albrectsen BR,Witzell J

    更新日期:2018-08-03 00:00:00

  • Depth Influences Symbiodiniaceae Associations Among Montastraea cavernosa Corals on the Belize Barrier Reef.

    abstract::In Belize, shallow populations (10 and 16 m) of the coral species Montastraea cavernosa from the back reef and reef crest are genetically differentiated from deeper populations on the fore reef and reef wall (25 and 35 m). Like many species of scleractinian corals, M. cavernosa has an obligate symbiosis with dinoflage...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Eckert RJ,Reaume AM,Sturm AB,Studivan MS,Voss JD

    更新日期:2020-04-09 00:00:00

  • Porcine Circovirus-Like Virus P1 Inhibits Wnt Signaling Pathway in Vivo and in Vitro.

    abstract::Porcine circovirus-like virus P1 is an important pathogen of the current pig industry, the infection mechanism is not entirely clear. Wnt signaling pathway plays an important role in the growth of young animals and infection of some viruses. This study was designed to demonstrate the effects of P1 infection on the Wnt...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zhu X,Wen L,Sheng S,Wang W,Xiao Q,Qu M,Hu Y,Liu C,He K

    更新日期:2018-03-12 00:00:00

  • Exploring the Genomic Traits of Non-toxigenic Vibrio parahaemolyticus Strains Isolated in Southern Chile.

    abstract::Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after the rise and fall of the pandemic strain in Chile, other post-pandemic strains have been associated with clinical cases, including strains lacking the major toxins TDH and TRH. Since the prese...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Castillo D,Pérez-Reytor D,Plaza N,Ramírez-Araya S,Blondel CJ,Corsini G,Bastías R,Loyola DE,Jaña V,Pavez L,García K

    更新日期:2018-02-08 00:00:00

  • Marek's Disease Virus (Gallid alphaherpesvirus 2)-Encoded miR-M2-5p Simultaneously Promotes Cell Proliferation and Suppresses Apoptosis Through RBM24 and MYOD1-Mediated Signaling Pathways.

    abstract::MicroRNAs (miRNAs) have been demonstrated for their involvement in virus biology and pathogenesis, including functioning as key determinants of virally-induced cancers. As an important oncogenic α-herpesvirus affecting poultry health, Marek's disease virus serotype 1 [Gallid alphaherpesvirus 2 (GaHV-2)] induces rapid-...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zhu ZJ,Teng M,Li HZ,Zheng LP,Liu JL,Chai SJ,Yao YX,Nair V,Zhang GP,Luo J

    更新日期:2020-11-03 00:00:00

  • Genomic Signatures for Sedimentary Microbial Utilization of Phytoplankton Detritus in a Fast-Flowing Estuary.

    abstract::In fast-flowing, river-dominated estuaries, "hotspots" of microbial biogeochemical cycling can be found within areas of extended water retention. Lateral bays located off of the North and South channels of the Columbia River estuary are proposed to be such hotspots. Previous metagenomic studies on water samples indica...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Smith MW,Herfort L,Rivers AR,Simon HM

    更新日期:2019-11-05 00:00:00

  • Conversion of Methionine to Cysteine in Lactobacillus paracasei Depends on the Highly Mobile cysK-ctl-cysE Gene Cluster.

    abstract::Milk and dairy products are rich in nutrients and are therefore habitats for various microbiomes. However, the composition of nutrients can be quite diverse, in particular among the sulfur containing amino acids. In milk, methionine is present in a 25-fold higher abundance than cysteine. Interestingly, a fraction of s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Wüthrich D,Irmler S,Berthoud H,Guggenbühl B,Eugster E,Bruggmann R

    更新日期:2018-10-17 00:00:00

  • Experimental Evolution on a Wild Mammal Species Results in Modifications of Gut Microbial Communities.

    abstract::Comparative studies have shown that diet, life history, and phylogeny interact to determine microbial community structure across mammalian hosts. However, these studies are often confounded by numerous factors. Selection experiments offer unique opportunities to validate conclusions and test hypotheses generated by co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Kohl KD,Sadowska ET,Rudolf AM,Dearing MD,Koteja P

    更新日期:2016-05-04 00:00:00

  • Vertical Beta-Diversity of Bacterial Communities Depending on Water Stratification.

    abstract::Many studies indicate that variation of marine bacterial beta diversity in the horizontal dimension is mainly attributable to environmental and spatial effects. However, whether and how these two effects drive bacterial beta diversity in the vertical dimension remains unclear, especially when considering seasonal vari...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Cheng WH,Lu HP,Chen CC,Jan S,Hsieh CH

    更新日期:2020-03-19 00:00:00

  • Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range.

    abstract::The survival of Shewanella oneidensis MR-1 at up to 1500 MPa was investigated by laboratory studies involving exposure to high pressure followed by evaluation of survivors as the number (N) of colony forming units (CFU) that could be cultured following recovery to ambient conditions. Exposing the wild type (WT) bacter...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Hazael R,Foglia F,Kardzhaliyska L,Daniel I,Meersman F,McMillan P

    更新日期:2014-11-17 00:00:00

  • Adaptation to Bleaching: Are Thermotolerant Symbiodiniaceae Strains More Successful Than Other Strains Under Elevated Temperatures in a Model Symbiotic Cnidarian?

    abstract::The ability of some symbiotic cnidarians to resist and better withstand stress factors that cause bleaching is a trait that is receiving increased attention. The adaptive bleaching hypothesis postulates that cnidarians that can form a stable symbiosis with thermotolerant Symbiodiniaceae strains may cope better with in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Newkirk CR,Frazer TK,Martindale MQ,Schnitzler CE

    更新日期:2020-05-05 00:00:00

  • The Lactococcus lactis Pan-Plasmidome.

    abstract::Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Kelleher P,Mahony J,Bottacini F,Lugli GA,Ventura M,van Sinderen D

    更新日期:2019-04-04 00:00:00

  • Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce Long-Term Adaptation of E. coli Cultures in Large-Scale Bioreactors: Experimental Evidence and Mathematical Model.

    abstract::Rapidly changing concentrations of substrates frequently occur during large-scale microbial cultivations. These changing conditions, caused by large mixing times, result in a heterogeneous population distribution. Here, we present a powerful and efficient modeling approach to predict the influence of varying substrate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Nieß A,Löffler M,Simen JD,Takors R

    更新日期:2017-06-28 00:00:00

  • Pathobiomes Differ between Two Diseases Affecting Reef Building Coralline Algae.

    abstract::Crustose coralline algae (CCA) are major benthic calcifiers that play crucial roles in coral reef ecosystems. Two diseases affecting CCA have recently been investigated: coralline white band syndrome (CWBS) and coralline white patch disease (CWPD). These diseases can trigger major losses in CCA cover on tropical coral...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Meistertzheim AL,Nugues MM,Quéré G,Galand PE

    更新日期:2017-09-01 00:00:00

  • Diversity and Composition of Airborne Fungal Community Associated with Particulate Matters in Beijing during Haze and Non-haze Days.

    abstract::To assess the diversity and composition of airborne fungi associated with particulate matters (PMs) in Beijing, China, a total of 81 PM samples were collected, which were derived from PM2.5, PM10 fractions, and total suspended particles during haze and non-haze days. The airborne fungal community in these samples was ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Yan D,Zhang T,Su J,Zhao LL,Wang H,Fang XM,Zhang YQ,Liu HY,Yu LY

    更新日期:2016-04-14 00:00:00

  • Chitosan, a Biopolymer With Triple Action on Postharvest Decay of Fruit and Vegetables: Eliciting, Antimicrobial and Film-Forming Properties.

    abstract::Chitosan is a natural biopolymer from crab shells that is known for its biocompatibility, biodegradability, and bioactivity. In human medicine, chitosan is used as a stabilizer for active ingredients in tablets, and is popular in slimming diets. Due to its low toxicity, it was the first basic substance approved by the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Romanazzi G,Feliziani E,Sivakumar D

    更新日期:2018-12-04 00:00:00