Molecular detection of reptile-associated Borrelia in Boa constrictor (Squamata: Boidae) from Veracruz, Mexico.

Abstract:

:The reptile-associated Borrelia represent a monophyletic group of bacteria transmitted by several species of hard ticks, which has been reported to only infect amphibians and reptiles in Eurasia and Middle East, however, this bacterial group has not been studied in North America. The aim of this study was to assess the presence of Borrelia spirochetes in blood samples of native reptiles of Mexico. Blood samples were directly obtained from individuals, DNA extractions were performed using Chelex-100. The Borrelia detection was performed by conventional PCR. From 102 reptiles tested, only five individuals of Boa constrictor were positive for the presence of DNA of the reptile-associated Borrelia group. Supported by phylogenetic analysis, this study presents the first record of these spirochetes group in Mexico, and initial evidence of B. constrictor as a host of this group.

journal_name

Acta Trop

journal_title

Acta tropica

authors

Morales-Diaz J,Colunga-Salas P,Romero-Salas D,Sánchez-Montes S,Estrada-Souza IM,Ochoa-Ochoa LM,Becker I,Flores-Primo A,Cruz-Romero A

doi

10.1016/j.actatropica.2020.105422

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

105422

eissn

0001-706X

issn

1873-6254

pii

S0001-706X(19)31732-2

journal_volume

205

pub_type

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