当前位置： SCI文献检索 > CHEMICO-BIOLOGICAL INTERACTIONS期刊下所有文献 > Analysis and characterization of eight estradiol inducible genes and a strong promoter from the steroid degrading marine bacterial strain S19-1.

Analysis and characterization of eight estradiol inducible genes and a strong promoter from the steroid degrading marine bacterial strain S19-1.

Abstract：

:Buttiauxella strain S19-1 is a new marine bacterium, isolated from the Baltic Sea, which can degrade steroids. In this report, a meta-genomic approach was used to isolate estradiol inducible genes from S19-1. SalI-fragments from the chromosomal DNA of S19-1 were ligated into plasmid pKEGFP2 bearing an EGFP gene as the reporter system. All resulting plasmids harboring SalI-fragments were transformed into Escherichia coli HB101 to measure the relative fluorescent units (RFU). E. coli cells showing higher RFU after estradiol induction than those without estradiol induction, were selected and the respective plasmids were sequenced. Sequences of 8 positive plasmids were analyzed and aligned by BLAST. Among the predicted genes we found similarities to the major facilitator superfamily, glycerol dehydratase activator, formate acetyltransferase activating enzyme, histidinol-phosphate/aromatic aminotransferase, ABC-transporter, transcriptional regulator nadR, lipoate-protein ligase A, and alcohol phosphatidyl-transferase. Interestingly, one of the E. coli cell clones (containing plasmid p302) showed up in green color by normal light microscopy, which indicated that a strong promoter was present in this plasmid. Sequencing and deletion-mutagenesis revealed that the putative promoter comprises a 108 bp DNA fragment within p302, from which the putative -10 and -35 regions are TTTGAT and TTGGTT, respectively. The promoter might be used to construct S19-1 mutants in which steroid degradation occurs at high levels.

journal_name

Chem Biol Interact

journal_title

Chemico-biological interactions

authors

Zhang T,Xiong G,Maser E

doi

10.1016/j.cbi.2012.11.018

subject

Has Abstract

pub_date

2013-02-25 00:00:00

pages

159-67

issue

1-3

eissn

0009-2797

issn

1872-7786

pii

S0009-2797(12)00262-1

journal_volume

202

pub_type

杂志文章

相关文献

CHEMICO-BIOLOGICAL INTERACTIONS文献大全
  • Therapeutic efficacy of green tea polyphenols on cellular thiols in 4-Nitroquinoline 1-oxide-induced oral carcinogenesis.

    abstract：:In cancer, a high flux of oxidants not only depletes the cellular thiols, but damages the whole cell as well. Epidemiological studies suggest green tea may mitigate cancers in human and animal models for which several mechanisms have been proposed. In the present investigation, the levels of cellular thiols such as re...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2004.06.006

    authors： Srinivasan P,Sabitha KE,Shyamaladevi CS

    更新日期：2004-10-15 00:00:00

  • Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers.

    abstract：:An important application of primary hepatocyte cultures is for hepatotoxicity research. In this paper, gel entrapment culture of rat hepatocytes in miniaturized BAL system were evaluated as a potential in vitro model for hepatotoxicity studies in comparison to monolayer cultures. After exposure for 24 and 48 h to acet...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2006.05.005

    authors： Shen C,Zhang G,Qiu H,Meng Q

    更新日期：2006-07-25 00:00:00

  • Target cells for methylsulphonyl-2,6-dichlorobenzene in the olfactory mucosa in mice.

    abstract：:Previously we reported that methylsulphonyl-2,6-dichlorobenzene, 2, 6-(diCl-MeSO(2)-B), was irreversibly bound to the olfactory mucosa of mice and induced necrosis of the Bowman's glands with subsequent neuroepithelial degeneration and detachment. In this study, autoradiography and histopathology were used to determin...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(00)00187-3

    authors： Bahrami F,van Hezik C,Bergman A,Brandt I

    更新日期：2000-10-16 00:00:00

  • Purification of transgenic plant-derived recombinant human acetylcholinesterase-R.

    abstract：:Nicotiana benthamiana plants were engineered to express a codon-optimized gene encoding the human acetylcholinesterase-R (AChE) isoform. The transgenic plants expressed the protein at >0.4% of total soluble protein, and the plant-produced enzyme was purified to homogeneity. Following lysis, procainamide affinity chrom...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.10.097

    authors： Geyer BC,Muralidharan M,Cherni I,Doran J,Fletcher SP,Evron T,Soreq H,Mor TS

    更新日期：2005-12-15 00:00:00

  • Aryl hydrocarbon hydroxylase in mouse mammary gland: in vitro study using mammary cell lines.

    abstract：:The effects of 3-methylcholanthrene (MCA), 5,6-benzoflavone (betaNF), 7,8-benzoflavone (alphaNF) and pregnenolone 16alpha-carbonitrile (PCN) upon aryl hydrocarbon hydroxylase (AHH) were determined in primary mammary gland epithelial cell cultures prepared from the C3Hf-/Ki mouse. MCA elevated AHH activity by 3--4 fold...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(77)90067-9

    authors： Chuang AH,Howard EF,Bresnick E

    更新日期：1977-04-01 00:00:00

  • Polyproline-rich peptides associated with Torpedo californica acetylcholinesterase tetramers.

    abstract：:Acetylcholinesterase (AChE) terminates cholinergic neurotransmission by hydrolyzing acetylcholine. The collagen-tailed AChE tetramer is a product of 2 genes, ACHE and ColQ. The AChE tetramer consists of 4 identical AChE subunits and one polyproline-rich peptide, whose function is to hold the 4 AChE subunits together. ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2020.109007

    authors： Toker L,Silman I,Zeev-Ben-Mordehai T,Sussman JL,Schopfer LM,Lockridge O

    更新日期：2020-03-01 00:00:00

  • DNA modification induced by 6-mercaptopurine riboside in murine embryos.

    abstract：:The cytostatic drug 6-mercaptopurine riboside (6-MPr) was investigated in mice in order to test the hypothesis that the teratogenicity of this antimetabolite is paralleled by an incorporation into the DNA of the embryos during organogenesis. DNA modification in the embryos was analysed 4 h following s.c. administratio...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(94)90086-8

    authors： Platzek T,Schwabe R,Rahm U,Bochert G

    更新日期：1994-10-01 00:00:00

  • Caspase-dependent and -independent mechanisms in apoptosis induced by hydroquinone and catechol metabolites of remoxipride in HL-60 cells.

    abstract：:The hydroquinone and catechol like metabolites, NCQ344 and NCQ436 respectively, of the antipsychotic remoxipride have recently been demonstrated to induce apoptosis in myeloperoxidase (MPO)-rich human bone marrow progenitor and HL-60 cells [S.M. McGuinness, R. Johansson, J. Lundstrom, D. Ross, Induction of apoptosis b...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(00)00188-5

    authors： Inayat-Hussain SH,McGuinness SM,Johansson R,Lundstrom J,Ross D

    更新日期：2000-08-15 00:00:00

  • The metabolism and antitumour activity of the enantiomers of cis- and trans-4-methylcyclophosphamide.

    abstract：:4-Methylcyclophosphamide, an analogue of the antitumour agent cyclophosphamide, exists in cis and trans forms, each of which comprises a pair of optical isomers. The extents of metabolism by rat liver microsomes during 20 min were compared for the four steroisomers incubated separately, and for the racemic cis and tra...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(77)90140-5

    authors： Farmer PB,Jarman M,Facchinetti T,Pankiewicz K,Stec WJ

    更新日期：1977-07-01 00:00:00

  • Drug bioactivation and protein adduct formation in the pathogenesis of drug-induced toxicity.

    abstract：:Adverse drug reactions (ADRs) remain a major complication of drug therapy and can be classified as 'on-target' or 'off-target' (idiosyncratic) reactions. On-target reactions can be predicted from the known primary or secondary pharmacology of the drug and often represent an exaggeration of the pharmacological effect o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2010.09.011

    authors： Park BK,Laverty H,Srivastava A,Antoine DJ,Naisbitt D,Williams DP

    更新日期：2011-06-30 00:00:00

  • Mechanism of unbalanced growth-induced cell damage. I. A probable role for hydrolytic enzymes synthesis.

    abstract：:This study examines the relationship between cell progress into the state of unbalanced growth, hydrolytic enzyme activities and cell survival during the exposure of L5178Y cells to hydroxyurea (HU), excess thymidine (dThR), hydroxyurea with excess of four deoxyribonucleosides (dNR) or excess dTHR with deoxycytidine (...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(86)90006-2

    authors： Malec J,Przybyszewski WM,Grabarczyk M,Sitarska E,Czartoryska B

    更新日期：1986-03-01 00:00:00

  • La(3+), Gd(3+) and Yb(3+) induced changes in mitochondrial structure, membrane permeability, cytochrome c release and intracellular ROS level.

    abstract：:Lanthanides (Ln) were known to induce cell apoptosis, which might be the results of their effects on mitochondria (MT). This study was trying to clarify the role of MT and reactive oxygen species (ROS) in Ln-induced apoptosis. We found that micromolar or lower concentration of La(3+), Gd(3+) and Yb(3+) bound to MT and...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(03)00072-3

    authors： Liu H,Yuan L,Yang X,Wang K

    更新日期：2003-07-25 00:00:00

  • Numb ameliorates necrosis and inflammation in acute kidney injury induced by cisplatin.

    abstract：:Cisplatin induces acute renal failure in humans and mice.Tubular apoptosis, necrosis and inflammation are the primary pathogenesis of cisplatin-induced acute kidney injury(AKI). We previously reported that the depletion of Numb from proximal tubules exacerbates tubular cells apoptosis in cisplatin-induced AKI, however...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2020.109251

    authors： Liu Z,Li Z,Chen Z,Li C,Lei L,Wu X,Li Y

    更新日期：2020-10-01 00:00:00

  • Single immunoglobulin IL-1 receptor-related protein attenuates the lipopolysaccharide-induced inflammatory response in A549 cells.

    abstract：:The lipopolysaccharide (LPS)-Toll-like receptor 4 (TLR4) signaling pathway in alveolar epithelial cells plays an important role in many pathologic processes such as acute lung injury (ALI). The single immunoglobulin IL-1 receptor-related protein (SIGIRR) is an inhibitor of LPS-TLR4 signaling, but its expression and fu...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2009.11.022

    authors： Feng T,Yunfeng N,Jinbo Z,Zhipei Z,Huizhong Z,Li L,Tao J,Yunjie W

    更新日期：2010-02-12 00:00:00

  • Protective effect of zinc against cadmium hepatotoxicity depends on this bioelement intake and level of cadmium exposure: a study in a rat model.

    abstract：:It was estimated, in a rat model of moderate and relatively high chronic human exposure to cadmium (Cd), whether enhanced zinc (Zn) consumption may prevent Cd-induced liver injury and if the possible protective effect of this bioelement depends on its intake. For this purpose, the structure and function of the liver o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2011.05.008

    authors： Rogalska J,Pilat-Marcinkiewicz B,Brzóska MM

    更新日期：2011-09-30 00:00:00

  • Connecting glutathione with immune responses to occupational methylene diphenyl diisocyanate exposure.

    abstract：:Methylene diphenyl diisocyanate (MDI) is among the leading chemical causes of occupational asthma world-wide, however, the mechanisms of disease pathogenesis remain unclear. This study tests the hypothesis that glutathione (GSH) reacts with MDI to form quasi-stable conjugates, capable of mediating the formation of MDI...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2013.06.005

    authors： Wisnewski AV,Liu J,Redlich CA

    更新日期：2013-09-05 00:00:00

  • Biochemical alterations elicited in rat liver microsomes by oxidation and reduction products of chloroform metabolism.

    abstract：:The feasibility of an oxygen-independent mechanism of chloroform bioactivation was indicated by the covalent binding to lipid and protein occurring in anaerobic incubations of CHCl3 and microsomes in the presence of NADPH. Under these conditions, the loss of cytochrome P-450 and the inhibition of related monoxygenases...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(86)80063-1

    authors： Testai E,Vittozzi L

    更新日期：1986-09-01 00:00:00

  • Combined treatment with vitamin K2 and PTH enhanced bone formation in ovariectomized rats and increased differentiation of osteoblast in vitro.

    abstract：:Osteoporosis is accompanied by insufficient osteogenic capacity. Several lines of evidence suggested that solutions to enhance osteoblastogenesis were important strategies for osteoporotic bone defect repair. This study investigated the effect of combined treatment with vitamin K2 and PTH on bone formation in calvaria...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2019.01.012

    authors： Weng SJ,Yan DY,Gu LJ,Chen L,Xie ZJ,Wu ZY,Tang JH,Shen ZJ,Li H,Bai BL,Boodhun V,Yang L

    更新日期：2019-02-25 00:00:00

  • Ex vivo anticholinesterase activity of benzoic acid derivatives.

    abstract：:p-Aminobenzoic acid (p-ABA) derivatives were evaluated as acetylcholinesterase inhibitors (AChEIs). Finding a correlation between AChE activity with the partition coefficient (log P) and the highest occupied molecular orbital (HOMO) energy of the compounds. ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.10.055

    authors： Correa-Basurto J,Espinosa-Raya J,Vázquez-Alcántara I,Flores-Sandoval CA,Trujillo-Ferrara J

    更新日期：2005-12-15 00:00:00

  • An albumin-butyrylcholinesterase for cocaine toxicity and addiction: catalytic and pharmacokinetic properties.

    abstract：:Butyrylcholinesterase (BChE, EC 3.1.1.8) is important in human cocaine metabolism despite its limited ability to hydrolyze this drug. Efforts to improve the catalytic efficiency of this enzyme have led to a quadruple mutant cocaine hydrolase, "CocH", that in animal models of addiction appears promising for treatment o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2008.04.024

    authors： Gao Y,LaFleur D,Shah R,Zhao Q,Singh M,Brimijoin S

    更新日期：2008-09-25 00:00:00

  • Artesunate induces autophagy dependent apoptosis through upregulating ROS and activating AMPK-mTOR-ULK1 axis in human bladder cancer cells.

    abstract：:Artesunate is a kind of derivative of artemisinin, which possesses potent anti-cancer effect in addition to its anti-malarial property. And autophagy was a highly conserved process, exerting a double-edged effect in cancer cell survival. Besides, apoptosis is a programmed cell death program, crucial to cell homeostasi...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2020.109273

    authors： Zhou X,Chen Y,Wang F,Wu H,Zhang Y,Liu J,Cai Y,Huang S,He N,Hu Z,Jin X

    更新日期：2020-11-01 00:00:00

  • The CADM2/Akt pathway is involved in the inhibitory effect of miR-21-5p downregulation on proliferation and apoptosis in esophageal squamous cell carcinoma cells.

    abstract：:Esophageal squamous cell carcinoma (ESCC), the main subtype of esophageal cancer, is the eighth most common cancer worldwide. Cell adhesion molecule 2 (CADM2) has been reported to be a tumor suppressor and is usually downregulated in several cancers. However, the role of CADM2 in ESCC remains unknown. The aim of the p...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2018.04.021

    authors： Li X,Chen D,Li M,Gao X,Shi G,Zhao H

    更新日期：2018-05-25 00:00:00

  • Combined effect of vanadium(V) and chromium(III) on lipid peroxidation in liver and kidney of rats.

    abstract：:Since chromium(III) was demonstrated to have antioxidative action, we have decided to study the effect of this element on V-induced LPO in liver and kidney of rats. Outbred 2-month-old, albino male Wistar rats received daily, for a period of 12 weeks: group I (control), deionized water to drink; group II, sodium metav...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.11.008

    authors： Scibior A,Zaporowska H,Ostrowski J,Banach A

    更新日期：2006-02-25 00:00:00

  • Binding of chemical carcinogens and mutagens to rat hemoglobin.

    abstract：:The alkylation of hemoglobin is a proposed dose monitor for chemical carcinogens and mutagens. The binding of fifteen chemical carcinogens and mutagens to rat hemoglobin was determined. Direct acting carcinogens and indirect acting carcinogens including aromatic amines, halogenated hydrocarbons, nitrosamines, polycycl...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(81)90048-x

    authors： Pereira MA,Chang LW

    更新日期：1981-01-01 00:00:00

  • Lon protease and eiF2α are involved in acute, but not prolonged, antiretroviral induced stress response in HepG2 cells.

    abstract：:Lon protease, an ATP dependent mitochondrial protease, is important in mitochondrial protein maintenance. Disruption of protein homeostasis and mitochondrial dysfunction is associated with lipodystrophy, metabolic syndrome and accelerated aging, and are commonly observed in patients on long term antiretroviral therapy...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.03.021

    authors： Nagiah S,Phulukdaree A,Chuturgoon AA

    更新日期：2016-05-25 00:00:00

  • Regulatory crosstalk and interference between the xenobiotic and hypoxia sensing pathways at the AhR-ARNT-HIF1α signaling node.

    abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates many of the responses to toxic environmental chemicals such as TCDD or dioxin-like PCBs. To regulate gene expression, the AhR requires its binding partner, the aryl hydrocarbon receptor nuclear translocator (ARNT). ARNT is als...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章,评审

    doi：10.1016/j.cbi.2014.05.001

    authors： Vorrink SU,Domann FE

    更新日期：2014-07-25 00:00:00

  • Screening of O-ethyl O-4-nitrophenyl phosphoramidate (ENPP) for delayed neuropathic potential.

    abstract：:O-Ethyl-O-4-nitrophenylphosphoramidate is a short-acting anticholinesterase and a possible candidate for a prophylactic agent against nerve agents since human acetylcholinesterase inhibited by this agent undergoes rapid spontaneous reactivation which can be accelerated further, if necessary, by treatment with oximes. ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(93)90072-7

    authors： Johnson MK,Read DJ

    更新日期：1993-06-01 00:00:00

  • Sesquiterpene lactones of Moquiniastrum polymorphum subsp. floccosum have antineoplastic effects in Walker-256 tumor-bearing rats.

    abstract：BACKGROUND AND AIM:This study aimed to evaluate the in vivo antitumor actions and toxicity of the dichloromethane fraction (F1B) of Moquiniastrum polymorphum subsp. floccosum (formerly Gochnatia polymorpha ssp. floccosa), composed of sesquiterpene lactones, against Walker-256 carcinosarcoma in rats. METHODS:Male Wista...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2015.01.018

    authors： Martins GG,Lívero FA,Stolf AM,Kopruszinski CM,Cardoso CC,Beltrame OC,Queiroz-Telles JE,Strapasson RL,Stefanello MÉ,Oude-Elferink R,Acco A

    更新日期：2015-02-25 00:00:00

  • The effects of indole-3-acetic acid on human and horse serum butyrylcholinesterase.

    abstract：:Butyrylcholinesterase (BChE) constitutes the first line defense in the serum of higher organisms and is a marker for toxic exposure. Indole-3-acetic acid (IAA) is a major plant growth hormone of the auxin class, affecting cell enlargement, and differentiation. As a result of the industrial usage, this agrochemical is ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.10.061

    authors： Bodur E,Cokugras AN

    更新日期：2005-12-15 00:00:00

  • A synthetic chalcone derivative, 2-hydroxy-3',5,5'-trimethoxychalcone (DK-139), triggers reactive oxygen species-induced apoptosis independently of p53 in A549 lung cancer cells.

    abstract：:2-Hydroxy-3',5,5'-trimethoxychalcone (named DK-139) is a synthetic chalcone derivative that has anti-inflammatory, anti-tumor, and endoplasmic reticulum-mediated apoptosis activities. However, the mode of action of DK-139 on reactive oxygen species (ROS)-induced apoptosis remains unknown. In this study, we found that ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2018.11.003

    authors： Gil HN,Jung E,Koh D,Lim Y,Lee YH,Shin SY

    更新日期：2019-01-25 00:00:00