Abstract：
:In this work, a low-cost alternative method (i.e., adding nitrate into WAS) to significantly enhance hydrogen production was reported. Experimental results showed that with an increase of nitrate addition from 0 to 362 mg/L, the maximal hydrogen production from acidic (pH 5.5) fermentation of WAS obviously increased from 12.6 ± 0.5 to 19.3 ± 0.9 mL per gram volatile suspended solids (VSS). The mechanism investigations illustrated more substrates were provided for subsequent hydrogen production. Although the nitrate added inhibited all the biological processes, its inhibition to the hydrogen consumption processes was much severer than that to the hydrogen production processes. The enzyme analyses on the long-term semi-continuous fermenters showed that the nitrate addition slightly inhibited the relative activities of protease, butyrate kinase, acetate kinase, CoA-transferase, and [FeFe] hydrogenase but largely suppressed the relative activities of coenzyme F420, carbon monoxide dehydrogenase, and adenylyl sulfate reductase.
journal_name
Chemospherejournal_title
Chemosphereauthors
Wang Y,Wang D,Chen F,Yang Q,Ni BJ,Wang Q,Sun J,Li X,Liu Ydoi
10.1016/j.chemosphere.2019.06.117subject
Has Abstractpub_date
2019-11-01 00:00:00pages
814-824eissn
0045-6535issn
1879-1298pii
S0045-6535(19)31358-Xjournal_volume
235pub_type
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