An Alternative Bioassay for Synchytrium endobioticum Demonstrates the Expression of Potato Wart Resistance in Aboveground Plant Parts.

Abstract:

:The obligate biotrophic chytrid species Synchytrium endobioticum is the causal agent of potato wart disease. Currently, 39 pathotypes have been described based on their interaction with a differential set of potato varieties. Wart resistance and pathotyping is performed using bioassays in which etiolated tuber sprouts are inoculated. Here, we describe an alternative method in which aboveground plant parts are inoculated. Susceptible plants produced typical wart symptoms in developing but not in fully expanded aboveground organs. Colonization of the host by S. endobioticum was verified by screening for resting spores by microscopy and by molecular techniques using TaqMan polymerase chain reaction and RNAseq analysis. When applied to resistant plants, none of these symptoms were detectable. Recognition of S. endobioticum pathotypes by differentially resistant potato varieties was identical in axillary buds and the tuber-based bioassays. This suggests that S. endobioticum resistance genes are expressed in both etiolated "belowground" sprouts and green aboveground organs. RNAseq analysis demonstrated that the symptomatic aboveground materials contain less contaminants compared with resting spores extracted from tuber-based assays. This reduced microbial contamination in the aboveground bioassay could be an important advantage to study this obligate biotrophic plant-pathogen interaction. Because wart resistance is active in both below- and aboveground organs, the aboveground bioassay can potentially speed up screening for S. endobioticum resistance in potato breeding programs because it omits the requirement for tuber formation. In addition, possibilities arise to express S. endobioticum effectors in potato leaves through agroinfiltration, thereby providing additional phenotyping tools for research and breeding. Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

journal_name

Phytopathology

journal_title

Phytopathology

authors

van de Vossenberg BTLH,van Gent-Pelzer MPE,Boerma M,van der Gouw LP,van der Lee TAJ,Vossen JH

doi

10.1094/PHYTO-01-19-0024-R

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

1043-1052

issue

6

eissn

0031-949X

issn

1943-7684

journal_volume

109

pub_type

杂志文章
  • Rhizobacteria-Mediated Growth Promotion of Tomato Leads to Protection Against Cucumber mosaic virus.

    abstract::ABSTRACT We evaluated combinations of two strains of plant growth-promoting rhizobacteria (PGPR) formulated with the carrier chitosan for the ability to induce growth promotion of tomato plants and resistance to infection by Cucumber mosaic virus (CMV). Each PGPR combination included GB03 (Bacillus subtilis) and one o...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2003.93.10.1301

    authors: Murphy JF,Reddy MS,Ryu CM,Kloepper JW,Li R

    更新日期:2003-10-01 00:00:00

  • Improved sampling methods for real-time polymerase chain reaction diagnosis of citrus canker from field samples.

    abstract::ABSTRACT Citrus bacterial canker disease has been introduced at least three times into Florida in the last 15 years and, despite federal and state quarantine and eradication efforts, continues to spread in Florida. Accurate, fast, and reliable detection of the causal agent is of great importance. However, citrus bacte...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.1.61

    authors: Mavrodieva V,Levy L,Gabriel DW

    更新日期:2004-01-01 00:00:00

  • F240 of β2-Tubulin Explains why Fusarium graminearum is Less Sensitive to Carbendazim than Botrytis cinerea.

    abstract::β-Tubulin is the target of benzimidazole fungicides, the most widely used of which is carbendazim (methyl benzimidazol-2-ylcarbamate [MBC]). MBC sensitivity is determined by the differential affinity of MBC for β-tubulins. However, the mechanism of less sensitivity of Fusarium graminearum to MBC compared with other fu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-17-0295-R

    authors: Zhu Y,Liang X,Li Y,Duan Y,Zheng Z,Wang J,Zhou M

    更新日期:2018-03-01 00:00:00

  • Quantitative Real-Time PCR Analysis of Individual Flue-Cured Tobacco Seeds and Seedlings Reveals Seed Transmission of Tobacco Mosaic Virus.

    abstract::Tobacco mosaic virus (TMV) is an extensively studied RNA virus known to infect tobacco (Nicotiana tabacum) and other solanaceous crops. TMV has been classified as a seedborne virus in tobacco, with infection of developing seedlings thought to occur from contact with the TMV-infected seed coat. The mechanism of TMV tra...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-06-19-0201-FI

    authors: Ellis MD,Hoak JM,Ellis BW,Brown JA,Sit TL,Wilkinson CA,Reed TD,Welbaum GE

    更新日期:2020-01-01 00:00:00

  • Mechanism of Action of the Benzimidazole Fungicide on Fusarium graminearum: Interfering with Polymerization of Monomeric Tubulin But Not Polymerized Microtubule.

    abstract::Tubulins are the proposed target of clinically relevant anticancer drugs, anthelmintic, and fungicide. β2-tubulin of the plant pathogen Fusarium graminearum was considered as the target of benzimidazole compounds by homology modeling in our previous work. In this study, α1-, α2-, and β2-tubulin of F. graminearum were ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-15-0186-R

    authors: Zhou Y,Xu J,Zhu Y,Duan Y,Zhou M

    更新日期:2016-08-01 00:00:00

  • Genetic diversity and potential vectors and reservoirs of Cucurbit aphid-borne yellows virus in southeastern Spain.

    abstract::The genetic variability of a Cucurbit aphid-borne yellows virus (CABYV) (genus Polerovirus, family Luteoviridae) population was evaluated by determining the nucleotide sequences of two genomic regions of CABYV isolates collected in open-field melon and squash crops during three consecutive years in Murcia (southeaster...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-11-12-0280-R

    authors: Kassem MA,Juarez M,Gómez P,Mengual CM,Sempere RN,Plaza M,Elena SF,Moreno A,Fereres A,Aranda MA

    更新日期:2013-11-01 00:00:00

  • Disease-weather relationships for powdery mildew and yellow rust on winter wheat.

    abstract::Key weather factors determining the occurrence and severity of powdery mildew and yellow rust epidemics on winter wheat were identified. Empirical models were formulated to qualitatively predict a damaging epidemic (>5% severity) and quantitatively predict the disease severity given a damaging epidemic occurred. The d...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-5-0609

    authors: Te Beest DE,Paveley ND,Shaw MW,van den Bosch F

    更新日期:2008-05-01 00:00:00

  • Fine Mapping of a New Race-Specific Blast Resistance Gene, Pi-hk2, in Japonica Heikezijing from Taihu Region of China.

    abstract::Heikezijing, a japonica rice landrace from the Taihu region of China, exhibited broad-spectrum resistance to more than 300 isolates of the blast pathogen (Magnaporthe oryzae). In our previous research, we fine mapped a broad-spectrum resistance gene, Pi-hk1, in chromosome 11. In this research, 2010-9(G1), one of the p...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-03-16-0151-R

    authors: He W,Fang N,Wang R,Wu Y,Zeng G,Guan C,Chen H,Huang J,Wang J,Bao Y,Zhang H

    更新日期:2017-01-01 00:00:00

  • Frequency of the Ht1 Gene in Populations of Sweet Corn Selected for Resistance to Exserohilum turcicum Race 1.

    abstract::ABSTRACT The possibility that the Ht1 gene or genes tightly linked to Ht1 convey general resistance to races of Exserohilum turcicum that are virulent against Ht1 (i.e., residual resistance) could be useful in sweet corn where the Ht1 gene is present in many commercial hybrids and breeding populations. The objective o...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0085

    authors: Campaña A,Pataky JK

    更新日期:2005-01-01 00:00:00

  • Latency and Persistence of 'Candidatus Liberibacter asiaticus' in Its Psyllid Vector, Diaphorina citri (Hemiptera: Liviidae).

    abstract::Although 'Candidatus Liberibacter asiaticus' (Las) is a major pathogen associated with citrus huanglongbing (HLB), some characteristics of transmission by the psyllid vector Diaphorina citri are not fully understood. We examined the latent period and persistence of transmission of Las by D. citri in a series of experi...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-16-0088-R

    authors: Canale MC,Tomaseto AF,Haddad ML,Della Coletta-Filho H,Lopes JR

    更新日期:2017-03-01 00:00:00

  • Comparative effect of low temperature on virulence and twitching motility of Ralstonia solanacearum strains present in Florida.

    abstract::Ralstonia solanacearum causes bacterial wilt on a wide range of plant hosts. Most strains of R. solanacearum are nonpathogenic below 20°C; however, Race 3 Biovar 2 (R3B2) strains are classified as quarantine pathogens because of their ability to infect crops, cause disease, and survive in temperate climates. We have i...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-05-11-0145

    authors: Bocsanczy AM,Achenbach UC,Mangravita-Novo A,Yuen JM,Norman DJ

    更新日期:2012-02-01 00:00:00

  • Indicator Species Analysis: A Useful Tool for Plant Disease Studies.

    abstract::Indicator species analysis (ISA) uses indices of an organism's relative abundance and occurrence to estimate the strength of its associations with a priori groups of interest and a simple randomization test to evaluate the probability of association. Because ISA values tend to be greatest when a species is both relati...

    journal_title:Phytopathology

    pub_type: 信件

    doi:10.1094/PHYTO-12-19-0462-LE

    authors: Severns PM,Sykes EM

    更新日期:2020-12-01 00:00:00

  • A Molecular Marker Correlated with Selected Virulence Against the Tomato Resistance Gene Mi in Meloidogyne incognita, M. javanica, and M. arenaria.

    abstract::ABSTRACT Root-knot nematodes of the genus Meloidogyne are economically important pathogens of a wide range of crops. The tomato resistance gene Mi typically confers resistance to the three major species, M. incognita, M. javanica, and M. arenaria. However, virulent populations completely overcoming the Mi resistance s...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2001.91.4.377

    authors: Xu J,Narabu T,Mizukubo T,Hibi T

    更新日期:2001-04-01 00:00:00

  • Virulence Differences in Blumeria graminis f. sp. tritici from the Central and Eastern United States.

    abstract::Wheat powdery mildew is a disease of global importance that occurs across a wide geographic area in the United States. A virulence survey of Blumeria graminis f. sp. tritici, the causal agent, was conducted by sampling 36 wheat fields in 15 U.S. states in the years 2013 and 2014. Using a hierarchical sampling protocol...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-06-17-0211-R

    authors: Cowger C,Mehra L,Arellano C,Meyers E,Murphy JP

    更新日期:2018-03-01 00:00:00

  • One Cut to Change Them All: CRISPR/Cas, a Groundbreaking Tool for Genome Editing in Botrytis cinerea and Other Fungal Plant Pathogens.

    abstract::CRISPR/Cas is a genome editing technology that has opened new dimensions in functional biology. In a recent publication, we presented a highly efficient CRISPR/Cas technique for Botrytis cinerea, which dramatically increases our options to mutagenize and modify single or multiple genes. In this Perspectives article, w...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-20-0379-PER

    authors: Hahn M,Scalliet G

    更新日期:2021-01-29 00:00:00

  • trans-Dominant Inhibition of Geminiviral DNA Replication by Bean Golden Mosaic Geminivirus rep Gene Mutants.

    abstract::ABSTRACT Geminiviruses are a group of single-stranded DNA viruses that cause major losses on a number of important crops throughout the world. Bean golden mosaic virus (BGMV) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (Phaseolus vulgaris) in the Western Hemisphere. T...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.6.480

    authors: Hanson SF,Maxwell DP

    更新日期:1999-06-01 00:00:00

  • Sensitivity of Mycosphaerella fijiensis, Causal Agent of Black Sigatoka of Banana, to Propiconazole.

    abstract::ABSTRACT One hundred monoascosporic isolates of Mycosphaerella fijiensis were collected in February and November 1994 from each of two banana (Musa spp.) plantations in Costa Rica. Locations at San Pablo and Coopecariari had been sprayed with propiconazole for the past 7 years to control black Sigatoka. One hundred mo...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.1.96

    authors: Romero RA,Sutton TB

    更新日期:1997-01-01 00:00:00

  • Detection and Quantification of Resistance of Venturia inaequalis Populations to Sterol Demethylation Inhibitors.

    abstract::ABSTRACT Monoconidial isolates of Venturia inaequalis were collected in 1990 and 1991 from orchards in New York, Michigan, and Nova Scotia that had never or only sporadically been treated with fungicides acting as sterol demethylation inhibitors (DMIs). Sensitivities of isolates to two representative DMIs (fenarimol a...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.2.184

    authors: Köller W,Wilcox WF,Barnard J,Jones AL,Braun PG

    更新日期:1997-02-01 00:00:00

  • Effects of Date of Inoculation on the Within-Plant Movement of Xylella fastidiosa and Persistence of Pierce's Disease Within Field Grapevines.

    abstract::ABSTRACT The effects of date of inoculation on the development of Pierce's disease (PD) were evaluated in California grapevines during 1997 through 2000 at four locations. Some vines that had been inoculated either by using blue-green sharpshooters (Graphocephala atropunctata) as vectors or mechanically by needle punc...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2003.93.2.244

    authors: Feil H,Feil WS,Purcell AH

    更新日期:2003-02-01 00:00:00

  • Assessing changes and associations in the Xanthomonas perforans population across Florida commercial tomato fields via a state-wide survey.

    abstract::Before 1991, Xanthomonas euvesicatoria (Xe) was the causal agent of bacterial spot of tomato in Florida but was quickly replaced by Xanthomonas perforans (Xp). The Xp population has changed in genotype and phenotype despite lack of a clear selection pressure. To determine the current Xanthomonas population in Florida,...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-20-0402-R

    authors: Klein-Gordon J,Xing Y,Garrett KA,Abrahamian P,Paret ML,Minsavage GV,Strayer-Scherer AL,Fulton J,Timilsina S,Jones JB,Goss EM,Vallad GE

    更新日期:2020-10-13 00:00:00

  • Effect of Flumorph on F-Actin Dynamics in the Potato Late Blight Pathogen Phytophthora infestans.

    abstract::Oomycetes are fungal-like pathogens that cause notorious diseases. Protecting crops against oomycetes requires regular spraying with chemicals, many with an unknown mode of action. In the 1990s, flumorph was identified as a novel crop protection agent. It was shown to inhibit the growth of oomycete pathogens including...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-14-0119-R

    authors: Hua C,Kots K,Ketelaar T,Govers F,Meijer HJ

    更新日期:2015-04-01 00:00:00

  • The Role of a Host-Induced Arginase of Xanthomonas oryzae pv. oryzae in Promoting Virulence on Rice.

    abstract::The plant bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial blight of rice, which is one of the most destructive rice diseases prevalent in Asia and parts of Africa. Despite many years of research, how X. oryzae pv. oryzae causes bacterial blight of rice is still not completely understood. Here, we sho...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-19-0058-R

    authors: Zhang Y,Wu G,Palmer I,Wang B,Qian G,Fu ZQ,Liu F

    更新日期:2019-11-01 00:00:00

  • Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice.

    abstract::The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-12-0260-R

    authors: Lv Q,Xu X,Shang J,Jiang G,Pang Z,Zhou Z,Wang J,Liu Y,Li T,Li X,Xu J,Cheng Z,Zhao X,Li S,Zhu L

    更新日期:2013-06-01 00:00:00

  • Stepwise Evolution of Races in Fusarium oxysporum f. sp. ciceris Inferred from Fingerprinting with Repetitive DNA Sequences.

    abstract::ABSTRACT Plant pathogens often exhibit variation in virulence, the ability to cause disease on host plants with specific resistance, evident from the diversity of races observed within pathogen species. The evolution of races in asexual fungal pathogens has been hypothesized to occur in a stepwise fashion, in which mu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.3.228

    authors: Del Mar Jiménez-Gasco M,Milgroom MG,Jiménez-Díaz RM

    更新日期:2004-03-01 00:00:00

  • Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana.

    abstract::The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-6-0666

    authors: Barriuso J,Solano BR,Gutiérrez Mañero FJ

    更新日期:2008-06-01 00:00:00

  • Infection processes and involvement of defense-related genes in the expression of resistance in cultivars of subterranean clover (Trifolium subterraneum) to Phytophthora clandestina.

    abstract::Studies on infection processes and gene expression were done to determine differential responses of cultivars of Trifolium subterraneum resistant and susceptible to infection by races of Phytophthora clandestina. In the infection process study, one race was inoculated onto the roots of T. subterraneum cvs. Woogenellup...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-6-0551

    authors: Ma X,Li H,Sivasithamparam K,Barbetti MJ

    更新日期:2010-06-01 00:00:00

  • The population genetic structure of Rhizoctonia solani AG-3PT from potato in the Colombian Andes.

    abstract::The soilborne fungus Rhizoctonia solani anastomosis group 3 (AG-3PT) is a globally important potato pathogen. However, little is known about the population genetic processes affecting field populations of R. solani AG-3PT, especially in the South American Colombian Andes, which is near the center of diversity of the t...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-11-12-0278-R

    authors: Ferrucho RL,Ceresini PC,Ramirez-Escobar UM,McDonald BA,Cubeta MA,García-Domínguez C

    更新日期:2013-08-01 00:00:00

  • Genetic Diversity in Poplar Leaf Rust (Melampsora medusae f. sp. deltoidae) in the Zones of Host Sympatry and Allopatry.

    abstract::ABSTRACT Poplar leaf rust caused by Melampsora medusae f. sp. deltoidae is a widespread disease in North America, where epidemics occur within zones of sympatry and allopatry of telial hosts (Populus spp.) and aecial hosts (Larix spp.). To test the hypothesis that epidemics originate in the zone of sympatry where the ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-5-0603

    authors: Bourassa M,Bernier L,Hamelin RC

    更新日期:2007-05-01 00:00:00

  • Plump kernels with high deoxynivalenol linked to late Gibberella zeae infection and marginal disease conditions in winter wheat.

    abstract::Deoxynivalenol (DON) concentrations in mature wheat grain are usually correlated with symptoms produced by Gibberella zeae infection. However, there have been numerous observations of unacceptably high DON in asymptomatic crops, which can lead to lower-than-expected milling reductions in DON. We conducted a field expe...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-7-0719

    authors: Cowger C,Arrellano C

    更新日期:2010-07-01 00:00:00

  • Compost Tea as a Container Medium Drench for Suppressing Seedling Damping-Off Caused by Pythium ultimum.

    abstract::ABSTRACT Compost tea is being used increasingly in agricultural production to control plant diseases. However, there has been limited investigation relating disease control efficacy to various compost tea production methods, particularly compost tea produced with active aeration and additives to increase microbial pop...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.11.1156

    authors: Scheuerell SJ,Mahaffee WF

    更新日期:2004-11-01 00:00:00