Abstract:
:An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase encoded by bacteriophage T7 is naturally endowed with or can be engineered to have all these characteristics. The chemically or genetically modified enzyme (Sequenase) expedited significantly the development of DNA sequencing technology. This article reviews the history of studies on T7 DNA polymerase with emphasis on the serial key steps leading to its use in DNA sequencing. Lessons from the study and development of T7 DNA polymerase have and will continue to enlighten the characterization of novel DNA polymerases from newly discovered microbes and their modification for use in biotechnology.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Zhu Bdoi
10.3389/fmicb.2014.00181subject
Has Abstractpub_date
2014-04-16 00:00:00pages
181issn
1664-302Xjournal_volume
5pub_type
杂志文章,评审abstract::Pestiviruses are highly variable RNA viruses. A growing number of novel pestiviruses has been discovered in domestic and wild species in the last two decades. Recently, a novel atypical porcine pestivirus (APPV) linked with the development of congenital tremor (CT) in neonatal pigs was described in Europe and the Amer...
journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2017.00862
更新日期:2017-05-11 00:00:00
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pub_type: 杂志文章,评审
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abstract::Aquilaria malaccensis Lam, commonly known as Agarwood, is a highly valuable species used in production of agar oil from its infected wood, which is utilized in pharmaceutical and perfumery industry. Agar oil formation in agarwood takes years through the natural process which is induced by natural or artificial injury ...
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abstract::QepA is one of the genes that confer quinolone resistance in bacteria. The aim of this study was to analyze the genetic structures of plasmids that carry a qepA3, a recently discovered allele of qepA in Enterobacteriaceae clinical isolates. 656 non-redundant Enterobacteriaceae clinical isolates were screened for the q...
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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abstract::Aflatoxins are highly toxic carcinogens that detrimentally influence profitability of agriculture and the health of humans and domestic animals. Several phylogenetically distinct fungi within Aspergillus section Flavi have S-morphology (average sclerotial size < 400 μm), and consistently produce high concentrations of...
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2019-05-22 00:00:00
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journal_title:Frontiers in microbiology
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更新日期:2020-01-22 00:00:00
abstract::Syngas generated by thermal gasification of biomass or coal can be steam reformed and purified into methane, which could be used locally for energy needs, or re-injected in the natural gas grid. As an alternative to chemical catalysis, the main components of the syngas (CO, CO2, and H2) can be used as substrates by a ...
journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2016.01188
更新日期:2016-08-03 00:00:00
abstract::During a regular monitoring of antimicrobial resistance in a farrowing farm in Southern China, 117 Escherichia coli isolates were obtained from sows and piglets. Compared with the isolates from piglets, the isolates from sows exhibited higher resistance rates to the tested cephalosporins. Correspondingly, the total de...
journal_title:Frontiers in microbiology
pub_type: 杂志文章
doi:10.3389/fmicb.2015.01210
更新日期:2015-11-03 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章,评审
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2016-11-11 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2017-10-06 00:00:00
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journal_title:Frontiers in microbiology
pub_type: 杂志文章
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更新日期:2011-11-17 00:00:00
abstract::[This corrects the article on p. 42 in vol. 9, PMID: 29459850.]. ...
journal_title:Frontiers in microbiology
pub_type: 已发布勘误
doi:10.3389/fmicb.2018.00995
更新日期:2018-06-11 00:00:00