Abstract：
:Chimeric DNA vaccines co-expressing Eimeria acervulina macrophage migration inhibitory factor (E.MIF) and chicken IL-2 (IL-2) or interferon-γ (IFN-γ) were constructed and their efficacies against E. acervulina were evaluated. The open reading frame (ORF) of E.MIF was cloned from E. acervulina merozoites and subcloned into the eukaryotic expression vector pVAX1 with chicken cytokine gene IFN-γ or IL-2 to construct the DNA vaccines pVAX-E.MIF-IFN-γ, pVAX-E.MIF-IL-2 and pVAX-E.MIF. The in vivo transfection of the target genes was detected by use of reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Immunizations were carried out by vaccinating chickens twice with a dose rate of 100 μg intramuscularly. Seven days post second immunization, all chickens except the unchallenged control group were challenged orally with 1 × 105 sporulated oocysts of E. acervulina. Seven days later, the duodenum was collected. The results showed that the target genes were expressed effectively in vivo. DNA vaccines and the recombinant E.MIF protein could alleviate body weight loss and duodenal lesions significantly compared to the control groups. Furthermore, pVAX-E.MIF-IL-2 and pVAX-E.MIF-IFN-γ induced anticoccidial indexs (ACIs) of 179.12 and 170, respectively, which were significantly higher than that of pVAX-E.MIF (ACI = 162.31). Our results demonstrated that E.MIF is a potential vaccine candidate against E. acervulina and chicken IFN-γ or IL- 2 may be used as genetic adjuvants to improve the efficacies of DNA vaccines against avian coccidiosis.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Song X,Zhang R,Xu L,Yan R,Li Xdoi
10.1515/ap-2015-0071subject
Has Abstractpub_date
2015-09-01 00:00:00pages
500-8issue
3eissn
1230-2821issn
1896-1851pii
/j/ap.2015.60.issue-3/ap-2015-0071/ap-2015-0071.xmjournal_volume
60pub_type
杂志文章abstract：BACKGROUND:Giardia duodenalis is a widespread protozoan parasite affecting humans and many species of animals, including dogs and cats. Due to its zoonotic potential, it is important to know the frequency of this parasite in companion animals. The aim of this study was to determine current epidemiological status of G. ...
journal_title：Acta parasitologica
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doi：10.1007/s11686-020-00292-1
更新日期：2020-10-11 00:00:00
abstract：:A new species of Coronocestus Caira, Marques, Jensen, Kuchta and Ivanov, 2013 is described from Iago omanensis (Norman) from the Gulf of Oman. Coronocestus ehsanentezarii sp. nov. differs from C. musteli (Pintner, 1889) Caira, Marques, Jensen, Kuchta and Ivanov, 2013 in that its ovary is H- rather than U-shape. The ne...
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abstract：:A new species of Rhinonastes, hitherto monotypic, is described and illustrated from the nasal cavity of 'curimat', Prochilodus argenteus from São Francisco River, Brazil. Rhinonastes curimatae n. sp. presents a male copulatory organ with more than five rings while the type species of the genus is characterized by male...
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更新日期：2014-09-01 00:00:00
abstract：:PURPOSE : The present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. We also determined cytotoxic effect, enzymatic and non-enzymatic antioxidant activities, total phenolic, lavonoid and antioxidative co...
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更新日期：2019-03-01 00:00:00
abstract：:Rhabdias paraensis Santos, Melo, Nascimento, Nascimento, Giese et Furtado, 2011 was described based on fully gravid worms. Further investigations on the free-living stages, immature worms and young individuals were facilitated by cultivation in the laboratory, which allowed us to add new information about the morpholo...
journal_title：Acta parasitologica
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更新日期：2016-01-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2016-0064
更新日期：2016-09-01 00:00:00
abstract：:Hookworms, a group to which Ancylostoma ceylanicum belongs, are gastrointestinal nematodes that infect more than 700 million people around the world. They are a leading cause of anemia in developing countries. In order to effectively prevent hookworm infections research is conducted to develop an effective vaccine usi...
journal_title：Acta parasitologica
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更新日期：2016-09-01 00:00:00
abstract：:The aim of this retrospective, population-based study was to characterize demographically and clinically cystic-echinococcosis (CE) in southern Israel, between 2005 and 2012. Newly-diagnosed (nd-CE) and past-diagnosed (pd-CE, diagnosed before the study) cases were defined. Two populations live in southern-Israel, rece...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2016-0024
更新日期：2016-01-01 00:00:00
abstract：:Parasitization of silkworm, Bombyx mori by invasive larva of dipteran parasitoid Exorista bombycis caused upto 20% revenue loss in sericulture. The parasitism was successful by suppressing host immune system however mechanism of immune suppression induced by E. bombycis is unknown which is unravelled here. The infesta...
journal_title：Acta parasitologica
pub_type： 杂志文章
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更新日期：2017-12-20 00:00:00
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pub_type： 杂志文章
doi：10.2478/s11686-014-0234-2
更新日期：2014-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1515/ap-2016-0114
更新日期：2016-12-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00108-x
更新日期：2019-12-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2016-0084
更新日期：2016-09-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-013-0116-z
更新日期：2013-03-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2017-0025
更新日期：2017-03-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0209-3
更新日期：2014-03-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-020-00219-w
更新日期：2020-12-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
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更新日期：2017-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11686-020-00281-4
更新日期：2020-09-15 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2018-0035
更新日期：2018-06-26 00:00:00
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pub_type： 杂志文章
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更新日期：2018-09-25 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-020-00170-w
更新日期：2020-06-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2015-0033
更新日期：2015-06-01 00:00:00
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journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-013-0103-4
更新日期：2013-03-01 00:00:00
abstract：:The Mascarene grass frog Ptychadena cf. mascareniensis is a species of frog with a vast area of distribution in Africa. A total of 300 frog specimens were collected from different localities at El-Giza province, Egypt; then dissected and examined for the presence of parasitic infection. Only eighty six (28.66%) specim...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2017-0052
更新日期：2017-06-01 00:00:00
abstract：:Aspiculuris tianjinensis sp. nov. recovered from the intestine of Clethrionomys rufocanus from Tianjin, China is described and illustrated using light microscopy and scanning electron microscopy. The new species differs from congeners in the shape of the cervical alae, and the number and arrangement of caudal papillae...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-012-0031-8
更新日期：2012-09-01 00:00:00
abstract：PURPOSE:Fish parasites can cause diseases in humans and lead to commercial losses in fisheries and aquaculture. The objectives of this study were to analyze E. ongus's parasite fauna regarding food safety and parasite transmission risk between Epinephelus species and test whether E.ongus populations can be distinguishe...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1007/s11686-020-00312-0
更新日期：2020-12-14 00:00:00
abstract：:Felids are the only definitive hosts of Toxoplasma gondii. To lay a foundation for screening the T. gondii-felids interaction factors, we have developed a reproducible primary culture method for cat intestinal epithelial cells (IECs). The primary IECs were isolated from a new born cat's small intestine jejunum region ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2018-0041
更新日期：2018-06-26 00:00:00
abstract：INTRODUCTION:The objective of this study was to evaluate the presence of anti-Sarcocystis spp. specific IgG antibodies in serum samples from precolostral lambs to determine the occurrence of transplacental transmission of Sarcocystis spp. in sheep. METHODS:Blood samples were collected from 80 ewes and their respective...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00114-z
更新日期：2020-03-01 00:00:00
abstract：:A parasitological investigation was performed on a total of 5380 Atlantic cod larvae, post-larvae and small juveniles sampled from the North Sea during a period of five years. The copepod Caligus elongatus (Von Nordmann, 1832) and the nematode Hysterothylacium aduncum (Rudolphi, 1802) were found at a relatively high p...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0244-0
更新日期：2014-06-01 00:00:00
