Abstract：
:Contrary to extensive researches on the roles of metallothioneins (MTs) in metal tolerance of animals, the roles of plant MTs in metal tolerance are largely under investigation. In this study, we evaluated the functional role of type 2 MT from Colocasia esculenta (CeMT2b) in Zn tolerance of tobacco and E. coli cells. Under Zn-stress conditions, transgenic tobacco overexpressing CeMT2b displayed much better seedling growth, a significant decrease in the levels of H(2)O(2) and an increase in Zn accumulation compared with the wild type. Overexpression of CeMT2b in E. coli greatly enhanced Zn tolerance and Zn accumulation under Zn stresses compared with control cells. CeMT2b bound 5.38 ± 0.29 atoms of Zn per protein. To identify a structural domain of CeMT2b for Zn binding, we investigated the growth of E. coli expressing each of the N-terminal, C-terminal, and central linker domains or a CNC motif deletion from the C-terminus of full-length CeMT2b. The results showed that the CNC motif is required for Zn tolerance, and the N-terminal domain is more effective in Zn tolerance than the C-terminal domain. Taken together, our results provide direct evidence for functional contributions of CeMT2b in Zn tolerance of tobacco and E. coli cells.
journal_name
J Plant Resjournal_title
Journal of plant researchauthors
Kim YO,Lee YG,Patel DH,Kim HM,Ahn SJ,Bae HJdoi
10.1007/s10265-012-0492-8subject
Has Abstractpub_date
2012-11-01 00:00:00pages
793-804issue
6eissn
0918-9440issn
1618-0860journal_volume
125pub_type
杂志文章abstract：:Experimental conditions for efficient callus initiation from mangrove plants were investigated. As a source explant, leaf ofBruguiera sexangula was used. Mangrove plant is one of the most famous woody plants which can grow at the salty area. The initiated callus can be a suitable material for the investigation of salt...
journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/BF02506839
更新日期：1997-03-01 00:00:00
abstract：:Regulation of defense in plants is a complex process mediated by various signaling pathways. Promoter analysis of defense-related genes is useful to understand these signaling pathways involved in regulation. To this end, the regulation of the polygalacturonase-inhibiting protein encoding gene from Vitis vinifera L. (...
journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-012-0515-5
更新日期：2013-03-01 00:00:00
abstract：:Clematis stans is dioecious semi-arboreal, with pale purple-blue, nodding, tubulous flowers in a paniculate inflorescence. Both male and female flowers produce nectar from the base of the calyx tube during a flowering period of 3 or 4 days, and are pollinated by two bumblebee species, Bombus diversus and B. honshuensi...
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pub_type： 杂志文章
doi：10.1007/s10265-002-0046-6
更新日期：2002-10-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1007/s10265-003-0083-9
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-010-0320-y
更新日期：2010-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10265-004-0189-8
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journal_title：Journal of plant research
pub_type： 杂志文章,评审
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更新日期：2017-05-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-003-0085-7
更新日期：2003-06-01 00:00:00
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更新日期：2012-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s10265-014-0661-z
更新日期：2014-11-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-010-0361-2
更新日期：2011-01-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-018-1070-5
更新日期：2019-01-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-011-0433-y
更新日期：2012-03-01 00:00:00
abstract：:Sex allocation of a cosexual wind-pollinated species, Quercus dentata (Fagaceae), was analyzed using biomass, carbon, nitrogen and phosphorus as currencies based on data accumulated for 61 individuals from 1997-2004. Strongly female-biased sex allocation was indicated when measured in terms of biomass and carbon, but ...
journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-005-0206-6
更新日期：2005-06-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章,评审
doi：10.1007/s10265-017-0908-6
更新日期：2017-05-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-015-0712-0
更新日期：2015-07-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-013-0578-y
更新日期：2014-01-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-013-0575-1
更新日期：2013-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2019-09-01 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-006-0056-x
更新日期：2007-03-01 00:00:00
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journal_title：Journal of plant research
pub_type： 已发布勘误
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更新日期：2019-11-01 00:00:00
abstract：:Lavoisiera campos-portoana Barreto (Melastomataceae) has two kinds of leaves, pubescent and glabrous, and branches may have one or both types of leaves at the same moment. The plant is endemic to high altitude rocky fields in Brazil where rainfall is very seasonal. We predicted that these two l...
journal_title：Journal of plant research
pub_type： 杂志文章
doi：10.1007/s10265-011-0416-z
更新日期：2012-01-01 00:00:00
