Abstract：
:The risk of pulmonary hemorrhage is increased in extremely low birth weight infants treated with surfactant. The pathogenesis of this increased risk is far from clear. We tested whether exposure of cell membranes to surfactant may lead to increased membrane permeability, hypothesizing that this process may contribute to the occurrence of alveolar hemorrhage after surfactant treatment. Aliquots of washed packed red blood cells (used as membrane model) were suspended in 0.9% NaCl with various concentrations of Survanta or Exosurf for either 2 or 24 h at 37 degrees C. Cytolysis was measured by spectrophotometric determination of free Hb after centrifugation. Red cells suspended in 0.9% NaCl alone, distilled water, or various concentrations of melittin were used as negative and positive controls. Both surfactants were associated with increased hemolysis to 35% of maximum at concentrations of 1.25 mg/2 mL. Above these concentrations, Survanta was associated with no increase in hemolysis, whereas Exosurf increased hemolysis to 60% of maximum at concentrations of 12.5 mg/2 mL. In additional experiments, primary cultures of alveolar type II cells from adult rats were treated with Survanta, Exosurf, the Exosurf components tyloxapol and hexadecanol, melittin, or culture medium alone. After 24 h of incubation, lactate dehydrogenase release into the media was measured as a percent of total lactate dehydrogenase activity to indicate cytotoxicity. Lactate dehydrogenase release was < 10% for control experiments but increased sharply with Exosurf and its components tyloxapol and hexadecanol. These results indicate that surfactant may be associated with in vitro cytotoxicity and that this property differs for different surfactants and different dosages.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Findlay RD,Taeusch HW,David-Cu R,Walther FJsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
26-30issue
1eissn
0031-3998issn
1530-0447journal_volume
37pub_type
杂志文章abstract：BACKGROUND:After birth, breastfeeding is the exclusive source of hormonal signaling between mother and infant. Hospitalized infants often receive donor milk when their own mother's milk is unavailable. METHODS:The presence of insulin, leptin, cortisol, progesterone, and testosterone was examined in samples from milk b...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1038/s41390-020-0789-6
更新日期：2020-10-01 00:00:00
abstract：:A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in serum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-197707000-00006
更新日期：1977-07-01 00:00:00
abstract：:Opsonisation of heat-killed bakers yeast and C3 and C4 concentrations were determined in sera from 69 children with liver disease and 39 controls as simple screening procedures for abnormalities in the complement system. Where significant defects in these moieties were encountered, the activity of the following was me...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198304000-00014
更新日期：1983-04-01 00:00:00
abstract：:The dipeptides carnosine and anserine, found exclusively in meats, are hydrolyzed in serum by the enzyme carnosinase. Several reports of serum carnosinase deficiency describe a variable phenotype, which ranges from normal to severe psychomotor retardation, hypotonia, and myoclonic seizures in the first year of life. W...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199702000-00009
更新日期：1997-02-01 00:00:00
abstract：:The association between lung injury and thrombocytopenia was investigated by comparing the megakaryocyte and platelet counts, and platelet activation using P-selectin as a marker, between the prepulmonary (right atrial) and postpulmonary (left atrial) blood in adult and neonatal (preterm and term) rats with and withou...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/01.PDR.0000079186.86219.29
更新日期：2003-09-01 00:00:00
abstract：:We investigated whether obese children and adolescents have early echocardiographic signs of subclinical cardiac dysfunction and evaluated the respective influence of obesity per se versus parameters of carbohydrate and lipid metabolism that are frequently abnormal in obese subjects. The role of tissue Doppler imaging...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/PDR.0b013e318176182b
更新日期：2008-08-01 00:00:00
abstract：:We examined the long-term effects of dietary protein restriction during rat pregnancy on serum IGF-I, serum IGF binding proteins, and liver IGF-I gene expression during postnatal development. Pregnant Wistar rats were fed ad libitum throughout gestation a normal (20% casein diet; P20 controls) or a low (5% casein; P5)...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199604000-00015
更新日期：1996-04-01 00:00:00
abstract：:The proposed actions of atrial natriuretic factor (ANF) are mediated through specific plasma membrane (R1) receptors coupled to guanylate cyclase. A second receptor, R2, has been characterized by its ability to bind to an acyclic, truncated ANF analog (C-ANF4-23). The ANF-R2 receptor has not been identified in the fet...
journal_title：Pediatric research
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：INTRODUCTION:Infection is a risk factor for adverse neurodevelopmental outcome in preterm newborns. Our objective was to characterize the association of postnatal infection with adverse microstructural and metabolic brain development in premature newborns. RESULTS:In 34/117 newborns studied, clinical signs were accomp...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1038/pr.2011.40
更新日期：2012-03-01 00:00:00
abstract：:To define respiratory effects of peripheral acidemia during early development, we injected lactic acid (LA) into the jugular vein of 32 1- to 7-d-old rabbit pups and four adult rabbits. The body weight of newborns varied between 67 +/- 17 g in 1-d-old and 119.8 +/- 33.7 g in 7-d-old animals versus 4 +/- 0.1 kg in adul...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199106010-00006
更新日期：1991-06-01 00:00:00
abstract：:This study investigated the effects of intraventricular/periventricular blood on cerebral cAMP production and cortical cerebrovascular reactivity. Under halothane and N2O anesthesia, 3 mL of either autologous blood or artificial cerebrospinal fluid (CSF) were injected into the left caudate nucleus; volume was adequate...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199709000-00010
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:No study has examined the association between prenatal phthalate exposure and intrauterine growth retardation (IUGR). This study aimed to investigate whether prenatal exposure to phthalates was associated with increased risk of IUGR. METHODS:A total of 126 mother-newborn pairs, including 42 IUGR cases and 8...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1038/pr.2014.103
更新日期：2014-10-01 00:00:00
abstract：:The effect of diet, human milk or formula, on gastric function (lipase and pepsin activity, pH, and volume) and intragastric digestion of fat was assessed in 28 appropriate for gestational age preterm infants (gestational age, 28.9 +/- 1.4, 29.1 +/- 0.9, 29.5 +/- 0.6 wk; birth weight, 1.00 +/- 0.14 to 1.18 +/- 0.07 kg...
journal_title：Pediatric research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1203/00006450-199609000-00011
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:Heterozygous ATP-binding-cassette subfamily A member 3 (ABCA3) mutations are associated with neonatal respiratory complications. In an adult murine model, we investigated whether Abca3 haploinsufficiency is a predisposing factor for lung injury induced by hyperoxia or mechanical ventilation. METHODS:Abca3 h...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1038/pr.2013.127
更新日期：2013-10-01 00:00:00
abstract：:The effects on the conceptus of persistently decreased maternal plasma amino acid concentrations were studied in pregnant rats by the infusion of glucagon (0.21 mg/day) to the mother from day 14 to 20 of gestation with a subcutaneous, osmotically driven minipump. Controls received diluent. The experimental animals eit...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198611000-00002
更新日期：1986-11-01 00:00:00
abstract：:Respiratory parameters of skeletal muscle were determined in permeabilized muscle fibers by adapting a technique described by Veksler et al. for cardiac fibers (Biochim Biophys Acta, 892:191-196, 1987). This method consists of the permeabilization of muscle fibers by saponin by allowing respiratory substrates and inhi...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199207000-00004
更新日期：1992-07-01 00:00:00
abstract：:We assessed the kinetics of hyperoxia-induced prostaglandin E2 (PGE2) production by cultured rabbit tracheal epithelial (TE) cells with different inherent capacities to generate PGE2 and the role of endogenous PGE2 production in protecting these cells from hyperoxic injury. Rabbit TE cells grown to confluence with or ...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199207000-00017
更新日期：1992-07-01 00:00:00
abstract：:Neuroblastoma, a neural crest-derived childhood tumor of the sympathetic nervous system, may in some cases differentiate to a benign ganglioneuroma or regress due to apoptosis. However, the majority of neuroblastomas are diagnosed as metastatic tumors with a poor prognosis despite intensive multimodal therapy. The neu...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199909000-00014
更新日期：1999-09-01 00:00:00
abstract：:Breast-fed infants have higher bilirubin levels than formula-fed infants, possibly because of variations in the composition of the breast milk. The aim of this study was to investigate whether there is a relationship between cytokine levels in the colostrum of nursing mothers and neonatal jaundice (NJ). Breast milk sa...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/PDR.0b013e31809871c9
更新日期：2007-08-01 00:00:00
abstract：:A child diagnosed at birth as deficient in red blood cell adenosine deaminase (ADA) but with substantial residual lymphocyte ADA has been evaluated for two and one-half years. The only immunologic abnormality observed was hypogammaglobulinemia during the fifth month of life. This was unexpected because children with t...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198007000-00009
更新日期：1980-07-01 00:00:00
abstract：:Several markers of growth and biochemical development in the rat were studied after administration of prostacyclin (PGI2) and 16, 16-dimethyl prostaglandin E2 (16, 16DM PGE2). Intermittent administration of PGI2 for 3 days to 10- and 19-day-old animals, with subsequent sacrifice at 14 and 23 days, resulted in signific...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198307000-00004
更新日期：1983-07-01 00:00:00
abstract：:Escherichia coli which elaborate heat stable enterotoxin (ST) are a major cause of endemic diarrhea in infants. The reason(s) for this increased susceptibility of infants to ST-mediated diarrhea is unknown. We investigated the possibility that the immature (14 and 21 day old) rat small intestine is more sensitive to S...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198606000-00017
更新日期：1986-06-01 00:00:00
abstract：:This report describes a third mucopolysaccharidosis in animals: canine mucopolysaccharidosis VII. The affected dog was the offspring of a father-daughter mating. Weakness in the rear legs was evident at 8 weeks of age and became progressively worse. He had a large head, a shortened maxilla, and corneal granularities. ...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198410000-00014
更新日期：1984-10-01 00:00:00
abstract：:Epidermal growth factor (EGF), a potent mitogen present in milk, is postulated to play an important physiologic role in the ontogeny of the intestine. An artificial rat milk, free of EGF, was developed and fed to newborn rats in an effort to evaluate the physiologic role of EGF on intestinal glucose transport. Compari...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198704000-00017
更新日期：1987-04-01 00:00:00
abstract：:In studies in the newborn infant, it is often assumed that there are similarities in airflow in successive breaths, and, therefore, it is only necessary to measure parameters in a small number of breaths. However, other studies have shown considerable variability in breathing patterns in successive breaths. It was, th...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-200007000-00011
更新日期：2000-07-01 00:00:00
abstract：:We studied the effects of anemia and ouabain administration on cardiac function, oxygen physiology, and blood catecholamine levels in nine newborn lambs. We measured oxygen consumption continuously, along with traditional hemodynamic variables. Oxygen transport was calculated. Following baseline measurements, lambs we...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198705000-00005
更新日期：1987-05-01 00:00:00
abstract：:Spleens from 1-20-wk-old guinea pigs infected in utero with Treponema pallidum and age-matched controls, born to normal and heat-killed (56 degrees C, 2 h.) T. pallidum-injected mothers, were examined for their in vitro lymphoproliferative response to phytohemagglutinin, concanavalin A, and lipopolysaccharide. Additio...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199712000-00013
更新日期：1997-12-01 00:00:00
abstract：:A four-year-old Israeli Arab boy was found to have glycogen accumulation in both liver and muscle without clinical symptoms. Liver phosphorylase kinase (PK) activity was 20% of normal, resulting in undetectable activity of phosphorylase a. Muscle PK activity was about 25% of normal, resulting in a marked decrease of p...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-198104000-00002
更新日期：1981-04-01 00:00:00
abstract：:The ontogeny of glutamine uptake by jejunal brush border membrane vesicles was studied in suckling and weanling rats and compared with the data obtained from previous studies done on adult rats in our laboratory. Glutamine uptake represented transport into the intravesicular space rather than mere binding into the mem...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199005000-00021
更新日期：1990-05-01 00:00:00
abstract：:Previous studies in mature fetal sheep have shown that alcohol depresses cerebral blood flow (CBF), cerebral O2 consumption (CMRO2), and cerebral glucose consumption (CMRglu). This effect earlier in gestation might contribute to the pathogenesis of fetal alcohol syndrome. Physiologic studies of immature fetal sheep ha...
journal_title：Pediatric research
pub_type： 杂志文章
doi：10.1203/00006450-199206000-00021
更新日期：1992-06-01 00:00:00
