Abstract：
:In erectile dysfunction (ED) research, monocultures of cavernous endothelial cells (CECs) and smooth muscle cells (CSMCs) have been reported, but a CEC-CSMC coculture system is still lacking. In the present study, we wished to investigate the feasibility of setting up such a system and test whether it can be used for diabetic ED research. Cavernous tissues were obtained from patients undergoing surgery for penile prosthesis. CSMCs were isolated by explant culture and verified by calponin staining. CECs were isolated by binding to CD31 antibody, followed by magnetic capture. These CECs were nearly 100% pure endothelial cells as determined by flow cytometric analysis for endothelial markers CD31, vWF and eNOS. Functional analyses, that is, low-density lipoprotein (LDL) uptake and capillary tube formation, also confirmed their endothelial phenotype. When cocultured with CSMCs, CECs formed capillary-like structures, and based on the extent of this capillary-like network, it was determined that a ratio of 1:4 in cell number between CECs and CSMCs was better than ratios of 1:1 and 1:9. It was also found that direct contact between CECs and CSMCs was necessary and a coculture period of 3 weeks was optimal. Autologous CSMCs were better than allogeneic CSMCs, and fibroblasts were completely incompetent. When treated with high glucose (25 mM), the CEC-CSMC coculture expressed significantly lower level of CD31 but significantly higher level of collagen-IV (Col-IV), and the diameter of the capillaries increased significantly, when compared with normal glucose (5 mM)-treated cocultures. These data are consistent with previously observed changes in the cavernous tissues of diabetic patients and thus suggest that the coculture system could be utilized for diabetic ED research.
journal_name
Int J Impot Resjournal_title
International journal of impotence researchauthors
Ning H,Lin G,Lue TF,Lin CSdoi
10.1038/ijir.2012.36subject
Has Abstractpub_date
2013-03-01 00:00:00pages
63-8issue
2eissn
0955-9930issn
1476-5489pii
ijir201236journal_volume
25pub_type
杂志文章abstract：:Sildenafil citrate, marketed as Viagra, for the treatment of erectile dysfunction, has a proven record of safety in humans as predicted by the results of extensive pharmacological and toxicological testing in animals and in vitro, and confirmed by pharmacokinetic exposure data. The aim of this paper is to review succi...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/sj.ijir.3901232
更新日期：2004-12-01 00:00:00
abstract：:Fifty partial and non-responders (Clinical Global Impression-Sexual Function (CGI-SF) score>2), out of 76 men who completed a 6-week, double-blind, placebo-controlled trial of sildenafil treatment for serotonergic antidepressant-associated sexual dysfunction, were eligible for an additional 6-week trial of open-label ...
journal_title：International journal of impotence research
pub_type： 杂志文章,随机对照试验
doi：10.1038/sj.ijir.3901502
更新日期：2007-03-01 00:00:00
abstract：:We describe a case of gunshot wound to the corpora cavernosa caused by a low-velocity bullet in a 43-year-old man. He volunteered that his lover's husband shot him with a handgun. The bullet had penetrated the right gluteal region with no exit wound causing a right corpus cavernosum lesion. A penile colour-duplex dopp...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901472
更新日期：2006-11-01 00:00:00
abstract：:The Hypogonadism In Males study estimated the prevalence of hypogonadism in men aged > or =45 years. A sub-analysis of patients not receiving testosterone (T) therapy was conducted. Blood draw times were 0800-1000 and 1000-1200 hours. Total T (TT) was not influenced by draw time for any age group; however, significant...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901580
更新日期：2008-03-01 00:00:00
abstract：:In order to assess the effectiveness of sildenafil under routine conditions of use in primary care settings and to evaluate its impact on patient's life satisfaction and partner's satisfaction with treatment for erectile dysfunction (ED), an open, multicentre, observational, prospective study was designed in which 281...
journal_title：International journal of impotence research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/sj.ijir.3900782
更新日期：2001-12-01 00:00:00
abstract：:Tunica albuginea (TA) of the penis, which has an important role in mechanism of erection, is composed mainly of collagen bundles. Both aging and diabetes mellitus (DM) were reported to be associated with many alterations in collagen content and architecture in several body tissues. So, the aim of this work was to eval...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3900389
更新日期：1999-04-01 00:00:00
abstract：:Unconsummated marriage (UM) is the failure to perform successful sexual intercourse at the beginning of the marriage. Vaginismus has been traditionally reported as the leading cause. ED is also a leading cause for UM. This appears to be a significant problem in the conservative middle-Eastern societies and in the deve...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/ijir.2013.24
更新日期：2014-01-01 00:00:00
abstract：:Nitric oxide (NO) is a physiologic signal essential to penile erection, and disorders that reduce NO synthesis or release in the erectile tissue are commonly associated with erectile dysfunction. NO synthase (NOS) catalyzes production of NO from L-arginine. While both constitutively expressed neuronal NOS (nNOS) and e...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/sj.ijir.3901209
更新日期：2004-06-01 00:00:00
abstract：:The isometric tension measurement and in vitro autoradiography were used in clitoral cavernosum smooth muscle (CSM). Angiotensin ANG III, ANG IV, ANG II and ANG I induced contractions in clitoral CSM strips. ANG III and ANG I- induced contraction was five times less active than ANG II, whereas ANG IV-induced contracti...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3900824
更新日期：2002-04-01 00:00:00
abstract：:The objective of the study was to evaluate the relationship between the sexual position and severity of penile fracture (PF). We studied 90 patients with PF. The mechanism of injury and the sexual position was assessed. We divided our sample by the etiology of the fracture in six groups: (a) masturbation or penile man...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/ijir.2017.24
更新日期：2017-09-01 00:00:00
abstract：:During penile pharmacotesting, anxiety may overcome complete smooth muscle relaxation and lead to false positive diagnosis of organic male erectile dysfunction (ED). We tested the efficacy and safety of re-dosing of PGE1 alone versus combination of PGE, and the alpha1,2-blocker phentolamine (PHE) to improve an incompl...
journal_title：International journal of impotence research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/sj.ijir.3900477
更新日期：2000-02-01 00:00:00
abstract：:Premature ejaculation (PE), the most common sexual dysfunctions in men, is characterized by loss or absence of ejaculatory control. PE can be classified as either a lifelong or acquired condition. Although the prevalence of lifelong PE is rather low in the general male population, recent studies demonstrated that the ...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/ijir.2012.12
更新日期：2012-09-01 00:00:00
abstract：:In extracavernosal vascular tissue, smooth muscle tone is modulated by a balance between angiotensin II (Ang II) and nitric oxide (NO). We hypothesized that these substances also play an important role in regulating cavernosal smooth muscle contractility. We therefore studied the in vitro effects of an Ang II receptor...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3900261
更新日期：1997-09-01 00:00:00
abstract：:Radical prostatectomy (RP) is a commonly performed procedure for the management of prostate cancer. While documented oncologic outcome for early stage disease is excellent, functional impairments such as incontinence and erectile dysfunction (ED) are common after the procedure. Recent evidence has implicated cavernous...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/ijir.2011.26
更新日期：2011-09-01 00:00:00
abstract：:Modulation of the adrenergic activity and interfering with channels such as potassium channels may affect relaxation and contraction of the corpus cavernosum. Sildenafil is a selective phosphodiesterase-5 inhibitor, proven effective in treating erectile dysfunction. In this study, the effect of sildenafil citrate on a...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901590
更新日期：2007-11-01 00:00:00
abstract：:Erectile dysfunction (ED) can be an early and first sign of an underlying systemic disease. A screening program is offered by the Israeli Defense Force for career servicemen at the staff periodic examination center (SPEC), the aim of which was early detection of morbidity. We introduced the Sexual Human Inventory for ...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901173
更新日期：2004-08-01 00:00:00
abstract：:Erectile dysfunction is a common condition that affects men over age 40. It is highly related to obesity. The corpus cavernosum is the most important structure involved in erection. The aim of this study was to evaluate the structure of the corpus cavernosum of mice fed with a high energy density diet (HED). At 3 mont...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/s41443-018-0089-4
更新日期：2019-03-01 00:00:00
abstract：:In a study on the diagnosis and treatment of erectile dysfunction (ED) in general practice, a self-selected sample of 90 general practitioners (GPs) (76 males, 14 females) from a predominantly urban population of 525 GPs were interviewed. It appeared that 59% would not actively interrogate a patient when an erection p...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Anxiety plays a major role in the development of the problems associated with erectile dysfunction (ED). Psychological and behavioural responses to ED can lead to a vicious cycle of increased uneasiness, distance and conflicts. This in turn leads to a lower frequency of sexual encounters, less time spent together and ...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/sj.ijir.3900994
更新日期：2003-04-01 00:00:00
abstract：:It is well known that the administration of phosphodiesterase type-5 inhibitors (PDE5-Is) may improve erectile function (EF) recovery after bilateral nerve-sparing radical prostatectomy (BNSRP). The aim of our study was to identify predictors of the use of a high number of PDE5-Is (one or more per week) after surgery ...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/ijir.2014.10
更新日期：2014-11-01 00:00:00
abstract：:In several studies we have recently demonstrated that orgasm induces prolactin secretion in healthy males and females. This suggests that prolactin may form a feedback regulator of the refractory period following orgasm. To examine this position we investigated the prolactin response of a healthy multi-orgasmic male s...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3900823
更新日期：2002-04-01 00:00:00
abstract：:Our interim analysis of a phase II clinical trial on low-intensity extracorporeal shockwave therapy (Li-ESWT) for men with erectile dysfunction (ED) has demonstrated it is safe with no reported adverse outcomes. Interim analysis has demonstrated Sexual Encounter Profile 3 (ability to maintain erection till completion)...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/s41443-019-0115-1
更新日期：2019-05-01 00:00:00
abstract：:We evaluated the prevalence of erectile dysfunction (ED) in a cross-sectional community-based random sample of Egyptian men. ED was correlated with the socioeconomic status, risk factors and quality of life. Married men in Ismailia province were interviewed at home. Data were processed for 805 men with mean age of 43....
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901000
更新日期：2003-08-01 00:00:00
abstract：:No oral medication has proved to be clearly beneficial for Peyronie's disease (PD). We investigated the safety and efficacy of coenzyme Q(10) (CoQ(10)) supplementation in patients with early chronic PD. We conducted a randomized clinical trial of 186 patients with chronic early PD. Patients were randomly assigned to e...
journal_title：International journal of impotence research
pub_type： 杂志文章,随机对照试验
doi：10.1038/ijir.2010.20
更新日期：2010-09-01 00:00:00
abstract：:Multiple system atrophy (MSA) is a progressive neurodegenerative disease characterized by parkinsonism and cerebellar, autonomic, urinary, and/or pyramidal dysfunction. Urinary and erectile dysfunction (ED) symptoms are prominent early features in men with MSA. Autonomic failure, considered until recently to be the ca...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901014
更新日期：2003-08-01 00:00:00
abstract：:Erectile dysfunction (ED), generally associated with reduced sexual desire and sometimes with orgasmic or ejaculatory dysfunction, is the major revealing symptom of hyperprolactinemia (HPRL) in men, a condition that should not be neglected since many cases result from pituitary tumors, likely to result in serious comp...
journal_title：International journal of impotence research
pub_type： 杂志文章,评审
doi：10.1038/sj.ijir.3901043
更新日期：2003-10-01 00:00:00
abstract：:The objective of this study was to investigate the predictive validity of the Dutch translation of the Golombok Rust Inventory of Sexual Satisfaction (GRISS) for the presence of clinically relevant sexual dysfunctions and patient's need of help. A total of 57 male urological outpatients (age 50.7+/-13.2 y; range 27-77...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3900966
更新日期：2003-04-01 00:00:00
abstract：:In an observational study in men with erectile dysfunction (ED) consulting a general practitioner (GP) or urologist in Belgium, demographics, ED characteristics (including erection hardness score), co-morbidities and treatment expectations were evaluated using a structured questionnaire. In total, 341 GPs and 41 urolo...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/ijir.2008.7
更新日期：2008-07-01 00:00:00
abstract：:The association between different antihypertensive drugs and erectile dysfunction (ED) was examined in a cohort of type II diabetes patients identified in the UK General Practice Research Database (GPRD). The GPRD contains details of diagnoses, prescribing, investigations, risk factors, outcomes, and hospital referral...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：10.1038/sj.ijir.3901041
更新日期：2003-10-01 00:00:00
abstract：:We evaluated the effects of chronic penile denervation on cavernous tissue morphology and function in 36 Sprague-Dawley rats. At age seven weeks, 18 animals underwent bilateral cavernous nerve neurectomy: 18 animals underwent sham operation as a control. A functional, biochemical and morphological assessment of the ra...
journal_title：International journal of impotence research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
