Abstract:
:Demonstration of clonality is supportive of a diagnosis of malignancy in cases of lymphoproliferative disorders. Determination of clonality at the molecular level is currently accomplished by Southern analysis; however, the polymerase chain reaction offers a potential alternative that is rapid, simple, and less expensive. To test its feasibility as a diagnostic test, we amplified the DNA from 121 suspected lymphoproliferative disorders submitted for gene rearrangement studies. In comparison to Southern analyses, a sensitivity of 70% and specificity of 96% were obtained. To test the effect of primer variability in the joining region of the heavy-chain gene, we substituted a more degenerate primer but found no changes in sensitivity or specificity. We conclude that the polymerase chain reaction has current application with minute or fixed specimens and may generally serve as a rapid, initial evaluation for B-cell clonality, followed by Southern analysis in negative cases. However, higher overall sensitivity must be achieved before this technique can replace Southern analysis as the method of choice in determining clonal gene rearrangements.
journal_name
South Med Jjournal_title
Southern medical journalauthors
Mihalov ML,Huber S,Rachwalski E,Price JS,Hosso JM,Dizikes GJdoi
10.1097/00007611-199601000-00006subject
Has Abstractpub_date
1996-01-01 00:00:00pages
39-45issue
1eissn
0038-4348issn
1541-8243journal_volume
89pub_type
杂志文章abstract::We performed 12 arthrodeses of the knee and eight of the ankle, using the Hoffmann device as a compressor-fixator. For the knees, the double rectangular frame was used with the added fixation of a cylinder cast in six cases. In four cases a separate half frame was used in the anterior plane. Posttraumatic arthritis wa...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198311000-00018
更新日期:1983-11-01 00:00:00
abstract::A patient had a tracheoesophageal fistula resulting from malignant histiocytoma. Surgical treatment included esophagectomy and repair of the left main-stem bronchus. Gastrointestinal continuity was restored by a right colon interposition after chemotherapy. ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198409000-00044
更新日期:1984-09-01 00:00:00
abstract::Preeclampsia has been frequently misdiagnosed as an entity unrelated to pregnancy, with resultant delay in appropriate management. In the case presented here, severe preeclampsia complicated by hemolysis, elevated liver enzymes, and low platelet count ("HELLP syndrome") in a 32-week twin gestation was misdiagnosed as ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-199411000-00026
更新日期:1994-11-01 00:00:00
abstract::To determine sensitivity and specificity of magnetic resonance imaging (MRI) and ultrasonography (US) in the detection of breast implant rupture, and also to determine the relative merits of clinical examination and mammography, we studied 81 patients (160 implants). All patients had implants removed, thus allowing co...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-199604000-00009
更新日期:1996-04-01 00:00:00
abstract:BACKGROUND:Mortality rates for hyperthermia and hypothermia are higher in Alabama than nationally, and Alabama has twice as many hypothermia deaths as hyperthermia deaths. These causes of death have not been studied in the southeastern United States. We describe the epidemiology of heat- and cold-related deaths in Alab...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::We have described a patient with community-acquired, methicillin-resistant Staphylococcus epidermidis endocarditis that occurred on a prolapsing mitral valve. The patient was successfully treated with vancomycin and rifampin for six weeks and had no signs of relapse four months later. ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::For over a decade there has been concern about hepatitis related to halothane anesthesia. No dose relationship or other direct cause has ever been established, and jaundice has been found to occur after other anesthetics for surgical operations. Enflurane is a newer halogenated compound with a remarkable record of saf...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197807000-00012
更新日期:1978-07-01 00:00:00
abstract::Depression is among the most common of clinical conditions. Like problems such as anemia, it is not one disease, but a constellation of signs and symptoms which may occur as the result of several psychopatho-physiologic processes. This paper summarizes the recent dramatic advances in research into the neurochemistry o...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197502000-00015
更新日期:1975-02-01 00:00:00
abstract::A case of myocarditis, hepatic necrosis, and acute anuria associated with acute tonsillitis was described. The previously reported relation between myocarditis and tonsillitis not of diphtheritic or beta-hemolytic streptococcal origin was discussed, as well as the implications for management. ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197708000-00033
更新日期:1977-08-01 00:00:00
abstract::This is a case which highlights unique diagnostic challenges in the evaluation of a previously healthy patient presenting with a myelopathy initially most concerning for malignancy. However, timely recognition and nonoperative therapy of unexpected spinal sarcoidosis with corticosteroidal therapy was crucial in averti...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/SMJ.0b013e3181e90745
更新日期:2010-09-01 00:00:00
abstract::Ovarian vein thrombophlebitis is often misdiagnosed because of vague symptoms leading to unnecessary surgery, morbidity, and mortality. CT findings in such a case are very useful in making a definite diagnosis of ovarian vein thrombophlebitis. We consider CT the preferred method for evaluating pain in the right lower ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198911000-00022
更新日期:1989-11-01 00:00:00
abstract:OBJECTIVES:The objective of this population-based study was to examine the relationship between race, socioeconomic characteristics (socioeconomic status, SES), physician density, and colon cancer incidence in Alabama. METHODS:Data for 5,788 colon cancer cases from 1996 to 1999 provided by the Alabama Statewide Cancer...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/01.smj.0000184844.01148.10
更新日期:2005-11-01 00:00:00
abstract::We have reported a case of septicemia and meningitis due to alpha-hemolytic Streptococcus in a 5-day-old infant. The microbiologic and clinical characteristics of neonatal infection with this organism lead us to conclude that this bacterium can be pathogenic in the newborn and should not be dismissed as a contaminant....
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198303000-00032
更新日期:1983-03-01 00:00:00
abstract::Disseminated histoplasmosis is an unusual complication in endemic areas and has been reported in patients with an immunodeficient state, either from human immunodeficiency virus (HIV) infection or transplantation. In the non-HIV population, rare cases of disseminated histoplasmosis have been reported in patients with ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-199812000-00012
更新日期:1998-12-01 00:00:00
abstract::Treatment of major abdominal vascular injuries is a challenge. Since 1973, 93 patients have been treated at our institution for 147 abdominal vascular injuries. The majority of these injuries (67%) resulted from penetrating trauma in young men between 21 and 30 years of age. Upon admission 72% of the patients were in ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198510000-00003
更新日期:1985-10-01 00:00:00
abstract::We report the case of a 55-year-old woman who was thought to have systemic lupus erythematosus (SLE) for more than 13 years before Crohn's colitis was correctly diagnosed. The differentiation of Crohn's disease from SLE may be difficult, as illustrated by our case. Gastrointestinal symptoms, laboratory studies, and ra...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-199510000-00018
更新日期:1995-10-01 00:00:00
abstract:OBJECTIVES:To determine why there has been a decrease in the amount of applicants to Florida-based obstetrics and gynecology (OB/GYN) residency programs, and how this has been affected by the current medical liability climate. METHODS:Fourth year Florida medical students were surveyed about their concerns of a career ...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/01.smj.0000286757.82926.48
更新日期:2007-11-01 00:00:00
abstract::Reviewed are 200 cases in which endolaryngeal suspension microsurgical procedures were done using intravenous anesthesia and Venturi insufflation oxygenation. Patients included 177 adults and 23 children, ranging in age from 20 months to 88 years. No significant complications were observed. The technic allowed the glo...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197607000-00003
更新日期:1976-07-01 00:00:00
abstract::The use of tourniquets for upper and lower extremity operative procedures is controversial in patients with sickle cell hemoglobinopathies. The authors used Ovid and MEDLINE®, PubMed, and CINAHL® to detect English-language reports of any surgical procedure where a tourniquet was used on a patient with sickle cell dise...
journal_title:Southern medical journal
pub_type: 杂志文章,评审
doi:10.1097/SMJ.0b013e3181efaf3b
更新日期:2010-11-01 00:00:00
abstract::Preventive screening measures for low back pain are limited. Despite considerable evidence that anatomic narrowing of the lumbar spinal canal is a risk factor for specific back problems, difficulty in measuring the canal without unacceptable radiation exposure, cost, or discomfort has limited the assessment of its cli...
journal_title:Southern medical journal
pub_type: 杂志文章,评审
doi:10.1097/00007611-198908000-00012
更新日期:1989-08-01 00:00:00
abstract:OBJECTIVES:To assess the relation between renal function and delirium and to assess and compare the relation between cerebral white matter lesion (WML) and renal function as estimated by three formulas for the estimated glomerular filtration rate (eGFR) in older adult hospitalized veterans with and without delirium. M...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.14423/SMJ.0000000000001132
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:The primary aim of this study was to determine reasons why health care personnel (HCP) in a public health department chose or refused free influenza vaccinations offered at the worksite. METHODS:In an internal review board-approved study, we offered, through a health nurse at the site, self-administered sur...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/SMJ.0b013e3181eda3d5
更新日期:2010-10-01 00:00:00
abstract::Atopic dermatitis (AD) is a common diagnosis seen in both children and adults, and it is often the first manifestation of atopic disease. Research has shown a strong correlation between serum IgE levels, the severity of atopic dermatitis, and co-existing asthma and/or allergic rhinitis. Omalizumab (Xolair, East Hanove...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/SMJ.0b013e3181de0cf6
更新日期:2010-06-01 00:00:00
abstract::A prospective study of 45 granulocyte transfusions in children using continuous flow centrifugation is reported. During 13 episodes of proven or presumed infection, only two children failed to show a favorable response to granulocyte transfusion. The neutropenic child shows a significantly increased absolute granulocy...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197703000-00017
更新日期:1977-03-01 00:00:00
abstract::Between 1975 and 1978, over 200 knee arthroscopies were performed at the Emory University Hospitals; the findings were totally correct in 91%. During the same period, arthrograms were performed on 79 different patients, 39 of whom had confirming arthrotomies. The arthrograms were totally correct in 38%. Fifteen totall...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198111000-00009
更新日期:1981-11-01 00:00:00
abstract::Most patients with heart failure today are treated by primary care practitioners, not cardiologists. The Consensus Recommendations for the Management of Chronic Heart Failure, developed by the Advisory Council to Improve Outcomes Nationwide in Heart Failure, provide practice guidelines for the prevention, diagnosis, a...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::Between the years 1963 to 1982, 84 consecutive cases of intracranial complications of ear disease in pediatric patients were diagnosed and treated at the North Carolina Baptist Hospital. This group consisted of 65 patients with otitic meningitis, four with otitic brain abscess, four with otitic hydrocephalus, three wi...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-198504000-00018
更新日期:1985-04-01 00:00:00
abstract::An 18-year-old girl with multiple endocrine neoplasia type 2 (Sipple's syndrome) had a large intraventricular mass that proved to be a thrombus. She had no apparent precipitating factor for intracavitary thrombus formation. This is the first reported case of multiple endocrine neoplasia associated with ventricular thr...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::The antibody-coated bacteria (ACB) test was used to localize the site of urinary tract infection in a group of pediatric and adult patients. Duration of treatment of ACB-positive (upper tract) and ACB-negative (lower tract) infections was correlated with rates of recurrence during follow-up for six or more months. Two...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-197711000-00006
更新日期:1977-11-01 00:00:00
abstract::Recommendations concerning postoperative extubation after thymectomy for myasthenia gravis are presently based upon retrospective chart reviews. We present the results of a prospective investigation of time to extubation after thymectomy for 14 patients over a 12-month period based upon a protocol that included preope...
journal_title:Southern medical journal
pub_type: 杂志文章
doi:10.1097/00007611-199106000-00007
更新日期:1991-06-01 00:00:00