A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation.

Abstract:

:The yeasts belonging to the Wickerhamiella and Starmerella genera (W/S clade) share a distinctive evolutionary history marked by loss and subsequent reinstatement of alcoholic fermentation mediated by horizontal gene transfer events. Species in this clade also share unusual features of metabolism, namely the preference for fructose over glucose as carbon source, a rare trait known as fructophily. Here we show that fructose may be the preferred sugar in W/S-clade species because, unlike glucose, it can be converted directly to mannitol in a reaction with impact on redox balance. According to our results, mannitol is excreted to the growth medium in appreciable amounts along with other fermentation products such as glycerol and ethanol but unlike the latter metabolites mannitol production increases with temperature. We used comparative genomics to find genes involved in mannitol metabolism and established the mannitol biosynthesis pathway in W/S-clade species Starmerella bombicola using molecular genetics tools. Surprisingly, mannitol production seems to be so important that St. bombicola (and other W/S-clade species) deploys a novel pathway to mediate the conversion of glucose to fructose, thereby allowing cells to produce mannitol even when glucose is the sole carbon source. Using targeted mutations and 13C-labeled glucose followed by NMR analysis of end-products, we showed that the novel mannitol biosynthesis pathway involves fructose-6-phosphate as an intermediate, implying a key role for a yet unknown fructose-6-P phosphatase. We hypothesize that mannitol production contributed to mitigate the negative effects on redox balance of the ancient loss of alcoholic fermentation in the W/S clade. Presently, mannitol also seems to play a role in stress protection.

journal_name

Front Microbiol

authors

Gonçalves C,Ferreira C,Gonçalves LG,Turner DL,Leandro MJ,Salema-Oom M,Santos H,Gonçalves P

doi

10.3389/fmicb.2019.02510

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

2510

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Biogenesis, Function, and Applications of Virus-Derived Small RNAs in Plants.

    abstract::RNA silencing, an evolutionarily conserved and sequence-specific gene-inactivation system, has a pivotal role in antiviral defense in most eukaryotic organisms. In plants, a class of exogenous small RNAs (sRNAs) originating from the infecting virus called virus-derived small interfering RNAs (vsiRNAs) are predominantl...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.01237

    authors: Zhang C,Wu Z,Li Y,Wu J

    更新日期:2015-11-09 00:00:00

  • Insight Into the Diversity and Possible Role of Plasmids in the Adaptation of Psychrotolerant and Metalotolerant Arthrobacter spp. to Extreme Antarctic Environments.

    abstract::Arthrobacter spp. are coryneform Gram-positive aerobic bacteria, belonging to the class Actinobacteria. Representatives of this genus have mainly been isolated from soil, mud, sludge or sewage, and are usually mesophiles. In recent years, the presence of Arthrobacter spp. was also confirmed in various extreme, includi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03144

    authors: Romaniuk K,Golec P,Dziewit L

    更新日期:2018-12-18 00:00:00

  • Ionic Liquids as Unforeseen Assets to Fight Life-Threatening Mycotic Diseases.

    abstract::Ionic liquids discovery has celebrated 100 years. They consist solely of ions, one of which is typically organic and asymmetrical. Remarkable physical and chemical properties stirred their use as alternative solvents in many chemical processes. The recent demonstration of their occurrence in nature might boost their i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00111

    authors: Hartmann DO,Petkovic M,Silva Pereira C

    更新日期:2016-02-08 00:00:00

  • Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth.

    abstract::The plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass sp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01086

    authors: Long J,Basu Roy R,Zhang YJ,Antrobus R,Du Y,Smith DL,Weekes MP,Javid B

    更新日期:2016-07-12 00:00:00

  • Methane Emission in a Specific Riparian-Zone Sediment Decreased with Bioelectrochemical Manipulation and Corresponded to the Microbial Community Dynamics.

    abstract::Dissimilatory metal-reducing bacteria are widespread in terrestrial ecosystems, especially in anaerobic soils and sediments. Thermodynamically, dissimilatory metal reduction is more favorable than sulfate reduction and methanogenesis but less favorable than denitrification and aerobic respiration. It is critical to un...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01523

    authors: Friedman ES,McPhillips LE,Werner JJ,Poole AC,Ley RE,Walter MT,Angenent LT

    更新日期:2016-01-11 00:00:00

  • Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies.

    abstract::Sewage waste represents an ecosystem of complex and interactive microbial consortia which proliferate with different kinetics according to their individual genetic as well as metabolic potential. We performed metagenomic shotgun sequencing on Ion-Torrent platform, to explore the microbial community structure, their bi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01382

    authors: Sidhu C,Vikram S,Pinnaka AK

    更新日期:2017-07-19 00:00:00

  • Functional and evolutionary analyses of Helicobacter pylori HP0231 (DsbK) protein with strong oxidative and chaperone activity characterized by a highly diverged dimerization domain.

    abstract::Helicobacter pylori does not encode the classical DsbA/DsbB oxidoreductases that are crucial for oxidative folding of extracytoplasmic proteins. Instead, this microorganism encodes an untypical two proteins playing a role in disulfide bond formation - periplasmic HP0231, which structure resembles that of EcDsbC/DsbG, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01065

    authors: Bocian-Ostrzycka KM,Łasica AM,Dunin-Horkawicz S,Grzeszczuk MJ,Drabik K,Dobosz AM,Godlewska R,Nowak E,Collet JF,Jagusztyn-Krynicka EK

    更新日期:2015-10-08 00:00:00

  • Lipids in Aspergillus flavus-maize interaction.

    abstract::In some filamentous fungi, the pathways related to the oxidative stress and oxylipins production are involved both in the process of host-recognition and in the pathogenic phase. In fact, recent studies have shown that the production of oxylipins in filamentous fungi, yeasts and chromists is also related to the develo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00074

    authors: Scarpari M,Punelli M,Scala V,Zaccaria M,Nobili C,Ludovici M,Camera E,Fabbri AA,Reverberi M,Fanelli C

    更新日期:2014-02-27 00:00:00

  • The Two-Component System RsrS-RsrR Regulates the Tetrathionate Intermediate Pathway for Thiosulfate Oxidation in Acidithiobacillus caldus.

    abstract::Acidithiobacillus caldus (A. caldus) is a common bioleaching bacterium that possesses a sophisticated and highly efficient inorganic sulfur compound metabolism network. Thiosulfate, a central intermediate in the sulfur metabolism network of A. caldus and other sulfur-oxidizing microorganisms, can be metabolized via th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01755

    authors: Wang ZB,Li YQ,Lin JQ,Pang X,Liu XM,Liu BQ,Wang R,Zhang CJ,Wu Y,Lin JQ,Chen LX

    更新日期:2016-11-03 00:00:00

  • Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China.

    abstract::Incompatibility group C (IncC) plasmids have received attention due to their broad host range and because they harbor key antibiotic resistance genes. Because these resistance genes can spread from food-producing animals to human, the proliferation of these plasmids represents a public health risk. In this study, a to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.580960

    authors: Zhang Y,Lei CW,Chen X,Yao TG,Yu JW,Hu WL,Mao X,Wang HN

    更新日期:2020-10-27 00:00:00

  • Quorum Sensing Inhibiting Activity of Streptomyces coelicoflavus Isolated from Soil.

    abstract::Quorum sensing (QS) systems communicate bacterial population and stimulate microbial pathogenesis through signaling molecules. Inhibition of QS signals potentially suppresses microbial infections. Antimicrobial properties of Streptomyces have been extensively studied, however, less is known about quorum sensing inhibi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00659

    authors: Hassan R,Shaaban MI,Abdel Bar FM,El-Mahdy AM,Shokralla S

    更新日期:2016-05-13 00:00:00

  • Genome-Wide Identification and Classification of Soybean C2H2 Zinc Finger Proteins and Their Expression Analysis in Legume-Rhizobium Symbiosis.

    abstract::Root nodule symbiosis (RNS) is one of the most productive and economical systems for nitrogen fixation, and previous studies have shown that several nodule-specific C2H2-zinc finger proteins (ZFPs) play important roles in symbiosis establishment and nodule function. However, C2H2-ZFPs are the most widespread ZFPs in e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00126

    authors: Yuan S,Li X,Li R,Wang L,Zhang C,Chen L,Hao Q,Zhang X,Chen H,Shan Z,Yang Z,Chen S,Qiu D,Ke D,Zhou X

    更新日期:2018-02-06 00:00:00

  • Modulation of Plant Defense System in Response to Microbial Interactions.

    abstract::At different stages throughout their life cycle, plants often encounter several pathogenic microbes that challenge plant growth and development. The sophisticated innate plant immune system prevents the growth of harmful microbes via two interconnected defense strategies based on pathogen perception. These strategies ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01298

    authors: Nishad R,Ahmed T,Rahman VJ,Kareem A

    更新日期:2020-07-03 00:00:00

  • Biosurveillance of avian influenza and Newcastle disease viruses in the Barda region of Azerbaijan using real time RT-PCR and hemagglutination inhibition.

    abstract::The Azerbaijan State Veterinary Control Service (SVCS) has conducted active serological surveillance for avian influenza (AI) in poultry since 2006, when the first outbreak of AI H5N1 occurred in Azerbaijan. Samples are collected from September to May annually and tested using a hemagglutination inhibition (HI) assay ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01128

    authors: Zeynalova S,Guliyev F,Vatani M,Abbasov B

    更新日期:2015-11-06 00:00:00

  • Multiple Ribosomal RNA Operons in Bacteria; Their Concerted Evolution and Potential Consequences on the Rate of Evolution of Their 16S rRNA.

    abstract::Bacterial species differ greatly in the number and location of the rRNA operons which may be present in the bacterial chromosomes and plasmids. Most bacterial species contain more than one ribosomal RNA operon copy in their genomes, with some species containing up to 15 such copies. We review the number and location o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01232

    authors: Espejo RT,Plaza N

    更新日期:2018-06-08 00:00:00

  • Draft Genomic Analysis of an Avian Multidrug Resistant Morganella morganii Isolate Carrying qnrD1.

    abstract::Morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. We report the 4.3 Mbp draft genome sequence of a M. morganii isolated in association with an Escherichia coli from broilers in Portugal that showed macroscopic lesions consistent with colisepticemia....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01660

    authors: Jones-Dias D,Clemente L,Moura IB,Sampaio DA,Albuquerque T,Vieira L,Manageiro V,Caniça M

    更新日期:2016-10-25 00:00:00

  • Microbial diversity in a Venezuelan orthoquartzite cave is dominated by the Chloroflexi (Class Ktedonobacterales) and Thaumarchaeota Group I.1c.

    abstract::The majority of caves are formed within limestone rock and hence our understanding of cave microbiology comes from carbonate-buffered systems. In this paper, we describe the microbial diversity of Roraima Sur Cave (RSC), an orthoquartzite (SiO4) cave within Roraima Tepui, Venezuela. The cave contains a high level of m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00615

    authors: Barton HA,Giarrizzo JG,Suarez P,Robertson CE,Broering MJ,Banks ED,Vaishampayan PA,Venkateswaran K

    更新日期:2014-11-26 00:00:00

  • Exopolysaccharides From Streptococcus thermophilus ST538 Modulate the Antiviral Innate Immune Response in Porcine Intestinal Epitheliocytes.

    abstract::It was reported that exopolysaccharides (EPSs) from lactobacilli are able to differentially modulate mucosal antiviral immunity. Although research has described the ability of EPSs derived from Streptococcus thermophilus to modulate the mucosal immune system, their impact on antiviral immunity was less explored. In th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00894

    authors: Mizuno H,Tomotsune K,Islam MA,Funabashi R,Albarracin L,Ikeda-Ohtsubo W,Aso H,Takahashi H,Kimura K,Villena J,Sasaki Y,Kitazawa H

    更新日期:2020-05-19 00:00:00

  • Actin and DNA Protect Histones from Degradation by Bacterial Proteases but Inhibit Their Antimicrobial Activity.

    abstract::Histones are small polycationic proteins located in the cell nucleus. Together, DNA and histones are integral constituents of the nucleosomes. Upon apoptosis, necrosis, and infection - induced cell death, histones are released from the cell. The extracellular histones have strong antimicrobial activity but are also cy...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01248

    authors: Sol A,Skvirsky Y,Blotnick E,Bachrach G,Muhlrad A

    更新日期:2016-08-09 00:00:00

  • Clustering of Subgingival Microbiota Reveals Microbial Disease Ecotypes Associated with Clinical Stages of Periodontitis in a Cross-Sectional Study.

    abstract::Periodontitis is characterized by chronic inflammation associated with alteration of the oral microbiota. In contrast to previous microbiome studies focusing a priori on comparison between extreme phenotypes, our study analyzed a random sample of 85 people. The aim of this study was to link microbial differences to di...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00340

    authors: Boutin S,Hagenfeld D,Zimmermann H,El Sayed N,Höpker T,Greiser HK,Becher H,Kim TS,Dalpke AH

    更新日期:2017-03-01 00:00:00

  • Community Structure in Methanogenic Enrichments Provides Insight into Syntrophic Interactions in Hydrocarbon-Impacted Environments.

    abstract::The methanogenic biodegradation of crude oil involves the conversion of hydrocarbons to methanogenic substrates by syntrophic bacteria and subsequent methane production by methanogens. Assessing the metabolic roles played by various microbial species in syntrophic communities remains a challenge, but such information ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00562

    authors: Fowler SJ,Toth CR,Gieg LM

    更新日期:2016-04-22 00:00:00

  • Comparative Analysis of Genetic Determinants Encoding Cadmium, Arsenic, and Benzalkonium Chloride Resistance in Listeria monocytogenes of Human, Food, and Environmental Origin.

    abstract::Environmental adaptation of Listeria monocytogenes is a complex process involving various mechanisms that can contribute to their survival in the environment, further spreading throughout the food chain and the development of listeriosis. The aim of this study was to analyze whole-genome sequencing data in a set of 27...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.599882

    authors: Gelbicova T,Florianova M,Hluchanova L,Kalova A,Korena K,Strakova N,Karpiskova R

    更新日期:2021-01-14 00:00:00

  • The dynamic nature and territory of transcriptional machinery in the bacterial chromosome.

    abstract::Our knowledge of the regulation of genes involved in bacterial growth and stress responses is extensive; however, we have only recently begun to understand how environmental cues influence the dynamic, three-dimensional distribution of RNA polymerase (RNAP) in Escherichia coli on the level of single cell, using wide-f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00497

    authors: Jin DJ,Cagliero C,Martin CM,Izard J,Zhou YN

    更新日期:2015-05-21 00:00:00

  • Warming, but Not Acidification, Restructures Epibacterial Communities of the Baltic Macroalga Fucus vesiculosus With Seasonal Variability.

    abstract::Due to ocean acidification and global warming, surface seawater of the western Baltic Sea is expected to reach an average of ∼1100 μatm pCO2 and an increase of ∼5°C by the year 2100. In four consecutive experiments (spanning 10-11 weeks each) in all seasons within 1 year, the abiotic factors temperature (+5°C above in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01471

    authors: Mensch B,Neulinger SC,Künzel S,Wahl M,Schmitz RA

    更新日期:2020-06-26 00:00:00

  • Chromium Hyper-Tolerant Bacillus sp. MH778713 Assists Phytoremediation of Heavy Metals by Mesquite Trees (Prosopis laevigata).

    abstract::Heavy metal accumulation in mesquite trees (Prosopis laevigata) growing in aluminum, titanium, chromium and zirconium-polluted soils of a semi-arid region in Mexico was investigated using wavelength dispersive X-ray fluorescence analysis. The results showed that P. laevigata trees can hyper accumulate up to 4100 mg/kg...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01833

    authors: Ramírez V,Baez A,López P,Bustillos R,Villalobos MÁ,Carreño R,Contreras JL,Muñoz-Rojas J,Fuentes LE,Martínez J,Munive JA

    更新日期:2019-08-13 00:00:00

  • Transcriptome Profile of Yeast Strain Used for Biological Wine Aging Revealed Dynamic Changes of Gene Expression in Course of Flor Development.

    abstract::Flor strains of Saccharomyces cerevisiae are principal microbial agents responsible for biological wine aging used for production of sherry-like wines. The flor yeast velum formed on the surface of fortified fermented must is a major adaptive and technological characteristic of flor yeasts that helps them to withstand...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00538

    authors: Mardanov AV,Eldarov MA,Beletsky AV,Tanashchuk TN,Kishkovskaya SA,Ravin NV

    更新日期:2020-04-03 00:00:00

  • Expanding genomics of mycorrhizal symbiosis.

    abstract::The mycorrhizal symbiosis between soil fungi and plant roots is a ubiquitous mutualism that plays key roles in plant nutrition, soil health, and carbon cycling. The symbiosis evolved repeatedly and independently as multiple morphotypes [e.g., arbuscular mycorrhizae (AM), ectomycorrhizal (ECM)] in multiple fungal clade...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00582

    authors: Kuo A,Kohler A,Martin FM,Grigoriev IV

    更新日期:2014-11-04 00:00:00

  • Genomic Characterization of Sulphite Reducing Bacteria Isolated From the Dairy Production Chain.

    abstract::Anaerobic sporeformers, specifically spoilage and pathogenic members of the genus Clostridium, are a concern for producers of dairy products, and of powdered dairy products in particular. As an alternative to testing for individual species, the traditional, and still current, approach to detecting these sporeformers, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01507

    authors: Doyle CJ,O'Toole PW,Cotter PD

    更新日期:2018-07-05 00:00:00

  • Sterilization of liquid foods by pulsed electric fields-an innovative ultra-high temperature process.

    abstract::The intention of this study was to investigate the inactivation of endospores by a combined thermal and pulsed electric field (PEF) treatment. Therefore, self-cultivated spores of Bacillus subtilis and commercial Geobacillus stearothermophilus spores with certified heat resistance were utilized. Spores of both strains...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00400

    authors: Reineke K,Schottroff F,Meneses N,Knorr D

    更新日期:2015-05-06 00:00:00

  • Nuclear factor kappa B is required for the production of infectious human herpesvirus 8 virions.

    abstract::Human herpesvirus 8 (HHV8) infection leads to potent activation of nuclear factor kappa B (NFκB) in primary and transformed cells. We used recombinant HHV8 (rKSHV.219) expressing green fluorescent protein under the constitutive cellular promoter elongation factor 2α and red fluorescent protein under an early HHV8 lyti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00129

    authors: Blattman NN,Lagunoff M,Blattman JN,Corey L

    更新日期:2014-04-04 00:00:00