Abstract：
BACKGROUND:Colorectal cancer is one of the most common gastrointestinal malignancies. Photodynamic therapy (PDT) is a novel and non-invasive treatment for multiple tumors with its features of small trauma, good applicability, accurate targeting, etc. PDT is also a potential treatment for colon cancer that induces lots of suppressive effects. However, the molecular mechanism of the Chlorin e6 Photodynamic therapy (Ce6-PDT) inhibiting the migration of human colon cancer SW620 cells remains unclear. METHODS:Scratch wound healing assay, scanning electron microscope, MTT, immunofluorescence and laser confocal technique were used to investigate the suppressive effects of Ce6-PDT on the SW620 cells migration, pseudopodia, viability and the actin cytoskeleton. The effect of Ce6-PDT on actin-Filaments and signaling molecules of the Rac1/PAK1/LIMK1/cofilin signaling pathway in SW620 cells were examined by western blot analysis. RNA interference (RNAi) technology was used to establish siRNA-Rac1/SW620 cells. The combined effects of Ce6-PDT and RNAi on colon cancer SW620 cells was investigated by the same technology and methods mentioned above to clarify the signal transduction effect of Rac1/PAK1/LIMK1/cofilin signaling pathway in Ce6-PDT caused inhibition of SW620 cell migration. RESULTS:The healing and migration rate of the SW620 cells was significantly reduced and the cell pseudopodia were reduced or disappeared by Ce6-PDT. The Immunofluorescence and western blot analysis results showed that Ce6-PDT destroy microfilament's original structure and significantly downregulated F-actin protein expression. The Rac1/PAK1/LIMK1/cofilin signaling pathway was downregulated by Ce6-PDT. Furthermore, the RNAi significantly strengthened the effect of Ce6-PDT on colon cancer SW620 cells migration. CONCLUSIONS:Actin cytoskeleton and protrusions of SW620 cells correlate with its migration ability. Ce6-PDT suppresses SW620 cells migration by downregulating the Rac1/PAK1/LIMK1/cofilin signaling pathway, and its suppressive effect was enhanced by knocking down Rac1 gene expression.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Wufuer R,Ma HX,Luo MY,Xu KY,Kang Ldoi
10.1016/j.pdpdt.2020.102143subject
Has Abstractpub_date
2020-12-08 00:00:00pages
102143eissn
1572-1000issn
1873-1597pii
S1572-1000(20)30497-Xpub_type
杂志文章abstract：BACKGROUND:An appropriate photosensitizer (PS) for photodynamic inactivation should have a pronounced antimicrobial efficacy but low dark toxicity. The aim of this study is to investigate the concentration-dependent antimicrobial efficacies of methylene blue (MB) and chlorin e6 (Ce6), against Streptococcus mutans biofi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101817
更新日期：2020-09-01 00:00:00
abstract：:A case of recalcitrant dissecting cellulitis of the scalp (DCS) in a 23-year-old male patient was treated with 4 times of topical ALA-PDT at intervals of 10-15 days. The patient responded well without any unbearable adverse effects and presented satisfactory effects. Before applying this therapy, the patient and his d...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：BACKGROUND:The development of nanocarriers is an important approach to increase the bioavailability of hydrophilic drugs in target cells. In this work, we evaluated the anti-tumorigenic mechanisms and efficacy of NanoALA, a novel nanoformulation of aminolevulic acid (ALA) based on poly(lactide-co-glycolide) (PLGA) nano...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.08.011
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Considering the important roles of porphyrins in biological systems and their promising use in photodynamic therapy (PDT), the present work investigated the photophysical properties of palladium(II) 5,10,15,20-tetrakis-(4-sulfonatophenyl)-porphyrin (PdTSPP) and the effects of non-activated by light form of t...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.06.002
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:To investigate the autofluorescence (AF) characteristics of punctate inner choroidopathy (PIC) and to evaluate the progression rate of retinal pigment epithelium (RPE) atrophy lesions in PIC using confocal scanning laser ophthalmoscopy. METHODS:Twenty-two eyes of 14 PIC cases and 21 eyes of 21 non-proliferativ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.07.002
更新日期：2014-12-01 00:00:00
abstract：OBJECTIVES:To evaluate the bleaching efficacy of a violet radiation (VR) combined or not with bleaching gels on the color and mineral content of stained teeth. MATERIAL AND METHODS:Enamel/dentin blocks were obtained and stained (n = 50) with coffee, red wine, tobacco smoke, or were left non-stained. The stained or not...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2021.102194
更新日期：2021-01-26 00:00:00
abstract：:Photodynamic therapy is one of the hot topics in cancer studies. Photosensitizing chemical substrates are stimulated by light having a specific wavelength to cause fatal effect on different kinds of targets. In this study, axially 4-{[(1E)-2-furylmethylene]amino}phenol, 4-{[(1E)-2-thienylmethylene]amino}phenol and 4-{...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.05.009
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND:Vaginal intraepithelial neoplasia (VAIN) has a high-risk for recurrence and may precede genital cancers, such as vaginal cancer and/or other invasive diseases. Human papillomavirus (HPV)-induced VAIN may occur after loop electrosurgical excision procedures (LEEPs) or panhysterectomy. 5-aminolevulinic acid (A...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101837
更新日期：2020-09-01 00:00:00
abstract：:Head and neck (H&N) cancer patients have a high incidence of local field change as well as second primary lung tumours. We have applied the Wolf Diagnostic Autofluorescence Endoscopy (DAFE) system in a novel way, combining autofluorescence evaluation of both H&N region and bronchial. New H&N cases as well as "old" cas...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2006.07.004
更新日期：2006-12-01 00:00:00
abstract：BACKGROUND:Reduction of myocardial ischemia-reperfusion injury in the patients undergoing cardiac surgery under cardiopulmonary bypass represents an important goal. Intraoperative monitoring of myocardial metabolic state using continuous registration of nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucl...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.05.003
更新日期：2014-09-01 00:00:00
abstract：AIMS:To evaluate the clinical periodontal, serum glycated haemoglobin (HbA1c) and levels of advanced glycation end-products (AGEs) in the gingival crevicular fluid (GCF) among patients with periodontitis and type 2 diabetes mellitus (DM) after photodynamic therapy (PDT) as an adjunct to full-mouth disinfection (FMD). ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.pdpdt.2019.08.003
更新日期：2019-12-01 00:00:00
abstract：BACKGROUND:The gold standard treatment of early oesophageal carcinoma is oesophagectomy but the elderly population affected are often medically unfit for this radical intervention and less invasive curative options are required. We describe our experience of porfimer sodium photodynamic therapy (PDT) as an alternative ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2007.09.003
更新日期：2007-12-01 00:00:00
abstract：:In the present study, the in vitro susceptibilities of five Candida spp. to photodynamic antimicrobial chemotherapy (PACT) with four phenothiazinium derivatives, methylene blue (MB), new methylene blue N (NMBN), toluidine blue O (TBO) and the novel pentacyclic phenothiazinium photosensitiser S137, in combination with ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2012.11.004
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND:The potential application of TOOKAD(®)-PDT for the treatment of blood vessels was investigated. TOOKAD(®) (WST09), a novel palladium-bacteriopheophorbide absorbs light in the near IR with a high quantum yield of intersystem crossing. Our study assessed the efficacy of this drug in inducing vascular damage wi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(05)00006-2
更新日期：2005-03-01 00:00:00
abstract：BACKGROUND:Efficacy of daylight-photodynamic therapy (DL-PDT) with methyl aminolaevulinate (MAL) has been reported to gradually decrease as the severity of actinic keratosis (AK) lesions increases. Repeated treatments have been suggested to increase the efficacy of DL-PDT. Aim of our pilot study was to evaluate the eff...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.pdpdt.2019.05.031
更新日期：2019-09-01 00:00:00
abstract：:A phenaxazone compound [5H-Benzo[a]phenoxazin-5-one (BP)] along with an aminoquinone[2-[(o-hydroxyphenyl)amino]-1,4-naphthaquinone (HAN)] derivatives were synthesized from lawsone using ultrasound irradiation technique. The structure of the compounds were characterized by elemental analysis and various spectral studie...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.01.180
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) is a well-known and effective treatment for non-melanoma skin-cancer. Numerous studies have also shown its effectiveness in mycosis fungoides. The aim of the study was to analyse MF patients treated with PDT at the Dermatology Unit of Bologna University. METHODS:We retrospectively...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2017.09.004
更新日期：2017-12-01 00:00:00
abstract：BACKGROUND:Bowenoid papulosis is a polymorphic papular disease that occurs on the external genital area. We investigated the efficacy of 5-aminolevulinic acid-mediated photodynamic therapyin the treatment of Bowenoid papulosis. METHODS:We investigated 200 Bowenoid papulosis cases from the Department of Dermatology and...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.101635
更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:Although discovered over a century ago it is only in the last 25 years that photodynamic therapy (PDT) has been the focus of intense research. This versatile treatment modality has been shown to be effective in treating a wide variety of malignancies, and with the increasing incidence of non-melanoma skin ca...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2010.12.004
更新日期：2011-06-01 00:00:00
abstract：:Hidradenitis Suppurativa (HS) is a distressing painful chronic inflammatory skin follicular condition. It is a challenge to achieve better therapeutic effect and lower recurrence rate. In this report, ALA-PDT combined with surgery for the treatment achieved ulcer healing, pain elimination, with no relapse during our f...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.07.024
更新日期：2019-12-01 00:00:00
abstract：OBJECTIVE:To analyze the clinical efficacy of hematoporphyrin monomethyl ether photodynamic therapy (HMME-PDT) in treating port-wine stains (PWS), and its clinical operation skills and operation essentials. METHOD:A total of 16 cases of PWS with negative HMME skin test results were included in this study. Their treate...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章,评审
doi：10.1016/j.pdpdt.2017.09.013
更新日期：2017-12-01 00:00:00
abstract：:The current study presents Raman Spectroscopy (RS) accompanied by machine learning algorithms based on Principle Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) for analysis of tuberculosis (TB). TB positive (diseased), TB negative (cured) and control (healthy) serum samples are considered for inter a...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101963
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVES:The aim of this study was to develop a novel photosensitizer from traditional plant extracts and to investigate the photodynamic therapy (PDT) effect and mechanism of action of the novel photosensitizer on KB and Hep-2 cells. METHODS:Fluorescence emission, cell viability, and intracellular distribution of c...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2019.02.020
更新日期：2019-06-01 00:00:00
abstract：:Photodynamic therapy (PDT) was started in the People's Republic of China in the early 1980s after domestically produced hematoporphyrin derivative (HpD) became available. Since then, numerous photosensitizers have been synthesized and evaluated. Several promising ones have undergone clinical investigations and a few h...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/S1572-1000(06)00009-3
更新日期：2006-03-01 00:00:00
abstract：BACKGROUND:Photosensitizers in nanocarriers have been investigated for antimicrobial Photodynamic Therapy (aPDT). However, most studies are focused against microorganisms in planktonic or monospecies biofilm. Thus, this in vitro study evaluated the effect of aPDT using Chloroaluminium phthalocyanine (ClAlPc) in cationi...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.10.005
更新日期：2018-12-01 00:00:00
abstract：AIM:To assess the efficacy of phototherapy on bond strength and microleakage of bulk fill composites in comparison to conventional dentin surface treatments. MATERIALS AND METHODS:Ninety human third molars were divided into 3 groups (n = 30 each) according to surface conditioning treatment. Group 1 (Etch and Bond) EB,...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.12.014
更新日期：2019-03-01 00:00:00
abstract：AIM:The aim of the present clinical trial was to evaluate the efficacy of photodynamic therapy (PDT) as an adjunct to mechanical debridement (MD) in the treatment of severe peri-implantitis (PI) with abscess. MATERIALS AND METHODS:Selected individuals with severe PI were divided into two groups: Group A: received meth...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.101750
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND:Photodynamic therapy (PDT) has been determined to be a promising treatment modality in the most resistant tumors such as malignant melanoma. However, the key cytotoxic agent of PDT, -singlet oxygen (1O2) - represents a high risk of photodynamic-associated side effects e.g. skin photosensitization. Recently, ...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2018.05.017
更新日期：2018-09-01 00:00:00
abstract：:Circumscribed choroidal hemangioma (CCH) is rarely associated with choroidal neovascularization (CNV), especially in HIV-infected patients. And the therapy is still controversial. In this study, we discussed the mechanism, clinical feature and treatment of such lesions, based on multi-modality imaging evidences. Photo...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2014.04.005
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Bacterial resistance is a problem during the process of healing of infected wounds. As a therapy, photodynamic therapy (PDT) has broad-spectral antibacterial activity and non-selective action, which makes it possible to deal with antibiotic resistance.. Methylene blue is a commonly used medicine, but it is r...
journal_title：Photodiagnosis and photodynamic therapy
pub_type： 杂志文章
doi：10.1016/j.pdpdt.2020.102051
更新日期：2020-12-01 00:00:00
