A R2R3-MYB transcription factor gene in common wheat (namely TaMYBsm1) involved in enhancement of drought tolerance in transgenic Arabidopsis.

Abstract:

:We isolated the TaMYBsm1 genes, encoding R2R3-type MYB proteins in common wheat, aimed to uncover the possible molecular mechanisms related to drought response. The TaMYBsm1 genes, TaMYBsm1-A, TaMYBsm1-B and TaMYBsm1-D, were isolated and analyzed from the common wheat cultivar Shimai 15. Their expression patterns under PEG 6000 and mannitol were monitored by semi-quantitative RT-PCR and β-glucuronidase (Gus) assay. The function of TaMYBsm1-D under drought stress in transgenic Arabidopsis plants was investigated, and the germination rate, water loss rate, as well as the proline and malondialdehyde (MDA) content were compared with that in wild type (WT) plants. The expression of three downstream genes (DREB2A, P5CS1 and RD29A) in TaMYBsm1-D transgenic plants was analyzed. The R2R3-MYB domains of the MYBsm1 proteins were highly conserved in plants. In addition, the TaMYBsm1 proteins were targeted to the nucleus and contained transcriptional activation domains (TADs). Gus assay and semi-quantitative RT-PCR analysis demonstrated that the TaMYBsm1 genes were up-regulated when the wheat was treated by PEG and mannitol. Compared with WT plants, the germination rates were much higher, but the water loss rates were much lower in TaMYBsm1-D overexpression plants. TaMYBsm1-D transgenic plants showed distinct higher proline contents but a lower MDA content than the WT plants. The three downstream genes were highly expressed in TaMYBsm1-D transgenic plants. We concluded from these results that TaMYBsm1 genes play an important role in plant drought stress tolerance through up-regulation of DREB2A, P5CS1 and RD29A. The increase of proline content and decrease of MDA content may also be involved in the drought response.

journal_name

J Plant Res

authors

Li MJ,Qiao Y,Li YQ,Shi ZL,Zhang N,Bi CL,Guo JK

doi

10.1007/s10265-016-0857-5

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1097-1107

issue

6

eissn

0918-9440

issn

1618-0860

pii

10.1007/s10265-016-0857-5

journal_volume

129

pub_type

杂志文章
  • Insight into the basis of root growth in Arabidopsis thaliana provided by a simple mathematical model.

    abstract::Plant organ growth changes under genetic and environmental influences can be observed as altered cell proliferation and volume growth. The two aspects are mutually dependent and intricately related. For comprehensive growth analysis, it is necessary to specify the relationship quantitatively. Here, we develop a simple...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-005-0247-x

    authors: Iwamoto A,Satoh D,Furutani M,Maruyama S,Ohba H,Sugiyama M

    更新日期:2006-03-01 00:00:00

  • Regulation of the grapevine polygalacturonase-inhibiting protein encoding gene: expression pattern, induction profile and promoter analysis.

    abstract::Regulation of defense in plants is a complex process mediated by various signaling pathways. Promoter analysis of defense-related genes is useful to understand these signaling pathways involved in regulation. To this end, the regulation of the polygalacturonase-inhibiting protein encoding gene from Vitis vinifera L. (...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-012-0515-5

    authors: Joubert DA,de Lorenzo G,Vivier MA

    更新日期:2013-03-01 00:00:00

  • Transition from C3 to proto-Kranz to C3-C4 intermediate type in the genus Chenopodium (Chenopodiaceae).

    abstract::The Chenopodiaceae is one of the families including C4 species among eudicots. In this family, the genus Chenopodium is considered to include only C3 species. However, we report here a transition from C3 photosynthesis to proto-Kranz to C3-C4 intermediate type in Chenopodium. We investigated leaf anatomical and photos...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-019-01135-5

    authors: Yorimitsu Y,Kadosono A,Hatakeyama Y,Yabiku T,Ueno O

    更新日期:2019-11-01 00:00:00

  • Occurrence and characterization of PEND proteins in angiosperms.

    abstract::The PEND protein is a DNA-binding protein in the inner envelope membrane of the developing chloroplast. It consists of a short pre-sequence, an N-terminal DNA-binding domain (cbZIP), a central repeat domain, and a C-terminal transmembrane domain. PEND homologs have been detected in various angiosperms, including Arabi...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-005-0200-z

    authors: Terasawa K,Sato N

    更新日期:2005-04-01 00:00:00

  • Anthocyaninless1 gene of Arabidopsis thaliana encodes a UDP-glucose:flavonoid-3-O-glucosyltransferase.

    abstract::We isolated several mutants of Arabidopsis thaliana (L.) Heynh. that accumulated less anthocyanin in the plant tissues, but had seeds with a brown color similar to the wild-type. These mutants were allelic with the anthocyaninless1 (anl1) mutant that has been mapped at 15.0 cM of chromosome 5. We performed fine mappin...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-006-0067-7

    authors: Kubo H,Nawa N,Lupsea SA

    更新日期:2007-05-01 00:00:00

  • Developmental processes of achlorophyllous orchid, Epipogium roseum: from seed germination to flowering under symbiotic cultivation with mycorrhizal fungus.

    abstract::We have achieved the symbiotic cultivation of an apparently achlorophyllous orchid, Epipogium roseum Lindl., with a mycorrhizal fungus isolated from an underground organ of this orchid. Although the seed germination rate was extremely low, subsequent growth from protocorm to flowering was induced in a medium containin...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-006-0044-1

    authors: Yagame T,Yamato M,Mii M,Suzuki A,Iwase K

    更新日期:2007-03-01 00:00:00

  • Structure, function, and evolution of plant NIMA-related kinases: implication for phosphorylation-dependent microtubule regulation.

    abstract::Microtubules are highly dynamic structures that control the spatiotemporal pattern of cell growth and division. Microtubule dynamics are regulated by reversible protein phosphorylation involving both protein kinases and phosphatases. Never in mitosis A (NIMA)-related kinases (NEKs) are a family of serine/threonine kin...

    journal_title:Journal of plant research

    pub_type: 杂志文章,评审

    doi:10.1007/s10265-015-0751-6

    authors: Takatani S,Otani K,Kanazawa M,Takahashi T,Motose H

    更新日期:2015-11-01 00:00:00

  • Chloroplast DNA sequencing and detailed microsatellite genotyping of all remnant populations suggests that only a single genet survives of the critically endangered plant Rehmannia japonica.

    abstract::Rehmannia japonica (Thunb.) Makino ex T. Yamaz. is an endangered perennial herb species in Japan. Although earlier the Japanese considered it a variety of R. glutinosa, recent Japanese taxonomists have consistently regarded it as an independent species. According to the historical literature, Rehmannia japonica seems ...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-016-0873-5

    authors: Kaneko S,Matsuki Y,Qiu YX,Isagi Y

    更新日期:2017-01-01 00:00:00

  • Unique mechanism of plant endocytic/vacuolar transport pathways.

    abstract::The post-Golgi traffic network in plant cells is highly complex, which is correlated with the large number of genes related to this function. RABs and SNAREs are key regulators of tethering and fusion of transport vesicles to target membranes, and the numbers of these regulators have also expanded in plant lineages. I...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-008-0200-x

    authors: Ebine K,Ueda T

    更新日期:2009-01-01 00:00:00

  • A promoter analysis of MOTHER OF FT AND TFL1 1 (JcMFT1), a seed-preferential gene from the biofuel plant Jatropha curcas.

    abstract::MOTHER OF FT AND TFL1 (MFT)-like genes belong to the phosphatidylethanoamine-binding protein (PEBP) gene family in plants. In contrast to their homologs FLOWERING LOCUS T (FT)-like and TERMINAL FLOWER 1 (TFL1)-like genes, which are involved in the regulation of the flowering time pathway, MFT-like genes function mainl...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-014-0639-x

    authors: Tao YB,Luo L,He LL,Ni J,Xu ZF

    更新日期:2014-07-01 00:00:00

  • Correction to: Towards the conservation of the Mesozoic relict fern Christensenia: a fern species with extremely small populations in China.

    abstract::In the original publication of the article, one of the author names, "Tao Fuijwara" was published incorrectly. The correct name is Tao Fujiwara. ...

    journal_title:Journal of plant research

    pub_type: 已发布勘误

    doi:10.1007/s10265-019-01140-8

    authors: Liu H,Schneider H,Yu Y,Fujiwara T,Khine PK

    更新日期:2019-11-01 00:00:00

  • Flowering cycles of woody bamboos native to southern South America.

    abstract::Neotropical woody bamboos range from northern Mexico to southern Argentina and Chile. The most interesting aspect of bamboo biology is their flowering habit. The species that are the most intriguing are those that manifest a cyclic pattern of gregarious flowering after long vegetative periods. The flowering cycle has ...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-013-0593-z

    authors: Guerreiro C

    更新日期:2014-03-01 00:00:00

  • Gamma-tubulin and microtubule organization during microsporogenesis in Ginkgo biloba.

    abstract::This is the first report on gamma-tubulin and microtubule arrays during microsporogenesis in a gymnosperm. Meiosis in Ginkgo biloba is polyplastidic, as is typical of the spermatophyte clade, and microtubule arrays are organized at various sites during meiosis and cytokinesis. In early prophase, a cluster of gamma-tub...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-005-0199-1

    authors: Brown RC,Lemmon BE

    更新日期:2005-04-01 00:00:00

  • Selfing and inbreeding depression in seeds and seedlings of Neobalanocarpus heimii (Dipterocarpaceae).

    abstract::We evaluated the degree of selfing and inbreeding depression at the seed and seedling stages of a threatened tropical canopy tree, Neobalanocarpus heimii, using microsatellite markers. Selection resulted in an overall decrease in the level of surviving selfed progeny from seeds to established seedlings, indicating inb...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-005-0245-z

    authors: Naito Y,Konuma A,Iwata H,Suyama Y,Seiwa K,Okuda T,Lee SL,Muhammad N,Tsumura Y

    更新日期:2005-12-01 00:00:00

  • Camellia nanningensis sp. nov.: the earliest fossil wood record of the genus Camellia (Theaceae) from East Asia.

    abstract::A new species Camellia nanningensis was described on the basis of well-preserved mummified wood from the upper Oligocene Yongning Formation of Nanning Basin in Guangxi Province, South China. This represents the most ancient fossil wood assigned to Camellia, and the earliest fossil record of the family Theaceae in Chin...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-016-0846-8

    authors: Huang LL,Jin JH,Quan C,Oskolski AA

    更新日期:2016-09-01 00:00:00

  • Non-destructive DNA extraction from herbarium specimens: a method particularly suitable for plants with small and fragile leaves.

    abstract::Protocols for DNA extraction from plants generally involve physical and chemical destruction of tissues. Use of these conventional methods precludes preservation of morphological information from herbarium specimens, especially for small plants with few leaves, and reduces the voucher value of specimens. Here, we deve...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-019-01152-4

    authors: Sugita N,Ebihara A,Hosoya T,Jinbo U,Kaneko S,Kurosawa T,Nakae M,Yukawa T

    更新日期:2020-01-01 00:00:00

  • Evolutionary histories and mycorrhizal associations of mycoheterotrophic plants dependent on saprotrophic fungi.

    abstract::Mycoheterotrophic plants (MHPs) are leafless, achlorophyllous, and completely dependent on mycorrhizal fungi for their carbon supply. Mycorrhizal symbiosis is a mutualistic association with fungi that is undertaken by the majority of land plants, but mycoheterotrophy represents a breakdown of this mutualism in that pl...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-020-01244-6

    authors: Ogura-Tsujita Y,Yukawa T,Kinoshita A

    更新日期:2021-01-01 00:00:00

  • Microtubules in early development of the megagametophyte of Ginkgo biloba.

    abstract::Food storage tissue in the seeds of gymnosperms is female gametophyte (megagametophyte) that develops before fertilization, whereas, in seeds of angiosperms, food is stored as endosperm initiated by double fertilization. The megagametophyte is haploid, and endosperm is usually triploid, at least initially. Despite dif...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-008-0161-0

    authors: Brown RC,Lemmon BE

    更新日期:2008-07-01 00:00:00

  • A new method for evaluating flowering synchrony to support the temporal isolation of genetically modified crops from their wild relatives.

    abstract::Hybridization between crops and their wild relatives potentially threatens the genetic identity of the wild plants, particularly in the case of genetically modified crops. Only a few studies have examined the use of temporal isolation to prevent hybridization, and the indices used in those studies, (e.g., the days of ...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-013-0592-0

    authors: Ohigashi K,Mizuguti A,Yoshimura Y,Matsuo K,Miwa T

    更新日期:2014-01-01 00:00:00

  • The evolution of self-compatible and self-incompatible populations in a hermaphroditic perennial, Trillium camschatcense (Melanthiaceae).

    abstract::The evolution of selfing from outcrossing ancestors is known to have occurred repeatedly in angiosperms. Theoretical studies have argued that the transition from outcrossing to selfing is accomplished more easily than the reverse case, and phylogenetic analyses involving self-compatible (SC) and self-incompatible (SI)...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-009-0245-5

    authors: Kubota S,Ohara M

    更新日期:2009-09-01 00:00:00

  • Mechano-sensitive orientation of cortical microtubules during gravitropism in azuki bean epicotyls.

    abstract::The orientation of cortical microtubules (cMT) during gravitropism was studied in epidermal cells of azuki epicotyls. The relative proportion of cells with longitudinal cMT increased in the upper epidermis, and those with transverse cMT increased in the lower epidermis. When epicotyls were kept straight during gravist...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-004-0189-8

    authors: Ikushima T,Shimmen T

    更新日期:2005-02-01 00:00:00

  • Phenology of 16 species of ferns in a subtropical forest of northeastern Taiwan.

    abstract::A knowledge of fern phenology promotes understanding of the biology and ecology of ferns. In this study, the phenology of 16 fern species in a subtropical broadleaf forest (N24 degrees 46', E121 degrees 34') in northeastern Taiwan was monitored from August 1997 to August 2001. Every fern produced both fertile and ster...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-008-0191-7

    authors: Lee PH,Lin TT,Chiou WL

    更新日期:2009-01-01 00:00:00

  • Effect of sodium chloride on the response of the halophyte species Sesuvium portulacastrum grown in mannitol-induced water stress.

    abstract::Sesuvium portulacastrum is a halophytic species well adapted to salinity and drought. In order to evaluate the physiological impact of salt on water deficit-induced stress response, we cultivated seedlings for 12 days, in the presence or absence of 100 mmol l(-1) NaCl, on a nutrient solution containing either 0 mmol l...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-006-0056-x

    authors: Slama I,Ghnaya T,Messedi D,Hessini K,Labidi N,Savoure A,Abdelly C

    更新日期:2007-03-01 00:00:00

  • Genetic guidelines for the conservation of the endangered polyploid Centaurea borjae (Asteraceae).

    abstract::Appropriate management of species of conservation concern requires designing strategies that should include genetic information as small population size and restricted geographic range can reduce genetic variation. We used AFLPs to investigate genetic variation within and among populations of the endangered narrow end...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-012-0497-3

    authors: Lopez L,Barreiro R

    更新日期:2013-01-01 00:00:00

  • Bumblebee-pollination and temporal change of the calyx tube length in Clematis stans(Ranunculaceae).

    abstract::Clematis stans is dioecious semi-arboreal, with pale purple-blue, nodding, tubulous flowers in a paniculate inflorescence. Both male and female flowers produce nectar from the base of the calyx tube during a flowering period of 3 or 4 days, and are pollinated by two bumblebee species, Bombus diversus and B. honshuensi...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-002-0046-6

    authors: Dohzono I,Suzuki K

    更新日期:2002-10-01 00:00:00

  • Molecular cloning and differential expression of an gamma-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus.

    abstract::gamma-Aminobutyrate transaminase (GABA-T) catalyzes the conversion of GABA to succinic semialdehyde. Using differential display PCR and cDNA library screening, a full-length GABA-T cDNA (OsGABA-T) was isolated from rice (Oryza sativa) leaves infected with an incompatible race of Magnaporthe grisea. The deduced amino a...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-006-0018-3

    authors: Wu C,Zhou S,Zhang Q,Zhao W,Peng Y

    更新日期:2006-11-01 00:00:00

  • Pigment compositions are linked to the habitat types in dinoflagellates.

    abstract::Compared to planktonic species, there is little known about the ecology, physiology, and existence of benthic dinoflagellates living in sandy beach or seafloor environments. In a previous study, we discovered 13(2),17(3)-cyclopheophorbide a enol (cPPB-aE) from sand-dwelling benthic dinoflagellates. This enol had never...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-015-0745-4

    authors: Yamada N,Tanaka A,Horiguchi T

    更新日期:2015-11-01 00:00:00

  • Over-expression of Arabidopsis thaliana SFD1/GLY1, the gene encoding plastid localized glycerol-3-phosphate dehydrogenase, increases plastidic lipid content in transgenic rice plants.

    abstract::Lipids are the major constituents of all membranous structures in plants. Plants possess two pathways for lipid biosynthesis: the prokaryotic pathway (i.e., plastidic pathway) and the eukaryotic pathway (i.e., endoplasmic-reticulum (ER) pathway). Whereas some plants synthesize galactolipids from diacylglycerol assembl...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-015-0781-0

    authors: Singh V,Singh PK,Siddiqui A,Singh S,Banday ZZ,Nandi AK

    更新日期:2016-03-01 00:00:00

  • Effects of hypergravity on the elongation growth in radish and cucumber hypocotyls.

    abstract::The elongation growth of the hypocotyls of radish and cucumber seedlings was examined under hypergravity in a newly developed centrifuge (Kasahara et al. 1995). The effects of hypergravity on elongation growth differed between the two species. The rate of elongation of radish hypocotyls was reduced under basipetal hyp...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/BF02344306

    authors: Kasahara H,Shiwa M,Takeuchi Y,Yamada M

    更新日期:1995-03-01 00:00:00

  • Characterization of triterpenoid profiles and triterpene synthase expression in the leaves of eight Vitis vinifera cultivars grown in the Upper Rhine Valley.

    abstract::Plant triterpenoids are a diverse group of secondary metabolites with wide distribution, high chemical diversity and interesting pharmacological and antimicrobial properties. The first step in the biosynthesis of all triterpenoids is the cyclization of the 2,3-oxidosqualene precursor, catalyzed by oxidosqualene cyclas...

    journal_title:Journal of plant research

    pub_type: 杂志文章

    doi:10.1007/s10265-016-0797-0

    authors: Pensec F,Szakiel A,Pączkowski C,Woźniak A,Grabarczyk M,Bertsch C,Fischer MJ,Chong J

    更新日期:2016-05-01 00:00:00