Impact of Sample Preservation and Manipulation on Insect Gut Microbiome Profiling. A Test Case With Fruit Flies (Diptera, Tephritidae).

Abstract:

:High-throughput sequencing (HTS) techniques are of great value for the investigation of microbial communities, and have been extensively used to study the gut microbiome. While most studies focus on the human gut, many others have investigated insects. However, because of the rapid spread of HTS techniques, a lot of variation exists in the protocols for sample preparation. In the present study, we investigated the impact of two widely adopted sample-processing procedures preceding library preparation, i.e., preservation of insect tissue in 70% ethanol (EtOH) and sample dissection. We used the fruit fly Ceratitis capitata (Diptera: Tephritidae) as a model organism and set up two experiments, one comparing the effects of sample manipulation and preservation across life stages and the other across fruit samples from different sources. The results of this study showed no major effects of dissection on the outcome of HTS. However, EtOH preservation did have effects on the recovered gut microbiome, the main effect being a significant reduction of the dominant genus, Providencia, in EtOH-preserved samples. Less abundant bacterial groups were also affected resulting in altered microbial profiles obtained from samples preserved in 70% EtOH. These results have important implications for the planning of future studies and when comparing studies that used different sample preparation protocols.

journal_name

Front Microbiol

authors

De Cock M,Virgilio M,Vandamme P,Augustinos A,Bourtzis K,Willems A,De Meyer M

doi

10.3389/fmicb.2019.02833

subject

Has Abstract

pub_date

2019-12-13 00:00:00

pages

2833

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Colonization of Mice With Amoxicillin-Associated Klebsiella variicola Drives Inflammation via Th1 Induction and Treg Inhibition.

    abstract::β-Lactam antibiotics can increase the resistance and virulence of individual intestinal microorganisms, which may affect host physiology and health. Klebsiella, a crucial gut inhabitant, has been confirmed to be resistant to most β-lactam antibiotics and contributes to the etiology of inflammatory bowel disease (IBD)....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01256

    authors: Lin H,Wang Q,Liu L,Chen Z,Das R,Zhao Y,Mao D,Luo Y

    更新日期:2020-06-24 00:00:00

  • Reveromycins A and B from Streptomyces sp. 3-10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System.

    abstract::This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00550

    authors: Lyu A,Liu H,Che H,Yang L,Zhang J,Wu M,Chen W,Li G

    更新日期:2017-04-03 00:00:00

  • Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: expanding the mycobacterial phosphoproteome catalog.

    abstract::Reversible protein phosphorylation, regulated by protein kinases and phosphatases, mediates a switch between protein activity and cellular pathways that contribute to a large number of cellular processes. The Mycobacterium tuberculosis genome encodes 11 Serine/Threonine kinases (STPKs) which show close homology to euk...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00006

    authors: Fortuin S,Tomazella GG,Nagaraj N,Sampson SL,Gey van Pittius NC,Soares NC,Wiker HG,de Souza GA,Warren RM

    更新日期:2015-02-10 00:00:00

  • Low Affinity DnaA-ATP Recognition Sites in E. coli oriC Make Non-equivalent and Growth Rate-Dependent Contributions to the Regulated Timing of Chromosome Replication.

    abstract::Although the mechanisms that precisely time initiation of chromosome replication in bacteria remain unclear, most clock models are based on accumulation of the active initiator protein, DnaA-ATP. During each cell division cycle, sufficient DnaA-ATP must become available to interact with a distinct set of low affinity ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01673

    authors: Rao P,Rozgaja TA,Alqahtani A,Grimwade JE,Leonard AC

    更新日期:2018-07-26 00:00:00

  • 5-Aminosalicylic Acid Alters the Gut Bacterial Microbiota in Patients With Ulcerative Colitis.

    abstract::Background: The aim of this study was to clarify the effect of 5-aminosalicylic acid (5-ASA) treatment on gut bacterial microbiota in patients with ulcerative colitis (UC). Methods: A total of 57 UC patients, including 20 untreated and 37 5-ASA-treated, were recruited into an exploration cohort. We endoscopically coll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01274

    authors: Xu J,Chen N,Wu Z,Song Y,Zhang Y,Wu N,Zhang F,Ren X,Liu Y

    更新日期:2018-06-13 00:00:00

  • The Inhibitory Activities and Antiviral Mechanism of Medicinal Plant Ingredient Quercetin Against Grouper Iridovirus Infection.

    abstract::Singapore grouper iridovirus (SGIV) causes high mortality rates in mariculture, and effective treatments against SGIV infection are urgently required. Illicium verum Hook. f. (I. verum) is a well-known medicinal plant with a variety of biological activities. The natural ingredient quercetin isolated from I. verum coul...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.586331

    authors: Liu M,Yu Q,Xiao H,Li M,Huang Y,Zhang Q,Li P

    更新日期:2020-10-16 00:00:00

  • Cross-Talk between the Aeromonas hydrophila Type III Secretion System and Lateral Flagella System.

    abstract::Aeromonas hydrophila is responsible for aeromonad septicaemia in fish, and gastroenteritis and wound infections in humans. The type III secretion system (T3SS) is utilized by aeromonads to inject protein effectors directly into host cells. One of the major genetic regulators of the T3SS in several bacterial species is...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01434

    authors: Zhao YH,Shaw JG

    更新日期:2016-09-07 00:00:00

  • Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning.

    abstract::The Staphylococcus aureus enterotoxins are a superfamily of secreted virulence factors that share structural and functional similarities and possess potent superantigenic activity causing disruptions in adaptive immunity. The enterotoxins can be separated into two groups; the classical (SEA-SEE) and the newer (SEG-SEl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00436

    authors: Fisher EL,Otto M,Cheung GYC

    更新日期:2018-03-13 00:00:00

  • Characterization of the Culturable Subpopulations of Lactobacillus in the Chicken Intestinal Tract as a Resource for Probiotic Development.

    abstract::To gain better understanding of the distributions of the culturable Lactobacillus species in the chicken intestinal tract, we collected ceca, and distal ileum from 10 3-weeks-old broiler chickens. Lactobacillus strains from cecal lumen contents (M-CL), and those associated with mucosa of ceca (M-CM) and ileum (M-IM) w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01389

    authors: Adhikari B,Kwon YM

    更新日期:2017-07-26 00:00:00

  • GPD1 and ADH3 Natural Variants Underlie Glycerol Yield Differences in Wine Fermentation.

    abstract::Glycerol is one of the most important by-products of alcohol fermentation, and depending on its concentration it can contribute to wine flavor intensity and aroma volatility. Here, we evaluated the potential of utilizing the natural genetic variation of non-coding regions in budding yeast to identify allelic variants ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01460

    authors: Tapia SM,Cuevas M,Abarca V,Delgado V,Rojas V,García V,Brice C,Martínez C,Salinas F,Larrondo LF,Cubillos FA

    更新日期:2018-07-03 00:00:00

  • Biophysical methods for the study of microbial surfaces.

    abstract::The challenge in studying the surface architecture of different microbial pathogens is to integrate the most current biochemical, spectroscopic, microscopic, and processing techniques. Individually these methods have insufficient sensitivity to reveal complex structures, such as branched, large, viscous polymers with ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00207

    authors: Frases S,Viana NB,Casadevall A

    更新日期:2011-10-10 00:00:00

  • A Trypsin Inhibitor from Tecoma stans Leaves Inhibits Growth and Promotes ATP Depletion and Lipid Peroxidation in Candida albicans and Candida krusei.

    abstract::Tecoma stans (yellow elder) has shown medicinal properties and antimicrobial activity. Previous reports on antifungal activity of T. stans preparations and presence of trypsin inhibitor activity from T. stans leaves stimulated the investigation reported here. In this work, we proceeded to the purification and characte...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00611

    authors: Patriota LL,Procópio TF,de Souza MF,de Oliveira AP,Carvalho LV,Pitta MG,Rego MJ,Paiva PM,Pontual EV,Napoleão TH

    更新日期:2016-04-27 00:00:00

  • Diversity and Composition of Airborne Fungal Community Associated with Particulate Matters in Beijing during Haze and Non-haze Days.

    abstract::To assess the diversity and composition of airborne fungi associated with particulate matters (PMs) in Beijing, China, a total of 81 PM samples were collected, which were derived from PM2.5, PM10 fractions, and total suspended particles during haze and non-haze days. The airborne fungal community in these samples was ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00487

    authors: Yan D,Zhang T,Su J,Zhao LL,Wang H,Fang XM,Zhang YQ,Liu HY,Yu LY

    更新日期:2016-04-14 00:00:00

  • Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants.

    abstract::RNA silencing, an evolutionarily conserved and sequence-specific gene-inactivation system, has a pivotal role in antiviral defense in most eukaryotic organisms. In plants, a class of exogenous small RNAs (sRNAs) originating from the infecting virus called virus-derived small interfering RNAs (vsiRNAs) are predominantl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.01237

    authors: Zhang C,Wu Z,Li Y,Wu J

    更新日期:2015-11-09 00:00:00

  • Untangling the fungal niche: the trait-based approach.

    abstract::Fungi are prominent components of most terrestrial ecosystems, both in terms of biomass and ecosystem functioning, but the hyper-diverse nature of most communities has obscured the search for unifying principles governing community organization. In particular, unlike plants and animals, observational studies provide l...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00579

    authors: Crowther TW,Maynard DS,Crowther TR,Peccia J,Smith JR,Bradford MA

    更新日期:2014-10-31 00:00:00

  • Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.

    abstract::Bacillus cereus is a Gram-positive pathogen that causes a wide variety of diseases in humans. It secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. EntD is a novel exoprotein identified by proteogenomics of B. cereus ATCC 14579. We constructed a ΔentD mutant an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01004

    authors: Omer H,Alpha-Bazin B,Brunet JL,Armengaud J,Duport C

    更新日期:2015-10-07 00:00:00

  • Molecular Detection of Azole-Resistant Aspergillus fumigatus in Clinical Samples.

    abstract::Aspergillus diseases are often caused by Aspergillus fumigatus. Azoles are the mainstay of therapy, but the management of aspergillosis is hampered by the emergence of azole resistance. Rapid detection of azole resistance might benefit treatment outcome by early treatment modifications. However, the yield of fungal cu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00515

    authors: Buil JB,Zoll J,Verweij PE,Melchers WJG

    更新日期:2018-03-21 00:00:00

  • Responses to Hydric Stress in the Seed-Borne Necrotrophic Fungus Alternaria brassicicola.

    abstract::Alternaria brassicicola is a necrotrophic fungus causing black spot disease and is an economically important seed-borne pathogen of cultivated brassicas. Seed transmission is a crucial component of its parasitic cycle as it promotes long-term survival and dispersal. Recent studies, conducted with the Arabidopsis thali...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01969

    authors: N'Guyen GQ,Raulo R,Marchi M,Agustí-Brisach C,Iacomi B,Pelletier S,Renou JP,Bataillé-Simoneau N,Campion C,Bastide F,Hamon B,Mouchès C,Porcheron B,Lemoine R,Kwasiborski A,Simoneau P,Guillemette T

    更新日期:2019-08-30 00:00:00

  • May the Phage be With You? Prophage-Like Elements in the Genomes of Soft Rot Pectobacteriaceae: Pectobacterium spp. and Dickeya spp.

    abstract::Soft Rot Pectobacteriaceae (SRP; Pectobacterium spp. and Dickeya spp., formerly known as pectinolytic Erwinia spp.) are necrotrophic bacterial pathogens infecting a large number of plant species worldwide, including agriculturally-important crops. Despite the SRP importance in agriculture, little is known about the ba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00138

    authors: Czajkowski R

    更新日期:2019-02-14 00:00:00

  • Meta-Analysis of Vaginal Microbiome Data Provides New Insights Into Preterm Birth.

    abstract::Preterm birth (PTB) is defined as the birth of an infant before 37 weeks of gestational age. It is the leading cause of perinatal morbidity and mortality worldwide. In this study, we present a comprehensive meta-analysis of vaginal microbiome in PTB. We integrated raw longitudinal 16S rRNA vaginal microbiome data from...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00476

    authors: Kosti I,Lyalina S,Pollard KS,Butte AJ,Sirota M

    更新日期:2020-04-08 00:00:00

  • A Genetic System for Methanocaldococcus jannaschii: An Evolutionary Deeply Rooted Hyperthermophilic Methanarchaeon.

    abstract::Phylogenetically deeply rooted methanogens belonging to the genus of Methanocaldococcus living in deep-sea hydrothermal vents derive energy exclusively from hydrogenotrophic methanogenesis, one of the oldest respiratory metabolisms on Earth. These hyperthermophilic, autotrophic archaea synthesize their biomolecules fr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01256

    authors: Susanti D,Frazier MC,Mukhopadhyay B

    更新日期:2019-07-03 00:00:00

  • Analysis of a Marseillevirus Transcriptome Reveals Temporal Gene Expression Profile and Host Transcriptional Shift.

    abstract::Marseilleviruses comprise a family of large double-stranded DNA viruses belonging to the proposed order "Megavirales." These viruses have a circular genome of ∼370 kbp, coding hundreds of genes. Over a half of their genes are associated with AT-rich putative promoter motifs, which have been demonstrated to be importan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00651

    authors: Rodrigues RAL,Louazani AC,Picorelli A,Oliveira GP,Lobo FP,Colson P,La Scola B,Abrahão JS

    更新日期:2020-04-14 00:00:00

  • Molecular Characteristics of Carbapenem-Resistant Enterobacter cloacae in Ningxia Province, China.

    abstract::The emergence of carbapenem-resistant Enterobacteriaceae (CRE) has become a major public health concern worldwide and a new challenge in the treatment of infectious diseases. The molecular characteristics of Enterobacter cloacae in Ningxia China are unknown. In this study, we reported 10 carbapenem-resistant E. cloaca...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00094

    authors: Shi Z,Zhao H,Li G,Jia W

    更新日期:2017-01-31 00:00:00

  • Type IVB Secretion Systems of Legionella and Other Gram-Negative Bacteria.

    abstract::Type IV secretion systems (T4SSs) play a central role in the pathogenicity of many important pathogens, including Agrobacterium tumefaciens, Helicobacter pylori, and Legionella pneumophila. The T4SSs are related to bacterial conjugation systems, and are classified into two subgroups, type IVA (T4ASS) and type IVB (T4B...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00136

    authors: Nagai H,Kubori T

    更新日期:2011-06-27 00:00:00

  • Viral Lysis of Photosynthesizing Microbes As a Mechanism for Calcium Carbonate Nucleation in Seawater.

    abstract::Removal of carbon through the precipitation and burial of calcium carbonate in marine sediments constitutes over 70% of the total carbon on Earth and is partitioned between coastal and pelagic zones. The precipitation of authigenic calcium carbonate in seawater, however, has been hotly debated because despite being in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01958

    authors: Lisle JT,Robbins LL

    更新日期:2016-12-09 00:00:00

  • Biosynthesis of Alkylcitric Acids in Aspergillus niger Involves Both Co-localized and Unlinked Genes.

    abstract::Filamentous fungi are an abundant source of bioactive secondary metabolites (SMs). In many cases, the biosynthetic processes of SMs are not well understood. This work focuses on a group of SMs, the alkylcitric acids, each of which contains a saturated alkyl "tail," and a citrate-derived "head." We initially identified...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01378

    authors: Palys S,Pham TTM,Tsang A

    更新日期:2020-06-30 00:00:00

  • Transcriptome Profile of Yeast Strain Used for Biological Wine Aging Revealed Dynamic Changes of Gene Expression in Course of Flor Development.

    abstract::Flor strains of Saccharomyces cerevisiae are principal microbial agents responsible for biological wine aging used for production of sherry-like wines. The flor yeast velum formed on the surface of fortified fermented must is a major adaptive and technological characteristic of flor yeasts that helps them to withstand...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00538

    authors: Mardanov AV,Eldarov MA,Beletsky AV,Tanashchuk TN,Kishkovskaya SA,Ravin NV

    更新日期:2020-04-03 00:00:00

  • Corrigendum: Evaluation of Human Milk Microbiota by 16S rRNA Gene Next-Generation Sequencing (NGS) and Cultivation/MALDI-TOF Mass Spectrometry Identification.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2019.02612.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2020.00031

    authors: Treven P,Mahnič A,Rupnik M,Golob M,Pirš T,Matijašić BB,Lorbeg PM

    更新日期:2020-01-29 00:00:00

  • Hyper-Osmotic Stress Elicits Membrane Depolarization and Decreased Permeability in Halotolerant Marine Debaryomyces hansenii Strains and in Saccharomyces cerevisiae.

    abstract::The use of seawater and marine microorganisms can represent a sustainable alternative to avoid large consumption of freshwater performing industrial bioprocesses. Debaryomyces hansenii, which is a known halotolerant yeast, possess metabolic traits appealing for developing such processes. For this purpose, we studied s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00064

    authors: Capusoni C,Arioli S,Donzella S,Guidi B,Serra I,Compagno C

    更新日期:2019-01-29 00:00:00

  • Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome.

    abstract::The human gut microbiome harbors a diverse array of metabolic pathways contributing to its development and homeostasis via a complex web of diet-dependent metabolic interactions within the microbial community and host. Genomics-based reconstruction and predictive modeling of these interactions would provide a framewor...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01316

    authors: Rodionov DA,Arzamasov AA,Khoroshkin MS,Iablokov SN,Leyn SA,Peterson SN,Novichkov PS,Osterman AL

    更新日期:2019-06-12 00:00:00