Abstract:
OBJECTIVE:To determine how long fetal nucleated cells can be detected in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage, using fluorescence in situ hybridization or polymerase chain reaction. METHODS:Nucleated cells with Y chromosomes were analyzed in 90 blood samples using fluorescence in situ hybridization with a Y-chromosome-specific DNA probe. The samples were obtained from 15 mothers (gravida 1), who were delivered of normal male neonates at University of Tsukuba Hospital, Japan, with fetomaternal hemorrhage 1 and 7 days, and 1, 3, 6, and 12 months after delivery. The 15 mothers were selected if fetal cells were detected above the ratio of one fetal cell per 3000 mothers' cells at 1 day after delivery. Polymerase chain reaction amplifying two different Y-specific fragments (a 102 base-pair fragment in the pHY10 vector, which was a highly repetitive sequence, and a single-copy, Y-specific 198 base-pair fragment) was also carried out for the analysis of genomic DNA from the same samples. RESULTS:Seven days and 1 month after delivery, Y-positive cells were detected in nine and four of 15 samples, respectively. No cells with the hybridization signal were detected in any of the 45 samples obtained 3 months or more after delivery. When we performed the polymerase chain reaction experiment amplifying the single-copy Y-specific fragment, none of the 45 samples after 3 months or more showed the expected band. However, from the same 45 samples, four obtained from different mothers showed positive results by amplification of the fragment in the pHY10 vector. Because multiple samples taken from these mothers before the collection of the positive samples showed negative results in the three experiments, these findings strongly suggest that the observed positive results were false-positives. CONCLUSION:Fetal nucleated cells in maternal peripheral blood become undetectable 3 months after delivery in most, if not all, cases of fetomaternal hemorrhage.
journal_name
Obstet Gynecoljournal_title
Obstetrics and gynecologyauthors
Hamada H,Arinami T,Hamaguchi H,Kubo Tdoi
10.1016/0029-7844(94)00414-9subject
Has Abstractpub_date
1995-03-01 00:00:00pages
449-51issue
3eissn
0029-7844issn
1873-233Xpii
0029-7844(94)00414-9journal_volume
85pub_type
杂志文章abstract::A patient with non-mosaic Turner syndrome and normal fertility had four documented pregnancies, two of which were carried to term. Although her fertility history can be considered normal, she did not have spontaneous sexual maturation or menarche, and bilateral streak gonads were identified on laparotomy for tubal lig...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::Mississippi enacted medical negligence and other tort reform legislation that generally became effective for causes of action filed on or after January 1, 2003, and September 1, 2004. Data regarding lawsuits against physicians insured by the Medical Assurance Company of Mississippi (MACM), the largest medical liabilit...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0b013e318226ba47
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of medical legal risk on practice location of obstetrician-gynecologists. METHODS:We used the American College of Obstetricians and Gynecologists (ACOG) Membership Record to determine the number of Fellows and Junior Fellows by state. We obtained state malpractice premiums from the Me...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/01.AOG.0000157468.12907.1a
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:Abdominal pregnancies are rare types of ectopic pregnancies with high rates of maternal mortality and morbidity when encountered anywhere in the world. Combining such high-risk pregnancy with the limitations found in a low-resource setting can lead to increased complications, which can be more difficult to m...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000000706
更新日期:2015-05-01 00:00:00
abstract::The safety and efficacy of laminaria tents as an adjunct to labor induction near term were examined in a prospective, controlled, and randomized study involving insertion of laminaria into the cervical canal the evening prior to planned induction. Six of 35 subjects in whom laminaria were used entered labor spontaneou...
journal_title:Obstetrics and gynecology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00006250-197805000-00021
更新日期:1978-05-01 00:00:00
abstract:BACKGROUND:Fetomaternal hemorrhage is a rare cause of fetal anemia and hydrops fetalis. Early and severe fetomaternal hemorrhage may benefit from in utero transfusion(s); however, hemorrhage rate is unpredictable, and reliable criteria are needed to identify recurrent anemia. CASE:Fetal hydrops due to massive fetomate...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0b013e3181d99a93
更新日期:2010-05-01 00:00:00
abstract::Reported is a rare complication of radical hysterectomy and radiation therapy, recurrent streptococcal cellulitis. Two patients had 14 episodes over a nine-year period, characterized by high fever and systemic toxicity. The streptococcal cellulitis was atypical in its presentation and its causative organism. The rash ...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract:OBJECTIVE:To assess changes in the prescribing practices, knowledge, attitudes, and perceptions of health care providers after an educational program about emergency contraception. METHODS:Health care providers completed self-administered questionnaires before and 1 year after full implementation of the project. The 1...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0029-7844(01)01365-5
更新日期:2001-06-01 00:00:00
abstract:OBJECTIVE:To compare lamellar body counts with the lecithin/sphingomyelin ratio and phosphatidylglycerol analysis in terms of assessment of risk of respiratory distress syndrome (RDS). METHODS:Lamellar body counts, lecithin-sphingomyelin ratios (L/Ss), and phosphatidylglycerol levels were assessed in 1611 amniotic flu...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章,多中心研究
doi:10.1016/s0029-7844(00)01133-9
更新日期:2001-02-01 00:00:00
abstract:OBJECTIVE:To determine whether stage of disease and treatment patterns account for mortality differences between black and white women with cervical cancer. METHODS:Using data obtained from the Surveillance, Epidemiology, and End Results (SEER) Program for 1988-1994, we determined the associations between race and sta...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0029-7844(99)00334-8
更新日期:1999-10-01 00:00:00
abstract::Seventy-seven patients, presenting with radiation small bowel injuries at the University of Texas M. D. Anderson Hospital and Tumor Institute at Houston between 1962 and 1978, were analyzed retrospectively. The patients were divided into two categories: bowel bypass without resection, and resection of irradiated bowel...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract:OBJECTIVE:To identify characteristics associated with high inpatient daily opioid consumption after cesarean delivery. METHODS:This is a retrospective cohort study of all cesarean deliveries performed under neuraxial anesthesia with neuraxial morphine, at a single institution from January 1, 2015, to December 31, 2015...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000003087
更新日期:2019-02-01 00:00:00
abstract::Variability of the baseline fetal heart rate is correlated with good fetal outcome, and loss of baseline variability has been observed as a sign of fetal distress. Central nervous system depressing drugs may also decrease fetal heart rate variability, and thus recognition of the effect of medication on the baseline fe...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Twenty-seven patients with the diagnosis of primary carcinoma of the vagina were treated by definitive radiotherapy. Twenty-three patients received a combination of external and interstitial iridium-192 implant irradiation and four patients received only interstitial irradiation. Twenty-one patients had squamous cell ...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/00006250-198309000-00021
更新日期:1983-09-01 00:00:00
abstract::This month we focus on current research in validation and simulation training. Dr. Buek discusses four recent publications, which are concluded with a "bottom line" that is the take-home message. A complete reference for each can be found on on this page along with direct links to the abstracts. ...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000003592
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:To determine clinical characteristics of women with endometriosis-associated intraperitoneal cancers, to assess differences based on the relationship of the cancer to the endometriosis, and to assess factors associated with survival. METHODS:A search of medical records at The University of Texas M. D. Anders...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0029-7844(02)02149-x
更新日期:2002-10-01 00:00:00
abstract:OBJECTIVE:To develop and validate a predictive risk calculator for cesarean delivery among women undergoing induction of labor. METHODS:We performed a population-based cohort study of all women who had singleton live births after undergoing induction of labor from 32 0/7 to 42 6/7 weeks of gestation in the United Stat...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000003696
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Pulmonary sequestration is not believed to be familial. We report two male infants with this anomaly who were born to the same parents. CASES:The prenatal diagnosis of pulmonary sequestration was made in a woman's two consecutive pregnancies by demonstrating systemic arterial supply to an echogenic mass loc...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::The systolic/end-diastolic ratio (S/D) and pulsatility and resistance indices were calculated before and after diagnostic cordocentesis in 46 fetuses, after 19 intravascular transfusions in eight fetuses, and on two occasions 45 minutes apart in eight controls. The fetus was paralyzed with pancuronium (0.3 mg/kg sonog...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Rapid and accurate detection of neonatal bacteremia is an important part of the management of the neonate with suspected sepsis. This study compared the incidence of positive umbilical cord blood cultures (UCBCs) to the incidence of positive peripheral venous blood cultures and determined whether a meticulous UCBC tec...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract:OBJECTIVE:To systematically review studies examining the relationship between hyperemesis gravidarum and Helicobacter pylori (H pylori) infection. DATA SOURCES:A 1966 to January 2007 search using MEDLINE/PubMed, EMBASE, and Web of Science included MeSH terms: "Helicobacter pylori," "Helicobacter infections," "hypereme...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章,评审
doi:10.1097/01.AOG.0000278571.93861.26
更新日期:2007-09-01 00:00:00
abstract::Using the criteria proposed in 1982 to justify consideration of in utero surgical treatment of fetal disorders, the development of maternal-fetal treatment is described, and an assessment of current status is provided. Significant issues remain, and many questions are unanswered. By using three different disorders, co...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章,评审
doi:10.1097/AOG.0b013e318197c28f
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of cerebral velocimetry as a predictor of perinatal outcome in high-risk pregnancies. METHODS:Middle cerebral artery pulsatility index was measured in 576 high-risk pregnancies undergoing umbilical velocimetry. The results of both tests were evaluated with respect to the birth of small f...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0029-7844(97)00482-1
更新日期:1997-12-01 00:00:00
abstract:OBJECTIVE:To compare the consequences of sexual assault based on the relationship of the woman to her named assailant. METHODS:From January 2008 to March 2011, we conducted an observational and prospective study of females older than age 15 years who were examined at a sexual assault referral center. Data were collect...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000001288
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVE:To estimate the population risks of maternal and infant complications with the birth of macrosomic (at least 4000 g) compared with normal weight infants. METHODS:Term, singleton infants were identified from the state of Washington's birth event records database for 1990. Diagnosis codes from the Internal Cla...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1016/s0029-7844(98)00224-5
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Burkitt lymphoma during pregnancy is a rare event, and outcomes have been poor. However, few patients were treated by current standards, with nearly half receiving single-agent cyclophosphamide or no chemotherapy. CASE:A patient with Burkitt lymphoma presenting at 11 6/7 weeks' gestation was treated with su...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章,评审
doi:10.1016/s0029-7844(98)00282-8
更新日期:1998-10-01 00:00:00
abstract::This report describes an innovative use of videography in teaching retropubic space anatomy and surgery. After the standard abdominal incision has been made, the second surgical assistant places a gynecologic laparoscope, fitted with an endoscopic camera, into the retropubic space and guides the laparoscope documentin...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::This population-based case-control study assessed the effect of current use of monophasic or triphasic oral contraceptives (OCs) on the risk of functional ovarian cyst development. The cases were all 15-39-year-old enrollees in the Group Health Cooperative of Puget Sound who had either an inpatient primary diagnosis o...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Five hundred twelve patients who presented with initial atypical cervical cytology between February 1982 and February 1983 at Naval Hospital, San Diego were randomized with respect to antibiotic treatment and interval before repeat cytologic examination. Treatment before repeat cytology did not improve the rate of rev...
journal_title:Obstetrics and gynecology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1986-08-01 00:00:00
abstract::To prevent transmission of bloodborne pathogens, it is important that health care providers adhere to standard precautions, follow fundamental infection-control principles, and use appropriate procedural techniques. All obstetrician-gynecologists who provide clinical care should receive the hepatitis B virus vaccine s...
journal_title:Obstetrics and gynecology
pub_type: 杂志文章
doi:10.1097/AOG.0000000000001315
更新日期:2016-02-01 00:00:00