IQGAP1 modulates the proliferation and migration of vascular smooth muscle cells in response to estrogen.


:Vascular smooth muscle cell (VSMC) proliferation and migration has been proven to be a critical event in the development of varicosity. Variations in estrogen levels, a pathological event related to age and pregnancy, play a role in the pathogenesis of varicosity. Previous studies have reported a different response of VSMCs following estrogen stimulation. However, the exact mechanisms involved have not yet been elucidated. In the present study, we examined the responses of lesion and normal VSMCs treated with 10(-8) M 17β-estradiol (E2) for 24 h. A differential effect of exposure to E2 was observed in these cells. IQ-domain GTPase-activating protein 1 (IQGAP1), a scaffold protein, was overexpressed in the lesion VSMCs and was shown to modulate VSMC proliferation and migration in response to E2. Furthermore, the increased expression of IQGAP1 was found to be intimately associated with a high activity of estrogen receptor α (ERα), which has been implicated in the regulation of VSMC physiological function. Additionally, we found that two critical kinases, Akt and extracellular signal-regulated kinase (ERK), mediated the activation of ERα and VSMC proliferation. According to our results, we thus concluded that high levels of IQGAP1 in VSMCs regulate the physiological reaction of the cells in response to estrogen exposure, and that kinases are involved in the process by mediating ERα activation. In view of the essential role of IQGAP1 in the physiological function of VSMCs, targeting this molecule may prove to be a promising strategy for the treatment of varicosity.


Int J Mol Med


Huang X,Jin Y,Zhou D,Xu G,Huang J,Shen L




Has Abstract


2015-05-01 00:00:00












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    abstract::We had shown previously that the novel, marine, anticancer compound dehydrothyrsiferol (DHT) does not modulate P-glycoprotein (P-gp) dependent drug efflux. Many chemotherapeutics with clinical impact are substrates for the structurally distant related membrane transport protein MRP1 (multidrug resistance-associated pr...

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    authors: Pec MK,Aguirre A,Fernández JJ,Souto ML,Dorta JF,Villar J

    更新日期:2002-11-01 00:00:00

  • Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line.

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    authors: Ruan J,Liu F,Chen X,Zhao P,Su N,Xie G,Chen J,Zheng D,Luo R

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  • Efavirenz induces autophagy and aberrant differentiation in normal human keratinocytes.

    abstract::Although efavirenz (EFV) is efficacious as an antiretroviral therapy when combined with other antiretroviral drugs, it may cause adverse clinical effects, including skin and mucosal eruptions, central nervous system complications, hepatotoxicity, renal failure and pulmonary complications. The present study investigate...

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    authors: Dong Q,Oh JE,Yi JK,Kim RH,Shin KH,Mitsuyasu R,Park NH,Kang MK

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    journal_title:International journal of molecular medicine

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    authors: González A,Martínez-Campa C,Alonso-González C,Cos S

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    authors: Jia HY,Shi Y,Luo LF,Jiang G,Zhou Q,Xu SZ,Lei TC

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    authors: Huang H,Wu Y,Fu W,Wang X,Zhou L,Xu X,Huang F,Wu Y

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    abstract::Thioredoxin domain‑containing 5 (TXNDC5) is reportedly overexpressed in colorectal cancer (CRC) and is therefore considered an oncogene. However, the role of TXNDC5 in CRC tumorigenesis remains unclear. The present study aimed to explore the role of TXNDC5 in CRC tumorigenesis in vitro and in vivo under hypoxic and no...

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    authors: Tan F,Zhu H,He X,Yu N,Zhang X,Xu H,Pei H

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    authors: Venditti A,Buccisano F,Del Poeta G,Battaglia A,Tamburini A,Bruno A,Adorno G,Caravita T,Santinelli S,Del Moro B,Epiceno AM,Ballatore G,Amadori S

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    authors: Han Y,Sun X,Kuang D,Tong P,Lu C,Wang W,Li N,Chen Y,Wang X,Dai J,Zhang H

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    authors: Ahmed KM,Shitara Y,Kuwano H,Takenoshita S,Uchimuro K,Shinozawa T

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    authors: Katsura A,Morishita A,Iwama H,Tani J,Sakamoto T,Tatsuta M,Toyota Y,Fujita K,Kato K,Maeda E,Nomura T,Miyoshi H,Yoneyama H,Himoto T,Fujiwara S,Kobara H,Mori H,Niki T,Ono M,Hirashima M,Masaki T

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  • Different patterns of nigrostriatal degeneration in tremor type versus the akinetic-rigid and mixed types of Parkinson's disease at the early stages: molecular imaging with 123I-FP-CIT SPECT.

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    journal_title:International journal of molecular medicine

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    authors: Toth B,Schwacha MG,Kuebler JF,Bland KI,Wang P,Chaudrya IH

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    authors: He Z,Du X,Wu Y,Hua L,Wan L,Yan N

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    authors: Nagatsuka N,Harada K,Ando M,Nagao K

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  • Suppressing serum response factor inhibits invasion in cervical cancer cell lines via regulating Egr‑1 and epithelial-mesenchymal transition.

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    authors: Abe M,Kajino K,Akbar SM,Yamamura K,Onji M,Hino O

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Chi L,Peng L,Pan N,Hu X,Zhang Y

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Martinez-Glez V,Bello MJ,Franco-Hernandez C,De Campos JM,Isla A,Vaquero J,Rey JA

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  • Baicalin attenuates inflammation in mice with OVA-induced asthma by inhibiting NF-κB and suppressing CCR7/CCL19/CCL21.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Liu J,Wei Y,Luo Q,Xu F,Zhao Z,Zhang H,Lu L,Sun J,Liu F,Du X,Li M,Wei K,Dong J

    更新日期:2016-11-01 00:00:00

  • miR-214 regulates papillary thyroid carcinoma cell proliferation and metastasis by targeting PSMD10.

    abstract::MicroRNAs (miRNAs) have important effects on cancer occurrence and development by adjusting gene expression. The aim of the present study was to examine the role of miR‑214 in papillary thyroid carcinoma cell proliferation and metastasis, and its molecular mechanisms. miR‑214 was demonstrated to be markedly downregula...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Liu F,Lou K,Zhao X,Zhang J,Chen W,Qian Y,Zhao Y,Zhu Y,Zhang Y

    更新日期:2018-12-01 00:00:00

  • MicroRNA-4649-3p inhibits cell proliferation by targeting protein tyrosine phosphatase SHP-1 in nasopharyngeal carcinoma cells.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Pan X,Peng G,Liu S,Sun Z,Zou Z,Wu G

    更新日期:2015-08-01 00:00:00

  • KRAB-containing zinc finger gene ZNF268 encodes multiple alternatively spliced isoforms that contain transcription regulatory domains.

    abstract::The ZNF268 gene was originally isolated from an early human embryo cDNA library. Several different transcripts have been isolated for the ZNF268 gene and developmental expression studies suggest that ZNF268 plays a role in the development of human fetal liver and the differentiation of blood cells. In our effort to st...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Shao H,Zhu C,Zhao Z,Guo M,Qiu H,Liu H,Wang D,Xue L,Gao L,Sun C,Li W

    更新日期:2006-09-01 00:00:00

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    abstract::Liver ischemia represents a common clinical problem. In the present study, using an in vitro model of hepatic ischemia-reperfusion injury, we evaluated the potential cytoprotective effect of the purine metabolites, such as adenosine and inosine, and studied the mode of their pharmacological actions. The human hepatoce...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Módis K,Gerő D,Stangl R,Rosero O,Szijártó A,Lotz G,Mohácsik P,Szoleczky P,Coletta C,Szabó C

    更新日期:2013-02-01 00:00:00

  • Characterization of the nuclear localization signal in the DNA helicase responsible for Bloom syndrome.

    abstract::Bloom syndrome (BS) is a rare genetic disorder characterized by small body size, photosensitivity, immunodeficiency and a high predisposition to various types of cancer. BLM was identified as the causative gene for BS. The BLM protein is homologous to DNA helicase and has two basic amino acid clusters in its C-termina...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Hayakawa S,Kaneko H,Fukao T,Kasahara K,Matsumoto T,Furuichi Y,Kondo N

    更新日期:2000-05-01 00:00:00