Antimicrobial Resistance and Molecular Epidemiology of ESBL-Producing Escherichia coli Isolated from Outpatients in Town Hospitals of Shandong Province, China.

Abstract:

:This study aimed to investigate antimicrobial resistance and molecular epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) isolated from outpatients in town hospitals of Shandong province, China. Antimicrobial susceptibility of ESBL-producing E. coli was tested using the disk diffusion and resistance genes encoding for β-lactamases (blaTEM, blaCTXM, and blaSHV) were detected by polymerase chain reaction (PCR). Multilocus sequence typing (ST) of ESBL-producing E. coli was analyzed in this study. Our results showed that of 320 E. coli isolates, 201 carried ESBL genes (201/320, 62.8%), and these isolates all carried blaCTX-M genes, the most common being blaCTX-M-14 (116/201, 57.7%), followed by blaCTX-M-55 (47/201, 23.4%) and blaCTX-M-15 (31/201, 15.4%). ESBL-producing E. coli exhibited highly resistant to penicillin derivatives, fluoroquinolones, folate pathway inhibitors, and third-generation cephalosporins, but no carbapenem-resistant isolates were found in this study. Forty-two STs were found among the 201 ESBL-producing E. coli, and the most common ST was ST131 (27/201, 13.4%), followed by ST405 (19/201, 9.5%) and ST69 (15/201, 7.5%). Taken together, a high isolation rate of ESBL-producing E. coli (62.8%) was found among outpatients in town hospitals. blaCTX-M gene was most dominant and was composed of a variety of subtypes. No dominant ST was detected among ESBL-producing E. coli, indicating that these ESBL-producing E. coli isolates derive from different clones.

journal_name

Front Microbiol

authors

Miao Z,Li S,Wang L,Song W,Zhou Y

doi

10.3389/fmicb.2017.00063

subject

Has Abstract

pub_date

2017-01-24 00:00:00

pages

63

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Enterotoxigenicity and Antimicrobial Resistance of Staphylococcus aureus Isolated from Retail Food in China.

    abstract::Staphylococcus aureus is one of the most common causes of zoonotic agent in the world, which are attributable to the contamination of food with enterotoxins. In this study, a total of 1,150 S. aureus isolates were cultured from 27,000 retail foods items from 203 cities of 24 provinces in China in 2015 and were test fo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02256

    authors: Wang W,Baloch Z,Jiang T,Zhang C,Peng Z,Li F,Fanning S,Ma A,Xu J

    更新日期:2017-11-21 00:00:00

  • Exploring Microdiversity in Novel Kordia sp. (Bacteroidetes) with Proteorhodopsin from the Tropical Indian Ocean via Single Amplified Genomes.

    abstract::Marine Bacteroidetes constitute a very abundant bacterioplankton group in the oceans that plays a key role in recycling particulate organic matter and includes several photoheterotrophic members containing proteorhodopsin. Relatively few marine Bacteroidetes species have been described and, moreover, they correspond t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01317

    authors: Royo-Llonch M,Ferrera I,Cornejo-Castillo FM,Sánchez P,Salazar G,Stepanauskas R,González JM,Sieracki ME,Speich S,Stemmann L,Pedrós-Alió C,Acinas SG

    更新日期:2017-07-25 00:00:00

  • Corrigendum: Cross-Talk between Staphylococcus aureus and Other Staphylococcal Species via the agr Quorum Sensing System.

    abstract::[This corrects the article on p. 1733 in vol. 7, PMID: 27877157.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2017.01075

    authors: Canovas J,Baldry M,Bojer MS,Andersen PS,Grzeskowiak PK,Stegger M,Damborg P,Olsen CA,Ingmer H

    更新日期:2017-06-08 00:00:00

  • Responses to Hydric Stress in the Seed-Borne Necrotrophic Fungus Alternaria brassicicola.

    abstract::Alternaria brassicicola is a necrotrophic fungus causing black spot disease and is an economically important seed-borne pathogen of cultivated brassicas. Seed transmission is a crucial component of its parasitic cycle as it promotes long-term survival and dispersal. Recent studies, conducted with the Arabidopsis thali...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01969

    authors: N'Guyen GQ,Raulo R,Marchi M,Agustí-Brisach C,Iacomi B,Pelletier S,Renou JP,Bataillé-Simoneau N,Campion C,Bastide F,Hamon B,Mouchès C,Porcheron B,Lemoine R,Kwasiborski A,Simoneau P,Guillemette T

    更新日期:2019-08-30 00:00:00

  • Functional Biology and Molecular Mechanisms of Host-Pathogen Interactions for Aflatoxin Contamination in Groundnut (Arachis hypogaea L.) and Maize (Zea mays L.).

    abstract::Aflatoxins are secondary metabolites produced by soilborne saprophytic fungus Aspergillus flavus and closely related species that infect several agricultural commodities including groundnut and maize. The consumption of contaminated commodities adversely affects the health of humans and livestock. Aflatoxin contaminat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.00227

    authors: Soni P,Gangurde SS,Ortega-Beltran A,Kumar R,Parmar S,Sudini HK,Lei Y,Ni X,Huai D,Fountain JC,Njoroge S,Mahuku G,Radhakrishnan T,Zhuang W,Guo B,Liao B,Singam P,Pandey MK,Bandyopadhyay R,Varshney RK

    更新日期:2020-03-03 00:00:00

  • Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses.

    abstract::In the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indicators with microbiol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00644

    authors: Stagnari F,Perpetuini G,Tofalo R,Campanelli G,Leteo F,Della Vella U,Schirone M,Suzzi G,Pisante M

    更新日期:2014-12-10 00:00:00

  • 5-Aminosalicylic Acid Alters the Gut Bacterial Microbiota in Patients With Ulcerative Colitis.

    abstract::Background: The aim of this study was to clarify the effect of 5-aminosalicylic acid (5-ASA) treatment on gut bacterial microbiota in patients with ulcerative colitis (UC). Methods: A total of 57 UC patients, including 20 untreated and 37 5-ASA-treated, were recruited into an exploration cohort. We endoscopically coll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01274

    authors: Xu J,Chen N,Wu Z,Song Y,Zhang Y,Wu N,Zhang F,Ren X,Liu Y

    更新日期:2018-06-13 00:00:00

  • Lactococcin B Is Inactivated by Intrinsic Proteinase PrtP Digestion in Lactococcus lactis subsp. lactis BGMN1-501.

    abstract::In our previous study we demonstrated that proteinase PrtP is able to impair bacteriocin LcnB activity, despite being produced by the same organism and encoded by the same plasmid. However, precise mechanism of this action, i.e., the exact cleavage site within LcnB bacteriocin, as well as its effect on antimicrobial a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00874

    authors: Vukotic G,Polovic N,Mirkovic N,Jovcic B,Stanisavljevic N,Fira D,Kojic M

    更新日期:2019-04-24 00:00:00

  • Microbial community composition of transiently wetted Antarctic Dry Valley soils.

    abstract::During the summer months, wet (hyporheic) soils associated with ephemeral streams and lake edges in the Antarctic Dry Valleys (DVs) become hotspots of biological activity and are hypothesized to be an important source of carbon and nitrogen for arid DV soils. Recent research in the DV has focused on the geochemistry a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00009

    authors: Niederberger TD,Sohm JA,Gunderson TE,Parker AE,Tirindelli J,Capone DG,Carpenter EJ,Cary SC

    更新日期:2015-01-28 00:00:00

  • The Outer Membrane Protein OmpW Enhanced V. cholerae Growth in Hypersaline Conditions by Transporting Carnitine.

    abstract::Pathogenic marine bacteria are found in environments and food sources with high salt concentrations, which the bacteria must effectively manage for their survival. Several mechanisms, such as the transport of ions and compatible solutes as well as changes in aerobic and anaerobic respiration, confer salt tolerance to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02703

    authors: Fu X,Zhang J,Li T,Zhang M,Li J,Kan B

    更新日期:2018-01-22 00:00:00

  • Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly-3-Hydroxybutyrate as Amendment.

    abstract::This study investigated the organohalide-respiring bacteria (OHRB) and the supporting microbial populations operating in a pilot scale plant employing poly-3-hydroxybutyrate (PHB), a biodegradable polymer produced by bacteria from waste streams, for the in situ bioremediation of groundwater contaminated by chlorinated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01664

    authors: Matturro B,Pierro L,Frascadore E,Petrangeli Papini M,Rossetti S

    更新日期:2018-07-24 00:00:00

  • Evaluation of MALDI-ToF Mass Spectrometry for Rapid Detection of Cereulide From Bacillus cereus Cultures.

    abstract::Bacillus cereus plays an often unrecognized role in food borne diseases. Food poisoning caused by this pathogen is manifested by either diarrhea or emesis. Due to the relatively high prevalence of emetic toxin cereulide associated food poisoning, methods for simple and reliable detection of cereulide producing strains...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.511674

    authors: Doellinger J,Schneider A,Stark TD,Ehling-Schulz M,Lasch P

    更新日期:2020-10-06 00:00:00

  • Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential.

    abstract::Digestive-tract microbiota exert tremendous influence over host health. Host-symbiont model systems are studied to investigate how symbioses are initiated and maintained, as well as to identify host processes affected by resident microbiota. The medicinal leech, Hirudo verbana, is an excellent model to address such qu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01569

    authors: Marden JN,McClure EA,Beka L,Graf J

    更新日期:2016-10-13 00:00:00

  • Real-Time PCR Quantification and Diversity Analysis of the Functional Genes aprA and dsrA of Sulfate-Reducing Prokaryotes in Marine Sediments of the Peru Continental Margin and the Black Sea.

    abstract::Sulfate-reducing prokaryotes (SRP) are ubiquitous and quantitatively important members in many ecosystems, especially in marine sediments. However their abundance and diversity in subsurface marine sediments is poorly understood. In this study, the abundance and diversity of the functional genes for the enzymes adenos...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00253

    authors: Blazejak A,Schippers A

    更新日期:2011-12-22 00:00:00

  • FDA Approved Drug Library Screening Identifies Robenidine as a Repositionable Antifungal.

    abstract::Due to the increasing prevalence of pathogenic fungal infections, the emergence of antifungal resistant clinical isolates worldwide, and the limited arsenal of available antifungals, developing new antifungal strategies is imperative. In this study, we screened a library of 1068 FDA-approved drugs to identify hits tha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00996

    authors: Mei Y,Jiang T,Zou Y,Wang Y,Zhou J,Li J,Liu L,Tan J,Wei L,Li J,Dai H,Peng Y,Zhang L,Lopez-Ribot JL,Shapiro RS,Chen C,Liu NN,Wang H

    更新日期:2020-06-03 00:00:00

  • Light-driven increase in carbon yield is linked to maintenance in the proteorhodopsin-containing Photobacterium angustum S14.

    abstract::A type of photoheterotrophic bacteria contain a transmembrane light-driven proton pump called proteorhodopsins (PRs). Due to the prevalence of these organisms in the upper water column of the World's Ocean, and their potential for light-driven ATP generation, they have been suggested to significantly influence energy ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00688

    authors: Courties A,Riedel T,Rapaport A,Lebaron P,Suzuki MT

    更新日期:2015-07-10 00:00:00

  • Adaptation to Bleaching: Are Thermotolerant Symbiodiniaceae Strains More Successful Than Other Strains Under Elevated Temperatures in a Model Symbiotic Cnidarian?

    abstract::The ability of some symbiotic cnidarians to resist and better withstand stress factors that cause bleaching is a trait that is receiving increased attention. The adaptive bleaching hypothesis postulates that cnidarians that can form a stable symbiosis with thermotolerant Symbiodiniaceae strains may cope better with in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00822

    authors: Newkirk CR,Frazer TK,Martindale MQ,Schnitzler CE

    更新日期:2020-05-05 00:00:00

  • Adaptation of Lactobacillus casei Zhang to Gentamycin Involves an Alkaline Shock Protein.

    abstract::Lactobacillus (L. casei) Zhang is a koumiss-originated probiotic strain, which was used as a model in a long-term antibiotics-driven evolution experiment to reveal bacterial evolutionary dynamics; and we isolated gentamycin-resistant L. casei Zhang descendents. To decipher the gentamycin resistance mechanism, here we ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02316

    authors: Zhang W,Guo H,Cao C,Li L,Kwok LY,Zhang H,Sun Z

    更新日期:2017-11-23 00:00:00

  • Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design.

    abstract::In order to eliminate the widespread use of antibiotics in livestock production, the research for alternatives has increased lately. This study examined the safety of 40 lactic acid bacteria (LAB) isolated from bovine feedlot environment and previously selected as potential probiotics. A high sensitivity prevalence to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02220

    authors: Aristimuño Ficoseco C,Mansilla FI,Maldonado NC,Miranda H,Fátima Nader-Macias ME,Vignolo GM

    更新日期:2018-09-28 00:00:00

  • Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues.

    abstract::Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other ph...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02266

    authors: de Noronha L,Zanluca C,Burger M,Suzukawa AA,Azevedo M,Rebutini PZ,Novadzki IM,Tanabe LS,Presibella MM,Duarte Dos Santos CN

    更新日期:2018-09-25 00:00:00

  • A TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens.

    abstract::Acetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood-Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks. Although most biochemical details of the WLP are well understood and systems-level characterization of acetogen metab...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02549

    authors: de Souza Pinto Lemgruber R,Valgepea K,Gonzalez Garcia RA,de Bakker C,Palfreyman RW,Tappel R,Köpke M,Simpson SD,Nielsen LK,Marcellin E

    更新日期:2019-11-15 00:00:00

  • Emerging Trends in Biological Treatment of Wastewater From Unconventional Oil and Gas Extraction.

    abstract::Unconventional oil and gas exploration generates an enormous quantity of wastewater, commonly referred to as flowback and produced water (FPW). Limited freshwater resources and stringent disposal regulations have provided impetus for FPW reuse. Organic and inorganic compounds released from the shale/brine formation, m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.569019

    authors: Acharya SM,Chakraborty R,Tringe SG

    更新日期:2020-09-09 00:00:00

  • Colonization of Mice With Amoxicillin-Associated Klebsiella variicola Drives Inflammation via Th1 Induction and Treg Inhibition.

    abstract::β-Lactam antibiotics can increase the resistance and virulence of individual intestinal microorganisms, which may affect host physiology and health. Klebsiella, a crucial gut inhabitant, has been confirmed to be resistant to most β-lactam antibiotics and contributes to the etiology of inflammatory bowel disease (IBD)....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01256

    authors: Lin H,Wang Q,Liu L,Chen Z,Das R,Zhao Y,Mao D,Luo Y

    更新日期:2020-06-24 00:00:00

  • Comparative Analysis of Gut Microbiota Changes in Père David's Deer Populations in Beijing Milu Park and Shishou, Hubei Province in China.

    abstract::This study used 16S rRNA high-throughput sequencing technology to examine the differences in gut microbiota between the Père David's deer populations in the Beijing and Shishou areas of China in order to understand the effects of ex situ conservation on the intestinal microflora in the Père David's deer. Results: On t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01258

    authors: Zhang M,Shi M,Fan M,Xu S,Li Y,Zhang T,Cha M,Liu Y,Guo X,Chen Q,Li Y,Zhang S,Hu D,Liu S

    更新日期:2018-06-12 00:00:00

  • Engineering nanoparticles to silence bacterial communication.

    abstract::The alarming spread of bacterial resistance to traditional antibiotics has warranted the study of alternative antimicrobial agents. Quorum sensing (QS) is a chemical cell-to-cell communication mechanism utilized by bacteria to coordinate group behaviors and establish infections. QS is integral to bacterial survival, a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00189

    authors: Miller KP,Wang L,Chen YP,Pellechia PJ,Benicewicz BC,Decho AW

    更新日期:2015-03-10 00:00:00

  • The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response of Listeria monocytogenes.

    abstract::Listeria monocytogenes is a foodborne pathogen that can cause severe disease in susceptible humans. This microorganism has the ability to adapt to hostile environmental conditions such as the low temperatures used by the food industry for controlling microorganisms. Bacteria are able to adjust their transcriptional re...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00612

    authors: Quesille-Villalobos AM,Parra A,Maza F,Navarrete P,González M,Latorre M,Toro M,Reyes-Jara A

    更新日期:2019-03-28 00:00:00

  • A Novel MSCRAMM Subfamily in Coagulase Negative Staphylococcal Species.

    abstract::Coagulase negative staphylococci (CoNS) are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major vi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00540

    authors: Arora S,Uhlemann AC,Lowy FD,Hook M

    更新日期:2016-04-29 00:00:00

  • Corrigendum: Foam Cells Control Mycobacterium tuberculosis Infection.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2020.01394.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2020.594142

    authors: Agarwal P,Combes TW,Shojaee-Moradie F,Fielding B,Gordon S,Mizrahi V,Martinez FO

    更新日期:2020-10-27 00:00:00

  • Dynamics Relationship of Phyllosphere and Rhizosphere Bacterial Communities During the Development of Bothriochloa ischaemum in Copper Tailings.

    abstract::Copper mining and the byproducts associated with the industry have led to serious pollution in the Loess Plateau of China. There is a potential in improving the ecological restoration efficiency of such degraded land through combining microbial and plant remediation approaches. However, the community structure and fun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00869

    authors: Jia T,Yao Y,Wang R,Wu T,Chai B

    更新日期:2020-05-28 00:00:00

  • The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans.

    abstract::Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker's yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00047

    authors: Aerts AM,Bammens L,Govaert G,Carmona-Gutierrez D,Madeo F,Cammue BP,Thevissen K

    更新日期:2011-03-16 00:00:00