Abstract:
:Chromatographic gel filtration matrixes used in various separation techniques are subject to microbial contamination. The need for a microbe-free column is critical when preparing materials that require a low or zero microbial count. This report proposes two alternative washing systems: 0.02 N HCl containing 0.81% NaCl, and 0.1 M tromethamine--hydrochloride buffer (pH 7.0) containing 0.81% NaCl and 0.02% thimerosal. These washing systems were validated using a 100 x 2.6-cm column packed with a modified dextran gel slurry previously inoculated with known counts of USP test organisms. After each wash, the column separation characteristics were verified further with appropriate test proteins.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Wen LF,Bohannon T,Crabb A,Mamaril Fdoi
10.1002/jps.2600690205subject
Has Abstractpub_date
1980-02-01 00:00:00pages
140-4issue
2eissn
0022-3549issn
1520-6017pii
S0022-3549(15)43076-Xjournal_volume
69pub_type
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