Unraveling the Microbial Interactions and Metabolic Potentials in Pre- and Post-treated Sludge from a Wastewater Treatment Plant Using Metagenomic Studies.

Abstract:

:Sewage waste represents an ecosystem of complex and interactive microbial consortia which proliferate with different kinetics according to their individual genetic as well as metabolic potential. We performed metagenomic shotgun sequencing on Ion-Torrent platform, to explore the microbial community structure, their biological interactions and associated functional capacity of pre-treated/raw sludge (RS) and post-treated/dried sludge (DS) of wastewater treatment plant. Bacterial phylotypes belonging to Epsilonproteobacteria (∼45.80%) dominated the RS with relatively few Archaea (∼1.94%) whereas DS has the dominance of beta- (30.23%) and delta- (13.38%) classes of Proteobacteria with relatively greater abundance of Archaea (∼7.18%). In particular, Epsilonproteobacteria appears as a primary energy source in RS and sulfur-reducing bacteria with methanogens seems to be in the potential syntrophic association in DS. These interactions could be ultimately responsible for carrying out amino-acid degradation, aromatic compound degradation and degradation of propionate and butyrate in DS. Our data also reveal the presence of key genes in the sludge microbial community responsible for degradation of polycyclic aromatic hydrocarbons. Potential pathogenic microbes and genes for the virulence factors were found to be relatively abundant in RS which clearly reflect the necessity of treatment of RS. After treatment, potential pathogens load was reduced, indicating the sludge hygienisation in DS. Additionally, the interactions found in this study would reveal the biological and environmental cooperation among microbial communities for domestic wastewater treatment.

journal_name

Front Microbiol

authors

Sidhu C,Vikram S,Pinnaka AK

doi

10.3389/fmicb.2017.01382

subject

Has Abstract

pub_date

2017-07-19 00:00:00

pages

1382

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Hexokinase and Glucokinases Are Essential for Fitness and Virulence in the Pathogenic Yeast Candida albicans.

    abstract::The pathogenic yeast Candida albicans is both a powerful commensal and a pathogen of humans that can infect wide range of organs and body sites. Metabolic flexibility promotes infection and commensal colonization by this opportunistic pathogen. Yeast cell survival depends upon assimilation of fermentable and non-ferme...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00327

    authors: Laurian R,Dementhon K,Doumèche B,Soulard A,Noel T,Lemaire M,Cotton P

    更新日期:2019-02-25 00:00:00

  • Diversity and Expression of Bacterial Metacaspases in an Aquatic Ecosystem.

    abstract::Metacaspases are distant homologs of metazoan caspase proteases, implicated in stress response, and programmed cell death (PCD) in bacteria and phytoplankton. While the few previous studies on metacaspases have relied on cultured organisms and sequenced genomes, no studies have focused on metacaspases in a natural set...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01043

    authors: Asplund-Samuelsson J,Sundh J,Dupont CL,Allen AE,McCrow JP,Celepli NA,Bergman B,Ininbergs K,Ekman M

    更新日期:2016-07-06 00:00:00

  • Comparison of Two Sampling Techniques for Evaluating Ruminal Fermentation and Microbiota in the Planktonic Phase of Rumen Digesta in Dairy Cows.

    abstract::The objective of this experiment was to compare ruminal fluid samples collected through rumen cannula (RC) or using an oral stomach tube (ST) for measurement of ruminal fermentation and microbiota variables. Six ruminally cannulated lactating Holstein cows fed a standard diet were used in the study. Rumen samples were...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618032

    authors: de Assis Lage CF,Räisänen SE,Melgar A,Nedelkov K,Chen X,Oh J,Fetter ME,Indugu N,Bender JS,Vecchiarelli B,Hennessy ML,Pitta D,Hristov AN

    更新日期:2020-12-23 00:00:00

  • The Lp_3561 and Lp_3562 Enzymes Support a Functional Divergence Process in the Lipase/Esterase Toolkit from Lactobacillus plantarum.

    abstract::Lactobacillus plantarum species is a good source of esterases since both lipolytic and esterase activities have been described for strains of this species. No fundamental biochemical difference exists among esterases and lipases since both share a common catalytic mechanism. L. plantarum WCFS1 possesses a protein, Lp_...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01118

    authors: Esteban-Torres M,Reverón I,Santamaría L,Mancheño JM,de Las Rivas B,Muñoz R

    更新日期:2016-07-19 00:00:00

  • Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China.

    abstract::Incompatibility group C (IncC) plasmids have received attention due to their broad host range and because they harbor key antibiotic resistance genes. Because these resistance genes can spread from food-producing animals to human, the proliferation of these plasmids represents a public health risk. In this study, a to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.580960

    authors: Zhang Y,Lei CW,Chen X,Yao TG,Yu JW,Hu WL,Mao X,Wang HN

    更新日期:2020-10-27 00:00:00

  • An RNA Virome Associated to the Golden Orb-Weaver Spider Nephila clavipes.

    abstract::The golden orb-weaver spider Nephila clavipes, known for its sexual size dimorphism, is abundant and widespread in the New World. The first annotated genome of orb-weaver spiders, exploring N. clavipes, has recently been reported. The study, focused primarily on the diversity of silk specific genes, shed light into th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02097

    authors: Debat HJ

    更新日期:2017-10-25 00:00:00

  • The Russian Doll Model: How Bacteria Shape Successful and Sustainable Inter-Kingdom Relationships.

    abstract::Successful inter-kingdom relationships are based upon a dynamic balance between defense and cooperation. A certain degree of competition is necessary to guarantee life spread and development. On the other hand, cooperation is a powerful tool to ensure a long lasting adaptation to changing environmental conditions and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.573759

    authors: Pessione E

    更新日期:2020-10-20 00:00:00

  • Insights Into the Evolution of Staphylococcus aureus Daptomycin Resistance From an in vitro Bioreactor Model.

    abstract::The extensive use of daptomycin for treating complex methicillin-resistant Staphylococcus aureus infections has led to the emergence of daptomycin-resistant strains. Although genomic studies have identified mutations associated with daptomycin resistance, they have not necessarily provided insight into the evolution a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00345

    authors: Lasek-Nesselquist E,Lu J,Schneider R,Ma Z,Russo V,Mishra S,Pai MP,Pata JD,McDonough KA,Malik M

    更新日期:2019-02-28 00:00:00

  • Temporal changes in the diazotrophic bacterial communities associated with Caribbean sponges Ircinia stroblina and Mycale laxissima.

    abstract::Sponges that harbor microalgal or, cyanobacterial symbionts may benefit from photosynthetically derived carbohydrates, which are rich in carbon but devoid of nitrogen, and may therefore encounter nitrogen limitation. Diazotrophic communities associated with two Caribbean sponges, Ircinia strobilina and Mycale laxissim...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00561

    authors: Zhang F,Vicente J,Hill RT

    更新日期:2014-10-28 00:00:00

  • Microbial communities and chemosynthesis in yellowstone lake sublacustrine hydrothermal vent waters.

    abstract::Five sublacustrine thermal spring locations from 1 to 109 m water depth in Yellowstone Lake were surveyed by 16S ribosomal RNA gene sequencing in relation to their chemical composition and dark CO(2) fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and elec...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00130

    authors: Yang T,Lyons S,Aguilar C,Cuhel R,Teske A

    更新日期:2011-06-13 00:00:00

  • Automated quantification of the phagocytosis of Aspergillus fumigatus conidia by a novel image analysis algorithm.

    abstract::Studying the pathobiology of the fungus Aspergillus fumigatus has gained a lot of attention in recent years. This is due to the fact that this fungus is a human pathogen that can cause severe diseases, like invasive pulmonary aspergillosis in immunocompromised patients. Because alveolar macrophages belong to the first...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00549

    authors: Kraibooj K,Schoeler H,Svensson CM,Brakhage AA,Figge MT

    更新日期:2015-06-09 00:00:00

  • Exploring the Genomic Traits of Non-toxigenic Vibrio parahaemolyticus Strains Isolated in Southern Chile.

    abstract::Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis worldwide. As reported in other countries, after the rise and fall of the pandemic strain in Chile, other post-pandemic strains have been associated with clinical cases, including strains lacking the major toxins TDH and TRH. Since the prese...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00161

    authors: Castillo D,Pérez-Reytor D,Plaza N,Ramírez-Araya S,Blondel CJ,Corsini G,Bastías R,Loyola DE,Jaña V,Pavez L,García K

    更新日期:2018-02-08 00:00:00

  • Systemic effector and regulatory immune responses to chlamydial antigens in trachomatous trichiasis.

    abstract::Trachomatous trichiasis (TT) caused by repeated or chronic ocular infection with Chlamydia trachomatis is the result of a pro-fibrotic ocular immune response. At the conjunctiva, the increased expression of both inflammatory (IL1B, TNF) and regulatory cytokines (IL10) have been associated with adverse clinical outcome...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00010

    authors: Gall A,Horowitz A,Joof H,Natividad A,Tetteh K,Riley E,Bailey RL,Mabey DC,Holland MJ

    更新日期:2011-02-10 00:00:00

  • A Mixed Infection of Helenium Virus S With Two Distinct Isolates of Butterbur Mosaic Virus, One of Which Has a Major Deletion in an Essential Gene.

    abstract::Multiple carlaviruses infect various ornamental plants, often having limited host ranges and causing minor symptoms, yet often reducing yield or quality. In this study we have identified a mixed infection of butterbur mosaic virus (ButMV) and helenium virus S (HelVS) from a plant of veronica (Veronica sp.) showing fol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.612936

    authors: Hammond J,Reinsel M,Grinstead S,Lockhart B,Jordan R,Mollov D

    更新日期:2020-12-21 00:00:00

  • Transcriptional Approach for Decoding the Mechanism of rpoC Compensatory Mutations for the Fitness Cost in Rifampicin-Resistant Mycobacterium tuberculosis.

    abstract::Multidrug-resistant tuberculosis (TB), defined as TB resistant to the two first-line drugs, isoniazid and rifampin, is a serious challenge to global TB eradication efforts. Although mutations in rpoA or rpoC have been proposed to compensate for this fitness cost due to rpoB mutation in rifampicin-resistant Mycobacteri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02895

    authors: Xu Z,Zhou A,Wu J,Zhou A,Li J,Zhang S,Wu W,Karakousis PC,Yao YF

    更新日期:2018-11-30 00:00:00

  • Malaria and the liver: immunological hide-and-seek or subversion of immunity from within?

    abstract::During the pre-erythrocytic asymptomatic phase of malarial infection, sporozoites develop transiently inside less than 100 hepatocytes that subsequently release thousands of merozoites. Killing of these hepatocytes by cytotoxic T cells (CTLs) confers protection to subsequent malarial infection, suggesting that this bo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00041

    authors: Bertolino P,Bowen DG

    更新日期:2015-02-18 00:00:00

  • Microbial Community Structure and the Persistence of Cyanobacterial Populations in Salt Crusts of the Hyperarid Atacama Desert from Genome-Resolved Metagenomics.

    abstract::Although once thought to be devoid of biology, recent studies have identified salt deposits as oases for life in the hyperarid Atacama Desert. To examine spatial patterns of microbial species and key nutrient sources, we genomically characterized 26 salt crusts from three sites along a fog gradient. The communities ar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01435

    authors: Finstad KM,Probst AJ,Thomas BC,Andersen GL,Demergasso C,Echeverría A,Amundson RG,Banfield JF

    更新日期:2017-07-28 00:00:00

  • Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris.

    abstract::Pichia pastoris (Komagataella sp.) is broadly used for the production of secreted recombinant proteins. Due to the high rate of protein production, incorrectly folded proteins may accumulate in the endoplasmic reticulum (ER). To restore their proper folding, the cell triggers the unfolded protein response (UPR); howev...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00335

    authors: Raschmanová H,Zamora I,Borčinová M,Meier P,Weninger A,Mächler D,Glieder A,Melzoch K,Knejzlík Z,Kovar K

    更新日期:2019-02-27 00:00:00

  • A Homeobox Transcription Factor UvHOX2 Regulates Chlamydospore Formation, Conidiogenesis, and Pathogenicity in Ustilaginoidea virens.

    abstract::Rice false smut fungus (teleomorph: Villosiclava virens; anamorph: Ustilaginoidea virens) can generate chlamydospores and survive winter under field conditions. The chlamydospore is considered as an important infection source of the disease. However, little is known about the regulatory mechanism of the chlamydospore ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01071

    authors: Yu J,Yu M,Song T,Cao H,Pan X,Yong M,Qi Z,Du Y,Zhang R,Yin X,Liu Y

    更新日期:2019-06-20 00:00:00

  • Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence P

    abstract::The genus Macrococcus is a close relative of the genus Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from human clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered from human clinical material and identified as Mac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01178

    authors: Mašlaňová I,Wertheimer Z,Sedláček I,Švec P,Indráková A,Kovařovic V,Schumann P,Spröer C,Králová S,Šedo O,Krištofová L,Vrbovská V,Füzik T,Petráš P,Zdráhal Z,Ružičková V,Doškař J,Pantuček R

    更新日期:2018-06-13 00:00:00

  • Biophysical features of MagA expression in mammalian cells: implications for MRI contrast.

    abstract::We compared overexpression of the magnetotactic bacterial gene MagA with the modified mammalian ferritin genes HF + LF, in which both heavy and light subunits lack iron response elements. Whereas both expression systems have been proposed for use in non-invasive, magnetic resonance (MR) reporter gene expression, limit...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00029

    authors: Sengupta A,Quiaoit K,Thompson RT,Prato FS,Gelman N,Goldhawk DE

    更新日期:2014-02-05 00:00:00

  • Corrigendum: Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis.

    abstract::[This corrects the article on p. 37 in vol. 6, PMID: 25699029.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2015.00538

    authors: Ungerfeld EM

    更新日期:2015-06-02 00:00:00

  • Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity.

    abstract::Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether pol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01695

    authors: Xu X,Cui X,Zhu L,Li Z,Zhang Y,Ma L,Pan W

    更新日期:2018-07-25 00:00:00

  • The Fungus Aspergillus aculeatus Enhances Salt-Stress Tolerance, Metabolite Accumulation, and Improves Forage Quality in Perennial Ryegrass.

    abstract::Perennial ryegrass (Lolium perenne) is an important forage grass with high yield and superior quality in temperate regions which is widely used in parks, sport field, and other places. However, perennial ryegrass is moderately tolerant to salinity stress compared to other commercial cultivars and salt stress reduces t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01664

    authors: Li X,Han S,Wang G,Liu X,Amombo E,Xie Y,Fu J

    更新日期:2017-09-04 00:00:00

  • Regulatory RNAs in the Less Studied Streptococcal Species: From Nomenclature to Identification.

    abstract::Streptococcal species are Gram-positive bacteria involved in severe and invasive diseases in humans and animals. Although, this group includes different pathogenic species involved in life-threatening infections for humans, it also includes beneficial species, such as Streptococcus thermophilus, which is used in yogur...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01161

    authors: Zorgani MA,Quentin R,Lartigue MF

    更新日期:2016-07-26 00:00:00

  • Plant and Microbial Responses to Repeated Cu(OH)2 Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.

    abstract::The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realisti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01769

    authors: Simonin M,Colman BP,Tang W,Judy JD,Anderson SM,Bergemann CM,Rocca JD,Unrine JM,Cassar N,Bernhardt ES

    更新日期:2018-07-31 00:00:00

  • Characterization and Bio-Typing of Multidrug Resistance Plasmids From Uropathogenic Escherichia coli Isolated From Clinical Setting.

    abstract::Urinary tract infection is primarily caused by Escherichia coli. Multidrug resistance and their rapid dissemination in this pathogenic microbe complicate therapeutic strategies and threaten public health. Conjugation systems responsible for interbacterial transmission of antibiotic resistance are plasmid-encoded and c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02913

    authors: Mukherjee SK,Mukherjee M

    更新日期:2019-12-18 00:00:00

  • Diversity and Temporal Dynamics of the Epiphytic Bacterial Communities Associated with the Canopy-Forming Seaweed Cystoseira compressa (Esper) Gerloff and Nizamuddin.

    abstract::Canopy-forming seaweed species of the genus Cystoseira form diverse and productive habitats along temperate rocky coasts of the Mediterranean Sea. Despite numerous studies on the rich macrofauna and flora associated with Cystoseira spp., there is little knowledge about the epiphytic bacteria. We analyzed bacterial pop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00476

    authors: Mancuso FP,D'Hondt S,Willems A,Airoldi L,De Clerck O

    更新日期:2016-04-08 00:00:00

  • Transcriptome Analysis Reveals the Flexibility of Cordycepin Network in Cordyceps militaris Activated by L-Alanine Addition.

    abstract::Cordycepin, isolated from the traditional medicinal fungus Cordyceps militaris, has gained much attention due to its various clinical functions. Previous reports of L-alanine addition could significantly improve cordycepin production, but the molecular mechanism remains unknown. In this study, transcriptome analysis o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00577

    authors: Chen BX,Wei T,Xue LN,Zheng QW,Ye ZW,Zou Y,Yang Y,Yun F,Guo LQ,Lin JF

    更新日期:2020-04-24 00:00:00

  • Green Tea Catechin-Inactivated Viral Vaccine Platform.

    abstract::Traditionally, chemical agents such as formalin (FA) and β-propiolactone (BPL) have long been used for the preparation of inactivated vaccines or toxoids. It has been shown that FA extensively modifies vaccine antigens and thus affects immunogenicity profiles, sometimes compromising the protective efficacy of the vacc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02469

    authors: Lee YH,Jang YH,Byun YH,Cheong Y,Kim P,Lee YJ,Lee YJ,Sung JM,Son A,Lee HM,Lee J,Yang SW,Song JM,Seong BL

    更新日期:2017-12-12 00:00:00