Abstract：
AIMS:The regulation of vascular diameter by vasoconstrictors and vasodilators requires that vascular smooth muscle cells (VSMCs) be physically coupled to extracellular matrix (ECM) and neighbouring cells in order for a vessel to mechanically function and transfer force. The hypothesis was tested that integrin-mediated adhesion to the ECM is dynamically up-regulated in VSMCs during contractile activation in response to a vasoconstrictor and likewise down-regulated during relaxation in response to a vasodilator. METHODS AND RESULTS:VSMCs were isolated from the Sprague-Dawley rat cremaster muscles. Atomic force microscopy (AFM) with fibronectin (FN)-functionalized probes was employed to investigate the biomechanical responses and adhesion of VSMCs. Responses to angiotensin II (Ang II; 10(-6) M) and adenosine (Ado; 10(-4) M) were recorded by measurements of cell cortical elasticity and cell adhesion. The results showed that Ang II caused an immediate increase in adhesion (+27%) between the probe and cell. Cell stiffness increased (+70%) in parallel with the adhesion change. Ado decreased adhesion (-15%) to FN and reduced (-30%) stiffness. CONCLUSION:Changes in the receptor-mediated activation of the contractile apparatus cause parallel alterations in cell adhesion and cell cortical elasticity. These studies support the hypothesis that the regulation of cell adhesion is coordinated with contraction and demonstrate the dynamic nature of cell adhesion to the ECM. It is proposed that coordination of adhesion and VSMC contraction is an important mechanism that allows for an efficient transfer of force between the contractile apparatus of the cell and the extracellular environment.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Hong Z,Sun Z,Li Z,Mesquitta WT,Trzeciakowski JP,Meininger GAdoi
10.1093/cvr/cvs239subject
Has Abstractpub_date
2012-10-01 00:00:00pages
73-80issue
1eissn
0008-6363issn
1755-3245pii
cvs239journal_volume
96pub_type
杂志文章abstract：OBJECTIVE:The aim was to examine the role of nitric oxide (NO) in the myocardial microcirculation. METHODS:Open chest anaesthetised dogs received intracoronary infusion of NG-monomethyl-L-arginine (L-NMMA; 0.5 mg.kg-1 over 30 min). Myocardial microvascular extraction fraction of a small hydrophilic solute (technetium ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1995-06-01 00:00:00
abstract：:Primary cardiomyopathy is an important cause of mortality in children and adults. Apart from inherited disorders of myocardial contractile and structural proteins, several defects of energy metabolism may cause cardiomyopathy. Most of the energy required for myocardial contraction is derived from aerobic metabolism. F...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(97)00141-7
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVE:Ischaemic preconditioning may be mediated by oxygen free radicals generated during preconditioning. Conflicting results have been reported regarding the effect of superoxide dismutase (SOD) in attenuating the cardioprotective effect of preconditioning. The aim of the study was to reconcile this conflict by ex...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.7.980
更新日期：1994-07-01 00:00:00
abstract：OBJECTIVE:The mechanism of spontaneous myogenic tone was investigated in isolated arteriolar segments. METHODS:Arterioles were isolated from rat cremaster muscle. Segments were endothelium-denuded and mounted in a pressure myograph at 75 mmHg. Under this condition, segments spontaneously constricted from a passive dia...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(98)00161-8
更新日期：1999-01-01 00:00:00
abstract：:An isolated working rat heart preparation was used to characterise the temperature-dependency of the anti-ischaemic properties of nifedipine. In this study hearts were subjected to pre-ischaemic infusion with the St Thomas' cardioplegic solution with or without added nifedipine (0.075 mumol X litre-1). Hearts were the...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/19.2.95
更新日期：1985-02-01 00:00:00
abstract：OBJECTIVES:Matrix metalloproteinases (MMPs) are plausible candidates for prediction of unstable coronary syndromes. We hypothesised that the MMP-3 polymorphism (- 1171, 5A/6A) would relate to coronary plaque characteristics and unstable clinical presentation. METHODS AND RESULTS:Forty patients with de novo presentatio...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2007.05.003
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:Determine if ischemic tolerance is reduced in the setting of experimental heart failure (HF). METHODS:Dogs were paced for 3 weeks at 240 BPM to induce heart failure which was confirmed with hemodynamic and echocardiographic measurements. The pacemaker was turned off 30 min prior to the ischemia study. Normal...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/0008-6363(96)00028-4
更新日期：1996-08-01 00:00:00
abstract：:Oxygen-derived free radicals are thought to contribute to the initiation and progression of cardiovascular disease via several different mechanisms, such as consumption of nitric oxide, oxidation of proteins and lipids, and activation of redox-sensitive signalling cascades. Vascular NADPH oxidases are important source...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2004.08.007
更新日期：2005-01-01 00:00:00
abstract：AIMS:Tungstate reduces blood pressure in experimental animal models of both hypertension and metabolic syndrome, although the underlying mechanisms are not fully understood. Given that the large-conductance voltage- and Ca(2+)-dependent K(+) (BK) channel is a key element in the control of arterial tone, our aim was to ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs139
更新日期：2012-07-01 00:00:00
abstract：AIMS:Alterations in cardiac acid-base balance can produce profound impact on excitation-contraction coupling and precipitate cardiac dysfunction and arrhythmias. A member of the solute carrier (SLC) family, Slc26a6, has been shown to be a chloride-hydroxyl exchanger and the predominant chloride-bicarbonate exchanger in...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvt195
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that mild hypothermia potentiates the cardioprotection afforded by ischaemic preconditioning so that infarct size limitation can be obtained after coronary artery occlusion (CAO) durations which exceed the cardioprotective range (> 90 min) of either hypothermia or ischaemic precondition...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(97)00222-8
更新日期：1998-01-01 00:00:00
abstract：OBJECTIVES:Collagen synthesis is one of the major mechanisms of primary atherosclerotic plaque growth and is likely to be similarly important in restenosis. The patterns of collagen gene expression in human restenosis and associations with thrombosis/hemorrhage have not been described. METHODS:Using human coronary art...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：AIMS:Ivabradine (Iva) has shown beneficial structural and functional effects in clinical and experimental heart failure (HF), but its action in combination with mechanical unloading (MU), such as during treatment with left ventricular assist devices (LVAD), is unknown. The aim of this study was to investigate the effec...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs318
更新日期：2013-02-01 00:00:00
abstract：AIMS:Proliferation of mammalian cardiomyocytes stops during the first weeks after birth, preventing the heart from regenerating after injury. Recently, several studies have indicated that induction of cardiomyocyte proliferation can be utilized to regenerate the mammalian heart. Thus, it is important to identify novel ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp360
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to determine whether chronic beta-adrenergic receptor (beta-AR) stimulation induces proliferation of human cardiac fibroblasts and to investigate the mechanism(s) involved. METHODS AND RESULTS:In vitro cultures of human cardiac fibroblasts were established from biopsies of right atr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00729-0
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:Air bubbles entering the coronary artery may have harmful effects on cardiac function. From the physical point of view it is the relatively high surface tension of the blood-air interface which causes bubbles to trap in small vessels. The aim of the present study was to reduce depression of myocardial functio...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(97)00063-1
更新日期：1997-06-01 00:00:00
abstract：OBJECTIVE:Persistent atrial fibrillation (AF) results in an impairment of atrial function. In order to elucidate the mechanism behind this phenomenon, we investigated the gene expression of proteins influencing calcium handling. METHODS:Right atrial appendages were obtained from eight patients with paroxysmal AF, ten ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(99)00045-0
更新日期：1999-05-01 00:00:00
abstract：:The effects on an acute myocardial infarction of equipotent hypotensive doses of nitroglycerin and dipyridamole were studied using the thermographic technique in open-chest dogs. Nitroglycerin did not reduce the cool ischaemic area in any of the nine dogs studied. In six of the dogs, in which the ischaemic region coul...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.5.281
更新日期：1979-05-01 00:00:00
abstract：AIMS:The heart contains c-kit(+) progenitors that maintain cardiac homeostasis. Cardiac c-kit(+) cells are multipotent and give rise to myocardial, endothelial and smooth muscle cells, both in vitro and in vivo. C-kit(+) cells have been thoroughly investigated for their stem cell activity, susceptibility to stress cond...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvq292
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:The highly selective adenosine A1 receptor agonist, 2-chloro-N6-cyclopentyl-adenosine (CCPA), has been shown to be as cardioprotective as ischaemic preconditioning when evaluated with an early staining method using tetrazolium. However, tetrazolium-positive tissue measured 3 h after reperfusion may still over...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.4.652
更新日期：1993-04-01 00:00:00
abstract：AIMS:Treatment with the anticancer drug taxol (TXL), which polymerizes the cytoskeleton protein tubulin, may evoke cardiac arrhythmias based on reduced human cardiac sodium channel (Na(v)1.5) function. Therefore, we investigated whether enhanced tubulin polymerization by TXL affects Na(v)1.5 function and expression and...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvp352
更新日期：2010-03-01 00:00:00
abstract：:Blood vessels are permanently exposed to stretch and shear stress due to blood pressure and blood flow. Significant variations in the mechanical environment, of physiological or pathophysiological nature, occur in vivo. These trigger acute changes in vessel diameter that tend to restore basal levels of tensile and she...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2006.05.008
更新日期：2006-07-15 00:00:00
abstract：:Acadesine is the prototype of a new class of therapeutic compounds termed adenosine regulating agents. Acadesine is pharmacologically silent in normal circumstances, but its activity becomes operative during situations of net ATP catabolism, when it increases local adenosine levels. Thus acadesine is able to harness t...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/27.1.43
更新日期：1993-01-01 00:00:00
abstract：OBJECTIVE:The aim was to test the hypothesis that in normal humans angiotensin II would stimulate local release of noradrenaline under basal conditions or during a sympathetic stimulus provided by lower body negative pressure (LBNP). METHODS:Nine healthy volunteers received intra-arterial infusions of angiotensin II, ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.5.663
更新日期：1994-05-01 00:00:00
abstract：OBJECTIVE:The aim was to examine ventricular brain natriuretic peptide (B-type natriuretic peptide, BNP) gene expression and to determine its relationship with ventricular BNP and circulating BNP levels in paediatric cardiac transplant recipients, over a three year period after transplantation. METHODS:Total RNA extra...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/27.12.2135
更新日期：1993-12-01 00:00:00
abstract：AIMS:Our recent work demonstrated that common cardiovascular progenitor cells are characterized and induced by the expression of the transcription factor mesoderm posterior1 (MesP1) in vertebrate embryos and murine embryonic stem cells. As the proliferative potential of stem cell-derived cardiomyocytes is limited, it i...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvr158
更新日期：2011-10-01 00:00:00
abstract：:Although the existence of t-tubules in mammalian cardiac ventricular myocytes has been recognized for a long time, it now appears that their structure and function are more complex than previously believed. Recent work has provided evidence that many of the key proteins underlying excitation-contraction coupling are l...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvm002
更新日期：2008-01-15 00:00:00
abstract：OBJECTIVE:Insulin protects cardiomyocytes from apoptosis. Insulin resistance usually refers to a defect in the ability of insulin to stimulate glucose uptake. It is unknown, however, whether or not insulin resistance compromises the cell-protective effect of the hormone. Caspases are a family of cysteine proteases that...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2007.07.012
更新日期：2007-12-01 00:00:00
abstract：:Isolated, buffer perfused guinea pig hearts were used to study the effects of sotalol on arrhythmias and electrophysiology during 30 min of myocardial ischaemia, induced by reducing coronary flow to 10% of control, and subsequent reperfusion. Action potentials were recorded using the floating microelectrode technique ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/18.7.397
更新日期：1984-07-01 00:00:00
abstract：AIMS:Mast cells participate importantly in abdominal aortic aneurysms (AAAs) by releasing inflammatory cytokines to promote vascular cell protease expression and arterial wall remodelling. Mast cells accumulate in AAA lesions during disease progression, but the exact chemokines by which mast cells migrate to the site o...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs262
更新日期：2012-12-01 00:00:00
