Abstract:
INTRODUCTION:A greater supply of tendinous tissue can be obtained through tissue engineering technology with increasing application of adult stem cells. It is well known that adipose-derived stem cells (ADSCs), found in abundance in adipose tissue, have the same differentiating capacity as mesenchymal stem cells yet have the advantage of being easily isolated. In the present study, we combined the great facility of ADSCs to differentiate with the application of an external mechanical stimulus to successfully create an in vitro reconstructed tendonlike structure with a microcapillary network. MATERIALS AND METHODS:Hyalonect meshes (Fidia Advanced Biopolymers, Abano Terme, Padova, Italy) were used as scaffold. Human ADSCs were seeded onto the biomaterials, and the cell/scaffold constructs were cultured under mechanical stress for up to 15 days. Human tenocytes were used in the same conditions as control. Performance was assessed by histology, immunochemistry, ultrastructure, and biomolecular analysis. RESULTS:Adipose-derived stem cells seeded onto Hyalonect adhered and differentiated along the entire surface of the biomaterial and began to infiltrate within its structure. Subsequently, endothelial cells migrated, forming a capillary in the new extracellular matrix. CONCLUSIONS:This technique allowed for the creation of a vascularized tendon equivalent that could easily be detached from the bioreactor, thus facilitating its implant at the lesion site. These results highlight the biologic performance of biodegradable hyaluronic acid-based (HYAFF-11) scaffolds, which were shown to be suitable for deposition of the autologous extracellular matrix critical for ADSCs differentiation.
journal_name
Ann Plast Surgjournal_title
Annals of plastic surgeryauthors
Vindigni V,Tonello C,Lancerotto L,Abatangelo G,Cortivo R,Zavan B,Bassetto Fdoi
10.1097/SAP.0b013e3182583e99subject
Has Abstractpub_date
2013-12-01 00:00:00pages
664-70issue
6eissn
0148-7043issn
1536-3708journal_volume
71pub_type
杂志文章abstract::We report the use of topic negative pressure for seromas resulting from mobilization of tensor fascia lata miocutaneous flap in the trochanteric areas for pressure sores. In 5 consecutive patients we successfully treated seroma with the use of external and internal topic subatmospheric pressure. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31819adf94
更新日期:2009-12-01 00:00:00
abstract::Endoscopic-assisted surgery allows remote incision placement and provides an illuminated, magnified operative field. We have applied these principles to perform neurolysis of the zygomaticotemporal nerve and removal of a fixation wire under endoscopic control in a patient with pain and tenderness at the site of a prev...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199608000-00012
更新日期:1996-08-01 00:00:00
abstract::Patients with homozygous sickle cell disease pose an enormous challenge to the reconstructive surgeon. Historically, the risk of attempting flap reconstruction was considered prohibitive. A successful case of immediate breast reconstruction with a "supercharged" bipedicled transverse rectus abdominis musculocutaneous ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199511000-00011
更新日期:1995-11-01 00:00:00
abstract::The tensor fascia lata myocutaneous flap provides a reliable autogenous building block for anatomical lower abdominal wall reconstruction. Preservation of innervation allows maintenance of voluntary motor control and protective sensation. The excellent blood supply of this flap is particularly helpful in reconstructin...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198108000-00014
更新日期:1981-08-01 00:00:00
abstract::Using an enzyme-linked immunosorbent assay, we investigated the amount of cell surface fibronectin and the amount released to the culture medium in human skin fibroblasts during successive cultivation. Cell surface fibronectin did not show a remarkable change in quantity through early and midpassage, but it decreased ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198503000-00010
更新日期:1985-03-01 00:00:00
abstract::Resection of malignant soft-tissue tumors of the face often results in defects of skin, lining, and contour. When local tissues are unavailable, the folded/multiple skin island forearm free flap has been used to correct complex lining, skin, and contour defects concomitantly. This study is a retrospective review of al...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200112000-00006
更新日期:2001-12-01 00:00:00
abstract::Cytokines are peptide mediators of inflammation that are being extensively studied in several aspects of disease. I provide an introduction to the general features of cytokine biology. Cytokines may be considered as either proinflammatory or anti-inflammatory compounds, and they also have potent hematopoietic effects....
journal_title:Annals of plastic surgery
pub_type: 杂志文章,评审
doi:10.1097/00000637-199511000-00021
更新日期:1995-11-01 00:00:00
abstract::Periosteal chondroma has not been previously described as a cause of carpal tunnel syndrome. This report describes a case of periosteal chondroma developing in the floor of the carpal canal and presenting as carpal tunnel syndrome. ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198403000-00010
更新日期:1984-03-01 00:00:00
abstract::Regional metastases from head and neck cutaneous tumors are uncommon, and most present within 2 years from initial diagnosis. Occasionally such metastases may manifest at a later date, increasing the possibility of being derived from a second noncutaneous primary cancer of the head and neck region. The authors studied...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199903000-00010
更新日期:1999-03-01 00:00:00
abstract::In the literature, few studies based on clinical and histological evaluation analyze skin structural changes after transplantation to the oral cavity. Ten patients affected by squamous cell carcinoma of the oral cavity who were reconstructed with a free forearm flap were included in the current study to analyze skin a...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-200210000-00005
更新日期:2002-10-01 00:00:00
abstract::Hematogenous ostemyelitis is infrequently seen in adults and primary involvement of the hand skeleton is extremely rare. Little has been reported about the foci of hematogenously spread infections. Two cases of hematogenous osteomyelitis of the hand originating from dental maxillary infections were reported. The first...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-199607000-00016
更新日期:1996-07-01 00:00:00
abstract::Lipedema is a condition characterized by abnormal deposition of adipose tissue in the lower extremities leading to circumferential bilateral lower extremity enlargement typically seen extending from the hips to the ankles. Diagnosis of the condition is often challenging, and patients frequently undergo a variety of un...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e318215791e
更新日期:2012-03-01 00:00:00
abstract::Sodium warfarin is one of the most commonly used oral anticoagulants. It is not without complications. A case is presented of a 56-year-old woman on well-regulated sodium warfarin therapy who suffered unexplained necrosis and gangrene of the breast. She underwent total mastectomy for debridement because of the extensi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198508000-00012
更新日期:1985-08-01 00:00:00
abstract::Incisional hernias develop in 2% to 11% of patients who undergo laparotomy. Prosthetic mesh repair provides more strength, tension-free closure, and decreased recurrence rates as compared to primary tissue repairs. Complications-fistula formation, adhesions, skin erosion, and seroma/abscess formation-however, include ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e318212f380
更新日期:2012-02-01 00:00:00
abstract::Avulsion fractures can pose technical difficulties for surgical fixation. The fragments are often small and around finger joints with access being difficult due to attachment of ligaments or tendons. Traditionally, these have been treated using K wires, lag screws, or pullout sutures.Hook plates were originally descri...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000001119
更新日期:2017-09-01 00:00:00
abstract::The almost single disadvantage of polyurethane film dressings, an uncontrolled leakage, is probably as often described as its numerous advantages for split-thickness skin graft donor sites. We solved this problem by perforating the polyurethane film, which permits a controlled leakage into a secondary absorbent dressi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31818790b4
更新日期:2009-08-01 00:00:00
abstract::Swallowing and communication are occasionally impaired after free jejunal transfer. Here, the relationship between surgical procedure and functional outcome was analyzed in 236 patients undergoing free jejunal transfer after total laryngopharyngectomy from 1992 through 2003. Swallowing and communication functions were...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31817439c5
更新日期:2009-01-01 00:00:00
abstract::We present a case of a nail defect, which was reconstructed by free composite nail combined with the palmar pocket method without vascular anastomoses. A 40-year-old man lost his nail of the right middle finger by trauma 1 year ago. A total nail composite graft, composed of germinal and sterile matrices, and proximal ...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/01.sap.0000164731.99795.fe
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:The volar advancement flap of the thumb described by Moberg has been used for pulp defects up to 1 to 1.5 cm since its mobility is quite limited due to the stiff nature and fibrous connections of the regional skin with underlying structures. There have been several attempts to increase the mobility of this u...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e318216e6b1
更新日期:2012-02-01 00:00:00
abstract::This paper describes a new method of creating a natural anterior hairline using microsurgical free scalp flaps. This operative method is a one-stage procedure and the new hairline has normal hair density and natural hair direction. The donor flap is raised mainly from the parietooccipital portion of the scalp. The met...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198007000-00004
更新日期:1980-07-01 00:00:00
abstract:PURPOSE:Rhabdomyolysis often occurs after traumatic compartment syndrome, and high morbidity and mortality have been reported with the acute kidney injury that develops subsequently. We focused on the risk factors for rhabdomyolysis and acute kidney injury in patients with traumatic compartment syndrome. We also analyz...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000460
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:The use of internal mammary perforators (IMPs) as recipient vessels in autologous free flap breast reconstruction has many additional benefits compared with the internal mammary or thoracodorsal vessels. Our goals were to analyze the characteristics of these vessels and to evaluate the reliability of using t...
journal_title:Annals of plastic surgery
pub_type: 临床试验,杂志文章
doi:10.1097/SAP.0b013e318270704b
更新日期:2014-08-01 00:00:00
abstract::Prosthetic graft material is often used for the repair of abdominal wall defects that result from trauma, infection, neoplastic, or congenital deformities. A new material, porcine small intestinal submucosa, has been successfully used as an arterial and venous graft material in both canine and primate animal models wi...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::The medial canthus is supported by several structures with a complicated 3-dimensional arrangement in a narrow space. Although the medial canthal tendon occupies a major portion of the area, the medial canthal support structures include the following entities: Horner's muscle, the medial rectus capsulopalpebral fascia...
journal_title:Annals of plastic surgery
pub_type: 杂志文章,评审
doi:10.1097/SAP.0b013e3182a6365c
更新日期:2015-04-01 00:00:00
abstract::The previously reported surgical methods for eyelid entropion are basically manipulations of the skin or the conjunctival surface. When entropion is marked, manipulations also involve the tarsus. In the most widely used method, the eyelashes are directed outward by horizontal wedge resection of an appropriate amount o...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/01.sap.0000106426.86478.dc
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Complications after immediate breast reconstruction pose a significant challenge to the reconstructive surgeon. Known risk factors include smoking, obesity, age, and adjuvant oncologic therapies. Less is known about the association between axillary lymph node dissection (ALND) and the development of postoper...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000653
更新日期:2016-11-01 00:00:00
abstract::We report 2 cases of patients with thumb polydactyly that were difficult to classify into either Wassel type VI with a remarkable hypoplastic metacarpal or Wassel type VII (triphalangeal thumb). The radial digit was functional but hypoplastic, and the ulnar digit presented a dominant appearance but no active motion. T...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0b013e31821b6ced
更新日期:2013-01-01 00:00:00
abstract::A more direct measurement of implant capsule contracture is needed for the laboratory study of this phenomenon. Most of the previous methods for quantitation of contracture rely on measurements of deformability. Because contraction of an implant capsule is in fact a decrease in the surface area of the capsule, a metho...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/00000637-198311000-00008
更新日期:1983-11-01 00:00:00
abstract:ABSTRACT:Subungual exostosis is a benign tumor that occurs under the nail plate of the distal phalanx and causes nail deformity and pain. There are many reports of recurrence and postoperative nail deformity, and the surgical approach needs to be reassessed. Two typical surgical approaches are from the nail fold and th...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000002619
更新日期:2020-12-15 00:00:00
abstract::Severe motorcycle spoke injuries of the heel lead to Achilles tendon defects, calcaneal tubercle exposure or loss, and overlying soft tissue defects, which are challenging to treat. Given the special physiological and developmental characteristics of children, severe spoke injuries of the heel in children are especial...
journal_title:Annals of plastic surgery
pub_type: 杂志文章
doi:10.1097/SAP.0000000000000820
更新日期:2016-12-01 00:00:00