当前位置： SCI文献检索 > 期刊下所有文献 > Macrolide resistance of Mycoplasma pneumoniae and its detection rate by real-time PCR in primary and tertiary care hospitals.

Macrolide resistance of Mycoplasma pneumoniae and its detection rate by real-time PCR in primary and tertiary care hospitals.

Abstract：

BACKGROUND:This study aimed to evaluate the prevalence of Mycoplasma pneumoniae in primary and tertiary care hospitals and its macrolide resistance rate. METHODS:Nasopharyngeal swabs were collected from 195 pediatric patients in primary and tertiary care hospitals from October to November 2010. The AccuPower MP real-time PCR kit (Bioneer, Korea) was used for the detection of M. pneumoniae. Direct amplicon sequencing was performed to detect point mutations conferring resistance to macrolides in the 23S rRNA gene. RESULTS:Among the 195 specimens, 17 (8.7%) were M. pneumoniae positive, and 3 of the strains (17.6%) obtained from these 17 specimens displayed the A2063G mutation in 23S rRNA. Three macrolide-resistant M. pneumoniae isolates were isolated from patients hospitalized at the primary care hospital. The positive rates of M. pneumoniae for the primary and tertiary care hospitals were 12.1% (15/124) and 2.8% (2/71), respectively (P=0.033). CONCLUSIONS:The positive rate of M. pneumoniae in the primary care hospital was higher than that in the tertiary care hospital. Simultaneous detection of M. pneumoniae and macrolide-resistant mutation genes in the 23S rRNA by real-time PCR is needed for rapid diagnosis and therapy of M. pneumoniae infections.

journal_name

Ann Lab Med

journal_title

Annals of laboratory medicine

authors

Uh Y,Hong JH,Oh KJ,Cho HM,Park SD,Kim J,Yoon KJ

doi

10.3343/alm.2013.33.6.410

keywords:

["23S rRNA","Antibiotic resistance","Macrolide","Mycoplasma pneumoniae"]

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

410-4

issue

6

eissn

2234-3806

issn

2234-3814

journal_volume

33

pub_type

杂志文章

相关文献

文献大全