Abstract：
PURPOSE:Silencing of HPV oncogenes or their human host client proteins using topically applied small interfering RNA (siRNA) may be an attractive nonsurgical strategy for CIN treatment. An exploratory clinical investigation was designed to evaluate E6-AP mRNA expression levels in different stages of cervical intraepithelial neoplasia and during the menstrual cycle. METHODS:In 38 premenopausal women aged 18-45 years referred to colposcopy clinic, analysis of serum hormones, cervical smears for cytology and HPV DNA, cervical biopsy, p16 immunohistochemistry and E6-AP mRNA expression levels in cervical smears and biopsies were performed. The intra-subject variability in E6-AP mRNA expression of vaginal smears was assessed and compared to cervical biopsy specimens. RESULTS:RNA of sufficient quantity and quality was available for E6-AP expression analysis from 97 % of the collected cervical smears and from 56 % of the collected biopsy samples. The normalized RNA levels from cervical smears were approximately tenfold higher compared to biopsies. There was little influence by the phase of the menstrual cycle or by CIN stage. Real-time PCR showed that the expression level of E6-AP is in a range (<28 C t) that would allow for detection of at least 100-fold modulation by a therapeutic agent (based on an assay LOD of C t = 36). CONCLUSIONS:Our findings suggest a potential therapeutic approach using E6-AP siRNA as a specific molecular target in cervical intraepithelial neoplasia.
journal_name
Arch Gynecol Obstetjournal_title
Archives of gynecology and obstetricsauthors
Hillemanns P,Jentschke M,Evans TG,Soergel P,Hass Rdoi
10.1007/s00404-013-3120-5subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1281-6issue
6eissn
0932-0067issn
1432-0711journal_volume
289pub_type
杂志文章abstract：AIM:To report an unusual location of a transmigrated IUD which become embedded in the right ovary causing chronic pelvic pain and dyspareunia. CASE:A 22-year-old woman who had an IUD (Copper T), inserted 7 years ago presented with complaint of lower abdominal pain. Pelvic ultrasonographic examination revealed ovarian ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-008-0882-2
更新日期：2009-08-01 00:00:00
abstract：BACKGROUND:Cerebral malformations may lead to permanent postnatal sequels. The antenatal detection of anomalous or absent fetal sulci and gyri may indicate abnormal brain development and future neurological and psychomotor problems in that infant. The prenatal diagnosis of these conditions allows genetic counseling, ps...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-010-1691-y
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:Atosiban has been shown to be an effective tocolytic agent with a low rate of side effects during 24 to 33 weeks of gestation. Atosiban acts through selective, competitive inhibition of both oxytocin and vasopressin, so that there are reasons to assume that a tocolytic effect can also be achieved earlier in t...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00404-004-0652-8
更新日期：2005-06-01 00:00:00
abstract：:Between 1989 and 1994 first trimester transcervical chorionic villus sampling was offered to 158 patients after proper counselling. The gestational age range was 9-12 weeks. The most important indication was advanced maternal age (75.4%). Adequate sampling after two, and a maximum of three attempts, was achieved in 98...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/BF02505315
更新日期：1997-01-01 00:00:00
abstract：OBJECTIVE:The objective of this research was to identify the rate of unexpected malignancies after laparoscopic-assisted supracervical hysterectomies (LASH) and describe the therapy regime. METHODS:The research is based on a retrospective chart analysis of patients undergoing a simple hysterectomy in the gynecological...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-012-2559-0
更新日期：2013-03-01 00:00:00
abstract：PURPOSE:We aimed to investigate the impact of early versus late discharge following cesarean delivery (CD) on re-admission rate. METHODS:This is a retrospective cohort study of all CDs performed between 2013 and 2016. Patients who underwent CD between 01/13 and 12/14, were routinely discharged on postoperative day (PO...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-018-4828-z
更新日期：2018-09-01 00:00:00
abstract：PURPOSE:In case of non-gynecological solid tumors and hematological malignancies diagnosed during pregnancy, individual diagnostic and treatment options must be established by an interdisciplinary team. METHODS:In part II of the present review we report on diagnostic and therapeutic principles in distinct entities of ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-011-2059-7
更新日期：2011-12-01 00:00:00
abstract：PURPOSE:Giant oocytes are potential sources of chromosomal abnormalities and should thus never be used in in vitro fertilization and embryo transfer (IVF-ET) procedures. The presence of giant oocytes may indicate the efficiency of the ovarian stimulation and can refer to the quality of sibling oocytes. METHODS:IVF cyc...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-015-3679-0
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:Case report of a rare combination of a trisomy 18 and 21 in a dizygotic twin pregnancy in a woman with a history of recurrent miscarriage, a neonatal death, no living offspring and Graves disease. METHODS:Case report and literature search. RESULTS:Only one other report in the literature of a combined trisom...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-007-0378-5
更新日期：2007-11-01 00:00:00
abstract：:Intrauterine retention of foetal bones is an uncommon but recognised complication of late termination of pregnancy. Secondary subfertility, abnormal uterine bleeding and vaginal discharge are the usual presenting complaints. We report a case of prolonged retention of foetal bones for 14 years in a woman who presented ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-008-0686-4
更新日期：2009-02-01 00:00:00
abstract：:Tubal sterilization is one of the options chosen by the women who have completed their fertility. Although ectopic pregnancy after tubal sterilization is rare, all women undergoing this procedure should be informed about the risk of it. An ectopic pregnancy in a 36-year-old woman who had undergone tubal sterilization ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/pl00007502
更新日期：2002-01-01 00:00:00
abstract：PURPOSE:The objective of this study was to determine the changes in total plasma thiols (homocysteine, cysteine and cysteinylglycine), lipid peroxidation and nitric oxide concentrations during normal pregnancy. METHODS:These variables were measured in 28 uncomplicated pregnant women at first, second and third trimeste...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-011-2150-0
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVE:To investigate whether episiotomy prevents 3rd or 4th degree perineal tears in critical conditions such as shoulder dystocia, instrumental deliveries (vacuum or forceps), persistent occiput-posterior position, fetal macrosomia (>4,000 g), and non-reassuring fetal heart rate (NRFHR) patterns. METHODS:A retros...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-012-2460-x
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the effect of two different doses of dexpanthenol (Dxp) onexperimentally induced ovarian ischaemia/reperfusion (I/R) injury ina rat model. STUDY DESIGN:Forty female rats were randomly divided into fivegroups: Group 1: sham operation; Group 2: 3-h ischaemia; Groups 3: 3-h...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-017-4287-y
更新日期：2017-03-01 00:00:00
abstract：PURPOSE:Women with previous gestational diabetes mellitus (pGDM) are at high risk for type 2 diabetes and cardiovascular disorders. In this study, we aimed to compare plasma apelin levels between women with and without pGDM, and to investigate the possible association of apelin with cardiometabolic risk factors. METHO...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-013-3070-y
更新日期：2014-04-01 00:00:00
abstract：METHODS:Twenty-six triplet pregnancies were delivered over 6 years. RESULTS:Over 80% of pregnancies resulted after infertility treatment (21/26). Mean maternal age was 32. On average, the infants were delivered after 32 + 0 weeks; in 4 cases delivery was at <28 weeks. In 1 case, the leading triplet was delivered spont...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-009-0929-z
更新日期：2009-04-01 00:00:00
abstract：:Recent studies described the presence of elastic fibres within stem villi of human term placenta. This particular study focuses on changes of the elastic fibre system in placental villous tree from placentas of different gestational ages. The presence of elastic fibres was researched by light and electron microscopy i...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-002-0314-7
更新日期：2003-02-01 00:00:00
abstract：BACKGROUND:Uterine epithelioid angiosarcoma is extremely rare. Evidence-based advice regarding optimal management is lacking. METHOD:We report the 22nd case in the world literature and review all the cases reported in English since 1900. An attempt is made to generate information that may help in the management based ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章,评审
doi：10.1007/s00404-008-0727-z
更新日期：2008-11-01 00:00:00
abstract：INTRODUCTION:The high incidence of multiple pregnancies is a major concern in the treatment of infertility by in vitro fertilization (IVF). Risks and costs are associated with multiple pregnancies and it is important to find a way to decrease multiple pregnancies. The standard protocol for IVF includes the transfer of ...
journal_title：Archives of gynecology and obstetrics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00404-003-0513-x
更新日期：2004-09-01 00:00:00
abstract：:We retrospectively reviewed the perinatal outcome of twin pregnancies cohere the first baby was presenting by the breech. 21 were delivered vaginally and 37 abdominally. Differences in perinatal outcome, as measured by Apgar score and mortality, were not apparently different. ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s004040050201
更新日期：1998-01-01 00:00:00
abstract：:We report a 34-year-old woman who presented at 21 weeks with cardiac failure due to idiopathic dilated cardiomyopathy. Following refusal of pregnancy termination, she was treated conservatively with good maternal and neonatal outcome. ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/BF02390676
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVES:We analyze the incidence, etiology, outcome of pregnancy and therapeutic regimes of prenatally and postnatally detected isolated and complex congenital talipes equinovarus in a tertiary referral center. METHODS:We included fetuses with at least one prenatal ultrasound examination conducted by a sub-speciali...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-012-2325-3
更新日期：2012-10-01 00:00:00
abstract：PURPOSE:Human papillomavirus (HPV) can cause condylomata acuminata, also known as genital warts. Our aim was to evaluate the long-term recurrence of genital warts after primary carbon dioxide laser treatment before the introduction of the vaccination against HPV. METHODS:Recurrence rate and localization of genital war...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-019-05242-5
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:Both cigarette smoking and alcohol consumption are somehow implicated in sperm function, but the impact of these two lifestyle factors on sperm parameters remains controversial. The present study is focused on the impact of cigarette smoking and alcohol consumption separately and combined on sperm parameters an...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-014-3281-x
更新日期：2014-10-01 00:00:00
abstract：:4 patients with recurrent gynecological malignancy were treated photodynamically, 4 d after sensitisation with intravenous meso-tetra(hydroxyphenyl)-chlorin (m-THPC) at a dose of 0.15 mg/kg bodyweight (total dose ranged from 12-15 mg). Light at 652 nm was derived from a KTP-Dye laser (Diasonic) and delivered superfici...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s004040050249
更新日期：1999-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to investigate the prevalence of human papillomavirus (HPV) genotypes infection in women with and with normal cervical histology in the littoral region of Shandong, China. METHODS:Scrape specimens were collected from 4,601 women for Pap smear and HPV genotypes identification. 1,...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-010-1584-0
更新日期：2011-06-01 00:00:00
abstract：INTRODUCTION:Intentional placental removal for abnormally invasive placenta (AIP) is fundamentally abandoned at planned surgery for it. Whether this holds true even after recent introduction of various hemostatic procedures is unclear. MATERIALS AND METHODS:We discussed on this issue based on our own experiences and a...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-017-4592-5
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:To determine a predictive model for supporting decisions relating to the prognosis for women presenting with preterm labor and intact membranes. METHOD:We conducted a prospective observational cohort study on 70 pregnant women at between 22 and 34 weeks of gestation. Transvaginal sonographic evaluation on the ...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-012-2397-0
更新日期：2012-10-01 00:00:00
abstract：PURPOSE:To ascertain any differences in foetomaternal outcomes in induced and spontaneous labour among nulliparous women delivering at term. METHODS:A retrospective matched cohort study consisting of 403 nulliparous women induced at ≥ 292 days and 806 nulliparous women with spontaneous labour at 285-291 days. RESULTS...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-010-1671-2
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To identify differentially expressed genes (DEGs) in endometriosis and further analyze molecular mechanisms implicated in disease pathogenesis. MATERIALS AND METHODS:Gene expression data (ID: GSE7846) of human endometrial endothelial cells (HEECs) collected from eutopic endometria tissue of patients with and...
journal_title：Archives of gynecology and obstetrics
pub_type： 杂志文章
doi：10.1007/s00404-015-3875-y
更新日期：2016-04-01 00:00:00
