Abstract:
:T cell activation involves events at the plasma membrane; therefore, molecules such as long chain omega-3 fatty acids that alter the structure of the plasma membrane may affect the activation of aged T cells. In this project we investigated whether the incorporation of omega-3 fatty acids (from fish oil), in the presence of vitamin E, improves age-diminished T cell proliferation. Young and old mice were fed diets rich in either fish (menhaden) oil or saturated fat for various lengths of time. Splenocytes were harvested from these mice and stimulated in culture with either mitogen or the antigen keyhole limpet hemocyanin (for a secondary response); proliferation was estimated by [3H]thymidine incorporation. We found no discernible effect of dietary omega-3 fatty acids (with vitamin E supplementation) on lymphocyte proliferation stimulated by the mitogens concanavalin A or phytohemagglutinin. We did, however, find that the saturated fat diet and the menhaden oil diet in young mice lowered protein kinase C activities in the particulate fractions of spleen cells when compared to chow-fed mice. Middle-aged and old mice were less affected by the experimental diets than young mice, but they demonstrated decreased protein kinase C activity as well. These alterations did not affect the ability of splenocytes to respond to mitogenic stimulation. Fatty acid analysis revealed that lymphocytes from mice fed saturated fat for 8.5 months retained significant amounts of the omega-3 fatty acid docosahexaenoic acid, despite the lack of dietary omega-3 fatty acids. However, when aged (but not young) lymphocytes were clonally expanded by antigen in vivo in the presence of dietary omega-3 fatty acids, they produced a greater secondary proliferative response than old lymphocytes expanded during a saturated fat diet. Although our results suggest that omega-3 fatty acids may enhance aged lymphocyte proliferation, the tenacious retention of these fatty acids makes comparison with omega-3-depleted lymphocytes difficult.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
VanMeter AR,Ehringer WD,Stillwell W,Blumenthal EJ,Jenski LJdoi
10.1016/0047-6374(94)90079-5subject
Has Abstractpub_date
1994-08-01 00:00:00pages
95-114issue
2eissn
0047-6374issn
1872-6216pii
0047-6374(94)90079-5journal_volume
75pub_type
杂志文章abstract::Human cytomegalovirus (HCMV) establishes a highly prevalent life-long latent infection. Though generally subclinical, HCMV infection may have severe consequences during fetal development and in immunocompromised individuals. Based on epidemiological studies HCMV(+) serology has been associated with the development of ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/j.mad.2016.06.010
更新日期:2016-09-01 00:00:00
abstract::The mTOR deregulation has a role in chronic kidney disease including diabetic nephropathy. SIRT1 is an important participant in renal cytoprotective responses to aging and stress. However, whether both mTOR and SIRT1 are involved in high glucose-inducing mesangial cells (MCs) senescence still remains to be explored. H...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2012.04.005
更新日期:2012-06-01 00:00:00
abstract::There are multiple lines of evidence showing that oxidative stress and aberrant mitogenic signaling play an important role in the pathogenesis of Alzheimer disease. However, the chronological relationship between these and other events associated with disease pathogenesis is not known. Given the important role that mi...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00342-6
更新日期:2001-12-01 00:00:00
abstract::Several alternative mortality models fit Swedish old-age mortality data equally well. The models build on two different concepts of the heterogeneity of individuals in a population. The first concept concerns fixed, genetic differences among individuals in their risk of death. The second concept involves acquired susc...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)90094-9
更新日期:1994-05-01 00:00:00
abstract::Oxidative damage to macromolecules, including lipids, has been hypothesized as a mechanism of aging. One end product of lipid peroxidation, malondialdehyde (MDA), is often quantified as a measure of oxidative damage to lipids. We used a commercial colorimetric assay for MDA (Bioxytech LPO-586, Oxis International, Port...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00329-3
更新日期:2002-01-01 00:00:00
abstract::Analysis was made of the cellular basis of decline in the antibody immune response with advancing age. Employing the cell transfer system, it was found that spleen cells of aged mice have a limited capacity to react to SRBC when transferred into young irradiated recipients. Partial reconstitution was achieved when thy...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90073-8
更新日期:1978-04-01 00:00:00
abstract::The activity of RU 41740, a glycoprotein extract from Klebsiella pneumoniae has been investigated on some polymorphonuclear (PMN) functions. Chemotaxis, random migration and oxidative metabolism (assessed by chemiluminescence, O2 consumption and O2- generation) were studied in parallel. PMN were collected from adult a...
journal_title:Mechanisms of ageing and development
pub_type: 临床试验,杂志文章
doi:10.1016/0047-6374(88)90023-1
更新日期:1988-09-01 00:00:00
abstract::Age-adjusted mortality rates for breast cancer (BC) in the United States from 1962 to 1987 were subjected to longitudinal Gompertzian analysis. Age-adjusted BC mortality rate distributions for women display two distinct Gompertzian slopes. Between age 15 and 40 years, age-adjusted BC mortality rate distributions inter...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(91)90125-j
更新日期:1991-10-01 00:00:00
abstract::The ascidian Ciona intestinalis has a short life span and powerful regeneration capacities. The regeneration of the oral siphon (OS) involves wound healing, blastema formation, cell proliferation, and replacement of 8 oral pigment organs (OPO), the latter via differentiation and migration of stem/precursor cells from ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2012.08.003
更新日期:2012-09-01 00:00:00
abstract::The bone marrow content, proliferative potential and proliferative activity of precursor cells for stromal fibroblast colony forming cells (CFC-F) were investigated in young and old CBA mice. The relationship between CFC-F and the number of bone marrow nucleated cells and granulocytic-macrophagal precursors (GM-CFC) w...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90061-j
更新日期:1990-05-30 00:00:00
abstract::Mouse mammary gland displays an irreversible decline in growth rate when propagated by serial transplantation in gland-free mammary fat pads of isogeneic mice. Because transplanted fragments of gland contain both mammary epithelial and stromal elements, the present study was undertaken to distinguish between two possi...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(83)90026-x
更新日期:1983-11-01 00:00:00
abstract::The purpose of these studies was to analyze the role of different immune cell populations in the immune response against Trypanosoma cruzi antigens in aged mice. Mice of different ages (3 and 12 months old) were immunized i.d. with S-105 plus Bordetella pertussis as adjuvant and we compared the activities of the lymph...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(96)01719-8
更新日期:1996-07-05 00:00:00
abstract::A few reports suggest that pigmented granules found in molluscan neurons accumulate with age as do lipofuscin granules in vertebrate cells; however, no reports on molluscan neurons include detailed descriptions of granule accumulation or histochemical tests to identify the pigment as lipofuscin-like. In this study lig...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(78)90052-0
更新日期:1978-01-01 00:00:00
abstract::Protein carbonyls were studied in aging and exercise by immunoblot followed by one- or two-dimensional polyacrylamide gel electrophoresis using antibodies against 2,4-dinitrophenylhydrazones. Proteins of rat kidneys exhibited significant age-related increase in the amount of carbonyl while those of the brain and liver...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/s0047-6374(98)00133-x
更新日期:1999-03-15 00:00:00
abstract::The kinetics of accumulation and loss of zinc from the liver following subcutaneous administration of 10 mg of zinc per kg were examined in young adult (6 months old) and old (24 months old) male C57BL/6J mice. After zinc treatment, total liver zinc concentrations rose equally in both groups and returned to basal leve...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(89)90036-5
更新日期:1989-03-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(93)90043-q
更新日期:1993-10-01 00:00:00
abstract::Hutchinson-Gilford Progeria syndrome (HGPS) is a rare genetic disorder that displays features of segmental aging. It is manifested predominantly in connective tissue, with most prominent histological changes occurring in the skin, cartilage, bone and cardiovascular tissues. Detailed quantitative real time reverse-tran...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2006.03.004
更新日期:2006-08-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2006.01.004
更新日期:2006-05-01 00:00:00
abstract::Aging is a biological process associated with impairment of mitochondrial bioenergetic function, increased oxidative stress, attenuated ability to respond to stresses and increased risk in contracting age-associated diseases. When mitochondria are subjected to oxidative stress, accompanied by calcium overload and ATP ...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2012.12.006
更新日期:2013-01-01 00:00:00
abstract::Cultured epiphyseal-chondrocytes from embryonic chick may serve as a useful in vitro model to study aging processes in cartilage. The accelerated aging process in cultured chondrocytes is completed within a month and is manifested by typical changes in both cellular and extracellular compartments. Under common mainten...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(88)90105-4
更新日期:1988-11-14 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2019.111150
更新日期:2019-12-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2020.111364
更新日期:2020-12-01 00:00:00
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journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mad.2017.04.002
更新日期:2017-06-01 00:00:00
abstract::The increased lifespan caused by food limitation has been observed in a wide range of animals including the nematode Caenorhabditis elegans. We show here that the lifespans of eat-2 and eat-5 feeding-defective mutants and a mutant of dbl-1 encoding a TGFβ ligand significantly change between the cultures fed on Escheri...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2011.02.005
更新日期:2011-04-01 00:00:00
abstract::As the endothelium ages it may become more susceptible to damage by atherogenic plasma components such as toxic lipid oxidation products. Vitamin E (vit E) might prove to be anti-atherogenic by reducing oxidative injury. This study investigated the effects of age and chronic exposure to fatty acid hydroperoxides (OFA)...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(90)90110-2
更新日期:1990-10-01 00:00:00
abstract::There is an alteration of the immune response in aging that leads to the increased incidence of infections, cancers and autoimmune disorders. The aim of the present study was to investigate whether there exists changes in signal transduction under the IL-2 receptor stimulation and the role of plasma membrane cholester...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(01)00274-3
更新日期:2001-09-15 00:00:00
abstract::Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-m...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/0047-6374(94)01553-x
更新日期:1995-04-21 00:00:00
abstract::Age related change of the human minor pectoral muscles was biochemically demonstrated. Myosin-ATPase activity was significantly decreased with age, and was activated with Tris. The degree of the activation by Tris was observed to be lower in old aged patients than in the young. Furthermore, at low concentration of CaC...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/s0047-6374(85)80029-4
更新日期:1985-07-01 00:00:00
abstract::The effects of long-term caloric restriction on the hepatic phase II drug metabolizing enzymes were investigated in the male Fischer 344 rat. Rats that had been restricted to 60% of their pair-fed control consumption from 14 weeks post-partum exhibited altered conjugating enzyme activities at 22 months. Caloric restri...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章,评审
doi:10.1016/0047-6374(89)90047-x
更新日期:1989-05-01 00:00:00
abstract::There are reports demonstrating elevated levels of autoantibodies in elderly people. We now analyzed whether the strong inflammatory response associated with aging is interrelated with the production of autoantibodies, antinuclear antibodies (ANA). In a cohort of 284 nonagenarians the rate of ANA positivity was 12.3%,...
journal_title:Mechanisms of ageing and development
pub_type: 杂志文章
doi:10.1016/j.mad.2007.03.001
更新日期:2007-05-01 00:00:00