Abstract:
:Diabetes is characterized by altered metabolism of key molecules and regulatory pathways. The phenotypic expression of diabetes and associated complications encompasses complex interactions between genetic, environmental, and tissue-specific factors that require an integrated understanding of perturbations in the network of genes, proteins, and metabolites. Metabolomics attempts to systematically identify and quantitate small molecule metabolites from biological systems. The recent rapid development of a variety of analytical platforms based on mass spectrometry and nuclear magnetic resonance have enabled identification of complex metabolic phenotypes. Continued development of bioinformatics and analytical strategies has facilitated the discovery of causal links in understanding the pathophysiology of diabetes and its complications. Here, we summarize the metabolomics workflow, including analytical, statistical, and computational tools, highlight recent applications of metabolomics in diabetes research, and discuss the challenges in the field.
journal_name
Diabetesjournal_title
Diabetesauthors
Sas KM,Karnovsky A,Michailidis G,Pennathur Sdoi
10.2337/db14-0509subject
Has Abstractpub_date
2015-03-01 00:00:00pages
718-32issue
3eissn
0012-1797issn
1939-327Xpii
db14-0509journal_volume
64pub_type
杂志文章,评审相关文献
DIABETES文献大全abstract::Fourteen rats of the spontaneously diabetic BB line were bled from the retroorbital sinus approximately every 10 days. Sera taken from an early age up to 20 days after the onset of overt diabetes were assayed for complement-fixing antibodies against antigens of the surface of islet cells (CFA). Dispersed islet cells f...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.1.93
更新日期:1984-01-01 00:00:00
abstract::The plasma concentration of 1,5-anhydro-D-glucitol (AG) was measured in 135 newly diagnosed patients who were referred for oral glucose tolerance tests. AG concentrations in the nondiabetic patients indicated that the mean value of normal AG concentration was 21.8 micrograms/ml (SD = 5.9 micrograms/ml, range 9.6-38.8 ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.36.6.709
更新日期:1987-06-01 00:00:00
abstract::We conducted autosomal genome scans to map loci for metabolic syndrome (MES) and related traits in the Hong Kong Family Diabetes Study. We selected 55 families with 137 affected members (121 affected relative pairs) for nonparametric linkage analysis on MES. We also selected 179 families with 897 members (2,127 relati...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.10.2676
更新日期:2004-10-01 00:00:00
abstract::The selective impairment of glucose-induced insulin secretion in NIDDM can be attributed to defects in the glucose-signaling system. An alteration in the activity of phosphofructokinase (PFK), a key enzyme in the glycolytic pathway, may play a role in the abnormal glucose-induced insulin secretion. In this study, we e...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.46.9.1414
更新日期:1997-09-01 00:00:00
abstract::The uptake of 2-deoxy-D-[1-14C]-glucose, in the presence and absence of insulin, was measured in adipocytes from normal and streptozotocin-diabetic animals, over a wide temperature range (25-45 degrees C). Optimum temperatures for uptake in the presence of maximally stimulating insulin concentrations occurred near phy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.3.258
更新日期:1984-03-01 00:00:00
abstract::We investigated the effects of obesity surgery-induced weight loss on transcription factor 7-like 2 gene (TCF7L2) alternative splicing in adipose tissue and liver. Furthermore, we determined the association of TCF7L2 splicing with the levels of plasma glucose and serum free fatty acids (FFAs) in three independent stud...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-0239
更新日期:2012-11-01 00:00:00
abstract::Islets from male B10.BR mice (H-2k) were isolated by the collagenase technique, handpicked with a Pasteur pipette, and incubated (either intact or after dispersion with Dispase) for 0, 3, 5, 7, 10, or 14 days in tissue culture medium supplemented with either lymphokine supernatants or recombinant murine interferon-gam...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.35.10.1174
更新日期:1986-10-01 00:00:00
abstract:OBJECTIVE:Evidence links the hypothalamic fatty acid synthase (FAS) pathway to the regulation of food intake and body weight. This includes pharmacological inhibitors that potently reduce feeding and body weight. The mammalian target of rapamycin (mTOR) is an intracellular fuel sensor whose activity in the hypothalamus...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-1690
更新日期:2008-12-01 00:00:00
abstract::Two major metabolic perturbations, increased polyol (sorbitol) pathway activity and reduced tissue myo-inositol content, are induced in peripheral nerve by hyperglycemia. Although they are commonly invoked as alternative biochemical pathogenetic mechanisms for diabetic neuropathy, their possible interrelationship has ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.11.988
更新日期:1983-11-01 00:00:00
abstract::In pancreatic beta-cells, glucose metabolism signals insulin secretion by altering the cellular array of messenger molecules. ATP is particularly important, given its role in regulating cation channel activity, exocytosis, and events dependent upon its hydrolysis. Uncoupling protein (UCP)-2 is proposed to catalyze a m...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.6.1302
更新日期:2001-06-01 00:00:00
abstract::Insulin and glucagon release and insulin sensitivity were investigated in patients with glucokinase deficiency. Five subjects with a missense mutation (Glu256Lys) were studied. They were compared with six healthy subjects with low insulin response but normal glucose tolerance. Insulin and glucagon levels were measured...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.12.1402
更新日期:1994-12-01 00:00:00
abstract::Type 1 diabetes (T1D) remains a major health problem worldwide, with a steadily rising incidence yet no cure. Phosphoinositide 3-kinase-γ (PI3Kγ), a member of a family of lipid kinases expressed primarily in leukocytes, has been the subject of substantial research for its role in inflammatory diseases. However, the ro...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-0134
更新日期:2012-06-01 00:00:00
abstract::Immune modulation of pancreatic inflammation induces recovery from type 1 diabetes (T1D), but remission was not durable, perhaps because of an inability to sustain the formation and function of new pancreatic β-cells. We have previously shown that Ig-GAD2, carrying GAD 206-220 peptide, induced in hyperglycemic mice im...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-1281
更新日期:2013-08-01 00:00:00
abstract::We report a genomewide linkage analysis of a large consanguineous family segregating autosomal recessively inherited neonatal diabetes and the identification of a novel neonatal diabetes locus. Neonatal diabetes was characterized by low levels of circulating C-peptide with very low to undetectable levels of insulin in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.52.10.2636
更新日期:2003-10-01 00:00:00
abstract::Peptide-YY (PYY) is secreted from endocrine L-cells of the gastrointestinal tract in response to caloric ingestion and may mediate postprandial satiety through the hypothalamic neuropeptide Y2 receptor (Y2R). We examined whether variants in the genes encoding PYY and Y2R might be associated with obesity-related phenot...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.9.2461
更新日期:2004-09-01 00:00:00
abstract::Diabetic nephropathy (DN) is the major life-threatening complication of diabetes. Abnormal permselectivity of glomerular basement membrane (GBM) plays an important role in DN pathogenesis. Heparanase is the predominant enzyme that degrades heparan sulfate (HS), the main polysaccharide of the GBM. Loss of GBM HS in dia...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db11-1024
更新日期:2012-01-01 00:00:00
abstract::We have recently reported an A to G transition at nucleotide position 3243 in the mitochondrial DNA (mtDNA) tRNA(Leu(UUR)) gene in a large family with non-insulin-dependent diabetes mellitus (NIDDM). Characteristic was its maternal transmission and an associated sensorineural hearing loss. In a screening of a Dutch an...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.43.6.746
更新日期:1994-06-01 00:00:00
abstract::High glucose concentrations such as are seen in diabetes mellitus are known to have deleterious effects on cells, but the pathways by which glucose induces these effects are unknown. One hypothesis is that metabolism of glucose to glucosamine might be involved. For example, it has been shown that glucosamine is more p...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.42.9.1289
更新日期:1993-09-01 00:00:00
abstract::We conducted a prospective study to describe the course of the pancreatic beta-cell function from the time of clinical diagnosis of insulin-dependent (type I) diabetes to determine whether DR type, presence of islet cell antibodies (ICA), presence of insulin antibodies (IA), age at onset, and sex could help in the pre...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.7.920
更新日期:1988-07-01 00:00:00
abstract::Adiponutrin is one of three recently identified adipocyte lipases. Surprisingly, these proteins also retain transacylase activity, a hitherto unknown pathway of triacylglycerol synthesis in the adipocytes. This may enable them to participate in both anabolic and catabolic processes. The adiponutrin gene (ADPN) is down...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.55.03.06.db05-1075
更新日期:2006-03-01 00:00:00
abstract::It is unknown whether and to what extent changes in various endothelial functions and adrenergic responsiveness are related to the development of microvascular complications in type 1 diabetes. Therefore, endothelium-dependent and endothelium-independent vasodilatation, endothelium-dependent hemostatic factors, and on...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.6.1300
更新日期:1999-06-01 00:00:00
abstract::Recent work suggests that insulin may exert both positive and negative feedback directly on pancreatic beta-cells. To investigate the hypothesis that insulin modulates beta-cell metabolism, mouse islets and beta-cell clusters were loaded with rhodamine 123 to dynamically monitor mitochondrial membrane potential (Delta...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.53.7.1765
更新日期:2004-07-01 00:00:00
abstract::Unacylated ghrelin (UAG) is the predominant ghrelin isoform in the circulation. Despite its inability to activate the classical ghrelin receptor, preclinical studies suggest that UAG may promote β-cell function. We hypothesized that UAG would oppose the effects of acylated ghrelin (AG) on insulin secretion and glucose...
journal_title:Diabetes
pub_type: 杂志文章,随机对照试验
doi:10.2337/db13-1598
更新日期:2014-07-01 00:00:00
abstract::Glucokinase from rat liver or transplantable, radiation-induced insulinomas was partially purified by ion exchange chromatography using DEAE-Cibacron Blue F3GA agarose. Phosphorylation of alpha,beta-D-mannose by glucokinase occurred with cooperative rate dependence on mannose concentration (nH: 1.50). Half-maximal pho...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1146
更新日期:1983-12-01 00:00:00
abstract::Toll-like receptor 9 (TLR9) is highly expressed in B cells, and B cells are important in the pathogenesis of type 1 diabetes (T1D) development. However, the intrinsic effect of TLR9 in B cells on β-cell autoimmunity is not known. To fill this knowledge gap, we generated NOD mice with a B-cell-specific deficiency of TL...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0373
更新日期:2021-02-01 00:00:00
abstract::In renal transplant recipients (RTRs), new-onset diabetes after transplantation (NODAT) is a frequent and serious complication limiting survival of graft and patient. However, the underlying pathophysiology remains incompletely understood. In vitro and in preclinical models, HDL can preserve β-cell function, largely b...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-1267
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Skeletal muscle protein metabolism is resistant to the anabolic action of insulin in healthy, nondiabetic older adults. This defect is associated with impaired insulin-induced vasodilation and mTORC1 signaling. We hypothesized that, in older subjects, pharmacological restoration of insulin-induced capillary r...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-0415
更新日期:2010-11-01 00:00:00
abstract::Diabetogenic strains of Coxsackievirus B4 (CB4) produce a diabetes syndrome in susceptible mice that resembles insulin-dependent diabetes mellitus. To assess the possible role of autoimmunity, the expression of a 64,000-Mr islet antigen in SJL/J and CD1 mice infected with a diabetogenic strain of CB4 was monitored in ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.10.1419
更新日期:1988-10-01 00:00:00
abstract::Glucagon-like peptide 1 receptor (GLP-1R) imaging with radiolabeled exendin has proven to be a powerful tool to quantify β-cell mass (BCM) in vivo. As GLP-1R expression is thought to be influenced by glycemic control, we examined the effect of blood glucose (BG) levels on GLP-1R-mediated exendin uptake in both murine ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db20-0616
更新日期:2020-11-01 00:00:00
abstract::Different degrees of beta-cell failure and apoptosis are present in type 1 and type 2 diabetes. It has been recently suggested that high glucose-induced beta-cell apoptosis in type 2 diabetes shares a final common pathway with type 1 diabetes, involving interleukin-1beta (IL-1beta) production by beta-cells, nuclear fa...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.54.11.3238
更新日期:2005-11-01 00:00:00