Abstract：
:Felids are the only definitive hosts of Toxoplasma gondii. To lay a foundation for screening the T. gondii-felids interaction factors, we have developed a reproducible primary culture method for cat intestinal epithelial cells (IECs). The primary IECs were isolated from a new born cat's small intestine jejunum region without food ingress, and respectively in vitro cultured by tissue cultivation and combined digestion method with collagenase XI and dispase I, then purified by trypsinization. After identification, the ds cDNA of cat IECs was synthesized for constructing pGADT7 homogenization three-frame plasmid, and transformed into the yeast Y187 for generating the cDNA library. Our results indicated that cultivation of primary cat IECs relays on combined digestion to form polarized and confluent monolayers within 3 days with typical features of normal epithelial cells. The purified cells cultured by digestion method were identified to be nature intestinal epithelial cells using immunohistochemical analysis and were able to maintain viability for at least 15 passages. The homogenizable ds cDNA, which is synthesized from the total RNA extracted from our cultured IECs, distributed among 0.5-2.0 kb, and generated satisfying three-frame cDNA library with the capacity of 1.2 × 106 and the titer of 5.2 × 107 pfu/mL. Our results established an optimal method for the culturing and passage of cat IECs model in vitro, and laid a cDNA library foundation for the subsequent interaction factors screening by yeast two-hybrid.
journal_name
Acta Parasitoljournal_title
Acta parasitologicaauthors
Zhao GH,Liu Y,Cheng YT,Zhao QS,Qiu X,Xu C,Xiao T,Zhu S,Liu GZ,Yin Kdoi
10.1515/ap-2018-0041subject
Has Abstractpub_date
2018-06-26 00:00:00pages
360-367issue
2eissn
1230-2821issn
1896-1851pii
/j/ap.2018.63.issue-2/ap-2018-0041/ap-2018-0041.xmjournal_volume
63pub_type
杂志文章abstract：:The Mascarene grass frog Ptychadena cf. mascareniensis is a species of frog with a vast area of distribution in Africa. A total of 300 frog specimens were collected from different localities at El-Giza province, Egypt; then dissected and examined for the presence of parasitic infection. Only eighty six (28.66%) specim...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2017-0052
更新日期：2017-06-01 00:00:00
abstract：:The ultrastructure of gamonts and sporulated oocysts of Goussia metchnikovi in the spleen of gudgeon, Gobio gobio from the river Lee, England is described. In developing microgamonts, small amylopectin granules were grouped centrally and nuclei were often arranged peripherally, close to the surface membrane. Nuclear c...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-012-0014-9
更新日期：2012-03-01 00:00:00
abstract：:This paper characterizes the pattern of ectoparasite and endoparasite communities in an assemblage of 35 sympatric fish from different trophic levels in a tributary from the Amazon River system, northern Brazil. In detritivorous, carnivorous, omnivorous and piscivorous hosts, the species richness consisted of 82 ectop...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2018-0035
更新日期：2018-06-26 00:00:00
abstract：:Maritrema formicae sp. nov. is described from the Patagonian coast, Argentina, based on adults obtained from the kelp gull, Larus dominicanus. The new species fits with the "eroliae complex" and can be distinguished from other related species mainly in shape and size of body, shape, size, and pattern of distribution o...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-012-0019-4
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:In spite of the global economic significance of sheep production, little is known about the prevalence of various Sarcocystis spp. infecting the domestic sheep (Ovis aries) in Egypt. MATERIALS AND METHODS:Muscle samples were collected from 175 sheep (> 2 years) slaughtered at El-Mahalla El-Kubra abattoir, G...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00070-8
更新日期：2019-09-01 00:00:00
abstract：:Sera collected from healthy individuals from the general population in the Czech Republic during repeated cross-sectional surveys were analyzed. Samples collected in the same six districts in two time periods, 1978-1989 and 2001, were compared. The study subjects were divided into six age categories between 10 and 59 ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2018-0004
更新日期：2018-03-26 00:00:00
abstract：INTRODUCTION:Entomopathogenic nematodes (EPNs) are important biocontrol agents of insect pests. To increase the availability of locally adapted entomopathogenic nematode isolates for biocontrol programs, a survey of several agricultural soils in Western Uttar Pradesh, India was conducted. MATERIALS AND METHODS:Eight h...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1007/s11686-020-00279-y
更新日期：2020-09-24 00:00:00
abstract：PURPOSE:To describe a new genus and a new species of an ectoparasitic ergasilid (Copepoda, Ergasilidae) parasite of the red-tailed lambari, Astyanax fasciatus, from Jurumirim Reservoir (Upper Paranapanema River), São Paulo State, Brazil. METHODS:The host fish were collected using multi-panel gill nets. The gill of eac...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00108-x
更新日期：2019-12-01 00:00:00
abstract：PURPOSE:Due to mass population movements driven by internal conflicts and wars, cutaneous leishmaniasis (CL) is becoming increasingly important in Turkey. This study is aimed at determining the clinical aspects, diagnosis and genotyping of CL patients coming to Turkey from abroad. METHODS:In our study, the clinical ma...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00139-4
更新日期：2020-03-01 00:00:00
abstract：INTRODUCTION:The grey wolf (Canis lupus) is the natural host of many parasites. These animals travel quite long distances to search for prey and nests, causing parasites to spread over large areas; therefore, determination of the parasites carried by grey wolves is important. METHODS:In this study, we used both morpho...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1007/s11686-020-00311-1
更新日期：2020-11-22 00:00:00
abstract：:Perumytilus purpuratus is an abundant bivalve located in the intertidal rocky zone of South America that has been considered as a key species of the ecosystem. There are few studies of the host-parasite relationship of this bivalve; thus, this research aims to analyse the spatial and temporal variation in the prevalen...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-013-0125-y
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND:Giardia duodenalis is a widespread protozoan parasite affecting humans and many species of animals, including dogs and cats. Due to its zoonotic potential, it is important to know the frequency of this parasite in companion animals. The aim of this study was to determine current epidemiological status of G. ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1007/s11686-020-00292-1
更新日期：2020-10-11 00:00:00
abstract：PURPOSE:Sri Lanka is endemic to cutaneous leishmaniasis and reported as the latest focus of leishmaniasis in the Asian subcontinent. Annually, the number of leishmaniasis cases is increasing; therefore, more efficient diagnostic tools, treatment methods and effective prevention measures are indispensable. For this reas...
journal_title：Acta parasitologica
pub_type： 杂志文章,评审
doi：10.2478/s11686-020-00174-6
更新日期：2020-06-01 00:00:00
abstract：:Cosmocerca leytensis sp. nov. (Ascaridida, Cosmocercidae) from the large intestine of Cyrtodactylus gubaot (Squamata: Gekkonidae) collected on Leyte Island, Philippines is described and illustrated. Cosmocerca leytensis sp. nov. is the 30th species assigned to the genus, the 4th from the Oriental region, and the first...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2015-0096
更新日期：2015-12-01 00:00:00
abstract：:Trypanosoma cruzi (Tc), the causative agent of Chagas disease, affects millions of people worldwide. One of the major characteristics of T. cruzi is related to its heterogeneity due to the variability of its biological properties, parasite growth rates, infectivity, tissue tropism, morbidity and virulence among differ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2015-0060
更新日期：2015-09-01 00:00:00
abstract：BACKGROUND:Parasitism is a complex problem that is often ignored in companion animals, including birds, unless it develops into a severe clinical disorder. The present study was, therefore, aimed to investigate the presence of the gastrointestinal nematode infecting the domestic pigeon and provide a complete morphologi...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00151-8
更新日期：2020-03-01 00:00:00
abstract：UNLABELLED:Geographic information system (GIS) or Health geography is a geographical identification of the spatial variation of contagion disease and zoonosis risk in certain conditions. OBJECTIVE:Comparing the variation of environmental contamination of Toxocara spp. sands of lawns and public areas through the Geogra...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-013-0142-x
更新日期：2013-09-01 00:00:00
abstract：:Nematodes Ashworthius sidemi are typical parasites of Asiatic cervids, mostly sika deer (Cervus nippon). Migrating red deer have probably introduced this parasite to the area of Poland from neighbouring countries. The aim of the study was to determine prevalence and intensity of infection of red deer and roe deer from...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2017-0103
更新日期：2017-12-20 00:00:00
abstract：:The smokybrown cockroach Periplaneta fuliginosa has spread all over the world, and is now one of the most undesired invasive alien pests in Japan. Because cockroaches are generally infected by thelastomatid nematodes, they are being distributed around the world with their parasitic nematodes. Nothing is known about pa...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0230-6
更新日期：2014-06-01 00:00:00
abstract：PURPOSE:Leishmaniasis, as one of the most important vector-borne and zoonotic diseases, can be seen in different forms and is more prevalent in developing countries worldwide. Due to the absence of effective strategies in its prevention, treatment, and control, investigation of effective control strategies against the ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00068-2
更新日期：2019-09-01 00:00:00
abstract：:PURPOSE : The present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. We also determined cytotoxic effect, enzymatic and non-enzymatic antioxidant activities, total phenolic, lavonoid and antioxidative co...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-018-00009-5
更新日期：2019-03-01 00:00:00
abstract：:A parasitological investigation was performed on a total of 5380 Atlantic cod larvae, post-larvae and small juveniles sampled from the North Sea during a period of five years. The copepod Caligus elongatus (Von Nordmann, 1832) and the nematode Hysterothylacium aduncum (Rudolphi, 1802) were found at a relatively high p...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0244-0
更新日期：2014-06-01 00:00:00
abstract：:Intestinal coccidian parasites are intracellular protozoa most frequently transmitted during food-borne and water-borne infections. This group of parasites is responsible for acute diarrhoeal illnesses especially among immunocompromised patients. However, they are more frequently detected in immunocompetent individual...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2017-0077
更新日期：2017-09-26 00:00:00
abstract：:Fascioliasis is an important food-borne parasitic zoonosis caused by two trematode species, Fasciola hepatica and Fasciola gigantica. The characterisation and differentiation of Fasciola populations is crucial to control the disease, given the different transmission, epidemiology and pathology characteristics of the t...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2016-0037
更新日期：2016-03-01 00:00:00
abstract：:Polyopisthocotylean monogenean parasites of fishes are highly host specific and have been used as an appropriate model to study the host-parasite co-evolution. In the present study, eight monogeneans of the order Mazocraeidea were characterized by nuclear 28S rDNA sequences and their phylogenetic relationship with oth...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2016-0047
更新日期：2016-03-01 00:00:00
abstract：:The black grouper Mycteroperca bonaci is a commercially important fish off the coast of Yucatan, Mexico. To investigate possible differences between parasite communities in two different environments, 60 fish were collected from two areas during 2010 and 2011 and examined for parasites. The fish were classified into t...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1515/ap-2015-0067
更新日期：2015-09-01 00:00:00
abstract：PURPOSE:There are currently nine monogenoidean species of Rhabdosynochus infecting the gill lamellae of wild and cultured centropomid fishes from tropical and subtropical waters of the western Atlantic and eastern Pacific Oceans. The purpose of the present study was to describe the morphological distinctiveness of two ...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.1007/s11686-020-00303-1
更新日期：2020-11-08 00:00:00
abstract：:A new species of Rhinonastes, hitherto monotypic, is described and illustrated from the nasal cavity of 'curimat', Prochilodus argenteus from São Francisco River, Brazil. Rhinonastes curimatae n. sp. presents a male copulatory organ with more than five rings while the type species of the genus is characterized by male...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-014-0278-3
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:The aim of this study was to evaluate the prevalence and distribution of canine babesiosis and hepatozoonosis in the south of Romania as well as to assess if there are differences in the prevalence between regions in that area. METHODS:Blood samples from 300 dogs from the south of Romania were screened for the...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-020-00199-x
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Angiostrongylus cantonensis is a metastrongylid nematode that has a heteroxenous cycle, where snails act as intermediate hosts and the rodents Rattus rattus and Rattus novergicus are the definitive hosts. However, humans may act as accidental hosts presenting an atypical form of parasitism. This fact has mot...
journal_title：Acta parasitologica
pub_type： 杂志文章
doi：10.2478/s11686-019-00039-7
更新日期：2019-12-01 00:00:00
