Performance of a new DNA probe for the detection of group B streptococcal colonization of the genital tract.

Abstract:

OBJECTIVE:To test the performance characteristics of a new DNA probe designed for the rapid identification of heavy colonization of the genital tract with group B streptococci. METHODS:Vaginal and combined vaginal-perianal samples were collected from 193 pregnant women and cultured on colistin-nalidixic acid agar plates. Bacterial growth was classified semiquantitatively. Specimens were also tested by a new DNA probe in two formats: a direct assay performed on the swabs soon after collection and an assay performed after the swabs were incubated for 24 hours in an enriched culture medium. RESULTS:The agar cultures were positive in 36 of 193 patients (18.6%, 95% confidence interval 13.2-24). Nineteen women were lightly colonized, and 17 were heavily colonized (at least 10(4) colonies/mL). The combined vaginal-perianal swabs yielded positive results more often than the vaginal swabs alone (26 versus 16, chi 2 = 24, P < .01). In its direct form, the assay had only 8.3% sensitivity in identifying colonized women. In heavily colonized women, the sensitivity of the assay increased slightly to 12%. After a 16-24-hour incubation, the sensitivity of the assay was 81%. CONCLUSION:The direct assay is insufficiently sensitive for clinical use. The delayed assay offers no advantage over standard cultures.

journal_name

Obstet Gynecol

authors

Rosa C,Clark P,Duff P

doi

10.1016/0029-7844(95)00199-2

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

509-11

issue

4 Pt 1

eissn

0029-7844

issn

1873-233X

journal_volume

86

pub_type

临床试验,杂志文章
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    authors: Bachmann GA,Trattler B,Ko T,Tweddel G

    更新日期:1998-07-01 00:00:00

  • Postpartum Blood Loss in Women Treated for Intrahepatic Cholestasis of Pregnancy.

    abstract:OBJECTIVE:To evaluate postpartum blood loss in women with treated intrahepatic cholestasis of pregnancy. METHODS:In a retrospective case-control study, 15,083 deliveries including 348 women with intrahepatic cholestasis of pregnancy (2.3%) were analyzed from 2004 to 2014. To adjust for differences in baseline characte...

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    doi:10.1097/AOG.0000000000001693

    authors: Furrer R,Winter K,Schäffer L,Zimmermann R,Burkhardt T,Haslinger C

    更新日期:2016-11-01 00:00:00

  • Abortion at 18-22 weeks by laminaria dilation and evacuation.

    abstract:OBJECTIVE:To evaluate the complications of late second-trimester abortions (18-22 weeks) by laminaria dilation and evacuation, and the obstetric outcome of subsequent pregnancies. METHODS:Dilation of the cervix was achieved by repeated laminaria tent replacement. Evacuation was carried out in the outpatient clinic usi...

    journal_title:Obstetrics and gynecology

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    doi:10.1016/0029-7844(96)00170-6

    authors: Schneider D,Halperin R,Langer R,Caspi E,Bukovsky I

    更新日期:1996-09-01 00:00:00

  • Perinatal infection with Torulopsis glabrata: a case associated with maternal sickle cell anemia.

    abstract::Torulopsis glabrata is a yeast of low virulence and commensal within the female genitourinary tract. The first case of congenital infection with Torulopsis glabrata was reported in 1980. An additional example of perinatal Torulopsis infection which is associated with maternal sickle cell anemia is reported. The most c...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Sander CH,Martin JN,Rogers AL,Barr M Jr,Heidelberger KP

    更新日期:1983-03-01 00:00:00

  • Fetal nucleated cells in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage.

    abstract:OBJECTIVE:To determine how long fetal nucleated cells can be detected in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage, using fluorescence in situ hybridization or polymerase chain reaction. METHODS:Nucleated cells with Y chromosomes were analyzed in 90 blood samples using fluorescence i...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/0029-7844(94)00414-9

    authors: Hamada H,Arinami T,Hamaguchi H,Kubo T

    更新日期:1995-03-01 00:00:00

  • Midtrimester abortion by intraamniotic prostaglandin F2alpha. Safer than saline?

    abstract::Published reports disagree on the risks of intraamniotic prostaglandin F2alpha (PGF2alpha) for midtrimester abortion. To evaluate the safety and efficacy of intraamniotic PGF2alpha, we compared 1241 PGF2alpha and 10,013 saline abortions performed during the 13th to the 24th menstrual week. The results were reported in...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Grimes DA,Schulz KF,Cates W Jr,Tyler CW Jr

    更新日期:1977-05-01 00:00:00

  • Customized Pessary Fabrication Using Three-Dimensional Printing Technology.

    abstract:BACKGROUND:Pessaries are a treatment option for pelvic organ prolapse, stress urinary incontinence (SUI), and cervical incompetence. An effective pessary is comfortable, corrects the presenting problem, does not cause adverse effects, and is easy to remove. Discomfort and poor fit limit the usefulness of pessaries for ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1097/AOG.0000000000002461

    authors: Barsky M,Kelley R,Bhora FY,Hardart A

    更新日期:2018-03-01 00:00:00

  • Detection of Chlamydia trachomatis infection by direct immunofluorescence staining of genital secretion.

    abstract::A monoclonal antibody specific for Chlamydia trachomatis (Syva Microtrak) was used to stain directly 249, 127, and 67 specimens obtained from the cervix, and the female and the male urethra, respectively. The Microtrak system identified 100% or 23 culture-positive cases from the cervix (prevalence 9.2), 100% or seven ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Wiesmeier E,Bruckner D,Black M

    更新日期:1987-03-01 00:00:00

  • Morbidity and mortality associated with primary and repeat operations for ovarian cancer.

    abstract::Aggressive surgery may improve the outcome of patients with ovarian cancer. To assess the risk of operative complications, we analyzed 472 primary and 299 repeat operations for ovarian cancer in 536 women between 14-91 years of age. Intraoperative bleeding estimated to be over 1000 mL (N = 107) was more common after p...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Venesmaa P,Ylikorkala O

    更新日期:1992-02-01 00:00:00

  • Intracorporeal electromechanical tissue morcellation: a critical review and recommendations for clinical practice.

    abstract::Electromechanical morcellators have come under scrutiny with concerns about complications involving iatrogenic dissemination of both benign and malignant tissues. Although the rapidly rotating blade has resulted in morcellator-related vascular and visceral injuries, equally concerning are the multiple reports in the l...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章,评审

    doi:10.1097/AOG.0000000000000448

    authors: Kho KA,Anderson TL,Nezhat CH

    更新日期:2014-10-01 00:00:00

  • Adenocarcinoma of the vagina in a patient with gonadal dysgenesis.

    abstract::Adenocarcinoma of the vagina is an uncommon tumor. Many theories have been postulated for its etiology and tissue origin. Its occurrence in young women exposed in utero to diethylstilbestrol has pointed to estrogen influence as a possible etiologic factor. A case of adenocarcinoma of the vagina in a patient with gonad...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Shingleton HM,Younger JB,Beasley WE,Levy D,Gore H,Lawrence WD

    更新日期:1979-03-01 00:00:00

  • Vaginal Cuff Dehiscence and Evisceration: A Review.

    abstract::Vaginal cuff dehiscence is an infrequent complication of hysterectomy, with the potential for evisceration and additional morbidity. This review aims to describe the incidence, risk factors, preventative measures, and management. Identification of specific risk factors is problematic because many studies either lack c...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章,评审

    doi:10.1097/AOG.0000000000002852

    authors: Nezhat C,Kennedy Burns M,Wood M,Nezhat C,Nezhat A,Nezhat F

    更新日期:2018-10-01 00:00:00

  • Mucinous cystadenocarcinoma of the retroperitoneum.

    abstract::A case of a primary retroperitoneal mucinous cystadenocarcinoma of ovarian type found at laparotomy in the presence of two normal ovaries is described. Careful examination of the tumor showed no evidence of ovarian tissue in the wall. The appearance of this tumor could be traced from areas of benign mucinous cystadeno...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Roth LM,Ehrlich CE

    更新日期:1977-04-01 00:00:00

  • Fetal stroke associated with elevated maternal anticardiolipin antibodies.

    abstract::Middle cerebral artery infarction explains some cases of congenital hemiparesis with or without neonatal stroke. The etiology of the stroke is often obscure. We describe two infants with imaging evidence of middle cerebral artery infarction whose mothers had elevated anticardiolipin antibody levels after delivery. We ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Silver RK,MacGregor SN,Pasternak JF,Neely SE

    更新日期:1992-09-01 00:00:00

  • Reliability of intrauterine pressure measurements.

    abstract::The reliability of intrauterine pressure measurements was studied by placing two catheters in the same uterus in women in labor. Nineteen parturients were studied, five with two Intran I catheters in different pockets of amniotic fluid, five with two Intran II catheters in different pockets, and nine with two Intran I...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Arulkumaran S,Yang M,Tien CY,Ratnam SS

    更新日期:1991-11-01 00:00:00

  • Detection of benign endometrial masses by endometrial stripe measurement in premenopausal women.

    abstract:OBJECTIVE:Our objective was to estimate whether a transvaginal endometrial stripe of less than 5 mm in premenopausal women would exclude endometrial polyps and intracavitary leiomyomata, thereby eliminating the need for sonohysterography. METHODS:Data from 206 consecutive sonohysterograms performed in premenopausal wo...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1097/01.AOG.0000130065.49187.c8

    authors: Breitkopf DM,Frederickson RA,Snyder RR

    更新日期:2004-07-01 00:00:00

  • Relaxin in human pregnancy serum measured with an homologous radioimmunoassay.

    abstract::This study reports serum levels of relaxin in normal and special-interest pregnancies using an homologous radioimmunoassay for human relaxin. The mean levels in uncomplicated antenatal patients were lower than those reported in studies using heterologous assays, but the trend in serum levels was similar. Serum levels ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Bell RJ,Eddie LW,Lester AR,Wood EC,Johnston PD,Niall HD

    更新日期:1987-04-01 00:00:00

  • Absorption kinetics of misoprostol with oral or vaginal administration.

    abstract:OBJECTIVE:To compare the pharmacokinetics of vaginal and oral administration of the prostaglandin E1 analogue, misoprostol. METHODS:Twenty women received 400-micrograms doses of misoprostol either orally or as tablets placed in the vagina. Serum levels of principal metabolite, misoprostol acid, were measured at 7.5, 1...

    journal_title:Obstetrics and gynecology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/S0029-7844(97)00111-7

    authors: Zieman M,Fong SK,Benowitz NL,Banskter D,Darney PD

    更新日期:1997-07-01 00:00:00

  • A prospective, controlled study of the effects of hormonal contraception on bone mineral density.

    abstract:OBJECTIVE:To compare the effect of depot medroxyprogesterone acetate (DMPA) and two types of oral contraceptives (OC) on bone mineral density (BMD) among women 18-33 years of age with those not using hormonal contraception. METHODS:Data from 155 women were analyzed. Depot medroxyprogesterone acetate was administered t...

    journal_title:Obstetrics and gynecology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/s0029-7844(01)01495-8

    authors: Berenson AB,Radecki CM,Grady JJ,Rickert VI,Thomas A

    更新日期:2001-10-01 00:00:00

  • Intent-to-treat analysis of stage Ib and IIa cervical cancer in the United States: radiotherapy or surgery 1988-1995.

    abstract:OBJECTIVE:To estimate the patterns of care and outcome of women with early cervical cancer in the United States based on surgical or radiation intent-to-treat principles. METHODS:The Surveillance, Epidemiology, and End Results 1995 public-use file was the data source. Subjects between the ages of 15 and 80 years at di...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1016/s0029-7844(00)01117-0

    authors: Brewster WR,Monk BJ,Ziogas A,Anton-Culver H,Yamada SD,Berman ML

    更新日期:2001-02-01 00:00:00

  • Prevention of postoperative adhesions in the rat by in situ photopolymerization of bioresorbable hydrogel barriers.

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    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Hill-West JL,Chowdhury SM,Sawhney AS,Pathak CP,Dunn RC,Hubbell JA

    更新日期:1994-01-01 00:00:00

  • Predicting painful or difficult intrauterine device insertion in nulligravid women.

    abstract:OBJECTIVE:To assess the relationship of preinsertion vaginal ultrasound assessment and menstrual and gynecologic history as predictors of difficult or painful intrauterine device insertion in nulligravid women. METHODS:Nulligravid women seeking contraception were invited to participate in this nonrandomized study and ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1097/AOG.0000000000000362

    authors: Kaislasuo J,Heikinheimo O,Lähteenmäki P,Suhonen S

    更新日期:2014-08-01 00:00:00

  • Peripartum changes in maternal cerebral blood flow velocity in normotensive and preeclamptic patients.

    abstract:OBJECTIVE:To compare the effect of delivery on cerebral blood flow velocity between normotensive and preeclamptic women, adjusting for mode of delivery and change in hematocrit values. METHODS:Twenty-three normotensive and 46 preeclamptic women had maternal middle cerebral blood flow velocity assessed antepartum and a...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Williams KP,McLean C

    更新日期:1993-09-01 00:00:00

  • Bias in the Peer Review Process: Can We Do Better?

    abstract::Peer review is the major method used by the scientific community to evaluate manuscripts and decide what is suitable for publication. However, this process in its current design is not bulletproof and is prone to reviewer and editorial bias. Its lack of objectivity and transparency raise concerns that manuscripts migh...

    journal_title:Obstetrics and gynecology

    pub_type: 社论

    doi:10.1097/AOG.0000000000003260

    authors: Tvina A,Spellecy R,Palatnik A

    更新日期:2019-06-01 00:00:00

  • Anterior sacral meningocele in pregnancy.

    abstract::Anterior sacral meningocele is a rare abnormality. Only 14 cases of it have been reported in association with pregnancy. Of those 14 patients, three died as a result of rupture of the meningocele during labor and postpartum. A new case is presented; the clinical presentation, the diagnosis, and treatment are discussed...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Kofinas AD,Hatjis CG,Ernest JM,Parker RL Jr

    更新日期:1987-03-01 00:00:00

  • Health care provider attitudes and practices related to intrauterine devices for nulliparous women.

    abstract:OBJECTIVE:To examine predictors of health care providers perceiving intrauterine devices (IUDs) as unsafe for nulliparous women and of infrequent provision of IUDs to nulliparous women. METHODS:We analyzed questionnaire data obtained during December 2009 to March 2010 from 635 office-based providers (physicians) and 1...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1097/AOG.0b013e31824aca39

    authors: Tyler CP,Whiteman MK,Zapata LB,Curtis KM,Hillis SD,Marchbanks PA

    更新日期:2012-04-01 00:00:00

  • Rethinking Prenatal Screening for Anomalies of Placental and Umbilical Cord Implantation.

    abstract::The most common anomalies of implantation of the placenta and umbilical cord include placenta previa, placenta accreta spectrum, and vasa previa, and are associated with considerable perinatal and maternal morbidity and mortality. There is moderate quality evidence that prenatal diagnosis of these conditions improves ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:10.1097/AOG.0000000000004175

    authors: Jauniaux E,Silver RM

    更新日期:2020-12-01 00:00:00

  • The fetal sound environment during vibroacoustic stimulation in labor: effect on fetal heart rate response.

    abstract::Nine pregnant women near term, with medical indications for internal monitoring during labor, were studied to determine the transmission of sound across maternal soft tissues during vibroacoustic stimulation with an electronic artificial larynx. A miniaturized hydrophone was placed transcervically at the level of the ...

    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章

    doi:

    authors: Gagnon R,Benzaquen S,Hunse C

    更新日期:1992-06-01 00:00:00

  • External cephalic version for breech presentation with or without spinal analgesia in nulliparous women at term: a randomized controlled trial.

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    journal_title:Obstetrics and gynecology

    pub_type: 杂志文章,随机对照试验

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  • Follicular phase treatment of luteal phase defect with follicle-stimulating hormone in infertile women.

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    journal_title:Obstetrics and gynecology

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    doi:

    authors: Huang KE,Muechler EK,Bonfiglio TA

    更新日期:1984-07-01 00:00:00