Lactobacillus rhamnosus GG on rotavirus-induced injury of ileal epithelium in gnotobiotic pigs.

Abstract:

OBJECTIVE:The aim of this study was to study the effect of continued Lactobacillus rhamnosus GG strain (LGG) feeding on rotavirus gastroenteritis in the gnotobiotic (Gn) pig model of virulent human rotavirus (HRV) infection. METHODS:Gn pigs were assigned to treatment groups: mock control, LGG only, HRV only, or LGG plus HRV. Nine days before HRV inoculation (3 days of age), pigs were fed LGG with a daily dose increase of 10-fold from 10³ to 10¹² colony-forming units (CFU). The 10¹² CFU/dose of LGG feeding continued until post-HRV inoculation day (PID) 6. Clinical sign (diarrhea), rotavirus fecal shedding, histopathology of the ileum, adherent junction and tight junction protein expression in the ileal epithelial cells, mucin production in the large and small intestinal contents, and serum cytokine responses from PID 2 to 6 were examined and compared among the treatment groups. RESULTS:Clinically, the percentage of pigs developing diarrhea, the mean duration of diarrhea, and the mean cumulative fecal scores were lower in the LGG fed pigs compared to the nonfed pigs after HRV inoculation. LGG partially protected ileal epithelium against HRV-induced compensatory increases of the adherent junction protein α-catenin and β-catenin, tight junction protein occludin, claudin-3 and claudin-4, and leak protein claudin-2. LGG promoted mucin production because the mucin levels in the large intestinal contents of the LGG+HRV pigs were significantly higher than the HRV-only pigs on PID 2. Additionally, LGG maintained the anti-inflammatory cytokine transforming growth factor-β level in serum after HRV infection. CONCLUSIONS:LGG is moderately effective for ameliorating rotavirus diarrhea by partially preventing injuries to the epithelium.

authors

Liu F,Li G,Wen K,Wu S,Zhang Y,Bui T,Yang X,Kocher J,Sun J,Jortner B,Yuan L

doi

10.1097/MPG.0b013e3182a356e1

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

750-8

issue

6

eissn

0277-2116

issn

1536-4801

pii

00005176-201312000-00013

journal_volume

57

pub_type

杂志文章
  • Outcomes of Severe Seronegative Hepatitis-associated Aplastic Anemia: A Pediatric Case Series.

    abstract:OBJECTIVES:Hepatitis-associated aplastic anemia (HAAA) is a potentially life-threatening diagnosis without clear treatment guidelines. The goal of the study was to characterize the presentation, evaluation, histopathology, and outcomes of therapy in children with HAAA to guide future research and to develop standardize...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000002940

    authors: Kemme S,Stahl M,Brigham D,Lovell MA,Nakano T,Feldman AG,Mack C

    更新日期:2021-02-01 00:00:00

  • The relationship between the concentration of dextran sodium sulfate and the degree of induced experimental colitis in weanling rats.

    abstract:BACKGROUND:Although a dextran sodium sulfate (DSS)-induced colitis is commonly used as an ulcerative colitis (UC) model in adult rodents, there are no studies using this model in young animals. We examined differences in the severity of DSS-induced colitis as a function of the concentration of DSS administered and soug...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-200310000-00015

    authors: Shimizu T,Suzuki M,Fujimura J,Hisada K,Yoshikazu O,Obinata K,Yamashiro Y

    更新日期:2003-10-01 00:00:00

  • Childhood Bladder and Bowel Dysfunction Questionnaire: Development, Feasibility, and Aspects of Validity and Reliability.

    abstract:OBJECTIVES:The aim of the study was to develop a questionnaire evaluating the frequency of symptoms over time of concomitant childhood bladder and bowel dysfunctions (CBBDs) in 5- to 12-year-old children and to assess its feasibility and aspects of validity and reliability. METHODS:The Childhood Bladder and Bowel Dysf...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000001461

    authors: van Engelenburg-van Lonkhuyzen ML,Bols EMJ,Bastiaenen CHG,Benninga MA,de Bie RA

    更新日期:2017-06-01 00:00:00

  • Growth in Infants and Children With Intestinal Failure-associated Liver Disease Treated With Intravenous Fish Oil.

    abstract:BACKGROUND:Infants with intestinal failure (IF) and IF-associated liver disease (IFALD) are at risk for poor somatic growth because of increased metabolic demands, inadequate intake, intestinal malabsorption, chronic liver disease and other comorbidities. There are limited data on the nutritional adequacy of intravenou...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000002551

    authors: Raphael BP,Mitchell PD,Gura KM,Potemkin AK,Squires RH,Puder M,Duggan CP

    更新日期:2020-02-01 00:00:00

  • Breath hydrogen analysis: a review of the methodologies and clinical applications.

    abstract::Hydrogen gas (H2) is a product of the fermentation of dietary carbohydrate (CHO) by bacteria in the lumen of the gastrointestinal tract in man. Thus, H2 is actually an exogenously produced gas, which either is passed as flatus, or diffuses into the body and is exhaled. In the adult, a fairly constant fraction is expir...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:

    authors: Ostrander CR,Cohen RS,Hopper AO,Shahin SM,Kerner JA Jr,Johnson JD,Stevenson DK

    更新日期:1983-01-01 00:00:00

  • Management of cyclic vomiting syndrome.

    abstract::The general goals of treatment of cyclic vomiting syndrome (CVS) are: interruption of established episodes, amelioration of symptoms in patients whose episodes cannot be interrupted, aborting episodes during prodromal symptoms, prophylaxis to abolish or lessen the frequency of episodes, and recovery. Complications of ...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章,评审

    doi:10.1097/00005176-199501001-00015

    authors: Fleisher DR

    更新日期:1995-01-01 00:00:00

  • Hepatic abscess as a complication of Crohn's disease.

    abstract::A 17-year-old male with Crohn's disease presented with persistent fever and right upper quadrant tenderness. Ultrasonography and abdominal computerized axial tomography (CAT scan) showed two large loculated abscess cavities in the right lobe of the liver. Surgical drainage and antibiotic therapy led to resolution of b...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-198302010-00021

    authors: Weinberg RJ,Klish WJ,Brown MR,Smalley JR,Emmens RW

    更新日期:1983-01-01 00:00:00

  • Update on progressive familial intrahepatic cholestasis.

    abstract::Three distinct forms of familial intrahepatic cholestasis are the result of mutations in the ATP8B1, ABCB11, and ABCB4 genes. The pathophysiologies of the latter 2 of these diseases are well characterized and are the result of abnormalities in canalicular excretion of bile acids and phospholipids, respectively. The mo...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章,评审

    doi:10.1097/MPG.0b013e3181596060

    authors: Alissa FT,Jaffe R,Shneider BL

    更新日期:2008-03-01 00:00:00

  • Follow-up after pH-metry and pH impedance in pediatric gastroesophageal reflux disease.

    abstract:OBJECTIVES:It is unclear how diagnostic tests for gastroesophageal reflux disease (GERD) in children relate to the course of symptoms during follow-up. METHODS:During 1 year, all children (ages 0-18 years), who underwent pH-metry and/or pH multichannel intraluminal impedance (MII), were included after written informed...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000000582

    authors: van der Pol RJ,van den Ouweland M,Loots CM,Vandenplas Y,Benninga MA,van Wijk MP

    更新日期:2015-02-01 00:00:00

  • The influence of gestational age, size for dates, and prenatal steroids on cord transferrin levels in newborn infants.

    abstract::Serum transferrin levels assess protein status in older children and adults. To generate standards for its use in newborn infants, we measured umbilical cord serum transferrin levels in 161 appropriate (AGA), 25 large (LGA) and 16 small (SGA) for gestational age infants between 25 and 43 weeks' gestation. We also asse...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-198703000-00020

    authors: Chockalingam U,Murphy E,Ophoven JC,Georgieff MK

    更新日期:1987-03-01 00:00:00

  • Analysis of Using the Total White Blood Cell Count to Define Severe New-onset Ulcerative Colitis in Children.

    abstract:OBJECTIVES:The aim of this study was to assess common laboratory tests in identifying severe ulcerative colitis in children at diagnosis. METHODS:A cohort of 427 children 4 to 17 years of age newly diagnosed with ulcerative colitis (UC) was prospectively enrolled. Boosted classification trees were used to characterize...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000002797

    authors: Mack DR,Saul B,Boyle B,Griffiths A,Sauer C,Markowitz J,LeLeiko N,Keljo D,Rosh JR,Baker SS,Steiner S,Heyman MB,Patel AS,Baldassano R,Noe J,Rufo P,Kugathasan S,Walters T,Marquis A,Thomas SM,Denson L,Hyams J,PROT

    更新日期:2020-09-01 00:00:00

  • Recommendations for Diagnosis and Management of Autoimmune Pancreatitis in Childhood: Consensus From INSPPIRE.

    abstract:OBJECTIVES:Autoimmune pancreatitis (AIP) represents a complex immune-mediated pancreas disorder. Pediatric AIP (P-AIP) is rare. We have recently summarized the characteristic features of P-AIP. We now aim to develop recommendation statements to standardize the diagnostic and therapeutic approach to P-AIP and facilitate...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 共识发展会议,杂志文章,实务指引

    doi:10.1097/MPG.0000000000002028

    authors: Scheers I,Palermo JJ,Freedman S,Wilschanski M,Shah U,Abu-El-Haija M,Barth B,Fishman DS,Gariepy C,Giefer MJ,Heyman MB,Himes RW,Husain SZ,Lin TK,Liu Q,Lowe M,Mascarenhas M,Morinville V,Ooi CY,Perito ER,Piccoli DA,

    更新日期:2018-08-01 00:00:00

  • Adverse Events Following Gastrointestinal Endoscopy in Children: Classifications, Characterizations, and Implications.

    abstract:OBJECTIVE:To conduct a comprehensive study of postendoscopic adverse events (AEs) in children, categorizing them by the level of intervention required. BACKGROUND:Previous studies of endoscopic AE in children have focused on intraprocedural and short-term outcomes, such as bleeding, perforation, and infection, and may...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000001038

    authors: Kramer RE,Narkewicz MR

    更新日期:2016-06-01 00:00:00

  • Effects of loperamide on intestinal ion transport.

    abstract::The synthetic opiate loperamide (LPMD) is an antidiarrheal compound which affects both intestinal motility and the transport of water and electrolytes. We have investigated its effects on ion transport in the stripped ileal mucosa of rabbits, mounted in Ussing chambers in vitro. Addition of LPMD to serosal bathing med...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-198409000-00020

    authors: Guandalini S,Fasano A,Rao MC,Ferola A,Migliavacca M,Marchesano G,Rubino A

    更新日期:1984-09-01 00:00:00

  • New Algorithm for the Integration of Ultrasound Into Cystic Fibrosis Liver Disease Screening.

    abstract:OBJECTIVES:Liver nodularity occurs across the spectrum of cystic fibrosis liver disease (CFLD), from regenerative nodules to cirrhosis, and can occur without liver enzyme abnormalities. Our aims were to determine if incorporating abdominal ultrasound (US) with annual laboratory testing improves the detection of CFLD an...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000002412

    authors: Sellers ZM,Lee LW,Barth RA,Milla C

    更新日期:2019-10-01 00:00:00

  • Gastric emptying in children: what is the best acquisition method?

    abstract:OBJECTIVES:The geometric mean is the recommended method for calculating gastric emptying in adults to correct for variable attenuation during the study. In children, it has been reported that a single posterior acquisition is sufficient. Our objective was to evaluate the relation between the values of gastric emptying ...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0b013e31824ee2fa

    authors: Drubach LA,Kourmouzi V,Cao X,Zurakowski D,Fahey FH

    更新日期:2012-08-01 00:00:00

  • Pediatric NAFLD: the Future role of Patient-Tailored Probiotics Therapy.

    abstract:PURPOSE OF REVIEW:Clinical evidence of the use of probiotics in pediatric NAFLD, framed within gut microbiota response, is herein discussed. RECENT FINDINGS:The assistance of a new microbiological approach in probiotics' design is playing a central role in NAFLD clinical management. Experimental studies demonstrated t...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章,评审

    doi:10.1097/MPG.0000000000001220

    authors: Putignani L,Alisi A,Nobili V

    更新日期:2016-07-01 00:00:00

  • First salivary screening of celiac disease by detection of anti-transglutaminase autoantibody radioimmunoassay in 5000 Italian primary schoolchildren.

    abstract:OBJECTIVE:The high prevalence of celiac disease (CD) prompted us to evaluate a new, noninvasive disease screening strategy. The aim was to identify CD in 6- to 8-year-old children for a timely diagnosis, start gluten-free diet (GFD) in compliant subjects, achieve the growth target, and prevent CD complications. METHOD...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0b013e3181e6f2d0

    authors: Bonamico M,Nenna R,Montuori M,Luparia RP,Turchetti A,Mennini M,Lucantoni F,Masotti D,Magliocca FM,Culasso F,Tiberti C

    更新日期:2011-01-01 00:00:00

  • Economic Impact and Prognostic Factors of Functional Dyspepsia in Children.

    abstract:OBJECTIVES:Functional dyspepsia (FD) is a functional abdominal pain disorder. There is paucity of data on the economic impact of FD in children. Primary aim of our study was to estimate annual evaluation cost ("diagnosis and visit" cost) and secondary aim was to identify potential prognostic factors of FD in children. ...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000002599

    authors: Mani J,Madani S,Thomas R

    更新日期:2020-04-01 00:00:00

  • Pilot Study of Functional Magnetic Resonance Imaging Responses to Somatic Pain Stimuli in Youth With Functional and Inflammatory Gastrointestinal Disease.

    abstract:BACKGROUND:Brain-gut axis signaling modifies gastrointestinal symptomatology. Altered neural processing of intestinal pain signals involves interoceptive brain regions in adults with functional and inflammatory gastrointestinal disorders. Although these disorders frequently present in childhood, there are no published ...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000001390

    authors: Huang JS,Terrones L,Simmons AN,Kaye W,Strigo I

    更新日期:2016-11-01 00:00:00

  • A new model of chronic hapten-induced colitis in young rats.

    abstract:AIM AND OBJECTIVE:: Chronic models of inflammatory bowel disease are lacking in preadult rodents. The primary goal of our study was to develop a chronic model of hapten-induced intestinal inflammation and fibrosis in young rats. Second, we aimed to determine the profiles of key Th-1, Th-2, and Th-17 proinflammatory and...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0b013e3181cb8f4a

    authors: Fitzpatrick LR,Meirelles K,Small JS,Puleo FJ,Koltun WA,Cooney RN

    更新日期:2010-03-01 00:00:00

  • Frequency and clinical correlations of granulomas in children with Crohn disease.

    abstract:BACKGROUND:Granulomas are pathognomonic findings of Crohn disease (CD); however, their occurrence and clinical significance are not well characterized. Our aim was to determine the frequency and distribution of granulomas in untreated and treated patients with CD and their relation to age and disease severity. PATIENT...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0b013e31812e95e1

    authors: De Matos V,Russo PA,Cohen AB,Mamula P,Baldassano RN,Piccoli DA

    更新日期:2008-04-01 00:00:00

  • Sonographic evaluation of portal hypertension in children.

    abstract::Portal hypertension, an expected consequence of cirrhosis, often has an insidious course in children. A noninvasive technique using abdominal sonography has been previously employed by several investigators as a means of diagnosing this condition. Their technique involves sonographically measuring the diameter of the ...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-199004000-00021

    authors: Rabinowitz SS,Norton KI,Benkov KJ,Yeh HC,Leleiko NS

    更新日期:1990-04-01 00:00:00

  • Prolonged postprandial abdominal pain following Kawasaki syndrome with acute gallbladder hydrops: association with impaired gallbladder emptying.

    abstract::Acute hydrops of the gallbladder is a well-recognized complication of Kawasaki syndrome. We report a case of a child with this syndrome whose gallbladder hydrops slowly resolved after intravenous gamma-globulin therapy. However, he continued to experience postprandial right upper quadrant abdominal pain. Hepatobiliary...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-199110000-00013

    authors: Bishop WP,Kao SC

    更新日期:1991-10-01 00:00:00

  • Feeding Disorders in Children With Autism Spectrum Disorders Are Associated With Eosinophilic Esophagitis.

    abstract:OBJECTIVES:Eosinophilic esophagitis (EoE) can present as food selectivity or feeding disorders in children. Children with autism spectrum disorders (ASDs) commonly demonstrate behavioral food selectivity in type and texture, which often leads to the diagnosis of feeding disorder. We sought to evaluate the association o...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000001282

    authors: Heifert TA,Susi A,Hisle-Gorman E,Erdie-Lalena CR,Gorman G,Min SB,Nylund CM

    更新日期:2016-10-01 00:00:00

  • Nuclear pregnane X receptor single nucleotide polymorphism (-25385C/T) is not associated with inflammatory bowel disease in pediatric patients.

    abstract:OBJECTIVE:Studies in adults characterized the role of the pregnane X receptor (PXR) in the pathophysiology of inflammatory bowel disease (IBD) with conflicting results; pediatric studies are still lacking. PATIENTS AND METHODS:Genotyping for the -25385C/T polymorphism of the PXR gene in 187 white children with IBD and...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0b013e31818de1a8

    authors: Lacher M,Kappler R,Schroepf S,Berkholz S,Ballauff A,Bufler P,Baurecht H,von Schweinitz D,Koletzko S

    更新日期:2009-07-01 00:00:00

  • Herpes Simplex Virus Esophagitis in Immunocompetent Children: A Harbinger of Eosinophilic Esophagitis?

    abstract::Herpes simplex virus (HSV) is a common cause of infectious esophagitis. The aim of this retrospective study is to identify comorbid and predisposing conditions and sequelae of HSV esophagitis in immunocompetent children. We reviewed 16 cases of HSV esophagitis diagnosed from January 1982 to March 2016. Five patients w...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/MPG.0000000000001748

    authors: Fritz J,Lerner D,Suchi M

    更新日期:2018-04-01 00:00:00

  • Hyperosmolal formula in neonatal piglets: effects on gastrointestinal hormone concentrations, enteric bacterial titers, and small intestinal histology.

    abstract::Ingestion of hyperosmolal formula (HOF) by neonatal piglets has been shown to cause significant time-dependent reduction in phase 3 myoelectric activity, which persists in the terminal ileum. To determine whether a single hyperosmolal meal leads to elevated concentrations of gastrointestinal (GI) hormones that inhibit...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:

    authors: Szabo JS,Rayford PL,Uthman E,Lobe TE

    更新日期:1990-07-01 00:00:00

  • Epithelial growth of the small intestine in human infants.

    abstract:BACKGROUND:Findings in studies in rodents have suggested that epithelial growth of the small intestine is dependent on activation of the immune system. The purpose of this study was to compare changes of postnatal epithelial growth with immunologic activity in humans. METHODS:Duodenal biopsies were obtained by endosco...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-199805000-00004

    authors: Thompson FM,Catto-Smith AG,Moore D,Davidson G,Cummins AG

    更新日期:1998-05-01 00:00:00

  • Insulin-like growth factors and leucine kinetics during exercise training in children with cystic fibrosis.

    abstract:BACKGROUND:Little is known about the metabolic effects of exercise training in children with cystic fibrosis. The hypothesis for the current study was that in patients with declining clinical status, exercise increases circulating insulin-like growth factors (IGFs) and improves protein kinetics. METHODS:This was a pro...

    journal_title:Journal of pediatric gastroenterology and nutrition

    pub_type: 杂志文章

    doi:10.1097/00005176-200101000-00020

    authors: Gulmans V,van der Laag J,Wattimena D,van Doorn J,Oostveen D,Berger R,de Meer K

    更新日期:2001-01-01 00:00:00