Abstract:
:Human intestinal mucosa consists of highly active epithelial cells in continual renewal and differentiation processes located at different portions of the villi. The crypt contains abundant replicating cells which, upon reaching the villus tip, acquire their fully differentiated state. Besides its well recognized role in bone cell homeostasis, calcitriol has been attributed a role in cellular differentiation and proliferation in normal leukocytes and myeloid leukemia cells. We have previously documented the presence and the distribution of specific calcitriol receptors in the cells of the small and large intestine from 13-20-wk-old human fetuses and that calcitriol was able to promote human intestinal epithelium proliferation or differentiation, in organ culture, depending upon fetal age. We now show that, whereas transcripts for calcitriol receptors are abundant from duodenum to colon, those for the 9-kD calcium-binding protein are present mainly in the duodenum and the jejunum and to a lesser extent in the ileum and the colon. Transcripts for 25-hydroxycholecalciferol-24-hydroxylase could not be detected in any of the intestine segments despite a prolonged exposition of the gels. Immunofluorescence staining for the 9-kD calcium-binding protein was exclusively observed in the epithelial cells of the small intestine and colon, the subepithelial layers being always negative. The 9-kD calcium-binding protein distribution along the crypt-villus axis appeared as a gradient, increasing from the developing crypt to the tip of the villus in the duodenum, jejunum, and ileum. Based on the present observations and on the fact that calcitriol promotes human fetal proliferation and differentiation, the presence of transcripts for calcitriol receptors and 9-kD calcium-binding protein in the intestinal cell opens interesting possibilities as of their role in the in utero human gut development and the control of colorectal cancers.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Delvin EE,Lopez V,Levy E,Ménard Ddoi
10.1203/00006450-199611000-00004subject
Has Abstractpub_date
1996-11-01 00:00:00pages
664-70issue
5eissn
0031-3998issn
1530-0447journal_volume
40pub_type
杂志文章abstract::We assessed auditory event-related potentials in small-for-gestational-age (SGA; 850 +/- 258 g, 28.9 +/- 3.3 gestational wk; n = 15) and appropriate for gestational age (AGA; 1014 +/- 231 g, 26.9 +/- 1.9 gestational wk; n = 20) preterm infants and healthy term infants (n = 22). An oddball paradigm was used with a harm...
journal_title:Pediatric research
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abstract::Heart rate turbulence (HRT) is a chronotropic response of the sinus heart rhythm to ventricular premature beat (VPB). Children show decreasing heart rate together with the maturation of the autonomic nervous system. The aim of the research was to assess the relationship between HRT parameters, age, and heart rate and ...
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journal_title:Pediatric research
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journal_title:Pediatric research
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doi:10.1203/01.PDR.0000072783.22373.FF
更新日期:2003-08-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/PDR.0b013e31822a36db
更新日期:2011-10-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/00006450-197703000-00009
更新日期:1977-03-01 00:00:00
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doi:10.1203/00006450-199412000-00006
更新日期:1994-12-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/00006450-197503000-00008
更新日期:1975-03-01 00:00:00
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更新日期:2005-07-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198803000-00019
更新日期:1988-03-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章,评审
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更新日期:2006-04-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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doi:10.1038/s41390-019-0502-9
更新日期:2020-01-01 00:00:00
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journal_title:Pediatric research
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doi:10.1038/s41390-019-0518-1
更新日期:2019-11-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/00006450-198111000-00009
更新日期:1981-11-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/00006450-199201000-00009
更新日期:1992-01-01 00:00:00
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journal_title:Pediatric research
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更新日期:1979-06-01 00:00:00
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更新日期:2007-08-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
abstract::Staphylococcus aureus has been isolated at an increasing rate from infants' stools during the last decades, but it is not known whether this species can colonize and persist in the intestinal microflora. To investigate this, 49 Swedish infants were followed prospectively from birth until 12 months of age. S. aureus wa...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200012000-00007
更新日期:2000-12-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
abstract::To investigate the difference in ventilatory response to exercise between children and young adults, we administered a treadmill progressive exercise test to seven boys (aged 8 to 11 y [group A]) and six male young adults (aged 14 to 21 y [group B]), who had a history of Kawasaki disease without significant coronary a...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199903000-00017
更新日期:1999-03-01 00:00:00
abstract::Tracheal epithelial cells obtained from adult and infant ferrets were grown in primary culture in vitro. Cells from adult animals grew readily, and many ciliated cells were observed in the cultures. Successful cultures were derived from infant animals, but cell number in infant and adult cultures began to decrease aft...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199107000-00016
更新日期:1991-07-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/PDR.0b013e3180686cc5
更新日期:2007-07-01 00:00:00
abstract::The field of regenerative medicine continues to make substantial advancements in therapeutic strategies addressing urologic diseases. Tissue engineering borrows principles from the fields of cell biology, materials science, transplantation and engineering in an effort to repair or replace damaged tissues. This review ...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1203/PDR.0b013e31816a704a
更新日期:2008-05-01 00:00:00
abstract::Functional disturbances in microcirculation in juvenile type 1 diabetes (T1D) are believed to underlie, in part, the later occurrence of cardiovascular complications. Some epidemiologic studies suggested greater risk of microvascular complications in those with T1D-risk genotypes of human leukocyte antigen (HLA). We i...
journal_title:Pediatric research
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doi:10.1203/PDR.0b013e318165bfd4
更新日期:2008-04-01 00:00:00