Abstract:
:Mitochondria are the essential eukaryotic organelles that produce most cellular energy. The energy production and supply by mitochondria appear closely associated with the continuous shape change of mitochondria mediated by fission and fusion, as evidenced not only by the hereditary diseases caused by mutations in fission/fusion genes but also by aberrant mitochondrial morphologies associated with numerous pathologic insults. However, how morphological change of mitochondria is linked to their energy-producing activity is poorly understood. In this study, we found that perturbation of mitochondrial fission induces a unique mitochondrial uncoupling phenomenon through a large-scale fluctuation of a mitochondrial inner membrane potential. Furthermore, by genetically controlling mitochondrial fission and thereby inducing mild proton leak in mice, we were able to relieve these mice from oxidative stress in a hyperglycemic model. These findings provide mechanistic insight into how mitochondrial fission participates in regulating mitochondrial activity. In addition, these results suggest a potential application of mitochondrial fission to control mitochondrial reactive oxygen species production and oxidative stress in many human diseases.
journal_name
Diabetesjournal_title
Diabetesauthors
Galloway CA,Lee H,Nejjar S,Jhun BS,Yu T,Hsu W,Yoon Ydoi
10.2337/db11-1640subject
Has Abstractpub_date
2012-08-01 00:00:00pages
2093-104issue
8eissn
0012-1797issn
1939-327Xpii
db11-1640journal_volume
61pub_type
杂志文章相关文献
DIABETES文献大全abstract::Integrated closed-loop control (CLC), combining continuous glucose monitoring (CGM) with insulin pump (continuous subcutaneous insulin infusion [CSII]), known as artificial pancreas, can help optimize glycemic control in diabetes. We present a fundamental modular concept for CLC design, illustrated by clinical studies...
journal_title:Diabetes
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.2337/db11-1445
更新日期:2012-09-01 00:00:00
abstract::Cyclic nucleotide phosphodiesterase (PDE) 3B plays an important role in the antilipolytic action of insulin and, thereby, the release of fatty acids from adipocytes. Increased concentrations of circulating fatty acids as a result of elevated or unrestrained lipolysis cause insulin resistance. The lipolytic action of t...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.51.3.631
更新日期:2002-03-01 00:00:00
abstract::Large glucagon immunoreactive substances, extracted from the fetal bovine pancreas and separated by gel filtration in the presence of 6 M guanidinium-hydrochloride, were submitted to lectin-sepharose affinity column chromatograph. Gel-filtered peak I (approximately 45 K delta) and peak II (approximately 10 K delta) in...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diacare.31.11.1002
更新日期:1982-11-01 00:00:00
abstract::It is accepted for insulin-secreting cells in culture that the closure of ATP-sensitive K+ channels causes the glucose-dependent depolarization of pancreatic beta-cells seen at subthreshold levels (less than 100 mg/dl) of glucose. The question remains for the more thoroughly studied beta-cells in freshly dissected int...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.38.4.416
更新日期:1989-04-01 00:00:00
abstract:OBJECTIVE:Malonyl coenzyme A (CoA) decarboxylase (MCD) is a key enzyme responsible for malonyl-CoA turnover and functions in the control of the balance between lipid and glucose metabolism. We utilized RNA interference (siRNA)-based gene silencing to determine the direct role of MCD on metabolic responses in primary hu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-0583
更新日期:2008-06-01 00:00:00
abstract::In vivo studies were undertaken in rats to provide evidence of the neural nature, tentative localization and mode of excitation of the insulin-sensitive central nervous system (CSN) glucoregulator center. In rats under light barbiturate anesthesia minute amounts of insulin injected into the carotid artery resulted in ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.24.4.328
更新日期:1975-04-01 00:00:00
abstract:OBJECTIVE:Melanocortin 3 receptor (MC3R) plays a critical role in weight regulation of rodents, but its role in humans remains unclear. The objective of this study was to identify genetic variants of the MC3R gene and determine its association with childhood obesity. RESEARCH DESIGN AND METHODS:We screened 201 obese c...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-0225
更新日期:2007-10-01 00:00:00
abstract::Insulin signaling in vascular endothelial cells (ECs) is critical to maintain endothelial function but also to mediate insulin action on peripheral glucose disposal. However, gene knockout studies have reached disparate conclusions. Thus, insulin receptor inactivation in ECs does not impair insulin action, whereas ina...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db12-1296
更新日期:2013-05-01 00:00:00
abstract::Diabetes-induced alterations in heme and hemoproteins, as well as its relationship to drug-mediated induction of ALA Synthase (ALA-S), were examined in female Sprague-Dawley rats. Animals were rendered diabetic by a single i.v. injection of streptozotocin (STZ, 65 mg/kg) and measurements were made at various times aft...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.1.37
更新日期:1984-01-01 00:00:00
abstract::A simple, direct assay for T-lymphocyte reactivity to islet antigen(s) in human insulin-dependent diabetes mellitus (IDDM) should facilitate preclinical diagnosis and the evaluation of intervention therapy to avert autoimmune-mediated beta-cell destruction. In subjects with preclinical or clinical IDDM, we measured th...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.40.9.1128
更新日期:1991-09-01 00:00:00
abstract::The apolipoprotein E (apoE) phenotype and allele frequencies were examined in type II (non-insulin-dependent) diabetic patients with normolipidemia (n = 134) and hypercholesterolemia (type IIa hyperlipoproteinemia, n = 35; type IIb hyperlipoproteinemia, n = 42). The frequencies of apoE4-present phenotypes (apoE4/3, ap...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.36.11.1301
更新日期:1987-11-01 00:00:00
abstract::To investigate the pathophysiology of diabetic osteopenia, circulating levels and bone contents of bone gamma-carboxyglutamic acid-containing protein (BGP) were measured in streptozocin-induced diabetic rats . Plasma calcium and total protein were significantly decreased (P less than .01) in the diabetic group, and th...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.6.702
更新日期:1988-06-01 00:00:00
abstract::Glucokinase is a critical component of the physiological glucose sensor found in cell types that are responsive to changes in plasma glucose levels. The acute regulation of glucokinase activity has been shown to occur via a regulatory protein found in liver parenchymal cells (Van Schaftingen E, Detheux M, Da Cunha MV....
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.12.1670
更新日期:1996-12-01 00:00:00
abstract::Perifused islets from rats infused for 7 days with 40% glucose exhibited an altered secretory response to selected stimuli. Both phases of insulin release were blunted when 20 mM L-leucine was tested; the secretory response to a subsequent leucine stimulation was also blunted compared with the control group. The abili...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.40.1.15
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:Insulin resistance and other features of the metabolic syndrome have been causally linked to adipose tissue macrophages (ATMs) in mice with diet-induced obesity. We aimed to characterize macrophage phenotype and function in human subcutaneous and omental adipose tissue in relation to insulin resistance in obe...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0287
更新日期:2010-07-01 00:00:00
abstract::The homeodomain-containing transcription factor pancreatic duodenal homeobox 1 (PDX-1) plays a key role in pancreas development and in beta-cell function. Upstream sequences of the gene up to about -6 kb show islet-specific activity in transgenic mice. Attempts to identify functional regulatory elements involved in th...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diabetes.51.2007.s320
更新日期:2002-12-01 00:00:00
abstract::Production of reactive oxygen species (ROS) is a key instigator of β-cell dysfunction in diabetes. The pleiotropic cytokine interleukin 6 (IL-6) has previously been linked to β-cell autophagy but has not been studied in the context of β-cell antioxidant response. We used a combination of animal models of diabetes and ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db17-1280
更新日期:2018-08-01 00:00:00
abstract::Evidence from animal models suggests that natural killer (NK) cells can be important players in the development of type 1 diabetes, although data in humans are still sparse. We studied the frequency and activation state of blood NK cells at different stages of human type 1 diabetes, and whether genetic or phenotypic N...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0493
更新日期:2007-01-01 00:00:00
abstract::Signaling pathways activated by leptin in metabolically important organs have largely been studied only in animal and/or cell culture studies. In this study, we examined whether leptin has similar effects in human peripheral tissues in vivo, ex vivo, and in vitro and whether the response would be different in lean and...
journal_title:Diabetes
pub_type: 临床试验,杂志文章
doi:10.2337/db14-0625
更新日期:2015-03-01 00:00:00
abstract::A chronic diabetic state was produced in Macaca fuscatus , and these diabetic monkeys were kept without insulin treatment for up to 25 mo. The metabolic derangements were characterized by hyperglycemia, insulinopenia, hyperglucagonemia, ketonemia, and hyperlipidemia. Significant thickening of the capillary basement me...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.33.5.415
更新日期:1984-05-01 00:00:00
abstract:OBJECTIVE:To characterize normal and malformed embryos within the same litters from control and diabetic rats for expression of genes related to metabolism of reactive oxygen species (ROS) or glucose as well as developmental genes. RESEARCH DESIGN AND METHODS:Embryos from nondiabetic and streptozotocin-induced diabeti...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db08-0830
更新日期:2008-12-01 00:00:00
abstract::Previous studies have provided evidence for the transcripts of Cx43 and Cx45 within pancreatic islets. As of yet, however, it has proven difficult to unambiguously demonstrate the expression of these proteins by islet cells. We have investigated whether Cx36, a new connexin species recently identified in mammalian bra...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.5.727
更新日期:2000-05-01 00:00:00
abstract:OBJECTIVE:Reactive oxygen species (ROS) is one of most important factors in impaired metabolism secretion coupling in pancreatic β-cells. We recently reported that elevated ROS production and impaired ATP production at high glucose in diabetic Goto-Kakizaki (GK) rat islets are effectively ameliorated by Src inhibition,...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-0021
更新日期:2011-01-01 00:00:00
abstract::The farnesoid X receptor (FXR) and the liver x receptors (LXRs) are bile acid-activated receptors that are highly expressed in the enterohepatic tissues. The mechanisms that support the beneficial effects of bariatric surgery are only partially defined. We have investigated the effects of ileal interposition (IT), a s...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0299
更新日期:2013-10-01 00:00:00
abstract::Culture of mesangial cells (MCs) in 5.6 vs. 30.0 mmol/l glucose for 3 weeks induced a sustained increase in protein kinase C (PKC) activity, transforming growth factor (TGF)-beta1 mRNA, bioactive TGF-beta, and collagen synthesis. Nitric oxide (NO), generated exogenously by the NO donor S-nitroso-N-acetyl, D,L-penicill...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.46.4.671
更新日期:1997-04-01 00:00:00
abstract:OBJECTIVE:Offspring of mothers with diabetes are at risk of obesity and glucose intolerance in later life. In adults, markers of subclinical inflammation (C-reactive protein [CRP] and interleukin [IL]-6) and endothelial activation (intracellular adhesion molecule [ICAM]-1) are associated with obesity and higher risk fo...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db07-0662
更新日期:2007-11-01 00:00:00
abstract::We performed a variance components linkage analysis of renal function, measured as glomerular filtration rate (GFR), in 63 extended families with multiple members with type 2 diabetes. GFR was estimated from serum concentrations of cystatin C and creatinine in 406 diabetic and 428 nondiabetic relatives. Results for cy...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db06-0781
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:Atherosclerotic cardiovascular disease is the leading cause of death among people with diabetes. Generation of oxidized LDLs and reduced nitric oxide (NO) availability because of endothelial NO synthase (eNOS) dysfunction are critical events in atherosclerotic plaque formation. Biochemical mechanism leading f...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0167
更新日期:2009-10-01 00:00:00
abstract::Because high D-glucose significantly stimulates endothelial cell death, we examined the molecular mechanisms of high D-glucose-induced endothelial apoptosis. Treatment of human aortic endothelial cells with high D-glucose (25 mmol/l), but not mannitol and L-glucose, resulted in a significant decrease in cell number an...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.50.6.1472
更新日期:2001-06-01 00:00:00
abstract::In renal transplant recipients (RTRs), new-onset diabetes after transplantation (NODAT) is a frequent and serious complication limiting survival of graft and patient. However, the underlying pathophysiology remains incompletely understood. In vitro and in preclinical models, HDL can preserve β-cell function, largely b...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db18-1267
更新日期:2019-10-01 00:00:00