Abstract:
:A main challenge in the development of biosensing devices for the identification and quantification of nucleic acids is to avoid the amplification of the genetic material from the sample by polymerase chain reaction (PCR), which is at present necessary to enhance sensitivity and selectivity of assays. PCR has undoubtedly revolutionized genetic analyses, but it requires careful purification procedures that are not easily implemented in point of care (POC) devices. In recent years, a new strategy for nucleic acid detection based on clustered regularly interspaced short palindromic repeats (CRISPR) and associated protein systems (Cas) seems to offer unprecedented possibilities. The coupling of the CRISPR/Cas system with recent isothermal amplification methods is fostering the development of innovative optical and electrochemical POC devices. In this review, the mechanisms of action of several new CRISRP/Cas systems are reported together with their use in biosensing of nucleic acids.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Bonini A,Poma N,Vivaldi F,Kirchhain A,Salvo P,Bottai D,Tavanti A,Di Francesco Fdoi
10.1016/j.jpba.2020.113645subject
Has Abstractpub_date
2021-01-05 00:00:00pages
113645eissn
0731-7085issn
1873-264Xpii
S0731-7085(20)31531-4journal_volume
192pub_type
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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