Abstract：
:In Mizoram, the origin and molecular nature of Anopheles species is poorly understood, despite the region having high malarial incidence and rich biodiversity. A diagnostic PCR assay for distinguishing the Cellia subgenera members of Anopheles species was developed based on the interspecific ITS2 variation. No intraspecific variation was found and the size (362-604bp) and GC content (48.8-58.9%) of the ITS2 were highly variable among Anophelines. The ITS2 of A. vagus is significantly longer than those of other Anopheles species. Significant relationship was observed among repeats, minimum free energy and RNA secondary structures. Different types of microsatellites were identified and among them dinucleotide, pentanucleotide and polynucleotide microsatellites were predominant. Variation in the length of the ITS2 between species was due to indels in simple repeats. Four domain types of RNA secondary structures were identified and the lowest free energy values were predicted using the computer software, RNAfold. Types I and II were observed only in Neocellia and Myzomyia series and Types III and IV were common in Neocellia and Pyretophorus series. ITS2-based PCR protocol provides a means for vector ecologists, malaria epidemiologists and control personnel to accurately identify members of the subgenera Cellia and a better understanding of their genomic status in Mizoram.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Zomuanpuii R,Ringngheti L,Brindha S,Gurusubramanian G,Senthil Kumar Ndoi
10.1016/j.actatropica.2012.12.001subject
Has Abstractpub_date
2013-03-01 00:00:00pages
309-19issue
3eissn
0001-706Xissn
1873-6254pii
S0001-706X(12)00379-8journal_volume
125pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:Anopheles balabacensis balabacensis and Anopheles minimus are the main malaria vectors in Thailand. In a cleared forested foothill area in the central part of the country A. minimus was the most prevalent anopheline species found, only 6 specimens of A. b. balabacensis being collected over a 3-year period. Cattle were...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:Extracellular vesicles (EVs) play a key role in host-parasite interactions. Previous studies have shown that parasites can release microRNA (miRNA) containing EVs, which can transfer their contents to host cells and regulate gene expression in recipient cells. However, a little is known about the secretion of EVs by t...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105718
更新日期：2020-12-01 00:00:00
abstract：:Five years after a single dose treatment, prevalence, intensity and morbidity of schistosomiasis mansoni were evaluated in Agua Branca, a low endemic community in the South East Brazil (cure rate 94%). At community level, prevalence showed a decrease from 38.9 to 24.5% and the intensity of infection dropped from 119.5...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(01)00190-5
更新日期：2002-02-01 00:00:00
abstract：:Definitions of the term 'challenge' as applied to the African trypanosomiases are reviewed. Data from one West and one East African site show simple linear relationships between the incidence of trypanosomiasis in both humans and animals, and either the amount of man-tsetse contact, or the Apparent Density of flies. D...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：
更新日期：1985-03-01 00:00:00
abstract：:The aims of this study were to describe the occurrence of dengue in space and time and to assess the relationships between dengue incidence and entomologic indicators. We selected the dengue autochthonous cases that occurred between September 2005 and August 2007 in São José do Rio Preto to calculate incidence rates b...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.11.017
更新日期：2015-02-01 00:00:00
abstract：:Four strains of Trypanosoma cruzi (Y, BG, M and Peru) retain their ability to infect Rhodnius prolixus and to produce virulent infections in mice for from 12 to 39 years. About 60 or more metacyclic trypomastigotes were consistently lethal to mice. The mean number of metacyclics per bug ranged from 1.2 to 17.3 x 10(3)...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Aedes aegypti and Aedes albopictus were reported to be significant as vectors of dengue fever. In Taiwan, the latter is distributed throughout the island while the former appears only south of the Tropic of Cancer; i.e., 23.5°N. In the past decade, there were five outbreaks with over 1000 cases of dengue fever in Taiw...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2013.10.013
更新日期：2014-02-01 00:00:00
abstract：:Dengue and Saint Louis encephalitis virus are among the most important emerging viruses transmitted by mosquitoes at the global scale, and from 2009 onward both diseases have reached temperate Argentina. To test whether the urbanization level can be used as a predictor for the infestation levels of container-breeding ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.09.015
更新日期：2013-12-01 00:00:00
abstract：:The midguts of female mosquitoes, Culex tarsalis, were examined electron microscopically during the digestion of a meal from either artificial sources (i.e., 100% serum or defibrinated rabbit blood) or vertebrate hosts. Intense intercellular staining was apparent when the meal was derived from the vertebrate host or d...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:Cattle were exposed to natural trypanosome challenge in an area of high Glossina density (Samandéni, Burkina Faso) for various periods of time during 1982, 1983, 1984 and 1985. All of 30 Zebu proved to be sensitive to trypanosomiasis i.e. they died or were treated in extremis in 10 +/- 4 weeks. Twenty-one (31%) Baoulé...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after contr...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2004.04.003
更新日期：2004-09-01 00:00:00
abstract：:A vaccine to protect sheep, goats, and bovines against hydatid disease caused by the cysts of Echinococcus granulosus is prepared as a recombinant fusion protein expressed in Escherichia coli. Solubilised inclusion bodies are injected, together with Quil A, subcutaneously on two occasions 1 month or more apart, and in...
journal_title：Acta tropica
pub_type： 指南,杂志文章,实务指引,评审
doi：10.1016/s0001-706x(02)00219-x
更新日期：2003-02-01 00:00:00
abstract：:Conventional anthelmintics such as albendazole could not achieve complete cure of trichinellosis till now. The antimalarial mefloquine mediates oxidative stress and disrupts lysosomal functions leading to cell death. Therefore, the aim of this work was to investigate the effect of mefloquine on experimental acute and ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105760
更新日期：2021-01-01 00:00:00
abstract：:The information in this protocol covers from the basic steps and material necessary to start a sand fly colony up to the specific details which are important to the success of a Nyssomyia neivai colony. The greatest problems in our colony of Ny. neivai were solved with specific care, for instance, using vermiculite an...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.07.010
更新日期：2017-10-01 00:00:00
abstract：:A recently described species of black fly, Simulium wayani Takaoka and Chen, from the island of Timor was chromosomally mapped to provide insights into its evolutionary and biogeographic history. The morphologically based species status of S. wayani is supported by a suite of fixed chromosomal rearrangements and uniqu...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.02.017
更新日期：2019-05-01 00:00:00
abstract：:Loop-mediated isothermal amplification (LAMP) is a novel molecular method that accelerates and facilitates DNA amplification and detection under isothermal conditions. It represents a revolution in molecular biology by reducing the high cost, turnaround time and technicality of polymerase chain reaction and other ampl...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2012.02.004
更新日期：2012-06-01 00:00:00
abstract：:Several studies have reported on similar in vitro cellular responses to different malaria-antigen preparations in both malaria-primed and un-primed donors. Whether intact live parasites can exert a distinct type of response in either of the two groups is not well known. In this study, we developed a simple three-step ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2005.08.007
更新日期：2006-01-01 00:00:00
abstract：:A new Myxobolus species is described infecting gill filaments of the endangered ornamental fish Otocinclus cocama from Peruvian Amazon. In a total of 35 fish examined, five (14.3%) had myxozoan plasmodia. Taxonomic analysis was performed integrating multiple characters, including morphometrical, biological traits, ssr...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105545
更新日期：2020-10-01 00:00:00
abstract：:Transmission risk for vector borne disease is greatest during periods of heightened vector activity. Triatomine bugs (Hemiptera: Reduviidae), which transmit Trypanosoma cruzi, the causative agent of Chagas disease, are generally considered nocturnal, but their patterns of activity in controlled settings have rarely be...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.06.012
更新日期：2018-09-01 00:00:00
abstract：:In vitro cultured Plasmodium falciparum gametocytes were fed to Anopheles gambiae (G3) mosquitoes to identify parasite population characteristics useful for predicting successful mosquito infections. Parameters were collected from an initial study of 90 infections over a two year period and a second study of 55 infect...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/0001-706x(94)90117-1
更新日期：1994-10-01 00:00:00
abstract：:Tropomyosins of invertebrates are pan-allergens responsible for wide spread allergic reactions against seafood and arthropods. As invertebrate tropomyosins are highly conserved, helminth tropomyosins are likely to show properties similar to these medically important allergens. Studies with a monoclonal antibody, NR1, ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.05.010
更新日期：2010-10-01 00:00:00
abstract：:The involvement of the central nervous system (CNS) by Schistosoma mansoni may or may not cause clinical manifestations. When symptomatic, neuroschistosomiasis mansoni (NSM) is one of the most severe presentations of this infection. The neurological manifestations are due to numerous granulomas grouped in confined are...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2008.04.007
更新日期：2008-11-01 00:00:00
abstract：:Vector control and effective case management are currently the backbone strategies of malaria control. Kitgum district, an area of perennial holoendemic malaria transmission intensity in Northern Uganda, appears to have experienced a malaria epidemic in 2015. This study aimed to describe the malaria trends in Kitgum G...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.08.023
更新日期：2018-12-01 00:00:00
abstract：:This study was conducted from 2008 to 2013 to determine the animal health status of Ivory Coast and neighboring countries (Burkina Faso, Ghana, Togo and Benin) for African swine fever (ASF) and classical swine fever (CSF), and to assess the risk factors for ASF introduction in Ivory Coast. Ivory Coast had probably bee...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.10.027
更新日期：2017-02-01 00:00:00
abstract：:Chronic malaria severely affects the immune system and causes polyclonal B-cell activation, as evidenced by the presence of hypergammaglobulinemia, elevated levels of autoantibodies, loss of B-cell memory and the frequent occurrence of Burkitt's lymphomas (BL) in children living in malaria endemic areas. Previous stud...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.05.005
更新日期：2011-08-01 00:00:00
abstract：:The reptile-associated Borrelia represent a monophyletic group of bacteria transmitted by several species of hard ticks, which has been reported to only infect amphibians and reptiles in Eurasia and Middle East, however, this bacterial group has not been studied in North America. The aim of this study was to assess th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105422
更新日期：2020-05-01 00:00:00
abstract：INTRODUCTION:This article reports, for the first time, the occurrence of Panstrongylus megistus in the Brazilian Western Amazon. METHODS:Specimens of P. megistus were collected in the cities of Rio Branco, Acre and Extrema, Rondônia. RESULTS:The number of triatomine species in the State of Acre increased from eight t...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.02.032
更新日期：2018-06-01 00:00:00
abstract：:The coadministration of tubercidin and ethidium to mice infected with Trypanosoma gambiense gave a better parasite clearance than either of the single drugs. The combination was also more toxic to the mice but the inclusion of nitrobenzylthioinosinate in the therapy significantly alleviated the toxicity of the drug co...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:Controlling dengue fever in Australia and internationally, relies heavily upon the actions of residents as well as community education and awareness of the risks. Although it has been well established in medical anthropology that the success of health interventions is highly dependent upon a clear grasp of lay knowled...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.05.012
更新日期：2010-10-01 00:00:00
abstract：:During the last decade Entamoeba moshkovskii has become relevant given its capacity to infect humans, especially when considering that it is morphologically indistinguishable from E. histolytica. For a long time, E. moshkovskii was considered as a free living amoeba, but in the last decade it has been demonstrated tha...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2012.05.012
更新日期：2012-09-01 00:00:00
