Simultaneous isolation of Rutin and Esculin from plant material and drugs using solid-phase extraction.

abstract：:A RP-HPLC method was developed and validated for the degradation kinetic study of 2-(2-hydroxypropanamido) benzoic acid (HPABA), a promising anti-inflammatory drug, which would provide a basis for further studies on HPABA. The effects of pH, temperature, buffer concentration and ionic strength on the degradation kinet...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang Q,Guan J,Rong R,Zhao Y,Yu Z

更新日期：2015-08-10 00:00:00

abstract：:Obesity is one of the independent risk factors for several health problems, leading to metabolic perturbations and for which analytical approaches i.e., "metabolomics" is needed to monitor the underlying metabolic changes. In this study, obesity associated changes were assessed via serum metabolites analysis of obese ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ammar NM,Farag MA,Kholeif TE,Metwally NS,El-Sheikh NM,El Gendy AN,Abdel-Hamid AZ

更新日期：2017-08-05 00:00:00

abstract：:A simple HPLC method with UV detection is proposed for the simultaneous determination of three lipophilic vitamins: all-trans-retinol, alpha-tocopherol and coenzyme Q(10) (ubiquinone) in human plasma. The following chromatographic conditions were used: RP-18 column, a mobile phase consisted of methanol -n-hexane 72:28...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Karpińska J,Mikołuć B,Motkowski R,Piotrowska-Jastrzebska J

更新日期：2006-09-18 00:00:00

abstract：:An analytical method was developed for the determination of the enantiomers of felodipine, a dihydropyridine-type calcium antagonist, in human blood plasma. Felodipine was extracted from plasma using toluene as extraction solvent. The enantiomers were separated on a cellulose tris(4-methyl benzoate) stationary phase (...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Lindmark B,Ahnoff M,Persson BA

更新日期：2002-01-15 00:00:00

abstract：:Degradation of heparan sulfate proteoglycan by heparanase is an important process in tissue invasion by malignant tumor cells and inflammatory cells. Several heparanase assays have been reported previously, but all of them are cumbersome and time-consuming for separating degradative products from uncleaved substrates ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Enomoto K,Okamoto H,Numata Y,Takemoto H

更新日期：2006-06-07 00:00:00

abstract：:Macrolides are multicomponent drugs whose impurity control is always a challenge demanding analysis method with good sensitivity and selectivity. Three separate, sensitive, accurate liquid chromatography tandem mass spectrometry methods (LC-MS) were developed for the measurement of three 16-membered ring macrolides (j...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang X,Li J,Wang C,Song D,Hu C

更新日期：2017-10-25 00:00:00

abstract：:Fentanyl, a potent analgesic drug, has traditionally been used intravenously in surgical or diagnostic operations. Formulations with fentanyl in oral transmucosal delivery system and in transdermal depot-patch have also been developed against breakthrough pain in cancer patients. In this report, LC-MS/MS methods to de...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Huynh NH,Tyrefors N,Ekman L,Johansson M

更新日期：2005-04-29 00:00:00

abstract：:Cocaine is one of the most worldwide used illicit drugs. We report a magnetic particles-based enzyme-linked immunoassay (mpEIA) method for the rapid and sensitive determination of cocaine (COC) in saliva, urine and serum samples. Under optimized conditions, the limits of detections were 0.09ngmL(-1) (urine), 0.15ngmL(...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Vidal JC,Bertolín JR,Bonel L,Asturias L,Arcos-Martínez MJ,Castillo JR

更新日期：2016-06-05 00:00:00

abstract：:Twenty-seven highly diversified pharmaceutical compounds were used as a test set to evaluate matrix effects from microsomal media on LC/MS analyses. The individual effects of Tris buffer, NADPH and microsomes on ESI response were investigated. Direct flow injection MS/MS analysis, using no sample preparation or HPLC s...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zheng JJ,Lynch ED,Unger SE

更新日期：2002-04-15 00:00:00

abstract：:PGE-9509924 is a nonfluorinated quinolone and is active against a variety of susceptible and drug resistant bacteria in vitro and in animal infection models. A method for determining both enantiomers of PGE-9509924 in dog plasma has been developed. The enantiomers are derivatized with a chiral derivatizing agent, (-)-...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zoutendam PH,Canty JF,Martin MJ,Dirr MK

更新日期：2002-08-22 00:00:00

abstract：:This work presents an evaluation of the analytical performance of three different portable near-infrared (NIR) instruments (denominated Port.1, Port.2 and Port.3) for quantifying mebendazole polymorphs (A, B and C) in pharmaceutical raw materials using multivariate calibration models. The performance of the portable i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： da Silva VH,da Silva JJ,Pereira CF

更新日期：2017-02-05 00:00:00

abstract：:Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESIMSn) was employed to investigate the in vivo and in vitro metabolism of anisodine. Feces, urine and plasma samples were collected after ingestion of 20 mg anisodine to healthy rats. Feces and urine samples were cleaned up by liquid-liquid ex...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Chen H,Chen Y,Du P,Han F

更新日期：2007-07-27 00:00:00

abstract：:A method for the analysis of aqueous samples of isosorbide-5-mononitrate (5-ISMN) is presented. It is based on packed column supercritical fluid chromatography (SFC) using 20% of 2-propanol in carbon dioxide as the mobile phase and a diol silica column as the stationary phase. Using the described conditions it is poss...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gyllenhaal O,Hulthe J

更新日期：2002-06-20 00:00:00

abstract：:Ionic liquid-based salt-induced liquid-liquid extraction was developed for the first time and applied to the extraction of four active constituents, including polydatin, resveratrol, emodin, and physcion in Polygonum cuspidatum (P. cuspidatum). In this study, ionic liquid was used as extraction solvent. The dried P. c...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wang J,Feng J,Xu L,Ma J,Li J,Ma R,Sun K,Wang Z,Zhang H

更新日期：2019-01-30 00:00:00

abstract：:In this paper the development and validation of a method for the analysis of THC-COOH, THC, THC-OH, CBD and CBN in their total form in urine by LC-MS/MS is presented. Tandem hydrolysis, i.e. enzymatic and basic, has been found optimal for the simultaneous analysis of the selected analytes in urine: basic hydrolysis is...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Montesano C,Sergi M,Odoardi S,Simeoni MC,Compagnone D,Curini R

更新日期：2014-03-01 00:00:00

abstract：:Stable isotope labeled (SIL) compounds have been commonly used as internal standards (IS) to ensure the accuracy and quality of liquid chromatography-mass spectrometry (LC-MS) bioanalytical assays. Recently, the application of SIL drugs and LC-MS assays to microdose absolute bioavailability (BA) studies has gained inc...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yuan L,Huang C,Liu-Kreyche P,Voronin K,Fancher RM,Allentoff A,Zheng N,Iyer R,Zhu L,Pillutla R,Ji QC

更新日期：2019-02-20 00:00:00

abstract：:Croton cajucara Benth. is a medicinal plant popularly used in the Brazilian Amazonia, where it is known as sacaca, being consumed as tea, decoction or infusion of the leaves and stem bark. From a decoction of the leaves, a comprehensive phytochemical analysis was developed by liquid chromatography-mass spectrometry. M...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Nascimento AM,Maria-Ferreira D,Dal Lin FT,Kimura A,de Santana-Filho AP,Werner MFP,Iacomini M,Sassaki GL,Cipriani TR,de Souza LM

更新日期：2017-10-25 00:00:00

abstract：:The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with multi-way chemometrics is a robust methodology applicable for the analysis of raw materials, media,...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Calvet A,Li B,Ryder AG

更新日期：2012-12-01 00:00:00

abstract：:A rapid bioanalytical method was evaluated for the simultaneous determination of drug discovery compounds and their potential metabolites in plasma samples within 1 min run time by fast high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS). The fast HPLC-MS/MS system is achieved by using mini-co...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hsieh Y,Brisson JM,Wang G,Ng K,Korfmacher WA

更新日期：2003-09-19 00:00:00

abstract：:A rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry method was developed for simultaneous determination of eight constituents, uralsaponin C, uralsaponin F, 22β-acetoxyl-glycyrrhizin, 24-hydroxy-licorice-saponin E2, licorice-saponin G2, licorice-saponin E2, glycyrrhizin, and licorice...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Tao W,Duan J,Guo J,Li J,Tang Y,Liu P,Yang N

更新日期：2013-03-05 00:00:00

abstract：:A rapid and sensitive method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. Sample preparation involved liquid-liquid extraction with diethyl ether-dichloromethane (60:40, v/v). The analytes a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yan T,Li H,Deng L,Guo Y,Yu W,Fawcett JP,Zhang D,Cui Y,Gu J

更新日期：2008-12-01 00:00:00

abstract：:Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of alpha-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the pencil graphite electrode surface (PGE) at the 5' end amino group by using N-(dimeth...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kara P,Erdem A,Girousi S,Ozsoz M

更新日期：2005-06-01 00:00:00

abstract：:The objective of the present investigation was to separate, identify and characterize the major degradation products (DPs) of nelfinavir mesylate generated under hydrolytic, oxidative, photolytic and thermal stress conditions as advised in International Conference on Harmonization (ICH) guideline Q1A(R2). The drug was...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Tiwari RN,Bonde CG

更新日期：2011-06-01 00:00:00

abstract：:Analytical strategies for detecting drugs in biological samples rely on information on metabolism and elimination. 5F-PY-PICA belongs to the group of synthetic cannabinoids that are known to undergo excretion into the bile. The aims of this study were the in vitro identification of metabolites of 5F-PY-PICA and to det...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mardal M,Annaert P,Noble C,Oorts M,Linnet K

更新日期：2018-02-05 00:00:00

abstract：:Two UV spectrophotometric methods have been validated for the analysis of hydrolyzed tinidazole solutions. The pH of the samples must be 5.00-7.00 for both methods. The multiwavelength method may be used for samples degraded at pH 6-12 if the amount of conserved 5-nitroimidazole species is at least 93 mol.% of the ori...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Salo JP,Salomies H

更新日期：2003-03-10 00:00:00

abstract：:CDRI compound 81/470 is a new broad spectrum anthelmintic agent and is being developed for veterinary use. HPLC assay method for 81/470 in cow milk was developed and validated. The sample preparation consisted of protein precipitation, followed by extraction with ether. Separation was achieved on a C18 column using ac...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Nagaraja NV,Singh SK,Gupta RC

更新日期：1997-05-01 00:00:00

abstract：:Mixed-mode chromatography (MMC) is a fast growing area in recent years, thanks to the new generation of mixed-mode stationary phases and better understanding of multimode interactions. MMC has superior applications in the separation of compounds that are not retained or not well resolved by typical reversed-phase LC m...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang K,Liu X

更新日期：2016-09-05 00:00:00

abstract：:One of the most challenging aims in modern analytical chemistry and pharmaceutical analysis is to create models for drugs' behavior based on simulation experiments. Since drugs' effects are closely related to their molecular properties, numerous characteristics of drugs are used in order to acquire a model of passive ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kouskoura MG,Kachrimanis KG,Markopoulou CK

更新日期：2014-11-01 00:00:00

abstract：:A method for the separation of polymerized impurities in meropenem has been developed by gel filtration chromatography (GFC). The chromatographic conditions included a Superdex peptide HR 10/30 column and the mobile phase consisting of 0.01 mol L(-1) sodium phosphate buffer (pH 7.0), at a flow rate of 0.8 mL min(-1). ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cai SY,Hu CQ

更新日期：2005-03-09 00:00:00

abstract：:Concentrated solutions of doxycycline are often added to drinking water of animals for oral antibiotic therapy. However, stability concerns of doxycycline in solution involve an accurate selection of the solvent system to ensure that the active substance will remain within the acceptance range during the product shelf...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Jutglar M,Foradada M,Caballero F,Hoogmartens J,Adams E

更新日期：2018-09-10 00:00:00